• Journal of the Korean Magnetic Resonance Society
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• v.12 no.2
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• pp.65-73
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• 2008

HP1298 (Swiss-Prot ID ; P65108) is an 72-residue protein from Helicobacter pylori strain 26695. The function of HP1298 was identified as Translation initiation factor IF-l based on sequence homology, and HP1298 is included in IF-l family. Here, we report the sequence-specific backbone resonance assignments of HP1298. About 97% of all the $^{1}HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$, and $^{13}CO$ resonances could be assigned unambiguously. We could predict the secondary structure of HP1298, by analyzing the deviation of the $^{13}C{\alpha}$ and $^{13}C{\beta}$ shemical shifts from their respective random coil values. Secondary structure prediction shows that HP1298 consists of six $\beta$-strands. This study is a prerequisite for determining the solution structure of HP1298 and investigating the structure-function relationship of HP1298. Assigned chemical shift can be used for the study on interaction between HP1298 and other Helicobacter pylori proteins.

• Geomechanics and Engineering
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• v.17 no.6
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• pp.565-572
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• 2019

Spatial variability is an inherent characteristic of soil, and auto-correlation length (ACL) is a very important parameter in the reliability or probabilistic analyses of geotechnical engineering that consider the spatial variability of soils. Current methods for estimating the ACL need a large amount of laboratory or in-situ experiments, which is a great obstacle to the application of random field theory to geotechnical reliability analysis and design. To estimate the ACL reasonably and efficiently, we propose a micro-structure based numerical simulation method. The quartet structure generation set algorithm is used to generate stochastic numerical micro-structure of soils, and scanning electron microscope test of soil samples combined with digital image processing technique is adopted to obtain parameters needed in the QSGS algorithm. Then, 2-point correlation function is adopted to calculate the ACL based on the generated numerical micro-structure of soils. Results of a case study shows that the ACL can be estimated efficiently using the proposed method. Sensitivity analysis demonstrates that the ACL will become stable with the increase of mesh density and model size. A model size of $300{\times}300$ with a grid size of $1{\times}1$ is suitable for the calculation of the ACL of clayey soils.

• Journal of the Society of Naval Architects of Korea
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• v.43 no.3 s.147
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• pp.362-374
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• 2006

Currently, all design information of a hull structure is being first defined on 2D drawings not 3D CAD model at the initial ship design stage and then transferred to following design stages through the 2D drawings. This is caused by the past design practice, limitation on time, and lack of hull structural CAD systems supporting the initial design stage. As a result, the following design tasks such as the process planning and scheduling are being manually performed using the 2D drawings. For solving this problem, a data structure supporting the initial design stage is proposed and a prototype system is developed based on the data structure. The applicability of the system is demonstrated by applying it to various examples. The results show that the system can be effectively used for generating the 3D CAD model of the hull structure at the initial design stage.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.59-66
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• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• Journal of the Korean Society for Precision Engineering
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• v.19 no.10
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• pp.45-51
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• 2002

To achieve the lightweight and robust design of a structure, it is requested to design a structure and its control system simultaneously, which is called as the combined optimal design. A constant-cross-sectional area cantilever beam was chosen as an example for the applying the optimum design method. An initial load and a time varying disturbance were applied at the free end of the beam. Sliding mode control was selected due to its insensitiveness to the disturbance compared with other modes. It is known that the sliding mode control is robust to the disturbance and the uncertainty only if a matching condition is met, after giving a switching hyper plane. In this study, the optimum method was used for the design of the switching hyper plane and the objective function of the optimum switching hyper plane was assumed to be the objective one of the control system. The total weight of the structure was treated as a constraint and the cross sectional areas of the beam were considered as design variables, which means a nonlinear programming problem. The sequential linear programming method was applied to solve it. As a result of the optimum design, the effect of attenuating vibrations has been improved obviously. Moreover, lightweight design of the structure became possible from the relationship of the weight of the structure and the control objective function.

• Journal of the Society of Naval Architects of Korea
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• v.49 no.2
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• pp.158-163
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• 2012

Recently, reducing underwater radiated noise (URN) of ships has become an environmental issue to protect marine wildlife. URN of ships can be predicted by various methods considering its generating mechanism and frequency ranges. For URN prediction due to ship structural vibration in low frequency range, the fluid-structure interaction analysis technique based on finite element and boundary element methods (FE/BEM) is regarded as an useful technique. In this paper, URN due to a double bottom-shaped structure vibration has been numerically investigated based on a coupled method of FE/BEM to enhance the prediction accuracy of URN due to the vibration of real ship engine room structure. Acoustic radiation efficiency and URN transfer function in case of vertical harmonic excitation on the top plate of double bottom structure have been evaluated. Using the results, the validity of an existing empirical formula for acoustic radiation efficiency estimation and a simple URN transfer function, which are usually adopted for URN assessment in initial design stage, is discussed.

• BMB Reports
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• v.33 no.3
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• pp.195-201
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• 2000

A human gene has been reported that may encode the enzyme acetohydroxyacid synthase. Previously this enzyme was thought to be absent from animals although it is present in plants and many microorganisms. In plants, this enzyme is the target of a number of commercial herbicides and the use of these compounds may need to be reassessed if the human enzyme exists and proves to be susceptible to inhibition. Here we report the construction of several plasmid vectors containing the cDNA sequence for this protein, and their expression in Escherichia coli. High levels of expression were observed, but most of the protein proved to be insoluble. The small amounts of soluble protein contained little or no acetohydroxyacid synthase activity. Attempts to refold the insoluble protein were successful insofar as the protein became soluble. However, the refolded protein did not gain any acetohydroxyacid synthase activity. In vivo complementation tests of an E. coli mutant produced no evidence that the protein is active. Incorrect folding, or the lack of another subunit, may explain the data but we favor the interpretation that this gene does not encode an acetohydroxyacid synthase.

• Transactions of the Korean Society of Automotive Engineers
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• v.15 no.3
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• pp.63-71
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• 2007

During the design phase of a product, reliability and design engineers are called upon to evaluate the reliability of the system, The question of how to meet target reliability for the system arises when estimated reliability or cost is inadequate. This then becomes a problem of reliability allocation and system structure design. This study proposes the optimization methodology to achieve target reliability with minimum cost through construction of the cost function of system. In cost function, total cost means the sum of initial cost, repair cost and maintenance cost. This study constructs optimization problem about system structure design and reliability allocation using cost function. This problem constructed is solved by Multi-island Genetic Algorithm(MIGA), and applies to urban transit brake system. Current brake system of the urban transit is series system. Series system is the simplest and perhaps one of the most common system, but it demands high reliability and maintenance cost because all components must be operating to ensure system operation. Thus this study makes a comparative study by applying k-out-of-n system to brake system. This methodology presented can be a great tool for aiding reliability and design engineers in their decision-makings.

• Lingua Humanitatis
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• v.8
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• pp.369-387
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• 2006

Which syntactic function should we assign to the 'ga-type' constituent which occurs in the morphological passive constructions in Korean, [N0-neun N1-i Vpass-ending]? This problem is very important in two respects. First, a small change of status of the particle 'i/ga' can exert an overall influence on the Korean grammar. Second, the particle '-i/ga' cannot guarantee that 'ga-type' constituents are subject of the sentence, so that the concept of syntactic category should be distinguished from that of syntactic function. This paper claims that the analysis of sentence has long been focused on the structure of proposition, namely the argument structure and that the direction of analysis should be turned to the 'person structure' which can be revealed on the pragmatic level. On the basis of this, this paper suggests that the specific type of the morphological passive constructions in Korean, [N0-neun N1-i Vpass-ending] should be analysed in line with the psych-verb constructions and that the modal meaning 'potential' of the passive constructions is correlated with sentence pattern and 'person structure'.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.12
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• pp.930-937
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• 2003

This research deals with how to select connection positions of two substructures to be synthesized. The goal of this research is to find optimal connection positions in order to maximize the fundamental natural frequency of the synthesized structure. The natural frequencies of a connected structure are obtained by modal-force equations. Optimal connection positions can be selected through optimization process. In the optimization process, the natural frequencies of a connected structure are set to object function value and connection positions become design variables. The method described above is applied to synthesis problems of plates, which is initially conducted for FE models and verified through experiments. Especially in experiments. FRF(frequency response function) s are obtained by means of the Modal Testing technique to be used in modal-force equations for synthesizing. Once the substructures are synthesized. the Modal Testing technique is again applied to spot-welded structure using the result from the optimization procedure. It is found that the fundamental natural frequency of the synthesized structure with the optimized result gives higher value than those with the initially given connection positions.