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Gas Permeation Characteristics of PEBAX-PEI Composite Membranes Containing ZIF-8 Modified with Amine (Amine으로 개질된 ZIF-8을 함유한 PEBAX-PEI 복합막의 기체투과 특성)

  • Yi, Eun Sun;Hong, Se Ryeong
    • Applied Chemistry for Engineering
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    • v.31 no.6
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    • pp.679-687
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    • 2020
  • In this study, poly(ether-block-amide) (PEBAX)/zeolitic imidazolate framework-8 (ZIF-8)-polyetherimide (PEI), and PEBAX/amine-modified ZIF-8 (amineZIF-8)-PEI composite membranes were prepared by varying the contents of ZIF-8 and amineZIF-8. Also the gas permeability properties of N2 and CO2 were investigated for each composite membrane. The N2 and CO2 permeability of the PEBAX/ZIF-8-PEI composite membrane increased as the ZIF-8 content increased, while the CO2/N2 selectivity gradually decreased. In the case of the PEBAX/amineZIF-8-PEI composite membrane, the permeability of N2 decreased slightly, and CO2 increased till amineZIF-8 amount was 0.5 wt% and then decreased when the content increased further. The CO2/N2 selectivity was the highest with a value of 78.3 at 0.5 wt% of amineZIF-8. because the amine modification in the amineZIF-8 improves the compatibility between PEBAX and amineZIF-8, It seems that amineZIF-8 was evenly dispersed in PEBAX, which could be greatly influenced by the porousity of ZIF-8 and also the affinity of amine toward CO2.

HFN-Based Right Management for IoT Health Data Sharing (IoT 헬스 데이터 공유를 위한 HFN 기반 권한 관리)

  • Kim, Mi-sun;Park, Yongsuk;Seo, Jae-Hyun
    • Smart Media Journal
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    • v.10 no.1
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    • pp.88-98
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    • 2021
  • As blockchain technology has emerged as a security issue for IoT, technology which integrates block chain into IoT is being studied. In this paper is a research concerning token-based IoT service access control technology for data sharing, which propose a possessor focused data sharing technic by using the permissioned blockchain. To share IoT health data, a Hyperledger Fabric Network consisting of three organizations was designed to provide a way to share data by applying different access control policies centered on device owners for different services. In the proposed system, the device owner issues access control tokens with different security levels applied to the participants in the organization, and the token issue information is shared through the distributed ledger of the HFN. In IoT, it is possible to lightweight the access control processing of IoT devices by granting tokens to service requesters who request access to data. Furthmore, by sharing token issuance information among network participants using HFN, the integrity of the token is guaranteed and all network participants can trust the token. The device owners can trust that their data is being used within their authorized rights, and control the collection and use of data.

Experimental study on vertically upward steam-water two-phase flow patterns in narrow rectangular channel

  • Zhou, Jiancheng;Ye, Tianzhou;Zhang, Dalin;Song, Gongle;Sun, Rulei;Deng, Jian;Tian, Wenxi;Su, G.H.;Qiu, Suizheng
    • Nuclear Engineering and Technology
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    • v.53 no.1
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    • pp.61-68
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    • 2021
  • Experiments of vertically upward steam-water two-phase flow have been carried out in single-side heated narrow rectangular channel with a gap of 3 mm. Flow patterns were identified and classified through visualization directly. Slug flow was only observed at 0.2 MPa but replaced by block-bubble flow at 1.0 MPa. Flow pattern maps at the pressure of 0.2 MPa and 1.0 MPa were plotted and the difference was analyzed. The experimental data has been compared with other flow pattern maps and transition criteria. The results show reasonable agreement with Hosler's, while a wide discrepancy is observed when compared with air-water two-phase experimental data. Current criteria developed based on air-water experiments poorly predict bubble-slug flow transition due to the different formation and growth of bubbles. This work is significant for researches on heat transfer, bubble dynamics and flow instability.

MicroRNA-21 promotes epithelial-mesenchymal transition and migration of human bronchial epithelial cells by targeting poly (ADP-ribose) polymerase-1 and activating PI3K/AKT signaling

  • Zhang, Shiqing;Sun, Peng;Xiao, Xinru;Hu, Yujie;Qian, Yan;Zhang, Qian
    • The Korean Journal of Physiology and Pharmacology
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    • v.26 no.4
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    • pp.239-253
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    • 2022
  • Epithelial-mesenchymal transition (EMT) is known to be involved in airway remodeling and fibrosis of bronchial asthma. However, the molecular mechanisms leading to EMT have yet to be fully clarified. The current study was designed to reveal the potential mechanism of microRNA-21 (miR-21) and poly (ADP-ribose) polymerase-1 (PARP-1) affecting EMT through the PI3K/AKT signaling pathway. Human bronchial epithelial cells (16HBE cells) were transfected with miR-21 mimics/inhibitors and PARP-1 plasmid/small interfering RNA (siRNA). A dual luciferase reporter assay and biotin-labeled RNA pull-down experiments were conducted to verify the targeting relationship between miR-21 mimics and PARP-1. The migration ability of 16HBE cells was evaluated by Transwell assay. Quantitative real-time polymerase chain reaction and Western blotting experiments were applied to determine the expression of Snail, ZEB1, E-cadherin, N-cadherin, Vimentin, and PARP-1. The effects of the PI3K inhibitor LY294002 on the migration of 16HBE cells and EMT were investigated. Overexpression of miR-21 mimics induced migration and EMT of 16HBE cells, which was significantly inhibited by overexpression of PARP-1. Our findings showed that PARP-1 was a direct target of miR-21, and that miR-21 targeted PARP-1 to promote migration and EMT of 16HBE cells through the PI3K/AKT signaling pathway. Using LY294002 to block PI3K/AKT signaling pathway resulted in a significant reduction in the migration and EMT of 16HBE cells. These results suggest that miR-21 promotes EMT and migration of HBE cells by targeting PARP-1. Additionally, the PI3K/AKT signaling pathway might be involved in this mechanism, which could indicate its usefulness as a therapeutic target for asthma.

Development and Evaluation of Home Economics Flipped Problem-Based Learning(FPBL) Education Plans for Middle School Students: Focusing on 'Food Selection and Storage' Unit (중학생을 위한 가정과 거꾸로 문제중심학습(FPBL) 교육안 개발과 평가: 식품 선택과 보관 단원을 중심으로)

  • Ryu, Ji Sun;Chae, Jung-Hyun
    • Journal of Korean Home Economics Education Association
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    • v.33 no.4
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    • pp.65-84
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    • 2021
  • The purpose of this study was to develop and evaluate the Home Economics(HE) Flipped Problem-Based Learning(FPBL) education plans focusing on 'food selection and storage' unit for middle school students. The results of this study are as follows. First, middle school students who participated in the class had mainly experienced lecture-style classes previously, but they preferred group activity classes to lecture-style classes. Their 'preferred on-line class tools' was 'Miricanvas', and the 'helpful on-line class tools for learning' was 'Tinkerbell'. Second, the HE FPBL education plan was designed and developed to conduct block time classes, twice a week for 3 weeks by applying the '13 stages of FPBL'. The main topic of the class is "food selection and storage that protects health and the environment". The practical and unstructured problems in the FPBL was to participate in the 'Food Selection and Storage to Protect Health and Environment' mission development contest of a TV entertainment program. Learning materials(stepping video, reading materials, activity sheets, and evaluation tools for process-based evaluation) were developed. The 206 senior students at a middle school in Haeundae-gu, Busan, took the class for three weeks and evaluated it as a good class that helps them learn, is satisfactory, interesting, and suitable, leads to class participation, and is differentiated from other teaching methods.

Dietary effects of protected fat, glycerol, and soybean meal on performance, physiological parameters, carcass characteristics, and behavioral measurements of late-fattening Hanwoo steers under heat stress conditions

  • Jun Sik Woo;Gyeong Rim Ryu;Jeong Hoon Kim;Sun Sik Jang;Hong Gu Lee;Keun Kyu Park
    • Animal Bioscience
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    • v.37 no.7
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    • pp.1303-1315
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    • 2024
  • Objective: This study aimed to determine the effects of increasing energy and protein levels in diets by including protected fat (PF), glycerol (GL), and soybean meal (SBM) on growth performance, physiological parameters, carcass characteristics, and behavioral measurements of late-fattening Hanwoo steers under heat stress conditions. Methods: Thirty-six steers (initial body weight, 724.9±58.3 kg; age, 25.5±0.4 month) were assigned into control (total digestible nutrient [TDN] 76%, crude protein [CP] 15%), PF (TDN 83.6%, CP 15%), PF+GL (TDN 83.6%, CP 15%) and PF+GL+SBM (TDN 83.6%, CP 16.5%) by randomized complete block design for a total of 16 weeks with division of 4-week periods. The average temperature-humidity index was 87.0 (1st period; severe), 82.8 (2nd; moderate), 71.4 (3rd; comfort), and 68.1 (4th; comfort). Results: The dry matter intake (DMI) showed no treatments differences during the whole experiment. However, DMI in 1st and 2nd period decreased by approximately 30% and 10% compared to 4th period, respectively. Higher average daily gain and feed conversion ratio were noted for treatments compared to control at both 1st and 2nd period (p<0.05). There were no treatment effects on rectal temperature (RT), cortisol, and behaviors during the entire experiment. However, both RT and cortisol in 0, 1st and 2nd period were higher than those of 3rd and 4th period (p<0.05). Carcass yield and grade remained unaffected by increasing TDN and CP levels. Behavioral changes in the hot season (1st period) included reduced lying (43%), increased standing (48%), decreased walking (62%), and decreased eating (38%) (p<0.05), with an increase in drinking by 54%. Rumination during standing was 53% higher, while rumination during lying was about 33% lower compared to the post-hot season (3rd period) (p<0.05). Conclusion: Dietary supplementation of protected fat in late-fattening Hanwoo steers under heat stress had a positive effect on preventing a reduction in performance.

Effects of Total Mixed Rations with Corn on Growth and Meat Quality of Castrated Korean Black Goats (옥수수 첨가 TMR 사료 급여가 거세흑염소의 발육과 육질에 미치는 영향)

  • Kim, Sang-Uk;Choi, Young-Sun;Yoo, Dae-Joong;Ku, Min-Jung;Lee, Gi-Ho;Park, Sang-Guk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.4
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    • pp.350-356
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    • 2016
  • The objective of this study was to evaluate the effect of different dietary corn levels in Total Mixed Rations (TMR) on growth and meat quality of castrated Korean black goats. For feeding trials, thirty-two castrated Korean black goats with similar age (average of 3 months) and body weight (average of 15.9 kg) were divided into the following four treatment groups: commercial feed (control), TMR containing 15% of corn (T1), TMR containing 25% of corn (T2), and TMR containing 35% of corn (T3) in a randomized complete block design. Final body weight was the highest in T2 (58.9 kg), followed by that in control group (53.1 kg). Average daily gain (ADG) and total dry matter intake (TDMI) were also the highest in T2 (100.2 g and 1,346 g, respectively). However, feed conversion ratio (13.4) was the lowest in T2. Carcass weight and dressing percentage in T2 and T3 were higher than those in the control. For meat quality characteristics, crude fat content was the highest in T2 (6.54%). Cooking loss (15.2%) was the lowest in the control. However, the control had the highest flavor value (5.3%). Oleic acid (C18:l) and palmitic acid (C16:0) were the highest in T2 (48.6% and 22.4%, respectively). Stearic acid (C18:0) was the highest in T1 (14.5%). Unsaturated fatty acid was the highest in the control (58.8%), followed by that in T3 (58.2%), T2 (57.8%), and T1 (54.4%). These results indicate that TMR containing 25% of corn could improve the growth performance and meat quality of castrated Korean black goats.

Soil Microbial Flora and Chemical Properties as Influenced by the Application of Pig Manure Compost (돈분퇴비의 시용이 배추재배지 토양의 미생물상 및 화학성에 미치는 영향)

  • Weon, Hang-Yeon;Kwon, Jang-Sik;Suh, Jang-Sun;Choi, Woo-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.1
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    • pp.76-83
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    • 1999
  • Studies were conducted during 2 months from May of 1997 to evaluate the effects of pig manure compost(PMC) on soil microbial flora. To do so, a field experiment of Chinese cabbage(Brassica campestris L.) was conducted in a randomized block design on a sandy loam soil and microbial floral characteristics in soils were analyzed. Treatments to control included the application of PMC at (A) $8Mg\;ha^{-1}$CM-8), (B) $29Mg\;ha^{-1}$(CM-2,9), and (C) $57Mg\;ha^{-1}$(CM-57), and of chemical fertilizer(D) at $320N-80P_2O_5-200K_2O\;kg\;ha^{-1}$(NPK). In each treatment, the rhizosphere and non-rhizosphere soils were tested for the analysis of microbial populations. The populations of bacteria, actinomycetes, and fungi increased in soils with the applications of PMC and chemical fertilizer, but that of Bacillus sp. decreased. However, the population of fluorescent Pseudomonas sp. was reduced in NPK plots only. With increasing application rates of PMC, the number of colony forming units(cfu) of bacteria (Pseudomonas sp. and actinomycetes) and fungi increased. in all PMC-treated plots, the population density peaked at early growth stage for bacteria(including Bacillus sp.), at late growth for fluorscent Pseudomonas sp., and at harvest for fungi and actinomycetes. The rhizosphere effect was greatest for fluorscent Pseudomonas sp. As the application rates of PMC increased, Total N, organic matter, available phosphate, and exchangeable -K, -Ca, and -Mg increased compared to control, but soil pH was lowered. In NPK plots, EC was 3.4-fold and exchangeable K was 5-fold higher than control.

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Study of heat transfer to the implant-bone interface induced by grinding of occlusal surface of implant gold prosthesis (금 합금 보철물의 교합면 삭제로 인한 임플란트-골 계면으로의 열전달에 관한 연구)

  • Jo, Jae-Young;Kang, Sun-Nyo;Jeong, Chang-Mo;Yun, Mi-Jung;Huh, Jung-Bo;Jeon, Young-Chan
    • The Journal of Korean Academy of Prosthodontics
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    • v.50 no.1
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    • pp.29-35
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    • 2012
  • Purpose: The purpose of this study was to analyze temperature change along the implant-bone interface induced by grinding the occlusal surface of implant gold prosthesis and to compare the temperature generated by grinding of prosthesis with different cooling methods. Materials and methods: The experimental gold prostheses were fabricated with dental gold alloy and castable abutment. The prostheses had 3 cylindrical protrusions on the occlusal surface with 1mm in height. Temperature was measured using 16 thermocouple wires attached to the implant fixture surface and the fixture was embedded in an acrylic resin block inside the $37^{\circ}C$ water bath. Cylinders were grinded for a period of 30 second with a low-speed handpiece with green stone point. One cylindrical protrusion was grinded without cooling, the second one was grinded with air blow, and the third one was grinded with water-spray. Results: The mean maximum temperature was measured more than $47^{\circ}C$ of the implant and the maximum temperature was measured at the cervical portion of the implant in the group without cooling. There was statistically significant difference between the group without cooling and the groups with cooling (P<.05). However, there was no significant difference at all portion of implant in the groups with cooling (P>.05). Conclusion: The results of this study support that the grinding of implant gold prosthesis without cooling may damage the peri-implant tissue. The continuous use of air blow and water-spray adjacent to prosthesis during the grinding of implant gold prosthesis may prove to be beneficial for cooling of the implant.

1-Methylcyclopropene and Carbon Dioxide Absorber Reduce Chilling Injury of Eggplant ($Solanum$ $melongena$ L.) during MAP Storage (1-Methylcyclopropene과 이산화탄소 제거제 처리에 의한 가지($Solanum$ $melongena$ L.) 과실의 MAP 저장 중 저온장해 경감)

  • Veasna, Hay;Hwang, Yong-Soo;Choi, Jong-Myung;Ahn, Young-Jik;Lim, Byung-Sun;Chun, Jong-Pil
    • Journal of Bio-Environment Control
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    • v.21 no.1
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    • pp.50-56
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    • 2012
  • This study was conducted to extend the postharvest life of eggplant ($Solanum$ $melongena$ L.) by modified atmosphere packaging using a OPP bag with 1-MCP to evaluate the alleviation effects of that treatment on external chilling injury during at low temperature. 1-MCP treatment at the level of $1.0{\mu}L{\cdot}L^{-1}$ were very effective in delaying the progress of surface bronzing of eggplant during 10 days of storage at $8^{\circ}C$, but not significant at $1^{\circ}C$. Also, the treatment of 1-MCP at the levels of $0.1{\sim}1.0{\mu}L{\cdot}L^{-1}$ are beneficial to complement low temperature storage and maintain quality indices such as higher firmness and lower weight loss than untreated control even at a critical low temperature 4 to $8^{\circ}C$. The treatment of carbon dioxide using dry ice (DI, 5 g/3 fruits) significantly promoted chilling injury of eggplant stored at $4^{\circ}C$, symptoms were appeared from 3 days after treatment and combination treatment of 1-MCP did not block the development of chilling injury symptom. Meanwhile, single treatment of $CO_2$ absorber (CA, 5 g/3 fruits) or combination treatment with 1-MCP at the level of $1.0{\mu}L{\cdot}L^{-1}$ prevented effectively the external and internal chilling injury at $4^{\circ}C$ during 6 days and 15 days of MAP storage, respectively. Results suggest that 1-MCP and carbon dioxide absorber treatments delay fruit deterioration and are beneficial to broaden storage temperature of eggplant fruits.