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Effects of Cellulolytic Microbes Inoculation During Deep Stacking of Spent Mushroom Substrates on Cellulolytic Enzyme Activity and Nutrients Utilization by Sheep (버섯부산물 퇴적발효 시 섬유소 분해균 접종이 섬유소 분해성 효소 활력과 면양의 영양소 이용성에 미치는 영향)

  • Kim, Y.I.;Jun, S.H.;Yang, S.Y.;Huh, J.W;Kwak, W.S.
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.667-676
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    • 2007
  • This study was conducted to determine effects of cellulolytic microbes inoculation to sawdust-based spent mushroom substrate(SMS) during deepstacking on fermentation parameters, total microbial counts and cellulolytic enzyme activity and to on SMS nutrients utilization by sheep. For sheep metabolism trials, six sheep(ram, average 54.8kg) were fed a Control diet(70% concentrates, 15% rice straw and 15% SMS with no microbial treatment on a dry basis) and a Treatment diet(the same diet including SMS with a microbial treatment) for 2 trials. Spent mushroom substrates with or without a microbial(4 strains including 1 strain of Enterobacter ludwigii, 1 strain of Bacillus cereus and 2 strains of Bacillus subtillis) treatment (1% of SMS on wet basis) were deepstacked for 7 days. The internal temperatures in 1.2 M/T of SMS deepstacks reached to 50±5℃ within 7 days of storage. Total microbial counts remarkably decreased (P<0.05) with a deepstacking process and were not affected(P>0.05) by the microbial treatment. For fibrolytic enzyme activity, CMCase and xylanase activities were decreased(P<0.05) by a deepstacking process. After deepstacking, the microbial treatment showed about 2.5-times higher(P<0.05) for CMCase activity and about 4-times higher(P<0.05) for xylanase activity than those of the Control. Activities of ligninolytic enzymes such as laccase and MnP were not affected by the microbial treatment. The sheep fed the microbially treated SMS diet had a tendency of greater total tract digestibilities of ash(P=0.051), NFE (P=0.071), hemicellulose(P=0.087) and NDF(P=0.096) than those fed the untreated SMS diet. Nitrogen balance of sheep was not affected(P>0.05) by feeding of microbially treated SMS. Accordingly, these results indicate that cellulolytic microbes inoculation during deepstacking of SMS may improve the bio- utilization of SMS by sheep.

Research on Actual Conditions of Fertilizers Applications based on Farmers' Paddy Fields by Regions (수도재배(水稻栽培) 농가(農家)의 지역별(地域別) 시비실태(施肥實態) 조사연구(調査硏究))

  • Lee, C.S.;Hwang, S.W.;Park, J.K.;Kim, M.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.4
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    • pp.315-320
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    • 1986
  • The investigation was conducted to find out amounts and ratios of N, P and K fertilizers applied on rice in 410 farmers' fields. The application ranges of N, P and K fertilizers, respectively, were 15.4-16.3, 7.6-8.0 and 8.2-8.5kg/10a for high yielding varieties, and 13.1-13.8, 7.0-7.1 and 6.4-7.7kg/10a for ordinary varieties. N fertilizer was applied in the ratio of 56% at basal dressing, 34% at tillering, 9% at panicle formation and 1% at heading stage. The amounts of soil amendment application were 179-192kg/10a for silicate, 1,031-1,360kg/10a for compost, 420-540kg/10a for rice straw, and 17.8-25.2 ton/10a for red earth.

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A Survey on Hanwoo Calf Management Prior to Weaning (이유전 합리적인 송아지 사양관리를 위한 한우농가 송아지 사양관리 실태 조사)

  • Yeo, Joon-Mo;Lee, Sung-Hoon;Ki, Kwang-Seok;Hwang, Jin-Ho;Lee, Sung-Sill;Kim, Wan-Young
    • Journal of Animal Science and Technology
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    • v.54 no.2
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    • pp.141-147
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    • 2012
  • The present survey was conducted to provide basic information on Hanwoo calf management. Eight hundred and sixty-two Hanwoo breeding farms from all nine provinces were surveyed via personal interviews. The percentages of farms categorized by herd size were 30.5%, 32.8%, 26.0% and 10.7% for <50 heads, 51-100 heads, 101-200 heads, and >200 heads, respectively. More than 50% of farms offered calf starter at 6-10 days of age, showing that calf starter was offered relatively at an earlier age. Calf starter was replaced every three days by 30.1% of farms. The percentages of farms replacing starter weekly (19.2%) were even higher than those of replacing starter daily (18.8%), suggesting that the frequency of replacing starter needs to be increased to maintain starter freshness and to increase starter intake. About one-third of farms offered forage at 6-10 days of age and 21% of farms offered even at 1-5 days of age although it has been well known that forage does not contain either nutrient density or nutrient profile necessary to stimulate rumen papillae development, especially before weaning. Furthermore, about half of farms used rice straw with calf starter. Water was offered relatively at an earlier age (1-5 days of age) by 55% of farms. Deciding when to wean calves should be based on starter intake rather than age but less than 50% of farms decided weaning age by starter intake. In conclusions, to reduce weaning age of Hanwoo calves by rapid rumen papillae development it is necessary to provide fresh starter and water by increasing frequency of starter replacing and water trough cleaning and not to feed forage before weaning.

Effect of Reduced N Application and Planting Density on Paddy Rice (수도에 대한 질소감량시비 및 재식밀도의 영향)

  • Hong, Ki-Chang;Shim, Jae-Han;Seo, Yong-Tack;Park, Ro-Dong;Kim, Kil-Yong;Shon, Boe-Kyun;Kim, Yong-Woong
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.1
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    • pp.12-21
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    • 1999
  • This study was carried out to improve the cultivation method of paddy rice when the application amount of nitrogen decreased. We investigated the growth and the yield of paddy rice, the changes of nutrients in the soil solution, soil and paddy rice. The results are as follows. The yield at the N4 plot(N $73kg\;ha^{-1}$) was higher than that of control(N $110kg\;ha^{-1}$). N1(N $55kg\;ha^{-1}$), N2(N $55kg\;ha^{-1}$), and N3(N $73kg\;ha^{-1}$). The number of panicle per square meter at the N4 plot was larger than that of control plot. The increase in yield at the N4 plot was due to increased number of panicle where planting density was two times higher compared to the control plot. The availability of nitrogen at the N4 plot also was two times higher than that of control plot. The pH in soil solution was around 7 and did not change largely. The $K^+$ concentration in soil solution at all plots decreased gradually. The $Ca^{2+}$ concentration in soil solutions increased gradually, and decreased at the later stage slightly. The P concentration in soil solution decreased gradually, increased after the heading stage, and then decreased due to decrease of soil temperature. Leaching of $Ca^{2+}$ was higher, while that of $K^+$ and P was lower compared to other ions. Total content of nutrients in soil after harvest decreased. Contents of total N, total P, and O.M. at the N4 plot were higher than those of the other plots. The content of total N and O.M. was correlated to yield. Contents of N, P and K in rice straw and grain at the N4 plot were the highest among all plots. In conclusion, to decrease N application amount in paddy rice cultivation as well as to maintain yield, planting density should be twice of control and the amount of application of N fertilizer should be two-third of control where N fertilizer should be applied mainly at 20 and 10 day before heading.

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Spatial Distribution of Rice Root under Long-term Chemical and Manure Fertilization in Paddy (화학비료 및 희비 장기시용에 따른 벼 뿌리 분포 특성)

  • 전원태;박창영;조영손;박기도;윤을수;강위금;박성태;최진용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.484-489
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    • 2003
  • It is well known that root distribution of rice is a crucial factor for nutrient absorbtion and affect by soil fertility management. However, the findings on root distribution are limited due to laborious and tedious work. The characteristics of root distribution were investigated in long-term fertilizer experiment plots that were established in paddy soil, a fine silty family of typic Hal-paqueps (Pyeongtaeg series) in 1967. fertilizer experiment plots of no fertilizer, compost, NPK and NPK+compost plot have been maintained consistently for the past thirty six year and Npk+silicate plot for the past twenty two years. In NPK plot, 150kg N (urea), 100kg -$\textrm{P}_2\textrm{O}_5$ (fused phosphate) and 100kg $\textrm{K}_2\textrm{O}$(potassium chloride) per hectare have been applied. For NPK+silicate plot, 500kg $\textrm{Si}\textrm{O}_2$ (silicate) was applied in addition to fertilizer in NPK plot. For the compost plot, 10,000kg rice straw compost per hectare were applied. Root samples were taken from the positions of hill-center (below hill) and mid-point of four adjacent rice hills at heading stage by cylinder monolith (CM) method. The soil cores were sampled 20cm depth from the soil surface and partitioned four into layers at an interval of 5cm. The soil particles surrounding roots were washed out with tap water, Length and weight of the roots in each soil layer were measured and root length density (RLD), root weight density (RWD), specific root length(SRL) and rooting depth index (RDI) were calculated. Total root length was measured by intersection method. Plant height, tiller and shoot dry weight were the highest in NPK+compost plot. But RLD of hill-center soil cores was the highest in no-fertilizer plots. In the soil cores from mid-point position of four adjacent hills, RLD at 15-20cm soil depth was higher in compost plot than NPK plot. RLD in compost plots showed even distribution compared to those in chemical- fertilizer plots. RWD was the highest in the NPK+compost plot. SRL was the lowest in the NPK+silicate plot. RDI was the highest in the compost plot. Also, in this experiment it was found that the distribution of roots was closely related to the physical properties of the soil as affected by fertilization management.

Preservation of Strawberries and Cucumbers Packaged by Low density polyethylene film impregnated with antimicmbial agent, Scutellariae baicalensis extract (황금추출물을 함유한 항균성 포장필름을 이용한 딸기와 오이의 저장효과)

  • 정순경;조성환
    • Food Science and Preservation
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    • v.9 no.3
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    • pp.271-276
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    • 2002
  • To develop a wrapping film, which suppresses the microbial decay through the storage and prolongs the selflife of fruits and vegetables, the antimicrobial packaging films were prepared and applied to the preservation of strtwberries and cucumbers. Low density polyethylene(LDPE) film of 50㎛ thickness was faricated with 1% of Scutellariae baicalensis extract. The LDPE film impregnated with Scutellariae baicalensis extract showed antimicrobial activity on the disk test against Bacillus cereus, Escherchia coli and Fusarium sp.. The antimicrobial film changed the color and light transmittance, but did not affect heat shrinkage, mechanical tensile strength and wattability. Strawberries and cucumbers were separately wrapped with packaging films in the state of closely-adhered packaging as well as modified atmosphere packaging(MAP). The wrapped strawberries and cucumbers were stored for 21 days at 5$\^{C}$ and for 40 days at l0$\^{C}$, respectively. For the packaged strawberries and cucumbers at 5$\^{C}$ and 10$\^{C}$, the LDPE film impregnated with Scutellariae baicalensis extract showed the reduced growth of total aerobic bacteria, molds and yeasts and did not give any negative effect on other quality attributes during storage in comparison with conttrol film without any additive.

Liming Effect on Cadmium Immobilization and Phytoavailability in Paddy Soil Affected by Mining Activity (중금속 오염 논토양에서 카드뮴의 부동화와 식물이용성에 대한 석회 시용 효과)

  • Hong, Chang Oh;Kim, Yong Gyun;Lee, Sang Mong;Park, Hyean Cheal;Kim, Keun Ki;Son, Hong Joo;Cho, Jae Hwan;Kim, Pil Joo
    • Korean Journal of Environmental Agriculture
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    • v.32 no.1
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    • pp.1-8
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    • 2013
  • BACKGROUND: Many studies associated with cadmium (Cd) immobilization using lime fertilizer have been conducted for several decades. However, these studies did not suggest exact mechanism of Cd immobilization using lime fertilizer and evaluated effect of lime fertilizer on Cd phytoavailability in rice paddy soil under field condition. METHODS AND RESULTS: This study was conducted to determine exact mechanism of Cd immobilization using lime fertilizer and evaluate liming effect on Cd uptake of rice in contaminated paddy soil. $Ca(OH)_2$ was mixed with Cd contaminated arable soil at rates corresponding to 0, 1,000, 2,000, 4,000, and 8,000 mg/kg. The limed soil was moistened to paddy soil condition, and incubated at $25^{\circ}C$ for 4 weeks. $NH_4OAc$ extractable Cd concentration in soil decreased significantly with increasing $Ca(OH)_2$ rate, since $Ca(OH)_2$ markedly increased net negative charge of soil by pH increase, and decreased bioavailable Cd fractions (F1; exchangeable + acidic and reducible Cd fraction). Calculated solubility diagram indicated that Cd solubility was controlled by soil-Cd. $NH_4OAc$ extractable Cd and F1 concentration were negatively related to soil pH and negative charge. $Ca(OH)_2$ was applied at rates 0, 2, 4, and 8 Mg/ha and then cultivated rice in the paddy soil under field condition. Cadmium concentrations in grain, straw, and root of rice plant decreased significantly with increasing application rate of $Ca(OH)_2$. CONCLUSION(S): Alleviation of Cd phytoavailability with $Ca(OH)_2$ can be attributed primarily to Cd immobilization due to the increase in soil pH and negative charge rather than precipitation of $Cd(OH)_2$ or $CdCO_3$, and therefore, $Ca(OH)_2$ is effective for reducing Cd phytoavailability of rice in paddy soil.

Effects of Salt Treatment on Yield and Physiological Characteristics of Flag Leaf at Heading Stage in Winter Barley (보리 출수기 염처리가 수량 및 지잎의 생리적 특성에 미치는 영향)

  • 최원열;김영민;박종환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.6
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    • pp.409-412
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    • 2002
  • This research was conducted to obtain the fundamental data on salt injury and different responses among cultivars in winter barley (Hordeum vulgare L.). Salts did not affect yield components including number of panicles, stem length, grain number per ear and grain yield while reduced stem dry weight and thousand seed weight significantly with increasing concentrations of salt from 60 to 180 mM. NaCl had less injury effect on barley straw dry weight and thousand seed weight than did $MgSO_4$. Chlorophyll content and relative turgidity in flag leaf were reduced when treated with both salts, while free proline in the salt-treated leaf was increased. Content of proline in salt-treated barley was about 10 folds compared to the control. Based on yield components and physiological traits of flag leaf, the tolerance to salt injury was the greatest in Baegdong, followed by Dongbori#1, Mogpo#55, and Gangbori. The results suggested that salt- stressed barley at reproductive stage had higher free proline content, and that special management in this stage must be considered because salt stress at heading stage affect flag leaf growth as well as yield components Harmfully.

Formaldehyde Monitoring of Hygiene Products in Domestic Market (국내 유통 위생용품 중 포름알데히드 잔류량 모니터링)

  • Na, Young-Ran;Kwon, Hyeon-Jeong;Cho, Hyun-Nho;Kim, Hyeon-Jin;Park, Yon-Koung;Park, Sung-Ah;Lee, Seong-Ju;Kang, Jung-Mi
    • Journal of Food Hygiene and Safety
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    • v.35 no.3
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    • pp.225-233
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    • 2020
  • By the standards and specifications for hygiene products, three test methods for formaldehyde are specified for each item type of hygiene product. After derivatization using acetylacetone and 2,4-dinitrophenylhydrazine (2,4-DNPH), formaldehyde is analyzed by spectrophotometer and high-performance liquid chromatography (HPLC). Validation of the three test methods was performed on tissue, diaper lining and waterproof layer, and panty liner products. The results of linearity (R2), limit of detection (LOD), limit of quantification (LOQ), recovery rate (%) and reproducibility (%), showed that all three methods are suitable for analyzing formaldehyde in hygiene products. After derivatization with 2,4-DNPH and cetylacetone, formaldehyde was analyzed at 0, 3, 6, 9, 24 and 48 hours by HPLC. Formaldehyde derivatized with 2,4-DNPH showed no statistically significant change in formaldehyde peak area over time (P>0.05). But, acetylacetone-derivatizated formaldehyde showed a negative correlation coefficient (r) over time (P<0.01). We investigated the residual amounts of formaldehyde in 205 hygiene products distributed in Busan. Among 74 disposable diaper products tested, 73 had low concentrations of formaldehyde (0.13-29.87 mg/kg). Moreover, formaldehyde was not detected in any of 78 tissue, 27 disposable paper towel, 12 disposable dishcloth, 7 paper cup, one brand of paper straw and 6 disposable napkin products.

Studies on Kimchi for its Standardization for the Industrial Production Part 1. Survey of Status Industrial Production (김치의 공업적(工業的) 생산(生産)을 위한 공업표준화(工業標準化)에 관(關)한 연구(硏究) 제(第)1보(報) 공업적생산(工業的生産)을 위한 조사(調査))

  • Yu, Tai-Jong;Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.6 no.2
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    • pp.116-123
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    • 1974
  • 1) In Seoul, it was known that the season for preparing Kimchies (fermented vegetables) for the winter was from the middle of November to early in December, in which the preparing rates of Kimchies in the middle of November, the end of November and early in December were 32.7%, 41.3% and 12.5%, respectively. The time that the largest quantity of them was prepared was about the end of November. 2) The average cost of Kimchies prepared for the winter for a family of four, five, six and seven was $10,000{\sim}15,000$ Won, $15,100{\sim}20,000$ Won, $10,100{\sim}20,000$ Won and $10,100{\sim}20,000$ Won, respectively, and the cost did not increase in proportion to members of a family. In case of the family of $6{\sim}7$, the cost for Kimchies showed a wide range compared with those for the family of $4{\sim}5$ 3) The main raw materials of kimchi for one person for the winter required $12{\sim}20$ heads of Chinese cabbages, $14{\sim}20$ roots of radishes, $4{\sim}7$ cloves of garlics and $300{\sim}500g$ of powdered red pepper. 4) The residents living in Seoul had prepared the several kinds of the pickles for the winter as follows; (1) Chinese cabbage Kimchi (98.9%), (2) Whole Chinese cabbage Kimchi (74.7%), (3) Kkakdugi (68.6%), (4) Dongchimi (66.4%) and (5) Chong-kak Kimchi (63.3%). It has, therefore. been considered that the five kinds of Kimchies mentioned above may be industrialized. 5) Uniqueness of the raw materials used for the most popular Chinese cabbage Kimchi was to use leeks, garlics, red peppers and gingers as spices, and it was also known that proper amounts of salted shrimp pickles and oysters was mixed to the Kimchies. Therefore, it had been considered that the characteristics of Chinese cabbage Kimchies for the winter had the hot taste with freshness. 6) For keeping the Kimchies during the winter about the half of the pickle jars was buried in the ground, and another half of them were wrapped in the straw bags or styropol and they were placed on the ground or kept in the basement, 7) In most case (80.9%), the salt concentration of pickling was adjusted by one's experiences, and only 19% of them was measured with the instruments. 8) Most of remaining kimchies were usually used for other cooks, but some of them were thrown away. 9) The ratio of the people who had ever bought the market Kimchies for their own edibility was 17.8% and most of them got it only in the spring and summer season. 10) About 18% of the residents living in the general houses in Seoul had ever bought the market Kimchies. It was also known that about 48% of the residents living in the general houses and about 79% of the residents living in the apartments wanted to purchase the market Kimchies if the production of the delicious Kimchies were industrialized. The season that the people wanted to get the market Kimchies was a little different each other among the residents. About 13.4% of the residents living in the general houses wanted to purchase the Kimchies during the summer, and 11.9% of them wanted to get it throughout the year. On the other hand, in case of the apartments, 25.2% of the residents wanted to get it during the summer and 24.4% of them wanted it throughout the year (4 seasons) and 19.9% of them wanted it during two seasons. The data mentioned above have shown that many residents hope strongly an industrial production of the Kimchies. It is also true that many residents living in both the general houses and apartments want to get the market kimchies throughout the year, and particulary during the summer season that most foods are very apt to be spoiled.

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