• Archives of Pharmacal Research
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• 제21권3호
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• pp.278-285
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• 1998

The microdilution assay recommended by NCCLS (National Committee for Clinical Laboratory Standards) is one of the standardized methods of antibiotic susceptibility test. This method has been widely used clinically to obtain MIC values of antibiotics on pathogenic microorganisms. It is more convenient, rapid and simple to test many samples than other test methods such as agar diffusion assay and broth macrodilution assay. The screening of antimicrobial agents from microbial extracts is too laborious in its process. Therefore, a number of screening methods having more simple procedure have been developed. In our laboratory, we applied microdilution assay for screening the antimicrobial agents. This assay showed dose-response results and was more sensitive than disc diffusion assay in our system. We tested 200 samples of microbial extracts originated from 100 microbial strains and selected several samples as potential candidates. In this report, we show that the microdilution assay is more convenient method in screeing of antibiotic susceptibility than those previously reported.

• Corrosion Science and Technology
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• 제10권4호
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• pp.131-135
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• 2011

The demands of high-strength steel have been steadily increased to reduce the weight of vehicles. Although the TRIP steel has been the promising candidate material for the purpose, high strength hinders the application due to the susceptibility to hydrogen delayed fracture in the corrosive environment. Moreover, the testing method was not specified in the ISO standards. In this work, the test method to evaluate the susceptibility of hydrogen delayed fracture was studied by slow strain rate testing technique. The four test experimental parameters were studied : strain rate, hydrogen charging time, holding time after hydrogen charging, and holding time after cadmium plating. The steel was fractured by hydrogen in case the strain rate was in the range of $1{\times}10^{-4}{\sim}5{\times}10^{-7}/sec$. It was confirmed that the slow strain rate test is effective method to evaluate the susceptibility to hydrogen delayed fracture. The holding time over 24 hrs after hydrogen charging, nullified the hydrogen effect, that is, the specimen was no more susceptible to hydrogen after 24 hrs even though the specimen was fully hydrogen-charged. Moreover, cadmium electroplating could not prevent from diffusing out the hydrogen from the steel in the experiment. The effective experimental procedures were discussed.

• 한국임상수의학회지
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• 제20권2호
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• pp.166-171
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• 2003

Milk samples were collected from a total of 418 quarters of 214 Holstein cows. Of these, samples which were positive on California Mastitis Test (CMT) and above 200,000 cells/ml by somatic cell counts were subjected to bacteriological examination and antimicrobial susceptibility test. Major pathogens responsible for mastitis included coagulase-negative staphylococci (34.3%), coagulase-positive staphylococci (21.5%), gran-negative rod (coliforms and noncoliforms: 12.6%) and Streptococcus spp. (8.4%). These strains were tested with 13 antimicrobial agents by the Kirby and Bauer standardized disc diffusion method. The isolated pathogens were mostly susceptible to amoxicillin and cephalothin, but were resistant to erythromycin.

• 한국동물위생학회지
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• 제21권2호
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• pp.141-148
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• 1998

Eighteen strains of Clostpidium perfringens were isolated from the piglets with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxins and susceptibility to antimicrobial agents against the isolates were investigated. 1. The incidence of diarrhea was appeared in 97(22.4%) of 432 piglets examined. 2. The isolation rate of Cl perfingens from the 97 diarrheal faeces were 18.5%(18 strains) 3. The population of Cl perfingens in feces were ranged $10^{8-9}$cfu/g in 5(32.5%) and $10^{3-7}$cfu/g in 13(67.4%) of 18 samples. 4. The toxin type of the 18 isolates investigated by mouse inoculation test was all type C strains of Cl perfringens. 5. As a results of antimicrobial susceptibility test, 18 isolates were higly susceptible to cephalothin, tetracycline and penicillin.

• Journal of Yeungnam Medical Science
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• 제27권1호
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• pp.8-17
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• 2010

It is important to select appropriate antimicrobials for the treatment of infection according to the results of antimicrobial susceptibility tests (ASTs), yet the clinical isolates are sometimes susceptible to antibiotics that are clinically ineffective or this is due to technical error of the ASTs. So, interpretive reading of ASTs is needed and especially for the ${\beta}$-lactams for treating $Enterobacteriacae$. This review describes the interpretive reading of ASTs according to natural antimicrobial resistance and the mechanisms of mechanisms, with giving special attention to the antibiotics phenotypes for $Enterobacteriacae$. Further, as all the diffent tissues have a different antimicrobial concentration for identical antimicrobials, more information is needed on the antimicrobial tissue distribution for the appropriate treatment of infection. (ED note: I hope you send me the paper.)

• Journal of Welding and Joining
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• 제8권3호
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• pp.39-52
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• 1990

The research is to insure the soundness of the stainless steel overlaid weld metal(21/4Cr-IMo steel + SUS 309L) for a pressure vessel application. Detail studies were conducted for the PWHT influence on the micrstructure and intergranular corrosion characteristics of the overlaid weld metal as well as initiation of hydrogen embrittlement crack(or Disbonding) when welded metal are exposed to the hydrogen atmosphere. Hydrogen was experimentally charged to the overlaid weld metal in order to study PWHT effect on the susceptibility of hydrogen embrittlement crack. The results of this research are as follows: 1. At the bond region, austenite grain of the stainless steel side became coarsed and Cr23C6 type carbide was precipitated at the coarsed austenitic grain boundaries. Intergranular Corrosion width(by Straiss test) increased with increasing PWHT temperature and PWHT time.

• 대한수의학회지
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• 제63권3호
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• pp.29.1-29.5
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• 2023

An adult raccoon dog with extensive, deep, and contaminated wounds on the right hip and multiple fractures was rescued. The open wound was managed daily by debridement and flushing for 3 weeks. Modified active drainage was then performed, and antibiotics administered according to the antibiotic susceptibility test. After 2 weeks, the exudate disappeared and the drain was removed. After monitoring for 1 month, the animal was released in to the wild. This case shows that even if infection remains, rapid wound repair is possible if appropriate antibiotic selection through regular examination and active drainage are combined.

• 한국지반환경공학회 논문집
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• 제18권12호
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• pp.37-45
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• 2017

구조물은 영하의 온도가 유지되는 겨울철에 동상으로 인해 크고 작은 피해가 발생한다. 동상은 동토지반의 가장 대표적인 공학적 특성으로 다양한 동상민감성 판정법이 존재한다. 동상민감성 판정법은 크게 3가지로 분류할 수 있다. 첫 번째 동상민감성은 입도분포를 이용하여 결정된다. 많은 국가들이 지반의 입도분포를 이용해 비교적 간편한 동상민감성 판정법을 현장에 적용하고 있다. 그러나 본 논문에서 몇 가지 시료의 입도분포를 이용해 동상민감성 판정 기준에 적용한 결과에 따르면 입도분포로 토질의 동상민감성을 판정하기에는 다소 무리가 있다. 두 번째로는 동상시험에 의한 동상민감성 판정법이다. 새롭게 개발된 온도제어형 삼축셀을 이용한 동상 실내시험을 통해 동상민감성 기준 신뢰성을 분석하였다. 새롭게 개발된 장비와 시험방법은 기존에 제시하고 있는 동상민감성 판정 기준을 충족하는 것으로 판단되었다. 세 번째로는 복합적인 동상민감성 판정법으로 흙의 입도분포, 분류, 그리고 동상시험을 고려한 복합적인 동상민감성 판정 기준 그래프를 제시하고 있다. 본 논문에서 몇 가지 시료에 대해 복합적인 동상민감성 판정 기준에 적용한 결과 다양한 토질에 대한 추가적인 데이터를 바탕으로 복합적인 동상민감성 판정 그래프를 보완해야 할 것으로 판단되었다.

• 한국동물위생학회지
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• 제26권1호
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• pp.57-65
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• 2003

The present study was conducted to examine the outbreak state of bovine dermatophytosis in 14 farms(4 dairy farms, 10 Korean indigenous cattle farms) in Gyeongbuk province from November 2000 to November 2001. The causative agents of dermatophytosis was identified by mycological examination. Antifungal susceptibility test of 26 isolates was performed by agar dilution method, using 5 antifungal drugs. Prevalence of bovine dermatophytosis was found to be 13.5%(90/665) in dairy cattle farms and 14.5%(220/1,520) in Korean indigenous cattle farms. The most common age at which this disease occurred was 2-12 months. This disease usually occurred from winter to spring and the occurrence subsequently decreased in the summer. But 4 Korean indigenous cattle farms with poorly hygienic status were occurred all the year round. The causative agent was identified as Trichophyton verrucosum exclusively in these case. Antifungal susceptibility test of T verrucosum (26 strains) was performed by agar dilution method, using 5 antifungal drugs including tolnaftate, griseofulvin, ketoconazole, amphotericin B and terbinafine. All isolates were highly sensitive to 5 antifungal drugs (geometric mean MICs 0.004∼0.032 $\mu\textrm{g}$/$m\ell$). The isolates were the most sensitive to especially tolnaftate.

• Journal of Welding and Joining
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• 제34권5호
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• pp.54-60
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• 2016

In order to quantitatively evaluate the solidification cracking susceptibility in laser welds of three types of austenitic stainless steels (type 310: A mode, type 316-A: AF mode, type 316-B: FA mode solidifications), the laser beam welding (LBW) transverse-Varestraint tests consisted of multi-mode fiber laser, welding robot and hydraulic pressure system were performed. As the welding speed increased from 1.67 to 40.0 mm/s, the solidification brittle temperature range (BTR) of laser welds for type 316 stainless steels enlarged (316-A: from 37 to 46 K, 316-B: from 14 to 40 K), while the BTR for type 310 stainless steel reduced from 146 to 120 K. In other words, it founds that solidification cracking susceptibility could not be simply mitigated through application of LBW process, and the BTR variation behavior is quite different upon solidification mode of austenitic stainless steels.