• Title/Summary/Keyword: Structural response analysis

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Proline Accumulation and P5CS ($\Delta^1$-pyrroline-5-carboxylate synthetase) Gene Expression in Response to Salt Stress in Zoysiagrasses

  • Lee, Dong-Joon;Hwang, Cheol-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.1
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    • pp.20-24
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    • 2003
  • Proline is known as an osmotrotectant to enhance tolerance against both salt and dehydration stresses. A P5CS ($\Delta^1$-pyrroline-5-carboxylate synthetase) plays a major role in regulation of synthesis of proline. An overexpression of the mothbean P5CS gene in transgenic tobacco plant increased the levels of proline and osmotolerance. In an attempt to look for the possibility to use content of proline as well as a level of P5CS gene expression as molecular markers for salt tolerance, the amounts of proline and transcript levels of P5CS were measured as functions of either concentration of NaCl or length of treatment period among different species of zoysiagrass. Hybridzoysia showed the highest level of proline ($329\mu\textrm{g}$/g.f.w.) among five different species of zoysiagrass at 250 mM NaCl in 24 hours. The level of P5CS transcript was also the highest in the hybridzoysia at 250 mM NaCl in 24 hours. The transcriptions of P5CS gene were induced at the rates of 1.2, 1.2, 1.8, and 1.8, upon treatment of 250 mM NaCl in Z. japonica, Z. matrella, Z. sinica and hybridzoysia respectively. Based on a correlation between the level of P5CS transcript and the proline content among different species of zoysiagrass, a comparative structural analysis of the gene for P5CS from either Z. sinica or hybridzoysia may lead to an understanding of mechanism for salt tolerance shown differently among zoysiagrasses.

Parametric Study of MD Constitutive Model for Coarse-Grained Soils (조립재료에 대한 MD구성모델의 매개 변수 연구)

  • Choi, Changho
    • Journal of the Korean Geosynthetics Society
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    • v.12 no.1
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    • pp.11-19
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    • 2013
  • Coarse-grained soils are typical engineering materials commonly used in many civil engineering applications such as structural fills, subgrade and drainage fills for dam, railway and bridge. Various researches have been performed with related to constitutive laws for numerical analysis of such structures. This paper presents a parametric study for a constitutive model for coarse grained materials. The model is a kind of the bounding surface models based on critical state theory. A distinct feature of the model is to capture the response of coarse-grained materials with different void ratios and confining pressures using a single set of model parameters. The model behavior is defined with a set of elastic parameters, critical state parameters, and model-specific parameters. The parametric study was performed for the model-specific parameters. The result of parametric study shows that the model is capable to capture stress-dilatancy behavior and kinematic-hardening under non-associative plastic flow.

Gas Sensing Characteristics of Nano Sized SnO2 Sensors for Various Co and Ni Concentration (Co, Ni 농도 변화에 따른 나노 SnO2 센서의 감응 특성)

  • Lee, Ji-Young;Yu, Yoon-Sic;Yu, Il
    • Korean Journal of Materials Research
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    • v.21 no.10
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    • pp.546-549
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    • 2011
  • Nano-sized $SnO_2$ thick films were prepared by a screen-printing method onto $Al_2O_3$ substrates. The sensing characteristics were investigated by measuring the electrical resistance of each sensor in a test box as a function of the detection gas. The nano-sized $SnO_2$ thick film sensors were treated in a $N_2$ atmosphere. The structural properties of the nano $SnO_2$with a rutile structure according to XRD showed a (110) dominant $SnO_2$ peak. The particle size of $SnO_2$:Ni nano powders at Ni 8 wt% was about 45 nm, and the $SnO_2$ particles were found to contain many pores according to the SEM analysis. The sensitivity of the nano $SnO_2$-based sensors was measured for 5 ppm $CH_4$ gas and $CH_3CH_2CH_3$ gas at room temperature by comparing the resistance in air with that in the target gases. The results showed that the best sensitivity of $SnO_2$:Ni and $SnO_2$:Co sensors for $CH_4$ gas and $CH_3CH_2CH_3$ gas at room temperature was observed in $SnO_2$:Ni sensors doped with 8 wt% Ni. The response time of the $SnO_2$:Ni gas sensors was 10 seconds and recovery time was 15 seconds for the $CH_4$ and $CH_3CH_2CH_3$ gases.

Study on Oil Production from Pyrolysis of Mixed Plastic Waste Using Multidimensional Chromatography (Multidimensional Chromatography/Mass Spectrometry를 이용한 혼합 폐플라스틱의 열분해 오일 특성 평가에 관한 연구)

  • 김석완
    • Journal of Environmental Science International
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    • v.11 no.4
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    • pp.375-382
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    • 2002
  • The total hydrocarbon distribution of oil products obtained from the pyrolysis of four kinds of mixtures of polyethylene-polystyrene waste has been studied by multidimensional chromatography(high performance liquid chromatography followed by capillary gas chromatography)/mass spectrometry. Saturated, unsaturated and aromatic hydrocarbons in oil products were selectively pre-separated according to structural groups by HPLC and the weight fraction of each group was estimated by analysis of each component using GC-FID response factors. The hydrocarbon distribution of aliphatic fraction consists of $C_{5}$ to $C_{25}$ saturated and unsaturated hydrocarbons. And that of aromatics fraction consists of benzene, toluene, xylene, styrene, propenyl benzene, naphthalene, and some of derivatives. Pyrolysis temperature did not affect the ratio of total weight fraction of aliphatic over aromatic hydrocarbon distribution in case of PS only and PE-PS mixtures (1:1 and 1:4 wt. ratio) as a feed while affected the ratio of total wt. fraction in case of PE only. The optimal temperature for the maximum oil production was $600^{\circ}C$ for pyrolysis of PS and 1:1 and 1:4 mixtures of PE and PS. The optimal condition for aromatic recovery was $600^{\circ}C$ with 1:1 mixture of PE and PS. In this condition, aromatic was produced up to 90% of total oil product. The maximum yield of toluene, xylene, styrene, and propenyl benzene were 8.6, 8.9, 51.0 and 7.4% of feed for pyrolysis PS at $700^{\circ}C$, respectively. However, only 1.3% naphthalene was recovered at $700^{\circ}C$ with 1:1 PE:PS(by wt.).

A "Dynamic Form-Finding" Approach to Environmental-Performance Building Design

  • Yao, Jia-Wei;Lin, Yu-Qiong;Zheng, Jing-Yun;Yuan, Philip F.
    • International Journal of High-Rise Buildings
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    • v.7 no.2
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    • pp.145-151
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    • 2018
  • Newly-designed high-rise buildings, both in China and abroad, have demonstrated new innovations from the creative concept to the creative method. from the creative concept to the creative method. At the same time, digital technology has enabled more design freedom in the vertical dimension. "Twisting" has gradually become the morphological choice of many city landmark buildings in recent years. The form seems more likely to be driven by the interaction of aesthetics and structural engineering. Environmental performance is often a secondary consideration; it is typically not simulated until the evaluation phase. Based on the research results of "DigitalFUTURE Shanghai 2017 Workshop - Wind Tunnel Visualization", an approach that can be employed by architects to design environmental-performance buildings during the early stages has been explored. The integration of a dynamic form-finding approach (DFFA) and programming transforms the complex relationship between architecture and environment into a dialogue of computer language and dynamic models. It allows the design to focus on the relationship between morphology and the surrounding environment, and is not limited to the envelope form itself. This new concept of DFFA in this research consists of three elements: 1) architectural form; 2) integration of wind tunnel and dynamic models; and 3) environmental response. The concept of wind tunnel testing integrated with a dynamic model fundamentally abandons the functional definition of the traditional static environment simulation analysis. Instead it is driven by integral environmental performance as the basic starting point of morphological generation.

Analysis of cutting forces and roughness during hard turning of bearing steel

  • Bouziane, Abderrahim;Boulanouar, Lakhdar;Azizi, Mohamed Walid;Keblouti, Ouahid;Belhadi, Salim
    • Structural Engineering and Mechanics
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    • v.66 no.3
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    • pp.285-294
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    • 2018
  • An experimental study has been carried out to analyze the effect of cutting parameters (cutting speed, feed and depth of cut) and tool nose radius on the surface roughness and the cutting force components during hard turning of the AISI 52100 (50 HRC) steel with a ceramic cutting tool. The tests have been conducted according to the methodology of planning experiments, based on an orthogonal plan of Taguchi (L27). By using the response surface methodology (RSM), the components of the cutting force and the roughness of the machined surface were modeled and the effects of the input parameters were analyzed statistically by ANOVA and RSM. The results show that the feed (f), the tool nose radius (r), the cutting speed (Vc), the interaction between feed and tool nose radius ($f{\times}r$) as well as that of the quadratic effect ($f^2$) all have significant effects on the surface roughness (Ra). The feed is the most influencing factor with a contribution of 47.31%. The components of the cutting force were strongly influenced by the depth of cut, followed by the advance with a lower degree. By comparing the experimental values with those predicted by the models of the cutting force components and the surface roughness, it appears that they are in very good correlation.

Free vibration of FGM plates with porosity by a shear deformation theory with four variables

  • Yousfi, Mahfoud;Atmane, Hassen Ait;Meradjah, Mustapha;Tounsi, Abdelouahed;Bennai, Riadh
    • Structural Engineering and Mechanics
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    • v.66 no.3
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    • pp.353-368
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    • 2018
  • In this work, a high order hyperbolic shear deformation theory with four variables is presented to study the vibratory behavior of functionally graduated plates. The field of displacement of the theory used in this work is introduced indeterminate integral variables. In addition, the effect of porosity is studied. It is assumed that the material characteristics of the porous FGM plate, varies continuously in the direction of thickness as a function of the power law model in terms of volume fractions of constituents taken into account the homogeneous distribution of porosity. The equations of motion are obtained using the principle of virtual work. An analytical solution of the Navier type for free vibration analysis is obtained for a FGM plate for simply supported boundary conditions. A comparison of the results obtained with those of the literature is made to verify the accuracy and efficiency of the present theory. It can be concluded from his results that the current theory is not only accurate but also simple for the presentation of the response of free vibration and the effect of porosity on the latter.

Pavement condition assessment through jointly estimated road roughness and vehicle parameters

  • Shereena, O.A.;Rao, B.N.
    • Structural Monitoring and Maintenance
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    • v.6 no.4
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    • pp.317-346
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    • 2019
  • Performance assessment of pavements proves useful, in terms of handling the ride quality, controlling the travel time of vehicles and adequate maintenance of pavements. Roughness profiles provide a good measure of the deteriorating condition of the pavement. For the accurate estimates of pavement roughness from dynamic vehicle responses, vehicle parameters should be known accurately. Information on vehicle parameters is uncertain, due to the wear and tear over time. Hence, condition monitoring of pavement requires the identification of pavement roughness along with vehicle parameters. The present study proposes a scheme which estimates the roughness profile of the pavement with the use of accurate estimates of vehicle parameters computed in parallel. Pavement model used in this study is a two-layer Euler-Bernoulli beam resting on a nonlinear Pasternak foundation. The asphalt topping of the pavement in the top layer is modeled as viscoelastic, and the base course bottom layer is modeled as elastic. The viscoelastic response of the top layer is modeled with the help of the Burgers model. The vehicle model considered in this study is a half car model, fitted with accelerometers at specified points. The identification of the coupled system of vehicle-pavement interaction employs a coupled scheme of an unbiased minimum variance estimator and an optimization scheme. The partitioning of observed noisy quantities to be used in the two schemes is investigated in detail before the analysis. The unbiased minimum variance estimator (MVE) make use of a linear state-space formulation including roughness, to overcome the linearization difficulties as in conventional nonlinear filters. MVE gives estimates for the unknown input and fed into the optimization scheme to yield estimates of vehicle parameters. The issue of ill-posedness of the problem is dealt with by introducing a regularization equivalent term in the objective function, specifically where a large number of parameters are to be estimated. Effect of different objective functions is also studied. The outcome of this research is an overall measure of pavement condition.

Optimal Conditions for the Expression of Glycoprotein E2 of Classical Swine Fever Virus using Baculovirus in Insect Cells

  • Bae, Sung Min;Lee, Seung Hee;Kwak, Won Suk;Ahn, Yong Oh;Shin, Tae Young;Woo, Soo Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.29 no.2
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    • pp.207-213
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    • 2014
  • The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

Study for the Development of a Main Oxidizer Shut-off Valve for Liquid Rocket Engines (발사체 연소기용 산화제 개폐밸브의 핵심요소 기술 개발)

  • Kim, Dohyung;Hong, Moongeun;Park, Jaesung;Lee, Soo Yong
    • Journal of the Korean Society of Propulsion Engineers
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    • v.17 no.6
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    • pp.113-119
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    • 2013
  • A main oxidizer shut-off valve (MOV) controls the supply of cryogenic liquid oxygen to the combustion chamber of liquid rocket engines by on/off operations. The main subjects to be introduced are not only the valve transient response during valve on/off procedures but also the characteristics of pneumatic and seat/poppet parts as core technologies in the development of the MOV, which is expected to be adopted for the Korea Space Launch Vehicle II. It is shown that the analytical prediction of the transient valve travel is in good agreement with experimental results. Friction and elastic forces on the valve moving part are quantitatively evaluated by structural analysis.