• Archives of Plastic Surgery
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• 제35권3호
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• pp.248-254
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• 2008

Purpose: Human acellular dermal matrix(ADM) is widely used in the treatment of congenital anomalies and soft tissue deficiencies. But it is rapidly degraded in the body and does not provide satisfactory results. There is a need to improve collagen fiber stability through various methods and ultimately regulate the speed of degradation. Methods: The ADMs were added with various cross-linking agents called glutaraldehyde, dimethyl 3,3'-dithiobispropionimidate to produce cross-linked acellular dermal matrices. 1,4-butanediol diglycidyl ether solution was applied with a pH of 4.5 and 9.0, respectively. The stability of cross-linked dermal matrix was observed by measuring the shrinkage temperature and the degradation rates. The cross- and non-cross linked dermis were placed in the rat abdomen and obtained after 8, 12 and 16 weeks. Results: The shrinkage temperature significantly increased and the degradation rate significantly decreased, compared to the control(p<0.05). All of cross-linked dermises were observed grossly in 16 weeks, but most of non-cross linked dermis were absorbed in 8 weeks. Histologically, the control group ADM was found to have been infiltrated with fibroblasts and most of dermal stroma were transformed into the host collagen fibers. However, infiltration of fibroblasts in the experiment was insignificant and the original collagen structure was intact. Conclusion: Collagen cross-linking increases the structural stability and decreases degradation of acellular dermis. Therefore, decrease in body absorption and increase in duration can be expected.

• Biomolecules & Therapeutics
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• 제5권2호
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• pp.133-149
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• 1997

4-week repeated dose toxicity of DA-5018, a new capsaicin analogue analgesic agent, was examined in 5D rats at dosage levels of 0,0.4,2, 10 and 50 mg/kg/day. DA-5018 was administered orally to 17 males and 17 females per group at doses of 0, 10 and 50 mg/kg and to 12 males and 12 females per group at doses of 0.4 and 2 mg/kg. After the administration period, 5 males and 5 females at the 0, 10 and 50 mg/kg were placed on withdrawal for 2 weeks. Treatment-related clinical signs were observed at 10 and 50 mg/kg. Clinical signs observed immediately after the administration of DA-5018 were grooming, sedation or depression, lacrimation, atacia, reddening of extremities and ears, ventral or lateral recumvincy, respiratory distress, cyanosis and convulsion. Delayed-type clinical signs including focal scabbing and depilation around nose were also observed 1 or 2 weeks after the start of administration of DA-5018. Only at the 50 mg/kg group, corneal opacities, reduced body weight gain (male) and death (male 6/17, female 3/17) were noted. In blood biochemical analysis, serum levels of glucose and triglyceride decreased at 10 and 50 mg/kg. In hematological examination, there were increases in the number of red blood cell, hemoglobin content and percent of hematocrit at 10 and 50 mg/kg. Pulmonary enlargement and hemorrhagic spot, focal scabbing and depilation around nose and corneal opacities were seen at the necropsy of the animals died during the dosing of DA-5018 50 mg/kg. Focal scabbing and depilation around nose were observed in the animals terminally necropsied at doses of 10 and 50 mg/kg. Histopathological examination revealed pulmonary hemorrhage, focal necrosis in the scabbed area, corneal necrosis, fibrosis and neovasculization in the stroma. At 0.4 and 2 mg/kg, there were no significant toxic changes attributable to the administration of DA-5018. In conclusion, target organs following to 4-week repeated dose of DA-5018 in the rat were determined to be lung, skin and eyes. Definite toxic dose and no-observed-adverse-effect-level (NOAEL) were estimated to be 50 and 2 mg/kg/day, respectively.

• 한국임상수의학회지
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• 제31권2호
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• pp.159-162
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• 2014

4개월령 더러브렛 암컷 망아지가 하악 치은의 큰 종괴로 인해 내원하였다. 증식된 종괴는 단단하고 황색 기미의 분홍색을 띠고, 하악 잇몸 전체에 분포하고 있었다. 암망아지는 종괴로 인해 입술을 다물 수 없었고, 방사선상 아래턱의 종괴 음영이 확인되었다. 종괴는 전신마취상태에서 외과적으로 제거되었다. 병리조직학적으로 잇몸 종괴는 증식된 잇몸 상피, 혈관이 발달한 교원성 기질 및 많은 골조직으로 구성되어 있었다. 이와 같은 육안 및 병리조작학적 특징을 토대로 하악 잇몸에서 발생한 골화성 치은종으로 확진되었다. 본 증례는 한국에서 더러브렛 망아지에서 발생한 골화성 치은종의 첫 보고이다.

• 대한수의학회지
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• 제41권3호
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• pp.287-292
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• 2001

It has been known that the hemolymph node is one of the lymphoid organs found normally in ruminants and some rodents, and this organ shares morphological and functional characteristics of lymph node and spleen. To clanify the rigorous definition of morphological structures and functions of the hemolymph node in Wistar Kyoto(WKY)rats, we examined these organs of WKY rats gross anatomically and light microscopically. The hemolymph nodes were normally found in the abdominal cavity and in the neck of WKY rats. This organ was surrounded by a thin connective tissue capsule and there was a hilus. The parenchyma comprised a cortex of lymphatic nodules and diffuse lymphatic tissues, and a medulla of diffuse lymphatic tissues arranged in cords. Afferent and efferent lymph vessels were observed but there was no extensive subcapsular and medullary sinuses. These sinuses were filled with erythrocytes. The stroma of hemolymph nodes was composed of reticular cells and fibers, and many lymphocytes, granulocytes, erythrocytes, plasma cells, macrophages and megakaryocytes were supported by the reticular network. The above findings suggest that the hemolymph nodes of WKY rats may take part in blood formation, blood filtration and immune reaction.

• 대한수의학회지
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• 제52권4호
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• pp.269-273
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• 2012

In the present study, we investigated the expression patterns of ErbB family proteins in the pre-pubertal and pubertal mammary glands of dairy cows in association with gland development. For this study, we performed immunohistochemistry for ErbB-1-4 and Ki-67 cell proliferation marker. We found that the pre-pubertal and pubertal mammary glands had typical structures, including ducts and terminal end buds embedded in the stroma, and no development of lobuloalveolar structures. On immunohistochemistry, ErbB-2 and ErbB-3 were strongly expressed in the cytoplasm and nuclei in the epithelial cells of mammary ducts and terminal end buds, and stromal cells, whereas ErbB-1 and ErbB-4 were weakly expressed only in the cytoplasm of gland epithelium and stromal cells, irrespective of the developmental stage. Cell proliferation was inactive in the mammary gland cell compartments in both phases. Thus, expression of the ErbB family in the developing mammary glands was not associated with their functional effects, such as cell proliferation and lobuloalveolar development. In conclusion, ErbB receptors were differentially expressed in the epithelial and stromal cells of virgin mammary glands of dairy cows. Compared with rodent mammary glands, ErbB-3 and ErbB-4 were found to be highly expressed in bovine mammary glands.

• Tuberculosis and Respiratory Diseases
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• 제69권4호
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• pp.284-287
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• 2010

We report the case of a 68-year-old man with a stromal tumor of uncertain malignant potential (STUMP), which had metastasized to the lung. The patient complained of an enlarged mass in the anterior chest. Chest computed tomography (CT) showed a sternal abscess with multiple nodules in both lungs. A thoracoscopic lung biopsy of the nodules and incision/drainage of the sternal mass were performed simultaneously. CT of the pelvis revealed an enlarged prostate with irregular cystic lesions in the pelvis. Prostate biopsy was done and demonstrated hypercellular stroma with minimal cytological atypia, a distinct pattern of STUMP. The sternal abscess proved to be tuberculosis and the lung lesion was consistent with STUMP, which had spread from the prostate. However, to our knowledge, the tuberculous abscess might not be assoicated with STUMP in the lung. The patient refused surgical prostatectomy and was discharged with anti-tuberculosis medication. On one-year follow up, the patient had no evidence of disease progression.

• 대한두개안면성형외과학회지
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• 제11권1호
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• pp.62-64
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• 2010

Purpose: Lobular capillary hemangioma is a vascular tumor that commonly occurs as a cutaneous lesion. Intravenous pyogenic granuloma is a rare form of lobular capillary hemangioma that usually occurs in the veins of the neck and the upper extremities. Methods: A 41-year-old man presented with 3 months history of nodules on the left temporal area and these lesions were clinically mistaken for a typical lipoma or epidermal cyst, but the nodules appeared unusually vascular with sentinel veins on excision. Results: On the histopathologic examination, the excised tissue was observed as an intraluminal polypoid mass, which was attached to the vein via a fibrovascular stalk and capillaries in a loose edematous fibromyxoid stroma, and so the lesions were confirmed to be intravenous pyogenic granuloma. At 6 months after excision, there has been no recurrence and the patient is asymptomatic. Conclusion: Intravenous pyogenic granuloma is a rare variant of lobular capillary hemangioma. This article describes the surgical and histopathological findings of treating pyrogenic granuloma.

• Journal of Chest Surgery
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• 제40권11호
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• pp.789-792
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• 2007

양성 전이성 평활근종은 조직학적으로는 양성이나 임상적으로 폐나 다른 장기로 원격전이를 하는 매우 드문 질환이다. 저자들은 10년 전 자궁평활근종 절제술을 시행한 환자의 폐에서 발견된 다발성 결절 일부를 흉강경 수술하에 절제하여 조직학적 및 면역염색검사로 양성 전이성 평활근종임을 진단할 수 있었다. 환자는 수술 후 종양의 크기 변화 및 특이 증상이 없으며 특별한 치료 없이 외래 추적관찰 중이다.

• 대한세포병리학회지
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• 제7권1호
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• pp.97-102
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• 1996

Fine needle aspiration cytology of a pulmonary mass was performed on a 51-year-old man who had a left testicular mass. Cytologic features were composed of a homogeneous population of malignant cells associated with a background of foamy and lacelike material. The cellular features were characterized by monomorphous cell proliferation of relatively regular large cells, generally isolated or grouped. Occasionally, fine blanching stroma with large tumor cells and scanty lymphocytes were noted. The tumor cells had a round, regular nucleus, prominent round nucleoli, and a thin rim of cytoplasm containing large vacuoles or lacunae filled with glycogen. The fine needle aspiration cytologic diagnosis was highly consistent with metastatic seminoma from testis and less likely primary or other metastatic carcinoma. The diagnosis of resected testicular mass was classic seminoma. Despite the fact that cytopathologists were not familial with diagnosis of seminoma due to clinician's lack of interest in fine needle aspiration cytology of germ cell tumors including seminoma, it appears that a diagnosis of this tumor should not be problematic in cytologic material if specific histologic criteria are applied.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.19-19
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• 2003

The Xylariaceae Tul. & C. Tul is a family of sphaeriaceous genera with obscure but apparently common ancestry (Rogers, 1979), A central core of genera - Xylaria, Hypoxylon, Rosellinia, Poronia, Podosordaria, Hypocopra, Daldinia, Biscogniauxia, Kretzschmaria, Camillea, Penzigia - are obviously related, regardless of the fact that the generic limits are arguable (Rogers, 1979; Barr, 1990; Eriksson & Hawksworth, 1991; Laessoe, 1994). In general, the family Xylariaceae is primarily defined as a group of stromatic pyrenomycetes with unitunicate asci characterized by a typical apical apparatus and pigmented ascospores having a germ slit (Rogers, 1979). Since Winter (1887) circumscribed the family to include Pyrenomycetes with predominately a dark stroma, dark unveiled spores containing 5 genera: Nummularia Tul. & C. Tul., Hypoxylon Bull., Ustulina Tul. & C.Tul., Poronia Willd. and XylariaHill ex Schrank, many more genera has been added to the family (Dennis, 1961; Eriksson & Hawksworth, 1993; Whalley, 1996). In the absence of a clear circumscription of the family (Rogers, 1994) the agreed number of accepted genera is governed by individual views and there are therefore difference between the proposals for the ascomycetes recognized 35 genera and indicated a further 3 which might belong there. Laessoe reviewed the family and included 37 genera but a few of these were listed as uncertain (Laessoe, 1994). In the most recent accounts Whalley (1996) listed 41 genera but again a number of these were considered uncertain and Ju and Rogers (1996) accepted 39 genera in their interpretation of the family. Since Laessoe (1994) merged Daldinia and Versiomyces with Hypoxylonand Ju and Rogers (1996) combined Astrocystis with Rosellinia and Helicogermslita, which can be argued strongly against, it is likely that a realistic number of genera will be close to 40 (Whalley, 1996; Table 1).