• Title/Summary/Keyword: Stress, Physiological

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Environmental stress-related gene expression and blood physiological responses in olive flounder (Paralichthys olivaceus) exposed to osmotic and thermal stress

  • Choi, Cheol-Young
    • Animal cells and systems
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    • v.14 no.1
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    • pp.17-23
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    • 2010
  • We isolated warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of olive flounder and investigated the mRNA expression of Wap65 and HSP70 in olive flounder exposed to osmotic (17.5, 8.75, and 4 psu) and thermal stress (25 and $30^{\circ}C$). The mRNA expression of Wap65 and HSP70 was increased by thermal stress. The mRNA expression of HSP70 was also increased by osmotic stress, whereas no significant change in Wap65 expression was detected. These results indicate that Wap65 mRNA expression occurs specifically in response to increases in water temperature, but not in response to osmotic stress. Plasma cortisol levels were also increased by osmotic and thermal stress. We also utilized the stress hormone cortisol to examine whether Wap65 expression is thermal-stress-specific. Cortisol treatment increased HSP70 mRNA expression in vitro, but had no significant effect on Wap65 mRNA expression. Thus, thermal stress, but not osmotic stress, induces Wap65 expression.

Physiological responses of selected Philippine upland rice genotypes evaluated using drought and salinity stress

  • Zapico, Florence;Aguilar, Catherine Hazel;Laniton, Lyn Jean;Lincay, Reygiene;Duldoco, Roman Abdul Kadir;Leandres, Jacy Deneb
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.306-306
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    • 2017
  • Screening for drought and salinity tolerance was undertaken for selected Philippine upland rice landraces during germinative and seedling stages to identify varieties which can potentially be grown in marginally dry and saline soils. While increasing PEG and NaCl concentrations caused obvious signs of injury to all rice genotypes, considerable varietal differences were noted in the nature of responses providing evidence that these genotypes possess broad intraspecific genetic variations for drought and salt tolerance. Inconsistent responses of these varieties during both growth stages highlight complexities involved in stress responses and underscore the futility of utilizing a single stage in the rice plant's life cycle for physiological screening. Notwithstanding these perplexing responses, G_Katiil and Ml-Pilit Tapul were observed to thrive relatively well despite increased salt and drought stress during early growth stages and may therefore possess genes needed in crop improvement efforts for drought and salinity tolerance. While these results do not reflect the entire spectrum of adaptive expression to drought and salinity stress during the life cycle of the upland rice plant, they nonetheless provide an easy, reliable and reproducible method for preliminary identification of drought and salt tolerant rice varieties.

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A Suggestion on Evaluating Personal Health State: Health Index (건강상태 평가를 위한 지수 개발: 헬스인덱스)

  • Moon, Dong-Ju;Lee, Sung-Il;Lee, Chong-Sun;Kim, Gyeong-Cheol;Kang, Hee-Jung;Yang, Yong-Ju
    • Journal of Biomedical Engineering Research
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    • v.29 no.5
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    • pp.400-407
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    • 2008
  • A health index was proposed that evaluates personal health state from both measured physiological variables and survey questions. Four health indices were defined such as cardiovascular index, stress index, obesity index, and management index. The total health index was calculated by summing these four health indices. Physiological variables such as blood pressure, heart rate variability(HRV), accelerated photoplethysmograph(APG), and body fat percentage were non-invasively measured and a survey questionnaire that asks personal health state, exercise intensity, and food preference was developed. The suggested health index was applied to thirty eight persons including 30 patients and 8 normal persons with an average age of 51.8. The average health index was estimated to be 75.1 out of 100 points. Young age group(below 50) and men group showed higher health indices than the aged(over 50) and women groups. The correlation coefficient between the cardiovascular index and stress index was found to be 0.513, which means stress is related to cardiovascular health state. The correlation coefficient between the measurements and survey questions was 0.385 for the cardiovascular index. It was as low as 0.182 for the stress index. More case studies may improve correlations between measurements and survey questions, and then, the current health index system may develop as an effective tool to evaluate personal health state.

Effects of transport stress on physiological responses and milk production in lactating dairy cows

  • Hong, Heeok;Lee, Eunchae;Lee, In Hyung;Lee, Sang-Rak
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.3
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    • pp.442-451
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    • 2019
  • Objective: This study was conducted to investigate the effect of transport stress on physiological and hematological responses and milk performance in lactating dairy cows. Methods: Ten lactating dairy cows were randomly divided into 2 groups. The treatment group (TG) was transported 200 km for 4 h by truck, and the control group (NTG) was restrained by stanchion for 4 h in Konkuk University farm. Blood and milk samples were collected at 24 h pre-transport; 1, 2, and 4 h during transport; and 2, 24, and 48 h post-transport. Milk yields were measured at 24 h pre-transport, 0 h during transport, and 24, 48, and 72 h post-transport. Results: Leukocyte, neutrophil, and monocyte numbers in the TG were significantly higher than those of the NTG at each experimental time point. Lymphocyte numbers in the TG were significantly (p<0.05) higher than those of the NTG at 48 h post-transport. Additionally, the neutrophil:lymphocyte ratio of the TG was 45% and 46% higher than that of the NTG at 4 h during transport and 2 h post-transport, respectively. There were no significant differences in erythrocyte numbers, hemoglobin concentrations, platelet numbers, and hematocrit percentages between two groups. Cortisol levels in the TG were significantly (p<0.05) higher than those in the NTG. Milk yields in the TG were lower than those in the NTG. The somatic cell count (SCC) of the TG was significantly (p<0.05) higher than that of the NTG at 1 and 2 h during transport; that of the TG increased dramatically at 1 h during transport and gradually decreased subsequently. Conclusion: Transport stress increased blood parameters including leucocyte, neutrophil, and monocyte numbers by increased cortisol levels, but did not affect erythrocytes, hemoglobin and hematocrit levels. Additionally, transport resulted in a decrease in milk yield and reduced milk quality owing to an increase in milk SCC.

Reduced Physio-psychological Stress Responses to the Green Wall in Subway Station (지하철 역사 내 벽면녹화가 생리심리학적 스트레스 완화에 미치는 영향)

  • Kim, YongJin;Kang, Minji;Chung, LeeBom;Youn, ChoHye;Jeon, SeongMin;Lee, Juyoung
    • Journal of Environmental Science International
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    • v.31 no.3
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    • pp.219-226
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    • 2022
  • This study was conducted to investigate the response of the human body to stress induced by wall recording of subway stations in the city center. The experiment was conducted as a simulation exercise, and six images were selected and produced based on Subway Line 2, a representative underground space in Seoul. The study participants included 24 male and female college students. A three-minute experiment was conducted, during which the participants were shown the control image and green wall image once each. To measure psychological status, the following measurement indicators were used: Semantic Differential, Positive Affect and Negative Affect Schedule and State-Trait Anxiety Inventory. Physiological changes were investigated by tracking participants' heart rate and blood pressure. Results showed that parasympathetic and sympathetic nerves were activated in the presence of the green wall in the subway station. The psychological evaluation analysis revealed that negative affect toward underground space decreased, while positive affect increased. This study found that the green wall in subway stations has a stable effect on the human body, both psychologically and physiologically. In the future, green walls in underground spaces can be used to reduce psychological stress and increase physiological relaxation.

A Study of The Related Factors to Successful Weaning from Mechanical Ventilation in leu Patients (인공호흡기 치료환자의 이탈(weaning) 성공 관련 요인에 대한 연구)

  • Kim, Eun-Sung;Choi, S-Mi
    • Journal of Korean Biological Nursing Science
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    • v.9 no.1
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    • pp.71-84
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    • 2007
  • Purpose : The Purpose of this study was to identify the physiological, psychological factors to successful weaning of mechanical ventilation in ICU patients. Method: Data was collected by interview with the use of questionnaires from 70 ICU patients, who were mechanically ventilated more than 3days at Y university medical center. Data was analyzed with descriptive statics, t-test, and $x^2$ test, and Pearson Coefficient Correlation using SPSS WIN 12.0 program. Result: ICU patients with mechanical ventilator during weaning period reported high levels of anxiety, moderate levels of stress, and fatigue. The physiological, psychological factors influencing ventilator weaning success were RSBI(p=0.007), stress(p=0.009), anxiety(p=0.020), depression(p=0.040), fatigue(p=0.001), and dyspnea(p=0.010). The RSBI factor was shown to have positive correlations with ventilator care periods, $PaCO_2$, and fatigue. Dyspnea was also positively correlated with stress, anxiety, depression, and fatigue. Conclusion: This study suggests that RSBI, stress, anxiety, depression, fatigue, and dyspnea are significant factors to successful weaning from mechanical ventilation.

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Analysis of O-J-I-P Transients from Four Subtropical Plant Species for Screening of Stress Indicators under Low Temperature (저온스트레스 지표 선발을 위한 아열대성 식물 4종의 O-J-I-P곡선 분석)

  • Oh Soonja;Koh Seok Chan
    • Journal of Environmental Science International
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    • v.14 no.4
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    • pp.389-395
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    • 2005
  • The changes of O-J-I-P transients were investigated using leaves of four subtropical plant species (Crinum asiaticum var. japonicum, Osmanthus insularis, Chloranthus glaber and Asplenium antiquum) under the natural conditions in winter, in order to select the stress indicators for diagnosing physiological states of plants under low temperature. In the O-J-I-P transients of these species, the fluorescence intensity was found to be higher in O-step and lower in P-step in winter than in summer. Particularly, the fluorescence intensity of the P-step in Crinum asiaticum var. japonicum was lower than those of other three plant species, indicating that Crinum asiaticum var. japonicum is the most sensitive to low temperature. Of the chlorophyll fluorescence parameters derived from O-J-I-P transients of four subtropical plants, Fm, Fv/Fo, ABS/CS, TRo/CS, $\phi_{po}$ and $\phi_{po}/(1-\phi_{po})$ decreased significantly with the increase of Fo, Sm, N, EToICS, ETo/RC and $\psi_o/(1-\psi_o)$ depending on temperature drop in winter. Therefore, these parameters could be used as indicators for estimating low temperature stress and diagnosing physiological states of plants under the natural conditions in winter.

Effects of Bambusae Caulis in Liquamen on the Stress Proteins Induced by Heating in Endothelial Cells (혈관내피세포에 열 충격 부과시 죽력이 stress proteins의 발현에 미치는 영향)

  • Jeon Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.2
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    • pp.496-499
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    • 2004
  • We have previously observed that Bambusae Caul is in Liquamen (BCL) stimulates the adipose conversion of 3T3-L1 cells and molecular chaperones were involved in the process of the assembly and replacement of laminin subunits in Bovine aortic endothelial cells(BAEC). Endothelial cells are exposed to continuous shear stress due to the blood flow. Heat shock protens(hsp) are a well-known stress response protein, namely, stress proteins. To investigate effects of BCL on the stress proteins induced by heating in endothelial cells, we have analyzed synthetic amounts of stress proteins in sodium dodecyl sulfate gel electrophoresis under reducing conditions. Under the condition of heating stress, BCL inhibited the synthesis of stress proteins in endothelial cells. These results suggest that BCL may have an important role for expression of stress proteins induced by heating in endothelial cells.

A Literature Reviewed of Job Stress (직무스트레스에 관한 문헌적 고찰)

  • 박광희;유화숙
    • Journal of the Korean Home Economics Association
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    • v.41 no.6
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    • pp.167-183
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    • 2003
  • This study reviewed the literature pertaining to the cause and effects of job stress. Many definitions of stress have been offered and the diversity of these definitions has been generated by medical scientists, psychologists, and behavioral scientists. Most approaches dealing with job stress have involved listing of various sources of job stress, moderators, and outcomes. This study is concerned with the selection of variables, the relationships between job stressors and outcomes, and the effects of moderators on these relationships investigated in job stress research. A review of job stress literature presents that various job stressors (e. g., task characteristics, role characteristics, organizational characteristics, career development, and relationships), moderators (e. g., locus of control, type A and B personality, social support, and demographics), and outcomes (e. g., perceived stress, job satisfaction, job commitment, organizational commitment, performance, turnover, and physiological symptoms) were used for a greater understanding of job stress.

Evaluating Applicability of Photochemical Reflectance Index using Airborne-Based Hyperspectral Image: With Shadow Effect and Spectral Bands Characteristics (항공 초분광 영상을 이용한 광화학반사지수 이용 가능성 평가: 그림자 영향 및 대체 밴드를 중심으로)

  • Ryu, Jae-Hyun;Shin, Jung Il;Lee, Chang Suk;Hong, Sungwook;Lee, Yang-Won;Cho, Jaeil
    • Korean Journal of Remote Sensing
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    • v.33 no.5_1
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    • pp.507-519
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    • 2017
  • The applications of NDVI (Normalized Difference Vegetation Index) as a vegetation index has been widely used to understand vegetation biomass and physiological activities. However, NDVI is not suitable way for monitoring vegetation stress because it is less sensitive to change in physiological state than biomass. PRI (Photochemical Reflectance Index) is well developed to present physiological activities of vegetation, particularly high-light-stress condition, and it has been adopted in several satellites to be launched in the future. Thus, the understanding of PRI performance and the development of analysis method will be necessary. This study aims to interpret the characteristics of light-stress-sensitive PRI in shadow areas and to evaluate the PRI calculated by other wavelengths (i.e., 488.9 nm, 553.6 nm, 646.9 nm, and 668.4 nm) instead of 570 nm that used in original PRI. Using airborne-based hyperspectral image, we found that PRI values were increased in shadow detection due to the reduction of high light induced physiological stress. However, the qualities of both PRI and NDVI data were dramatically decreased when the shadow index (SI) exceeded the threshold (SI<25). In addition, the PRI calculated using by 553.6 nm had best correlation with original PRI. This relationship was improved by multiple regression analysis including reflectances of RED and NIR. These results will be helpful to the understanding of physiological meaning on the application of PRI.