• Molecules and Cells
• /
• 제19권1호
• /
• pp.74-80
• /
• 2005

Stress is known to inhibit granule cell proliferation in the hippocampus. However, recent studies suggest that the commonly used dose of bromodeoxyuridine (BrdU) is insufficient to label all fractions of granule cells. Furthermore, stress-induced changes in BrdU availability may influence the labeling of newly born cells. To investigate whether changes in BrdU availability affect measurements of stress-induced granule cell proliferation, granule cell proliferation was assessed using injection of high doses of BrdU before and after restraint stress lasting 1 h. In addition, to determine whether stress-induced changes in plasma corticosterone levels were influenced by the BrdU, time-dependent changes in plasma corticosterone levels over 2 h after BrdU injection were compared with total accumulated plasma corticosterone levels [as determined by areas under the curve (AUC)]. Restraint stress significantly reduced the numbers of BrdU-labeled cells and clusters in the granule cell layer (GCL) of rats that received BrdU after stress, and decreases of similar magnitude were observed when the rats were given BrdU before stress. BrdU injection enhanced the stress-induced plasma corticosterone response, but there was no difference between the mean AUCs of plasma corticosterone levels of animals injected with BrdU before or after stress. These observations suggest that restraint stress decreases granule cell proliferation, and that this may be influenced by the extent and duration of plasma corticosterone increases rather than by changes in the availability of BrdU.

• Water Engineering Research
• /
• 제3권3호
• /
• pp.155-162
• /
• 2002

This study presents the theoretical approach for volume concentration, velocity profile, and granular discharge on the fluid-granule mixed flow downstream of the scour hole at the outlet of the hydraulic structure. Concept of dilatant model was applied for the stress-strain relationships of fluid-granule mixed flow since the flow downstream of the scour hole corresponds to debris flow, where momentum transfers through particle collisions. Mathematical formulations were derived using momentum equation and stress-strain relation of the fluid-granule mixture. Velocity profile under the assumption of uniform concentration over flowing layer showed the downward convex type. Deposition angle of downstream hump was found to be a function of an upstream slope angle, a dynamic friction angle and a volume concentration irrespective of flow itself, Granular discharge and the overflow depth were obtained with given values of inflow rates. Experimental results showed relatively good agreements with theoretical ones.

• Asian-Australasian Journal of Animal Sciences
• /
• 제22권2호
• /
• pp.246-253
• /
• 2009

This study was conducted to investigate the effects of Chinese medicine granule (CMG, including Cortex Phellodendron, Atractylodes Rhizome, Agastache Rugosa and Gypsum Fibrosum) on absorption and transport of glucose in porcine small intestinal brush border membrane vesicles (BBMVs) under heat stress. Forty-eight 2-month-old Chinese experimental barrows were screened according to weight and litter origin, and then allotted to three groups and treated as follows: Normal temperature control group (NTCG; $23^{\circ}C$), high temperature control group (HTCG; $26^{\circ}C$ for 19 h, $40^{\circ}C$ for 5 h); Chinese medicine granule anti-stress group (CMGG; $26^{\circ}C$ for 19 h, $40^{\circ}C$ for 5 h) (n = 16 per group). The results showed that high temperature treatment decreased (p<0.05) the growth performance and intestinal glucose absorption but there was no change (p>0.05) in the expression of SGLT1 and GLUT2 genes in the small intestine of pigs compared with the NTCG. Dietary supplementation with CMG improved the growth performance, and increased the activity of disaccharidases in duodenum and jejunum of heat stressed pigs (p<0.05). CMG treatment increased (p<0.05) the protein levels of SGLT1 and GLUT2 in the small intestine, and up-regulated (p<0.05) the expression of SGLT1 and GLUT2 genes in the duodenum and jejunum but without changing (p>0.05) them in the ileum compared with the HTCG. These results indicated that CMG treatment significantly improved porcine growth performance, and increased intestinal glucose absorption and transport by BBMVs under heat stress, in addition to up-regulating the expression of SGLT1 and GLUT2 genes in porcine duodenum and jejunum.

• Molecules and Cells
• /
• 제46권8호
• /
• pp.486-495
• /
• 2023

Lipofuscins are oxidized lipid and protein complexes that accumulate during cellular senescence and tissue aging, regarded as markers for cellular oxidative damage, tissue aging, and certain aging-associated diseases. Therefore, understanding their cellular biological properties is crucial for effective treatment development. Through traditional microscopy, lipofuscins are readily observed as fluorescent granules thought to accumulate in lysosomes. However, lipofuscin granule formation and accumulation in senescent cells are poorly understood. Thus, this study examined lipofuscin accumulation in human fibroblasts exposed to various stressors. Our results substantiate that in glucose-starved or replicative senescence cells, where elevated oxidative stress levels activate autophagy, lipofuscins predominately appear as granules that co-localize with autolysosomes due to lysosomal acidity or impairment. Meanwhile, autophagosome formation is attenuated in cells experiencing oxidative stress induced by a doxorubicin pulse and chase, and lipofuscin fluorescence granules seldom manifest in the cytoplasm. As Torin-1 treatment activates autophagy, granular lipofuscins intensify and dominate, indicating that autophagy activation triggers their accumulation. Our results suggest that high oxidative stress activates autophagy but fails in lipofuscin removal, leaving an abundance of lipofuscin-filled impaired autolysosomes, referred to as residual bodies. Therefore, future endeavors in treating lipofuscin pathology-associated diseases and dysfunctions through autophagy activation demand meticulous consideration.

• 한국식품과학회지
• /
• 제21권6호
• /
• pp.755-759
• /
• 1989

2종의 감자전분(세풍과 대지)로부터 각각 큰 입자 $(41{\mu}m$ 이상)과 작은 입자$(30{\mu}m$ 이하)를 분획하고 각 입자 크기별 가열 호화액에 대한 리올로지 특성을 분획전의 전분과 비교하면서 검토하였다. 시료 호화액은 모두 빙햄의가소성의 거동을 보였고 이 거동은 품종 또는 입자크기간의 차이를 보이지 않았으며 온도의 영향도 없었으나 농도의 증가로 의가소성의 성질은 다소 강해졌다. 점조도지수 및 항복응력값은 품종간의 차이는 없었으나 입자크기간에는 현저한 차이를 보여서 각 대응하는 농도에서 작은 입자의 경우가 큰 입자보다 훨씬 큰 점조도지수 및 항복응력값을 보였고 농도증가로 지수함수적으로 증가하였다. 또, 이들 값은 측정온도에 대하여 품종 및 입자크기에 따라 차이를 보이면서 온도증가로 감소하였고, 작은 입자>분획전의 전분입자>큰 입자의 순으로 온도의존성을 되었다.

• 스트레스연구
• /
• 제26권4호
• /
• pp.305-311
• /
• 2018

화병은 뚜렷한 스트레스 사건과 관련되어 가슴의 답답함, 열감, 치밀어 오름, 억울하고 분한 감정 등이 나타나는 증후군이다. 본 논문에서는 화병에 대한 황련해독탕의 임상시험 결과를 교감신경의 활성도를 반영한다고 알려져 있는 심박변이도의 저주파(0.04~0.15 Hz) 영역 값을 중심으로 분석하여 다음과 같은 결과를 얻었다. 첫째, 황련해독탕 시험군에서 초기 심박변이도 저주파 값이 클수록 불면 증상의 개선 정도가 작게 나타났다. 둘째, 위약과 비교했을 때, 황련해독탕은 복용 7일 후 심박변이도 저주파 값을 유의하게 감소시켰다. 셋째, 화병 증상 중 가슴이 답답함, 치밀어 오름, 분노가 심박변이도 저주파 값과 유의미한 상관성이 있었다. 이를 바탕으로 심박변이도는 화병 환자를 평가할 때 유용할 것이라고 기대된다.

• 통합자연과학논문집
• /
• 제12권4호
• /
• pp.127-132
• /
• 2019

Programmed cell death 4 (PDCD4) is a novel tumor suppressor that function in the nucleus and the cytoplasm and appears to be involved in the regulation of transcription and translation. Stress granules (SGs) are cytoplasmic foci at which untranslated mRNAs accumulate when cells exposed to environmental stresses. Since PDCD4 has implicated in translation repression through direct interaction with eukaryotic translation initiation factor 4A (eIF4A), we here investigated if PDCD4 has a functional role in the process of SG assembly under oxidative stresses. Using immunofluorescence microscopy, we found that PDCD4 is localized to SGs under oxidative stresses. Next, we tested if knockdown of PDCD4 has an effect on the assembly of SG using PDCD4-specific siRNA. Interestingly, SG assembly was accelerated and this effect was caused by sensitization of phosphorylation of eIF2α and dephosphorylation of eIF4E binding protein (4E-BP). These results suggest that PDCD4 has an effect on SG dynamics and possibly involved in cap-dependent translation repression under stress conditions.

• BMB Reports
• /
• 제52권3호
• /
• pp.196-201
• /
• 2019

The Alu element, the most abundant transposable element, is transcribed to Alu RNA. We hypothesized that the PIWI protein regulates the expression of Alu RNA in retinal pigment epithelial (RPE) cells, where accumulated Alu RNA leads to macular degeneration. Alu transcription was induced in RPE cells treated with $H_2O_2$. At an early stage of oxidative stress, PIWIL4 was translocated into the nucleus; however, subsequently it was sequestered into cytoplasmic stress granules, resulting in the accumulation of Alu RNA. An elevated amount of Alu RNA was positively correlated with the disruption of the epithelial features of RPE via induction of mesenchymal transition. Therefore, we suggest that oxidative stress causes Alu RNA accumulation via PIWIL4 sequestration into the cytoplasmic stress granules.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 2004년도 Annual Meeting of the Korean Society ofApplied Pharmacology
• /
• pp.124-128
• /
• 2004

We have assessed amyloid ${\beta}-peptide$ $(A{\beta})-induced$ neurotoxicity in primary neurons and organotypic hippocampal slice cultures (OHC) in rat. Exposing cultured hippocampal and cerebellar granule neurons to $A{\beta}$ resulted in a decrease of MTT reduction, and in destruction of neuronal integrity. Treatment of these neurons with tunicamycin, an inhibitor of N-glycosylation in the endoplasmic reticulum (ER), also decreased MTT reduction in these neurons. S-allyl-L-cysteine (SAC), an active organosulfur compound in aged garlic extract, protected hippocampal but not cerebellar granule neurons against $A{\beta}$- or tunicamycin-induced toxicity. In the hippocampal neurons, protein expressions of casapse-12 and GRP 78 were significantly increased after $A{\beta}_{25-35}$ or tunicamycin treatment. The increase in the expression of caspase-12 was suppressed by simultaneously adding $1{\mu}M$ SAC in these neurons. In contrast, in the cerebellar granule neurons, the expression of caspase-12 was extremely lower than that in the hippocampal neurons, and an increase in the expression by $A{\beta}_{25-35}$ or tunicamycin was not detected. In OHC, ibotenic acid (IBO), a NMDA receptor agonist, induced concentration-dependent neuronal death. When $A{\beta}$ was combined with IBO, there was more intense cell death than with IBO alone. SAC protected neurons in the CA3 area and the dentate gyrus (DG) from the cell death induced by IBO in combination with $A{\beta}$, although there was no change in the CA1 area. Although protein expression of casapse-12 in the CA3 area and the DG was significantly increased after the simultaneous treatment of AI3 and IBO, no increase in the expression was observed in the CA1 area. These results suggest that SAC could protect against the neuronal cell death induced by the activation of caspase-12 in primary cultures and OHC. It is also suggested that multiple mechanisms may be involved in neuronal death induced by AI3 and AI3 in combination with IBO.

• 한국수자원학회논문집
• /
• 제30권6호
• /
• pp.661-669
• /
• 1997

본 연구에서는 홍수시 비점착성 제체 위를 월류하는 흐름에 의한 유체-고체 혼합류의 속도 분포와 판박형 세굴을 다루고 있다. 속도 분포는 입지-관성 법칙을 기초로 한 응력-변형률 관계식으로부터 구할 수 있었으며, 세굴 깊이는 Coulomb의 동역학적 법칙을 이용하여 구할 수 있었다. 상기 이론으로부터 구한 이론치를 검토하기 위해 실험을 실시하였으며, 실험치와 비교적 잘 일치함을 알 수 있었다. 본 연구에서 얻어진 속도 분포의 이론식은 유체-고체 혼합류의 여러 유속 분포에 모두 적용 가능한 것으로서, 토석류에 관한 기존 이론을 상당히 개선시킬 수 있는 식으로 평가되었다. 설계 목적을 위해서, 만약 홍수량과 입자의 성질 및 제체의 규격이 주어진다면, 속도 분포와 세굴 깊이, 월류 수심 및 토사 유출량을 구할 수 있는 식 및 도표를 제시하였다.