• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.115-121
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• 2011

hFSH is a glycoprotein secreted from anterior pituitary and consists of ${\alpha}$ and ${\beta}$ subunits. Because of its major biological functions including sperm formation in the male and for follicular growth, FSH is used to cure woman's sterility. In this study we tried to produce recombinant hFSH in vitro using a retrovirus expression vector. Two major components of the vector we constructed are: ( i ) a DNA fragment containing ${\alpha}$ and ${\beta}$ genes fused by a DNA sequence coding carboxyl terminal peptide (CTP) of human chorionic gonadotropin, (ii) a DNA fragment corresponding woodchuck hepatitis virus posttranscriptional regulatory element (WPRE). Evaluation of expression profile of the recombinant FSH using reverse transcription PCR and enzyme-linked immunosorbent assay (ELISA). Among three cell lines tested, HeLa cells were the best for hFSH expression (5,395 mIU/ml), then followed by chicken embryonic fibroblast (CEF) cells and Chinese hamster ovary (CHO) cells in the order of hFSH production. In addition to the amount, the FSH produced from HeLa cells was highest in terms of biological activity which was determined by measuring cAMP.

• Proceedings of the KWS Conference
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• 2006.10a
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• pp.63-65
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• 2006

The paper deals with the effect of hydrogen or helium in argon as a shielding gas on GTA welding of austenitic stainless steel. The studies were carried out in GTA(Gas Tungsten Arc) welding with a non-consumable electrode in case with different volume additions of hydrogen or helium to the argon shielding gas, i.e $5%H_2,\;10%H_2$, 30%He and 67%He. The penetration, welding voltage, microstructure and mechanical property were examined. The deepest penetration was obtained from the sample which was welded under shielding gas of $10%H_2$. The studies showed that hydrogen or helium addition to argon changes the static characteristic of the welding arc. The hydrogen or helium addition to argon increases arc power and the quantity of the material melted. The weld metal penetration depth and its width increased with increasing hydrogen or helium content. Additionally, welding voltage increased with increasing hydrogen or helium content.

• Journal of Sasang Constitutional Medicine
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• v.12 no.2
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• pp.67-77
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• 2000

1. Object of research Dongyi Soose Bowon Sasang Chobongyun and DongmuYugo were written by Lee je-ma befare Dongyi Soose Bowon. He explained in full detail Sasang Constitutional symtoms more than symtoms in Dongyi Soose Bowon. I tried to find his early medical views on Sasang Constitutional disease. 2. Method of research It was researched as contents with his writing surh as Dongyi Soose Bowon Sasang Chobongyun and DongmuYugo. 3. Results and conclusions (1) Dongmu was tried to explain the Sasang Constitutional physiological and pathological symtoms with ordinary symtoms. It is a his own implementation that he made an analysis of Sasang Constitutional symtoms. (2) We can take advantage of the contents clinically which has full of expression that there is a difference between the constitutional symtoms. (3) It is a guide post that can be expected the appearance of disease even if it were not in poor health

• (The)Study of the Eastern Classic
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• no.67
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• pp.327-344
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• 2017

Zhou Dunyi is regarded as a precursor of Li-Xue, which is called Neo-Confucianism. So, it could be say that Zhou Dunyi is a precursor of Song-Ming-Li-Xue. As is well known, Zhu xi is a synthesizer of Li-Xue in Song Dynasty, which is called Dao-Xue; Wang Yangming is a synthesizer of Li-Xue in Ming Dynasty, which is called Xin-Xue. Dao-Xue and Xin-Xue were engaged in an unyielding confrontation for many centuries in Asian areas. It is certain that Dao-Xue and Xin-Xue both succeed to the resources of Zhou Dunyi together, but it is not easy to confirm that the resources of Zhou Dunyi are common to both sides taking an unyielding confrontation into consideration. Usually, Zhou Dunyi is recognized as a Master of Dao-Xue rather than Xin-Xue. But in this paper, I try to give a question to this prevalent view, like this: Yang-Ming-Xin-Xue much absorbed and developed the Thought of Zhou Dunyi than Zhu-Xi-Dao-Xue did. In order to prove this assertion, I take two steps. The first is about Daotong, the second is about sincerity. In conclusion, while Wang Yangming build his own thought of moral philosophy, he fully absorbed Zhou Dunyi's thought of sincerity, and concreted his typical moral philosophy, which is Liangzhi-Xue. I could say that besides the viewpoint of Zhu xi, there is another viewpoint of Wang Yangming to understand the thought of Zhou Dunyi properly.

• Journal of Haehwa Medicine
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• v.9 no.1
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• pp.513-545
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• 2000

I. Introduction Bi(痺) means blocking. It can reach at the joints or muscles or whole body and make pains. Numbness and movement disorders. BiJeung can be devided into SilBi and HeoBi. In SilBi there are PungHanSeupBi, YeolBi and WanBi. In HeoBi, there are GiHyeolHeoBi, EumHeoBi and YangHeoBi. The common principle for the treatment of BiJeung is devision of the chronic stage and the acute stage. In the acute stage, BiJeung is usually cured easily but in the chronic stage, it is difficult. In the terminal stage, BiJeung can reach at the internal organs. BiJeung is one kind of symptoms making muscles, bones and jonts feel pain, numbness or edema. For example it can be gout or SLE etc. Many famous doctors studied medical science by their fathers or teachers. So the history of medical science is long. So I studied ${\ll}Bijeungjujip{\gg}$. II. Final Decision 1. BanSuMun(斑秀文) thought that BiJeung can be cured by blocking of blood stream. So he insisted that the important thing to cure BiJeung is to improve the blood stream. He usually used DangGuiSaYeokTang(當歸四逆湯), DangGuiJakYakSanHapORyeongSan, DoHong-SaMulTang(桃紅四物湯), SaMyoSanHapHeuiDongTang and HwangGiGyeJiOMulTang. 2. JangGeonBu(張健夫) focused on soothing muscles and improving blood seam. So he used many herbs like WiRyeongSeon(威靈仙), GangHwal(羌活), DokHwal(獨活), WooSeul(牛膝), etc. Especially he pasted wastes of the boiled herbs. 3. OSeongNong(吳聖農) introduced four rules to treat arthritis. So he usually used SeoGak-SanGaGam(犀角散加減), BoYanHwanOTang(補陽還五湯), ODuTang(烏頭湯), HwangGiGyeJiOMulTang. 4. GongJiSin thought disk hernia as one kind of BiJeung. And he said that Pung can hurt upper limbs and Seup can hurt lower limbs. He used to use GyeJiJakYakJiMoTang(桂枝芍藥知母湯). 5. LoJiJeong(路志正) introduced four principles to treat BiJeung. He used BangPungTang(防風湯), DaeJinGuTang) for PungBi(風痺), OPaeTang(烏貝湯) for HanBi(寒痺), YukGunJaTang(六君子湯) for SeupBi(濕痺) and SaMyoTang(四妙湯), SeonBiTang(宣痺湯), BaekHoGaGyeTang(白虎加桂湯) for YeolBi(熱痺). 6. GangChunHwa(姜春華) discussed herbs. He said SaengJiHwang(生地黃) is effective for PungSeupBi and WiRyungSun(威靈仙) is effective for the joints pain. He usually used SipJeonDaeBoTang(十全大補湯), DangGuiDaeBoTang(當歸大補湯), YoukGunJaTang(六君子湯) and YukMiJiHwanTang(六味地黃湯). 7. DongGeonHwa(董建華) said that the most important thing to treat BiJeung is how to use herbs. He usually used CheonO(川烏), MaHwang(麻黃) for HanBi, SeoGak(犀角) for YeolBi, BiHae) or JamSa(蠶沙) for SeupBi, SukJiHwang(熟地黃) or Vertebrae of Pigs for improving the function of kidney and liver, deer horn or DuChung(杜沖) for improving strength of body and HwangGi(黃?) or OGaPi(五加皮) for improving the function of heart. 8. YiSuSan(李壽山) devided BiJeung into two types(PungHanSeupBi, PungYeolSeupBi). And he used GyeJiJakYakJiMoTang(桂枝芍藥知母湯) for the treatment of gout. And he liked to use HwanGiGyeJiOMulTangHapSinGiHwan 枝五物湯合腎氣丸) for the treat ment of WanBi(頑痺). 9. AnDukHyeong(顔德馨) made YongMaJeongTongDan(龍馬定痛丹)-(MaJeonJa(馬錢子) 30g, JiJaChung 3g, JiRyong(地龍) 3g, JeonGal(全蝎) 3g, JuSa(朱砂) 0.3g) 10. JangBaekYou(張伯臾) devided BiJeung into YeolBi and HanBi. And he focused on improving blood stream. 11. JinMuO(陳茂梧) introduced anti-wind and dampness prescription(HoJangGeun(虎杖根) 15g, CheonChoGeun 15g, SangGiSaeng(桑寄生) 15g, JamSa(蠶絲) 15g, JeMaJeonJa(制馬錢子) 3g). 12. YiChongBo(李總甫) explained basic prescriptions to treat BiJeung. He used SinJeongChuBiEum(新定推痺陰) for HaengBi(行痺), SinJeongHwaBiSan(新定化痺散) for TongBi(痛痺), SinJeongGaeBiTang(新定開痺湯) for ChakBi(着痺), SinJeongCheongBiEum(新定淸痺飮) for SeupYeolBi(濕熱痺), SinRyeokTang(腎瀝湯) for PoBi(胞痺), ORyeongSan for BuBi(腑痺), OBiTang(五痺湯) for JangBi(臟痺), SinChakTang(腎着湯) for SingChakByeong(腎着病). 13. HwangJeonGeuk(黃傳克) used SaMu1SaDeungHapJe(四物四藤合制) for the treatment of a acute arthritis, PalJinHpPalDeungTang(八珍合八藤湯) or BuGyeJiHwangTangHapTaDeungTang(附桂地黃湯合四藤湯) for the chronic stage and ByeolGapJeungAekTongRakEum(鱉甲增液通絡飮) for EumHeo(陰虛) 14. GaYeo(柯與參) used HwalRakJiTongTang(活絡止痛湯) for shoulder ache, SoJongJinTongHwalRakTank(消腫鎭痛活絡湯) for YeolBi(熱痺), LiGwanJeolTang(利關節湯) for ChakBi(着痺), SinBiTang(腎痺湯) for SinBi(腎痺) and SamGyoBoSinHwan(三膠補腎丸) for back ache. 15. JangGilJin(蔣길塵) liked to use hot-character herbs and insects. And he used SeoGeunLipAnTang(舒筋立安湯) as basic prescription. 16. RyuJangGeol(留章杰) used GuMiGangHwalTang(九味羌活湯) and BangPungTang(防風湯) at the acute stage, ODuTang(烏頭湯) or GyeJiJakYakJiMoTang(桂枝芍藥知母湯) for HanBi of internal organs, YangHwaHaeEungTang(陽和解凝湯) for HanBi, DokHwalGiSaengTang(獨活寄生湯), EuiYiInTang(薏苡仁湯) for SeupBi, YukGunJaTang(六君子湯) for GiHeoBi(氣虛痺) and SeongYouTang(聖兪湯) for HyeolHeoBi(血虛痺). 17. YangYuHak(楊有鶴) liked to use SoGyeongHwalHyelTang(疏經活血湯) and he would rather use DoIn(桃仁), HongHwa(紅花), DangGui(當歸), CheonGung(川芎) than insects. 18. SaHongDo(史鴻濤) made RyuPungSeupTang(類風濕湯)-((HwangGi 200g, JinGu 20g, BangGi(防己) 15g, HongHwa(紅花) 15g, DoIn(桃仁) 15g, CheongPungDeung(靑風藤) 20g, JiRyong(地龍) 15g, GyeJi(桂枝) 15g, WoSeul(牛膝) 15g, CheonSanGap(穿山甲) 15g, BaekJi(白芷) 15g, BaekSeonPi(白鮮皮) 15g, GamCho(甘草) 15g).

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.3
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• pp.381-389
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• 2000

The Jindo is a Korean native dog, well-known for its hunting and guarding abilities. When he gives his devotion to one individual, he gives it whole-heartedly. He is not tempted easily and impetuous. The breed was not developed. but the dog retained their original qualities -loyal, alert, fearless, obedient, watchful, intelligent, energetic- to survive in the harsh environment of the Jindo island. The dog had been spread over the entire Korean peninsula from the time unknown, and the ones in the Jindo island, isolated until lately, survived and maintained their original characteristics. They are now spread over the entire Jindo County consisted of many islands, whence the breed name came. The Jindo comes in a variety of colors and color combinations, with the fawn and white colorings predominant. The dog is one of the Korean natural monuments, protected by law since early 1960s. The Jindo gained official approval by the Federation Cynologique Internationale as a hunting dog. Apart from the basic housetraining, the dog rarely gets training. Many people have attempted to preserve its pure bloodlines and original qualities. Today, there are a total of 10,356 Jindoes being raised over the entire Jindo County, and many more are kept elsewhere. A research into genetic characteristics of the Jindo is now going on, using the technique of isozyme electrophoresis. The Jindo Dog Breeding Management Center has been reinforced lately, and in addition to their routines, the Center is to work on the breeding of the Jindo. Efforts should be made in the future to produce stable, trustworthy Jindoes according to their proposed use and to modify their temperament in order to make it more widely acceptable as a pet and companion dog in the strangers home.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.312-320
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• 2021

In this study, we aimed to develop a feed additive that can promote the growth of stone flounder (Kareius bicoloratus). To this end, we fermented mealworm medium (5%, w/v) with lactic acid bacteria (LAB) with antibacterial function (HeTm_LpWpPa) and evaluated its efficacy as a feed additive. Fish (approximately 5-6 g) were reared in six completely recirculating tanks (100 fish/tank) for 6 weeks. The total length of fish with and without HeTm_LpWpPa supplementation was 8.26 and 8.42 cm and body weight was 19.71 and 20.28 g, respectively, at 6 weeks. The weight gain of the control and experimental groups was 223.26 % and 248.76 %, respectively. The specific growth rate at 6 weeks was 2.79 % (control) and 2.98% (experimental), and the growth rate of the experimental group was slightly higher (0.19 %) than that of the control group. At the end of the experiment (i.e. 6 weeks), the number of fish below the mean weight in the experimental group was 3 (1 %) and that in the control group was 17 (5.7 %). These results indicate that feed supplementation of 10 % HeTm_LpWpPa could be utilized to promote the growth of flounder in farming.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.2
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• pp.424-427
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• 2007

A fully differential transconductor using the series composite transistor is proposed. Simulation results show that THD is less than 1.2% for the differential input signal of up to $1.5V_{p-p}$ when the input signal frequency is 10MHz. i he proposed transconductor is used to design a third-order elliptic Gm-C lowpass filter with 138kHz cutoff frequency for ADSL Tx filter. The design procedure is based on signal flow graph(SFG) of a doubly-terminated LC ladder filter by means of fully differential transconductors and capacitors. The filter is fabricated and measured with a $0.35{\mu}m$ CMOS process.

• Proceedings of the KIEE Conference
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• 2000.07c
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• pp.1727-1729
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• 2000

We has investigated the birefringence by the assisted electric field effect on $As_{40}Ge_{10}Se_{15}S_{35}$ thin films. Photoinduced birefringence has been studied in a chalcogenide material. We induced this thin films using linearly polarized He-Ne laser light(633nm) and detected polarized semiconductor laser light(780nm). To investigate the effect of electric field, various bias voltages applied. The result is shown that the birefringence has a higher value in +2V than others. We obtained the birefringence in the electric field effects by various voltages.

• Journal of The Korean Astronomical Society
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• v.33 no.2
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• pp.97-110
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• 2000

Planetary nebulae provide a direct way to probe elemental abundances, their distributions and their gradients in populations in nearby galaxies. We investigate bulge planetary nebulae in M 31 and M 32 using the strong emission lines, H$\alpha$, He I, [O III], [N II], [S II] and [Ne III]. From the [O III] 4363/5007 line ratio and the [O II] 3727/3729, we determine the electron temperatures and number densities. With a standard modeling procedure (Hyung, 1994), we fit the line intensities and diagnostic temperatures, and as a result, we derive the chemical abundances of individual planetary nebulae in M 31 and M 32. The derived chemical abundances are compared with those of the well-known Galactic planetary nebulae or the Sun. The chemical abundances of M 32 appear to be less enhanced compared to the Galaxy or M 31.