• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.25-33
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• 2005

Melanocytes synthesize melanin within discrete organelle termed melanosomes which are transferred to the surrounding keratinocytes and can be produced in varying sizes, numbers and densities. Skin whitening products have become increasingly popular in the past few years. The most successful natural skin whitening agents are: arbutin, vitamin C, kojic acid, and mulberry, which are all tyrosinase inhibitors. In this work, melanoston, a melanogenesis inhibitor isolated from yeast was studied to understand its mechanism of melanogenesis inhibition. It was found that melanoston was not a tyrosinase inhibitor, while when melanoston was applied to the B16 melanoma cell culture media, the intracellular tyrosinase activity was decreased by more than $30\%$. When B16 melanoma was stimulated with $\alpha$-MSH, cell morphololgy was dramatically changed to have lots of dendrites on the cell membrane surface. On the other hand, B16 was treated with $\alpha$-MSH and melanoston, simultaneously, the change of cell morphologv was not so great. This inhibitory effect of melanoston was found to be related to the inhibition of intracellar activation and transportation of tyrosinase, which was observed by irmmunostaining of B16 melanoma using anti-tyrosinase antibody. From these results, melanoston was regarded as an inhibitor to the differentiation of melanoma cells.

• The Journal of the Acoustical Society of Korea
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• v.40 no.5
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• pp.408-416
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• 2021

In the ultrasonic dispersion, in order to avoid direct contact of the radiation surface of ultrasonic transducers with a liquid sample, the liquid sample is separated by a glass container and it receives ultrasonic energy through an acoustic medium. The transmission efficiency of the ultrasonic energy in the multi-layered ultrasonic system is an important factor. In this study, we suggested a method that can improve the ultrasonic energy transfer efficiency by using a propylene glycol solution as a liquid matching layer in the multi-layered acoustic system. In this method, a propylene glycol solution was filled between the Langevin-type ultrasonic transducer and the luminol solution and the sonoluminescence phenomena in the luminol solution, which is caused by nonlinear effect of high power ultrasound radiated from the transducer, was examined by using a Photo Multiplier Tube (PMT). The transmission efficiency depending on the concentration of propylene glycol solution was observed, and we can see that as the concentration of the propylene glycol solution increased, the matching effect increased while the acoustic attenuation increased. It was confirmed that there is an optimal concentration compromised these two conflicting conditions, and the optimum concentration of the propylene glycol solution was determined experimentally.

• Journal of the Korean Society of Radiology
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• v.15 no.2
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• pp.165-171
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• 2021

To prevent the secondary hospital-acquired infection (cross-infection) from occurring in the general radiographic room in the department of radiology, the microbial measurement was conducted at the points making direct close contact with radiologists and patients. For the case of radiologists, the microbial measurement and incubation were focused on the x-ray tube handle of the radiation generating device, and, for the case of patients, the microbial measurement and incubation were focused on the chin supporting device, chest-contact point, and handle. Once disinfected with Aniosurf, the sterilized media were gathered and identified, and the microorganisms were confirmed. Based on the identification results, it was confirmed that the points making direct close contact with radiologists showed a value of 103 CFU for Proteus mirabilis, Staphylococcus epidermidis, Bacillus spp. and Candida spp., and that the points making direct close contact with patients showed a value of 103~5 CFU for Proteus mirabilis, Enterococcu faecium, Pseudomonas aeruginosa, NTM(Non-Tuberculosis Mycobacteria) and Candida spp.. It was also confirmed that the types and number of microorganisms gathered from the points making direct close contact with patients were greater. Fortunately, most of the involved microorganisms were observed to be on the skin surface and are known to become extinct when disinfected in accordance with the hospital-acquired infection control rules. However, since even minor exposure to such microorganisms may be lethal for patients with reduced immunity, caution must be taken. In particular, since the points making contact with patients showed a high level of microbial measurement, it was thought that it would be necessary for radiologists and personnel having frequent access to strictly disinfect the parts, such as instruments and handles, making frequent contact with patients. The purpose of this study was to announce the importance of safe microbial control in the radiographic inspection room in hospital, and this study is expected to be used as the baseline data for preventing hospital-acquired secondary infection and Nth infectious diseases.

• Korean Journal of Remote Sensing
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• v.35 no.2
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• pp.203-215
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• 2019

As hyperspectral sensors that can be mounted on drones are developed, it is possible to acquire hyperspectral imagery with high spatial and spectral resolution. Although the importance of atmospheric correction has been reduced since imagery of drones were acquired at a low altitude,studies on the conversion process from raw data to spectral reflectance should be done for studies such as estimating the concentration of surface materials using hyperspectral imagery. In this study, a vicarious radiometric calibration and an atmospheric correction algorithm based on atmospheric radiation transfer model were applied to hyperspectral data of drone and the results were compared and analyzed. The vicarious calibration method was applied to an empirical line calibration using the spectral reflectance of a tarp made of uniform material. The atmospheric correction algorithm used ATCOR-4 based Modran-5 that was widely used for the atmospheric correction of aerial hyperspectral imagery. As a result of analyzing the RMSE of the difference between the reference reflectance and the correction, the vicarious calibration using the tarp in a single period of hyperspectral image was the most accurate, but the atmospheric correction was possible according to the application purpose of using hyperspectral imagery. If the correction process of normalized spectral reflectance is carried out through the additional vicarious calibration for imagery from multiple periods in the future, accurate analysis using hyperspectral drone imagery will be possible.

• Journal of Gastric Cancer
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• v.21 no.2
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• pp.191-202
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• 2021

Purpose: A near-infrared (NIR) fluorescence imaging is a promising tool for cancer-specific image guided surgery. Human epidermal receptor 2 (HER2) is one of the candidate markers for gastric cancer. In this study, we aimed to synthesize HER2-specific NIR fluorescence probes and evaluate their applicability in cancer-specific image-guided surgeries using an animal model. Materials and Methods: An NIR dye emitting light at 800 nm (IRDye800CW; Li-COR) was conjugated to trastuzumab and an HER2-specific affibody using a click mechanism. HER2 affinity was assessed using surface plasmon resonance. Gastric cancer cell lines (NCI-N87 and SNU-601) were subcutaneously implanted into female BALB/c nu (6-8 weeks old) mice. After intravenous injection of the probes, biodistribution and fluorescence signal intensity were measured using Lumina II (Perkin Elmer) and a laparoscopic NIR camera (InTheSmart). Results: Trastuzumab-IRDye800CW exhibited high affinity for HER2 (K_D=2.093(3) pM). Fluorescence signals in the liver and spleen were the highest at 24 hours post injection, while the signal in HER2-positive tumor cells increased until 72 hours, as assessed using the Lumina II system. The signal corresponding to the tumor was visually identified and clearly differentiated from the liver after 72 hours using a laparoscopic NIR camera. Affibody-IRDye800CW also exhibited high affinity for HER2 (K_D=4.71 nM); however, the signal was not identified in the tumor, probably owing to rapid renal clearance. Conclusions: Trastuzumab-IRDye800CW may be used as a potential NIR probe that can be injected 2-3 days before surgery to obtain high HER2-specific signal and contrast. Affibody-based NIR probes may require modifications to enhance mobilization to the tumor site.

• Journal of the Korean earth science society
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• v.41 no.6
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• pp.575-587
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• 2020

Meteorological phenomena are observed by the Korea Meteorological Administration in a variety of ways (e.g., surface, upper-air, marine, ocean, and aviation). However, there are limits to the meteorological observation of the planetary boundary layer (PBL) that greatly affects human life. In particular, observations using a sonde or aircraft require significant observational costs in economic terms. Therefore, the goal of this study was to measure and analyze the meteorological factors of the vertical distribution of the see-land breeze among local meteorological phenomena using meteorological drones. To investigate the spatial distribution of the see-land breeze, a same integrated meteorological sensor was mounted on each drone at three different points (seaside, bottom of mountain, and mountainside), including the Boseong tall tower (BTT) at the Boseong Standard Weather Observatory (BSWO) in the Boseong region. Vertical profile observations for air temperature, relative humidity, wind direction, wind speed, and air pressure were conducted up to 400 m every 30 minutes from 1100 LST to 1800 LST on August 4, 2018. The spatial characteristics of meteorological phenomena for temperature, relative humidity, and atmospheric pressure were not shown at the four points. Strong winds (~8 m s-1) were observed from the midpoint (~100 m) at strong solar radiation hour, and in the afternoon the wind direction changed from the upper layer at the inland area to the west wind. It is expected that the analysis results of the lower atmospheric layer observed using the meteorological drone may help to improve the weather forecast more accurately.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.21 no.3
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• pp.62-72
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• 2022

In this study, a miniaturized GPS band(L₁ : 1.575 GHz) antenna that can be mounted on a small drone is proposed. The miniaturization was designed by applying the perturbation method based on the λ/4 microstrip antenna and lengthening the current path at the edge of the patch. The miniaturized antenna was fabricatred such that it could be attached to the surface of styrofoam(ε_r=1.06, t=10 mm) having a size of 10 mm × 9 mm × 10 mm (0.05 λ × 0.05 λ × 0.05 λ). The thickness and length of the feeding line and the spacing between short stubs were adjusted for impedance matching. S₁₁ was found to be -18.8 dB at the center frequency of the fabricated antenna, 1.575 GHz. The radiation pattern measurement results show that the maximum gain of E_θ is 1.87 dBi in 0 directions in the xz-plane, and that E_θ is an omnidirectional characteristic with an average gain of -1.7 dBi in the yz-plane. It was found that the antenna can be used as an ultra-small microstrip antenna, which can be mounted on a small dron for GPS, and is capable of preserving a reduction ratio of 98.8% as compared to a λ/2 microstrip patch antenna.

• Journal of the Korean Society of Radiology
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• v.17 no.4
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• pp.573-579
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• 2023

This study compares dose difference between the presence or absence of grid in Chest PA radiography using auto exposure control and compares image quality among presence, absence or virtual grid, and proposes a new clinically useful grid combination for chest radiography. The human body phantom was placed Chest PA position and the dosimeter was placed at T6. The same irradiation conditions and field size were applied. 30 images were obtained in the state in which grid was applied and in the state in which grid was not applied, and an additional 30 images in which the virtual grid was applied to the image without the grid were obtained. Radiation dose was presented to entrance surface dose. The image quality was analyzed by comparing the signal-to-noise and contrast-to-noise ratio. ESD decreased by 48% when the grid was not used, compared to when the grid was used. SNR and CNR increased by 32% and 30% compared to grid use when grid was not used, respectively. In the case of using the virtual grid, it increased by 18% and 16% respectively, compared to the case of using the grid. As a result of this study, it is believed that when using a virtual grid instead of a grid, the quality of the image can be maintained while reducing the patient dose.

• Journal of Life Science
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• v.33 no.7
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• pp.593-604
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• 2023

The ocean accounts for over 70% of the Earth's surface and is a space of largely unexplored unknowns and opportunities. Korea is a peninsula surrounded by the sea on three sides, emphasizing the importance of marine research. The ocean has an extremely complex environment with immense biological diversity. In terms of microbiology, the marine environment has varying factors like extreme temperature, pressure, solar radiation, salt concentration, and pH, providing ecologically unique habitats. Due to this variety, marine organisms have very different phylogenetic classifications compared with terrestrial organisms. Although various microorganisms inhabit the ocean, studies on the diversity, isolation, and cultivation of marine microorganisms and the secondary metabolites they produce are still insufficient. Research on bioactive substances from marine microorganisms, which were rarely studied until the 1990s, has accelerated in terms of natural products from marine Actinomycetes since the 2000s. Since then, industries for bioplastic and biofuel production, carbon dioxide capture, probiotics, and pharmaceutical discovery and development of antibacterial, anticancer, antioxidant, and anti-inflammatory drugs using bacteria, archaea, and algae have significantly grown. In this review, we introduce current research findings and the latest trends in life science and biotechnology using marine microorganisms. Through this article, we hope to create consumer awareness of the importance of basic and applied research in various natural product-related discovery fields other than conventional pharmaceutical drug discovery. The article aims to suggest pathways that may boost research on the optimization and application of future marine-derived materials.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.37 no.4
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• pp.394-399
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• 2024

An irradiator is developed using two UVA wavelength ranges of SMD LEDs as a curing light source. This module has dimensions of 545×111×300 mm³ and is equipped with a TIR bar-shaped lens made of PDMS silicone resin. The developed irradiator offers high uniformity, with 89% in the centerline of the horizontal axis direction, for two different wavelength ranges of 365 nm and 385 nm. The radiation intensity from the light source module shows highly directional characteristics, and the irradiator provides a maximum irradiance of 1,634 mW/cm² at a working distance of 50 mm. During the initial 5 minutes of operation, the irradiance experiences a rapid decrease. However, this issue is addressed by optimizing the LED's current reduction characteristics and managing the Transistor's temperature rise in the constant current circuit. After continuous operation for approximately 60 minutes. The highest temperature, near the central part of the irradiating surface, reaches 69.7℃, while the lowest temperature, near the edges, is 41.1℃.