• 생물환경조절학회지
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• 제19권2호
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• pp.63-69
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• 2010

2007년 4월 이전까지 10~15년 동안 규격시설로 운영되어 오던 농가지도형 단동하우스에서의 보강지주 설치효과를 분석하였다. 이전의 연구로 적설하중에 대한 수식 계산을 통해 보강지주 설치 단동하우스의 추가적설심이 제시된 바 있으나 모델이 농가지도형 단동하우스 규격과 상이해 연구 결과를 농가지도형 단동하우스에 그대로 적용하기에 무리가 따랐다. 본 연구에서는 농가지도형 단동하우스를 3차원 강뼈대 구조물로 모델링 하여 보강지주 설치 효과를 분석하였으며 재하시험을 통해 해석결과를 검증하였다. 구조해석 결과, 단동하우스에 보강지주 설치 시 안전적설심은 오히려 줄어드는 것으로 나타났는데 파이프 체결 부에서의 집중 하중으로 지붕도리에 큰 응력이 걸리기 때문으로 보강지주 설치 시에는 지붕도리의 규격도 함께 강화되어야 할 것으로 판단되었다. 지붕도리를 강화하고 보강지주를 3~4m 간격으로 설치할 경우, 안전적설심은 기본모델에서 보다 2배 이상 높아지는 것으로 나타났다. 보강지주 규격별 농가지도형 단동하우스 5종의 안전적설심을 제시하였다.

• 한국정보전자통신기술학회논문지
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• 제13권6호
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• pp.556-561
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• 2020

현대 사회의 농업 인구의 고령화 및 감소로 농업 환경 개선이 필요한 실정이고 대표적인 문제 중 하나이다. 그리고 대부분의 작업체계에서 항상 운반 작업이 필요하기 때문에 운반 작업에 사용되는 시간과 노동력의 비율이 매우 높다. 이에 따라 많은 종류의 운반차들이 개발되고 판매되고 있으며, 초기에는 대부분 화석연료를 사용하는 동력운반차가 주종을 이루고 있다. 그러나 최근에는 지구온난화와 기후변화협약 등 국제 환경규제의 강화 및 화석연료의 고갈로 인한 수소, 연료전지, 태양광, 바이오 등 차세대 친환경에너지를 주목하고 있다. 그래서 본 연구에서는 화석연료를 대체하는 친환경적이며 누구나 쉽게 조작이 가능하고 안전한 농업용 전기운반차를 개발하는 것을 최종목표로 한다. 농업용 전기운반차의 광범위한 차속 조절 및 안정성 확보에 초점을 두고 설계하였으며, 성능 및 디자인을 고려하여 프레임, 주행부, 조향부, 컨트롤러 시스템 등으로 구성되어 각 부분별 검토하였다. 본 연구의 농업용 전기운반차가 젊은 인력이 부족한 농경사회에서 고령노동자나 여성들이 쉽고 편하게 작업을 할 수 있고 높은 효율성을 통해서 농경사회에 도움이 될 수 있을 것이다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2002년도 학술발표대회
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2007년도 춘계학술대회 논문집
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• pp.379-382
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• 2007

The MEMS (Micro Electro Mechanical Systems) process is used in a micro/nano pattern manufacturing method. This method is based on the lithography technology. But the MEMS process has some problems such as complicated process, long processing time and high production costs. Many researchers are doing research in substitute manufacturing method to work out a solution to these problems. In this paper, we apply a dieless incremental forming technology to a substitute method of MEMS process. This dieless forming technology is using in the commercial scale sheet forming such as a prototype of automobile sheet parts. 5-axes CNC (Computerized Numeric Control) method are applied in this system to get a micro-scale dieless forming results. These 5-axes system are composed of precision AC servo motor stages (4-axes) and PZT actuator (1-axis). A PZT actuator is used in a precision actuating axis because it can be operated in the nano scale stroke resolution. This micro dieless incremental forming system has the advantage of minimization in manipulating distance and working space. As equipment and tools become smaller in size, minute inertia force and high natural frequency can be obtained. Therefore, high precision forming performance can be obtained. This allows the factory to quickly provide the customer with goods because the manufacturing system and process are reduced. To construct this micro manufacturing system, many technologies are necessary such as high stiffness frame, high precision actuating part, structural analysis, high precision tools and system control. To achieve the optimal forming quality, the micro dieless forming system is designed and made with high stiffness characteristic.

• Earthquakes and Structures
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• 제8권5호
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• pp.1215-1240
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• 2015

In this paper, the Theory of Plastic Mechanism Control (TPMC) is applied to the seismic design of dual systems composed by moment-resisting frames and Chevron braced frames. The application of TPMC is aimed at the design of dual systems able to guarantee, under seismic horizontal forces, the development of a collapse mechanism of global type. This design goal is of primary importance in seismic design of structures, because partial failure modes and soft-storey mechanisms have to be absolutely prevented due to the worsening of the energy dissipation capacity of structures and the resulting increase of the probability of failure during severe ground motions. With reference to the examined structural typology, diagonal and beam sections are assumed to be known quantities, because they are, respectively, designed to withstand the whole seismic actions and to withstand vertical loads and the net downward force resulting from the unbalanced axial forces acting in the diagonals. Conversely column sections are designed to assure the yielding of all the beam ends of moment-frames and the yielding and the buckling of tensile and compressed diagonals of the V-Braced part, respectively. In this work, a detailed designed example dealing with the application of TPMC to moment frame-chevron brace dual systems is provided with reference to an eight storey scheme and the design procedure is validated by means of non-linear static analyses aimed to check the actual pattern of yielding. The results of push-over analyses are compared with those obtained for the dual system designed according to Eurocode 8 provisions.

• Earthquakes and Structures
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• 제13권3호
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• pp.267-277
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• 2017

Base-isolation is now being adopted as a retrofitting strategy to improve seismic behaviour of reinforced concrete (r.c.) framed structures subjected to far-fault earthquakes. However, the increase in deformability of a base-isolated framed building may lead to amplification in the structural response under the long-duration horizontal pulses of high-magnitude near-fault earthquakes, which can become critical once the strength level of a fire-weakened r.c. superstructure is reduced. The aim of the present work is to investigate the nonlinear seismic response of fire-damaged r.c. framed structures retrofitted by base-isolation. For this purpose, a five-storey r.c. framed building primarily designed (as fixed-base) in compliance with a former Italian seismic code for a medium-risk zone, is to be retrofitted by the insertion of elastomeric bearings to meet the requirements of the current Italian code in a high-risk seismic zone. The nonlinear seismic response of the original (fixed-base) and retrofitted (base-isolated) test structures in a no fire situation are compared with those in the event of fire in the superstructure, where parametric temperature-time curves are defined at the first level, the first two and the upper levels. A lumped plasticity model describes the inelastic behaviour of the fire-damaged r.c. frame members, while a nonlinear force-displacement law is adopted for the elastomeric bearings. The average root-mean-square deviation of the observed spectrum from the target design spectrum together with a suitable intensity measure are chosen to select and scale near- and far-fault earthquakes on the basis of the design hypotheses adopted.

• Structural Engineering and Mechanics
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• 제39권1호
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• pp.125-145
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• 2011

Pushover analysis has gained significant popularity as an analytical tool for realistic determination of the inelastic behaviour of RC structures. Though significant work has been done to evaluate the demands realistically, the evaluation of capacity and realistic failure modes has taken a back seat. In order to throw light on the inelastic behaviour and capacity evaluation for the RC framed structures, a 3D Reinforced concrete frame structure was tested under monotonically increasing lateral pushover loads, in a parabolic pattern, till failure. The structure consisted of three storeys and had 2 bays along the two orthogonal directions. The structure was gradually pushed in small increments of load and the corresponding displacements were monitored continuously, leading to a pushover curve for the structure as a result of the test along with other relevant information such as strains on reinforcement bars at critical locations, failure modes etc. The major failure modes were observed as flexural failure of beams and columns, torsional failure of transverse beams and joint shear failure. The analysis of the structure was by considering all these failure modes. In order to have a comparison, the analysis was performed as three different cases. In one case, only the flexural hinges were modelled for critical locations in beams and columns; in second the torsional hinges for transverse beams were included in the analysis and in the third case, joint shear hinges were also included in the analysis. It is shown that modelling and capturing all the failure modes is practically possible and such an analysis can provide the realistic insight into the behaviour of the structure.

• Steel and Composite Structures
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• 제29권5호
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• pp.603-622
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• 2018

Precast concrete structures are erected from individual prefabricated components, which are assembled on-site using different types of connections. In the present design of these structures, beam-to-column connections are assumed pin jointed. Bolted billet beam to-column connections have been used in the precast concrete industry for many years. They have many advantages over other jointing methods in component production, quality control, transportation and assembly. However, there is currently limited information concerning their detailed structural behaviour under vertical loadings. The experimental work has involved the determination of moment-relative rotation relationships for semi-rigid precast concrete connections in full-scale connection tests. The study reported in this paper was undertaken to clarify the behaviour of such connections under symmetrical vertical loadings. A series of full-scale tests was performed on sample column for which the column geometry and bolt arrangements conformed to successful commercial practice. Proprietary hollow core floor slabs were tied to the beams by 2T25 tensile reinforcing bars, which also provide the in-plane continuity across the connections. The contribution of the floor strength and stiffness to the flexural capacity of the joint is currently neglected in the design process for precast concrete frames. The flexural strength of the connections in the double-sided tests was at least 0.93 times the predicted moment of resistance of the composite beam and slab. The secant stiffness of the connections ranged from 0.94 to 1.94 times the flexural stiffness of the attached beam. In general, the double-sided connections were found to be more suited to a semi-rigid design approach than the single sided ones. The behaviour of double sided bolted billet connection test results are presented in this paper. The behaviour of single sided bolted billet connection test results is the subject of another paper.

• Advances in nano research
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• 제14권5호
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• pp.435-442
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• 2023

Sports activities, including playing tennis, are popular with many people. As this industry has become more professionalized, investors and those involved in sports are sure to pay attention to any tool that improves athletes' performance Tennis requires perfect coordination between hands, eyes, and the whole body. Consequently, to perform long-term sports, athletes must have enough muscle strength, flexibility, and endurance. Tennis rackets with new frames were manufactured because tennis players' performance depends on their rackets. These rackets are distinguished by their lighter weight. Composite rackets are available in many types, most of which are made from the latest composite materials. During physical exercise with a tennis racket, nanocomposite materials have a significant effect on reducing injuries. Materials as strong as graphite and thermoplastic can be used to produce these composites that include both fiber and filament. Polyamide is a thermoplastic typically used in composites as a matrix. In today's manufacturing process, materials are made more flexible, structurally more vital, and lighter. This paper discusses the production, testing, and structural analysis of a new polyamide/Multi-walled carbon nanotube nanocomposite. This polyamide can be a suitable substitute for other composite materials in the tennis racket frame. By compression polymerization, polyamide was synthesized. The functionalization of Multi-walled carbon nanotube (MWCNT) was achieved using sulfuric acid and nitric acid, followed by ultrasonic preparation of nanocomposite materials with weight percentages of 5, 10, and 15. Fourier transform infrared (FTIR) and Nuclear magnetic resonance (NMR) confirmed a synthesized nanocomposite structure. Nanocomposites were tested for thermal resistance using the simultaneous thermal analysis (DTA-TG) method. scanning electron microscopy (SEM) analysis was used to determine pores' size, structure, and surface area. An X-ray diffraction analysis (XRD) analysis was used to determine their amorphous nature.

• Journal of the Korean Wood Science and Technology
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• 제41권3호
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• pp.234-246
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• 2013

목조주택의 골조 및 실내 외 마감용으로 사용되는 침엽수 구조재를 대상으로 방염 방충 보존처리제가 혼합된 약제에 마이크로파 가열 후 열기를 지닌체 침지하여 복합적인 기능화를 부여한 후 목재의 건조 스케줄, 방염 시험에 의한 화재 저항성과 흰개미에 의한 내충해성, 혼합 약제의 침투성 분석을 실시한 결과 마이크로파로 3 kW로 5분 급속 가열된 시험편은 목표 온도 및 함수율을 만족하는 것으로 나타났으며, 인산염과 헤테로고리화합물계가 혼합된 약제에 120분 침지할 경우 가장 높은 중량 증가율을 가지는 것으로 나타났다. 또한 방염 후 처리 물품 시험을 실시한 결과 인산염과 혼합된 약제는 방염기준을 모두 만족하는 것으로 나타났으며, 흰개미 투입 후 7일 사충율을 확인한 결과 인산염과 헤테로고리화합물계 혼합 약제 침투 시험편의 경우 96% 이상 높은 사충율을 나타냄으로서 가장 우수한 특성을 가지는 것으로 나타났다. 목재 내부로 약제 침투성 분석을 실시한 결과 목재 세포내부 전면에 있어 혼합약제가 침투된 것으로 나타나 목재의 방염성 및 방충 저항성 등에서 우수한 성능의 발현은 혼합 약제의 균일한 침투때문으로 판단된다.