• Title/Summary/Keyword: Suspension system

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이상추출배양을 통한 Botryococcu braunii에서의 탄화수소 생산

  • Sim, Sang-Jun;An, Jin-Yeong;Kim, Byeong-U
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.112-115
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    • 2002
  • The green colonial algae Botryococcus braunii is characterized by unusual high hydrocarbon contents, ranging from 15 to 75% of dry weight, as long-chain unsaturated hydrocarbons. In two-phase bubble column using various organic solvents, poor recovery 08 - 32%) of hydrocarbon seems to be caused by insufficient mixing between two phases, which was operated using only aeration on the narrow interface between hydrophobic solvent and cell suspension. In addition, hydrocarbon was entrapped tightly in cell-matrix (formed by exopolysaccharide) of algal colony, which make difficult to extract using two-phase system. To improve recovery efficiency, mixed-solvent of extractive solvent (octane) and biocompatible solvent (octane) was tested in two-phase column for in situ extraction. In two-phase extraction culture using mixed-solvent, the algal growth was intensely inhibited even at low concentration of polar octanol solvent. the hydrocarbon recovery in two-stage cell-recycle extraction showed a 2.9 fold increase (57%) over that in two-phase extraction. Up to 60 % of hydrocarbon could be recovered without serious cell-damage in the case of downstream separation for 6 h at the high recycle flow rate using this process after batch culture.

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Experimental Study on Physical Characteristics of MR Fluid along Temperature Conditions (온도조건에 따른 MR 유체의 물리 특성에 대한 실험 연구)

  • Lee, Seok-Hyun;Son, June;Baek, Dae-Sung;Kwon, Young-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.3
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    • pp.1247-1252
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    • 2014
  • In the present study, sedimentation and shear stress of MR fluid are investigated to physical characteristics of MR fluid along temperature conditions. MR fluid is a suspension of micrometer-sized magnetic particles in a base liquid. Therefore, dispersion of MR fluid is important in the case of the design and optimization of the system using MR fluid. Due to sedimentation characteristics of MR fluid by magnetic particles, the sedimentation and shear stress of commercial MR fluid are investigated at $25^{\circ}C$ and $80^{\circ}C$ temperatures by using a forced convection oven and a viscometer. From experimental results, the sedimentation and shear stress are more affected by the temperatures of $80^{\circ}C$ than $25^{\circ}C$ and the mixing time of 5min than 10min. Shear stress by the applied current increases the shape of a quadratic equation and are lower 6-18% at $80^{\circ}C$ than $25^{\circ}C$.

A study on the recycle of reused slurry abrasives (CMP 폐슬러리내의 필터링된 연마 입자 재활용에 관한 연구)

  • Kim, Gi-Uk;Seo, Yong-Jin;Park, Sung-Woo;Jeong, So-Young;Kim, Chul-Bok
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07a
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    • pp.50-53
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    • 2003
  • CMP (chemical mechanical polishing) process remained to solve several problems in deep sub-micron integrated circuit manufacturing process. especially consumables (polishing pad, backing film, slurry, pad conditioner), one of the most important components in the CMP system is the slurry. Among the composition of slurries (buffer solution, bulk solution, abrasive particle, oxidizer, inhibitor, suspension, antifoaming agent, dispersion agent), the abrasive particles are important in determining polish rate and planarization ability of a CMP process. However, the cost of abrasives is still very high. So, in order to reduce the high COO (cost of ownership) and COC (cost of consumables) in this paper, we have collected the silica abrasive powders by filtering after subsequent CMP process for the purpose of abrasive particle recycling. And then, we have studied the possibility of recycle of reused silica abrasive through the analysis of particle size and hardness. Also, we annealed the collected abrasive powders to promote the mechanical strength of reduced abrasion force. Finally, we compared the CMP characteristics between self-developed KOH-based silica abrasive slurry and original slurry. As our experimental results, we obtained the comparable removal rate and good planarity with commercial products. Consequently, we can expect the saving of high cost slurry.

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Changes in treatment behavior and cost according to the operation of preventive dentistry in university dental hospital (대학치과병원 예방치과운영 전·후의 환자진료행태와 진료비용 변화)

  • Hong, Hyo-Kyoung;Choi, Seong-Woo
    • Journal of Korean society of Dental Hygiene
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    • v.20 no.5
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    • pp.707-716
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    • 2020
  • Objectives: This study aims to provide basic data for high-quality dental services. In addition, we will promote the operation of preventive dentistry that implements preventive measures. It was conducted to study the change of patient's treatment behavior and treatment cost due to the discontinuation of preventive dentistry in university dental hospitals. Methods: This study collected data using the integrated medical information system of the C University Dental Hospital. From September 1, 2017 to August 31, 2019, data were analyzed using frequency, percentage, mean, standard deviation, chi-square test using SPSS version 24.0 statistical program, and T-test. Results: There was a significant difference in the number of preventive dental treatment cases from 58.3% of preventive dental operation periods to 41.7% of preventive dental operation periods. As a result of comparing the medical expenses, the total medical expenses during the preventive dental operation period decreased from 521,308,872 won to 379,724,995 won during the discontinuation period, 141,583,877 won. The number of medical treatments by treatment behavior decreased 3,835 (28.4%) from a total of 13,520 preventive dental operation periods to 9,685. Conclusions: This study is meaningful as the first study to confirm the change in the treatment behavior and the change in the cost of treatment due to the discontinuation of the operation of preventive dentistry at university dental hospitals. In conclusion, it is thought that there is a possibility of the lack of accessibility and the limitation of professional preventive care due to the discontinuation of preventive dentistry.

Effect of Nutrients on the Production of Extracellular Enzymes for Decolorization of Reactive Blue 19 and Reactive Black 5

  • Lee Yu-Ri;Park Chul-Hwan;Lee Byung-Hwan;Han Eun-Jung;Kim Tak-Hyun;Lee Jin-Won;Kim Sang-Yong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.226-231
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    • 2006
  • Several white-rot fungi are able to produce extracellular lignin-degrading enzymes such as manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase. In order to enhance the production of laccase and MnP using Trametes versicolor KCTC 16781 in suspension culture, the effects of major medium ingredients, such as carbon and nitrogen sources, on the production of the enzymes were investigated. The decolorization mechanism in terms of biodegradation and biosorption was also investigated. Among the carbon sources used, glucose showed the highest potential for the production of laccase and MnP. Ammonium tartrate was a good nitrogen source for the enzyme production. No significant difference in the laccase production was observed, when glucose concentration was varied between 5 g/l and 30 g/l. As the concentration of nitrogen source increased, a lower MnP activity was observed. The optimal C/N ratio was 25 for the production of laccase and MnP. When the concentrations of glucose and ammonium tartrate were simultaneously increased, the laccase and MnP activities increased dramatically. The maximum laccase and MnP activities were 33.7 U/ml at 72 h and 475 U/ml at 96 h, respectively, in the optimal condition. In this condition, over 90% decolorization efficiency was observed.

Analysis and Test for Turn-buckle of Capacity for Measuring Tensile Force (용량별 인장력 측정용 턴버클의 해석 및 실험)

  • Shin, Kyung-Jae;Lee, Swoo-Heon;Lee, Hee-Du
    • Journal of Korean Society of Steel Construction
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    • v.23 no.6
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    • pp.715-724
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    • 2011
  • A turn buckle inserted between tension members that sustain the structural loads in a suspension structure system is a device that is capable of adjusting the tensile force. The tension member is an important element of a tension structure, but no simple and economical method of measuring a tensile force applied to members has been proposed yet. Thus, a turn buckle for measuring the tensile force in a tension member was developed in this study. The turn buckles of the measurement limit loads of 100kN, 200kN, and 300kN were tested through a theoretical analysis and a finite element analysis. There was no significant difference in the results of the theoretical analysis, FEA, and the test. In addition, the ultimate strength of the turn buckle using FEA showed that a new turn buckle is sufficiently safe to use even when there is a five-times overload in the measurement limit load.

Durability Assessment of CFRP Lower Control Arm Using Stress-Life Method (응력수명법을 이용한 탄소섬유강화복합재 로어 컨트롤 아암의 내구성 평가)

  • Jang, Jaeik;Lim, Juhee;Lee, Jongsoo
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.41 no.11
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    • pp.1131-1137
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    • 2017
  • Recently, regulations on fuel efficiency and $CO_2$ emissions have been reinforced in automobile industries. As a result, many companies make an effort to satisfy these regulations by adapting composite materials to the automobile body as well as its components. In particular, the lower control arm in the suspension system is subjected to heavy loads and is designed to be thick to meet operating loads. Therefore, it is essential for the lower control arm to reduce weight and to secure the durability assessment. In this paper, we conducted structural analysis by performing stress and stiffness analysis under given load conditions through finite element analysis, and verified whether it satisfies the load and stiffness conditions. The inertia relief method is adapted to the process of analysis, and the principal stress is used as a criterion for evaluation. Based on these results, the durability assessment is carried out using the stress-life method.

Effect of Herbicide Paraquat on NAD(H)-Redox-cycle (제초제 Paraquat의 NAD(H) 산화환원에 대한 영향)

  • Kim Mi-Lim;Choi Kyung-Ho
    • Journal of Life Science
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    • v.15 no.2 s.69
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    • pp.304-310
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    • 2005
  • This study was carried out to investigate the effect of herbicide paraquat (1,1-dimethyl-4,4-bipyridilium dichloride) on the electron transport system of the cell. When actively growing cells of bacteria were exposed to the 1.0 mM paraquat, more than $50\%$ of the cells were killed at 0 hour. But specific activities of superoxide dismutase (SOD) were not changed at 0 hour of paraquat treatment. Oxido-reductions of NAD (H) by the suspension of bacterial membtane, rat mithochondria and NAD-dependent dehydrogenase were accelerated by paraquat treatment.

Effect of Herbicide Paraquat on Electron Donor and Acceptor (제초제 Paraquat의 전자수용 및 방출에 대한 영향)

  • Kim Mi-Lim;Choi Kyung-Ho
    • Journal of Life Science
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    • v.15 no.2 s.69
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    • pp.311-315
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    • 2005
  • When paraquat was added to the bacterial membrane or mitochondrial suspension, the mixture turned dark blue, but the color was disappeared by aeration. The same phenomenon was seen when electrons were supplied to the paraquat. Blue color appeared from near the cathode, and then spreaded to whole transit system. Coloration was accelerated by addition of alkali, but the color was reduced by addition of acid or oxygen. Paraquat exhibited absorption at ultraviolet region by electron transfer at the concentrations as low as 1.0 mM which did not exert difficulty in showing color reaction. Paraquat caused the increase of the optical density at 340 nm by electron transit, and an aspect of that had a strong resemblance to NADH. The acute toxic action of paraquat seemes to depend on inhibition of energy metabolism cased by paraquat action of electron donor and acceptor.

Characterizations of Modified Silica Nanoparticles(II) ; Preparation and Application of Silica Nanoparticles as a Environmentally Filler

  • Min, Seong-Kee;Bae, Deok-Kwun;Park, Sang-Bo;Yoo, Seong-Il;Lee, Won-Ki;Park, Chan-Young;Seul, Soo-Duk
    • Korean Journal of Materials Research
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    • v.22 no.8
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    • pp.433-438
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    • 2012
  • A chemical process involves polymerization within microspheres, whereas a physical process involves the dispersion of polymer in a nonsolvent. Nano-sized monodisperse microspheres are usually prepared by chemical processes such as water-based emulsions, seed suspension polymerization, nonaqueous dispersion polymerization, and precipitation polymerizations. Polymerization was performed in a four-necked, separate-type flask equipped with a stirrer, a condenser, a nitrogen inlet, and a rubber stopper for adding the initiator with a syringe. Nitrogen was bubbled through the mixture of reagents for 1 hr. before elevating the temperature. Functional silane (3-mercaptopropyl)trimethoxysilane (MPTMS) was used for the modification of silica nanoparticles and the self-assembled monolayers obtained were characterized by X-ray photoelectron spectroscopy (XPS), laser scattering system (LSS), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), elemental analysis (EA), and thermogravimetric analysis (TGA). In addition, polymer microspheres were polymerized by radical polymerization of ${\gamma}$-mercaptopropyl modified silica nanoparticles (MPSN) and acrylamide monomer via precipitation polymerization; then, their characteristics were investigated. From the elemental analysis results, it can be concluded that the conversion rate of acrylamide monomer was 93% and that polyacrylamide grafted to MPSN nanospheres via the radical precipitation polymerization with AAm in ethanol solvent. The microspheres were successfully polymerized by the 'graft from' method.