• 한국미생물·생명공학회지
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• 제21권6호
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• pp.609-614
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• 1993

The optimum conditions for the submerged mycelial culture of Lentinus edodes SR-1 were elucidated to be incubation temperature of 25C, initial pH 4.0, agitation of 300 rpm, inoculation of 10.0%(v/v), and aeration of 1.0 v/v/m in TGY medium. The optimum c/n ratio and economic yield coeffcient for the submerged mycelial culture were 13.1:1 and 0.45 respectively. As the plant growth hormones test, SCM medium containing 0.5ppm of 2,4-dicholorophenoxyacetic acid increased mycelial yield in 1.1%, but 6-benzylaminopurine was not effective.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권5호
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• pp.341-346
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• 2001

For the purpose of mass producing Monascus red pigments optimum medium composition and environmental conditions were investigated in submerged flask cultures. The optimum carbon and nitrogen sources were determined to be 30g/L of glucose and 1.5 g/L of monosodium glutamate (MSG). Of the three metals examined, Fe$\^$2+/ showed the strongest stimulatory effect on pigment production and some stimulatory effect was also found in Mn$\^$2+/. Optimum pH and agitation speed were determined to be 6.5 and 700 rpm, respectively. Under the optimum culture conditions batch fermentation showed that the maximum biomass yield and specific productivity of red pigments were 0.20 g DCW/g glucose and, 32.5 OD$\sub$500/g DCW$\^$-1/h$\^$-1/, respectively.

• 한국균학회지
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• 제35권1호
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• pp.37-42
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• 2007

잣버섯 균사체 배양에 적합한 생물반응기는 풍선형의 공기부양식의 생물반응기가, 수용성 다당체의 생산에도 생장이 좋았던 풍선형 공기부양식 생물반응기가 유리한 것으로 나타났다. 배지의 탄소원 농도별에 따른 수용성 세포외 다당체의 생산량은 농도 처리간 차이가 없었으나 수용성 세포내 다당체는 탄소원 농도가 낮은 즉 C/N율이 낮을수록 증가하였다. 잣버섯 균사체는 고농도의 배지 배양조건에서 생물반응기내로 배지 공급형태가 배지량 증가방법보다 배지농도 조절에 의한 연속 배양방법이 우수한 것으로 나타났다.

• Mycobiology
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• 제37권2호
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• pp.89-93
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• 2009

The effect of fermentation parameters and medium composition on the simultaneous mycelial growth and exo-polymer production from submerged cultures of Ganoderma applanatum was investigated in shake-flask cultures. The optimum initial pH for mycelial growth and exo-polymer production was 5.0 and 6.0, respectively. The optimum temperature was $25^{\circ}C$ and the optimum inoculum content was 3.0% (v/v). The optimal carbon and nitrogen sources were glucose and corn steep powder, respectively. After 12 days fermentation under these conditions, the highest mycelial growth was 18.0 g/l and the highest exo-polymer production was 3.9 g/l.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1653-1659
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• 2015

Epothilone, which is produced by the myxobacterium Sorangium cellulosum, contributes significant value in medicinal development. However, under submerged culture conditions, S. cellulosum will accumulate to form bacterial clumps, which hinder nutrient and metabolite transportation. Therefore, the production of epothilone by liquid fermentation is limited. In this study, diatomite-based porous ceramics were made from diatomite, paraffin, and poremaking agent (saw dust). Appropriate methods to modify the porous ceramics were also identified. After optimizing the preparation and modification conditions, we determined the optimal prescription to prepare high-performance porous ceramics. The structure of porous ceramics can provide a solid surface area where S. cellulosum can grow and metabolize to prevent the formation of bacterial clumps. S. cellulosum cells that do not form clumps will change their erratic metabolic behavior under submerged culture conditions. As a result, the unstable production of epothilone by this strain can be changed in the fermentation process, and the purpose of increasing epothilone production can be achieved. After 8 days of fermentation under optimized conditions, the epothilone yield reached 90.2 mg/l, which was increased four times compared with the fermentation without porous ceramics.

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.784-788
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• 2000

Hypolipidemic effect of exo-polymers(EPs) form submerged mycelial culture of Cordyceps militaris was investigated in male Sprague-Dawley rats. For a dose-dependent study, EPs were administered at the level of 50-100 mg/kg body weight (BW) and the data was compared with the saline administered control group. A significant reduction of both the plasma total cholesterol (TC) and triglyceride (TG) was registered under the influence of EPs. It reduced the lowe density lipoprotein (LDL) cholesterol level as much as 40.5%. Levels of high density lipoprotein (HDL) choloesterol did not vary significantly within the different experimental groups, but the HDL: TC ratio showed consistently a high value with the increasing dose. The effects of cultural conditions (pH and temperature) in mycelial growth and EPs production were studied. Both the biomass and EPs were produced in a wide range of pH and temperature.

• Journal of Microbiology
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• 제44권2호
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• pp.233-242
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• 2006

The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM $MnCl_2$ at an initial pH 6.0 and temperature $31^{\circ}C$. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l) were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose medium. It may be worth attempting with other mushroom fermentation processes for enhanced production of mushroom polysaccharides, particularly those with industrial potential.

• Journal of Ginseng Research
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• 제24권2호
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• pp.58-63
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• 2000

식물조직배양기술을 이용하여 인삼 root를 생산하고자, 식물생장조절물질로 유도된 인삼 root를 사용하여 최적액체배양조건을 RSM을 이용하여 조사하였다. 최적액체배양조건을 배지의 pH, sucrose 농도, nitrogen 농도, phosphate 농도의 3 level-4factor의 fractional factorial design에 의하여 조사한 결과, 인삼 root의 생장율은 최저 1.00g에서 최고 2.33g까지 나타났다. 다중회기분석으로 구한 model식을 가지고 등고분석과 3차원분석을 수행한 후 독립변수의 최저 또는 최고수준에서 조속변수가 최대치를 나타내지 않는 배지의 pH와 sucrose농도의 변수에 대하여 model식을 편미분한 결과 인삼 root의 최적액체배양조건은 pH5.6, sucrose 3.8%, nitrogen 50mg/L, phosphate 80.7mg/L로 예측되었다. 이렇게 결정된최적조건값들을 model식에 대입하여 얻은 예상치는 2.36g 이었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제4권1호
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• pp.41-45
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• 1999

Microbial cellulose has many potential applications due to its excellent physical properties. The production of cellulose from Acetobacter xylinum in submerged culture is, however, beset with numerous problems. The most difficult one has been the appearance of negative mutants under shaking culture conditions, which is deficient of cellulose producing ability. Thus genetic instability of Acetobacter xylinum under shaking culture condition made developing a stable mutant major research interest in recent years. To find a proper type of bioreactor for the production of microbial cellulose, several production systems were developed. Using a reactor system with planar type impeller with bottoms sparging system, it was possible to produce 5 g/L microbial cellulose without generating cellulose minus mutants, which is comparable to that of static culture system.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2002년도 제35회 학술심포지움 및 춘계학술대회
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• pp.75-75
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• 2002