• Title/Summary/Keyword: Submanidbular gland

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Substrate Specificity of Mouse Glandular Kallikreins, Epidermal Growth Factor-Binding Protein Type A, B, and c against Mouse Ren 2 Prorenin (생쥐 선상칼리크레인(상피세포증식인자 결합단백질 Type A, B, 그리고 C)의 Ren 2 Prorenin에 대한 기질특이성)

  • 김화선;이희섭전병훈김원신
    • The Korean Journal of Zoology
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    • v.39 no.2
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    • pp.215-222
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    • 1996
  • In the previous studies, we have demonstrated that prorenin converting enzyme (PRECE) was identical to the epidermal grouch factor-binding protein (EGF-BP) type B, which was a member of the mouse glandular kallikrein family, To examine whether or not EGF-BP type A and C are involved in the processing of prorenin, we have cloned the CDNAS of the EGF-BP type h and C from a library of male ICR mouse submandibular gland (SMGI. And then CHO cells were transfected with the EGF-BP expression plasmids. and stable cell lines expressing a high level of the EGF-BPS precursor were obtained. The conditioned medium was then treated with trypsin, which has been knotvn to effectively convert the EGF-BP type A and C precursor to the active forms. 수ubsequentlv, the prorenin converting activity of the trypsin-treated or untreated medium was examined. PRECE converted exactly prorenin to renin, but the prorenin converting activities of EGF-BP type A and C were not detected. From these results, it seems that only type B of these EGF-BPs is involved in processing Ren 2 prorenin in mouse SMG.

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