• Molecules and Cells
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• 제37권11호
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• pp.833-840
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• 2014

Cullin4-RING ubiquitin ligase (CRL4) is a family of multi-subunit E3 ligases. To investigate the possible involvement of CRL4 in heat stress response, we screened T-DNA insertion mutants of putative CRL4 substrate receptors that exhibited altered patterns in response to heat stress. One of the mutants exhibited heat stress tolerance and was named heat stress tolerant DWD1 (htd1). Introduction of HTD1 gene into htd1-1 led to recovery of heat sensitivity to the wild type level, confirming that the decrease of HTD1 transcripts resulted in heat tolerance. Therefore, HTD1 plays a negative role in thermotolerance in Arabidopsis. Additionally, HTD1 directly interacted with DDB1a in yeast two-hybrid assays and associated with DDB1b in vivo, supporting that it could be a part of a CRL4 complex. Various heat-inducible genes such as HSP14.7, HSP21, At2g03020 and WRKY28 were hyper-induced in htd1-1, indicating that HTD1 could function as a negative regulator for the expression of such genes and that these genes might contribute to thermotolerance of htd1-1, at least in part. HTD1 was associated with HSP90-1, a crucial regulator of thermotolerance, in vivo, even though the decrease of HTD1 did not affect the accumulation pattern of HSP90-1 in Arabidopsis. These findings indicate that a negative role of HTD1 in thermotolerance might be achieved through its association with HSP90-1, possibly by disturbing the action of HSP90-1, not by the degradation of HSP90-1. This study will serve as an important step toward understanding of the functional connection between CRL4-mediated processes and plant heat stress signaling.

• 한국산학기술학회논문지
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• 제15권7호
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• pp.4093-4099
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• 2014

본 논문에서는 200 kW급 마이크로 가스 터빈 시스템의 연소기집합체의 열-구조 연성 해석을 수행하였다. 일반적인 연소기집합체는 라이너, 내외 케이스, 버너와 노즐링 등으로 구성되어 있으며, 본 연구에서 개발된 유한요소모델은 연소기집합체 내 다양한 부품들의 다른 열팽창을 보상하기 위해 부품간의 이격과 마찰 요소들이 존재하므로 비선형 간극 및 마찰 요소 등을 포함하고 있다. 본 연구를 통해 연소기집합체의 외부 경계지지조건이 높은 온도구배로 인한 응력 에 가장 큰 영향을 주는 인자라는 것을 밝혀냈으며, 과도한 응력이나 변형을 방지하기 위한 적절한 외부 경계지지조건을 찾기 위하여 외부 경계지역에 탄성 지지조건을 가상하여 적절한 경계조건을 도출하여 설계에 사용될 수 있도록 하였다.

• 한국산학기술학회논문지
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• 제21권2호
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• pp.373-380
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• 2020

고압을 받는 플랜트용 제품 설계 시에 대부분 강건 구조를 지양하다 보면 안전율이 높아지고 중량이 과다 해지는 문제가 있다. 본 논문은 플랜트용 이축 볼밸브 볼의 경량화를 위하여 밸브의 3차원 모델링과 ANSYS를 적용한 유한요소해석으로 응력해석을 수행한 후에, 허용 응력 범위와 설계 변경 제한 조건 내에서 최적화 과정으로 볼의 중량 감소를 달성하였다. 약 22%의 볼 중량이 감소되었고 응력은 증가하였으나 안전계수는 1.25가 되었다. 볼의 일부분에 과도한 응력이 있으나 대부분 재료의 항복응력 허용치 내에 있어 안전한 상태이다. 유동의 흐름을 제어하는 실링 (seal) 역할을 하는 시트 인서트와 시트 링 등 밸브의 3차원 모델에 대한 정적 구조해석으로 응력 값을 확인하여 안전함을 확인하였고, 또한 대칭 구조인 부분에 대해 2차원 단순 모델의 구조해석으로 같은 결과가 도출됨을 확인하였다. 본 연구의 범위는 볼의 중량 감소라 볼의 감소된 치수가 도출되었으며, 주변 부재의 재설계는 (신제품 개발에 해당되어) 본 연구에 고려되지 않는다.

• 대한조선학회논문집
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• 제47권1호
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• pp.67-75
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• 2010

Submarine and deep sea diving structures are generally designed based on their ultimate strength. Fatigue strength at welded joint must be also taken into account because working stress is increased due to the increasing of diving depth and using high yield steel. The pressure hulls of submarine are subjected to fluctuating compressive loading. But in addition to the calculated stresses, high residual tensile stresses at welded part have to be considered. The state of stress level of pressure hull is tensile at surface and compressive at deep diving depth. This paper presents the results of an experimental investigation on the crack initiation and growth at the weld toe of T welded joints of HY-100 steel plate under constant amplitude loading. It is also investigated the phenomenon of the fatigue failure and test methods. Fatigue tests have been using real scaled local structural models of full penetration T-welded joint, which is a part of the cylindrical shell structures reinforced by ring stiffeners. Several load ratios under constant amplitude loading are considered in the tests. Crack initiation and growth characteristics are examined based on the beach marks of the cracked section of the test specimens. A design stress-life curve including the design formula is suggested according to tested data.

• 한국재료학회지
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• 제7권1호
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• pp.62-68
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• 1997

본 연구에서는 접초피로에 있어서 균열의 발생, 진전 등의 관찰을 위해, 균열의 발생, 진전 등이 2차원적으로 되어 시험편측면에서 관찰이 가능한 평판 ring형 시험편을 이용하여 반복수 증대에 따른 균열의 발생, 진전과정을 조사하였다. 그 결과 pitting, flaking형 파손의 초기손상은 접촉면하의 내부에 생기는 접촉면에 평행방향의 균열에 의해 일어나며, 이 균열은 그 방향 밀 파면형태에 의해 접촉응력이 접촉면에 평행방향의 전단응력성분에 의한 모드 ll 피로진전과의 차는 중첩부하된 압축응력의 유무라고 생각되며, 이 가저에 근거로 하여 재료고유의 모드 ll 피로균열진전특성을 구할 수 있는 장치를 개발하였다. 이 장치를 이용하여 알루미륨합금 및 공구강에 대한 da/dN-${\Delta}k$ ll 관계의 시험결과를 얻었다.

• 한국축산식품학회지
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• 제44권1호
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• pp.132-145
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• 2024

Sarcopenia, the age-related muscle atrophy, is a serious concern as it is associated with frailty, reduced physical functions, and increased mortality risk. Protein supplementation is essential for preserving muscle mass, and horse meat can be an excellent source of proteins. Since sarcopenia occurs under conditions of oxidative stress, this study aimed to investigate the potential anti-muscle atrophy effect of horse meat hydrolysate using C2C12 cells. A horse meat hydrolysate less than 3 kDa (A4<3kDa) significantly increased the viability of C2C12 myoblasts against H₂O₂-induced cytotoxicity. Exposure of C2C12 myoblasts to lipopolysaccharide led to an elevation of cellular reactive oxygen species levels and mRNA expression of proinflammatory cytokines, including tumor necrosis factor-α and interleukin 6, and these effects were attenuated by A4<3kDa treatment. Additionally, A4<3kDa activated protein synthesis-related proteins through the protein kinase B/mechanistic target of rapamycin pathway, while decreasing the expression of activity and degradation-related proteins, such as Forkhead box O3, muscle RING finger protein-1, and Atrogin-1 in dexamethasone-treated C2C12 myotubes. Therefore, the natural material A4<3kDa has the potential of protecting against muscle atrophy, while further in vivo study is needed.

• Steel and Composite Structures
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• 제19권1호
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• pp.75-92
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• 2015

In this paper, optimization of cylindrical shells under external pressure to minimize its weight has been studied. Buckling equations are based on standard of ABS underwater vehicles. Dimension and type of circumferential stiffeners, and its distance from each other are assumed as variables of optimization problem. Considering the extent of these variables, genetic algorithms have been used for optimization. To study the effect of hydrostatic pressure on the shell and its fabrication according to the existing standards, geometrical and construction as well as stress and buckling constraints have been used in optimization algorithm and also penalty functions are applied to eliminate weak model. Finally, the best model which has the minimum weight considering the applied pressure has been presented.

• 한국자동차공학회논문집
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• 제6권3호
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• pp.106-114
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• 1998

Cogging torque is often a principal source of vibration and control difficulty in permanent magnet motors, especially at low speeds and loads. For example, reduction of cogging torque is an important specification for DC motors used for electric power stee- ring. This paper examines two motor design techniques, stator tooth notching and rotor pole skewing with magnet pole shaping, for reduction of cogging torque, and the effect of each method on the airgap flux, and the use of the Maxwell stress method and Fourier decomposition to calculate the periodic cogging torque. The analyses show that the cogging torque can be nearly eliminated by the suggested designs, with minimal scacrifice of output torque.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1999년도 가을 학술발표회 논문집
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• pp.59-66
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• 1999

ILT proposed by Tezaghi was frequently used which is based on one dimensional consolidation theory. But this test require time longer than a week and has problems for extra soft clay such as the squeezing around the consolidation ring. Also consolidation curve is not clearly defined since only a few data is obtained in a test. Therefore it is difficult to determine Pc and the interpretation to determine the consolidation constants are rather complicated. In this paper, the stress-strain relationship and consolidation constant obtained by CRS and CG-test were analyzed and compared with the results by ILT.

• 한국생산제조학회지
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• 제18권3호
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• pp.321-327
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• 2009

This paper studied the influences of the main design parameter-the expansion angle and the material properties of the self-expansion anterior cruciate ligament fixation device on the contact condition with the bone and the initial stability of the device. Using finite element analysis, the stress distributions of the ring part of the device and the wall of the bone tunnel were calculated. And the micro-migration of the device by the pull-out force was calculated. From the analysis results, it was found that when designing the self-expansion type anterior cruciate ligament fixation device, it is desirable to use the material having higher Young's modulus and to design the fixation device that all wedges uniformly maintain contact with bone to obtain initial stability after operation.