• Title/Summary/Keyword: Strain recovery

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Artificial rearing of the olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) for use in the Sterile Insect Technique: improvements of the egg collection system

  • Ahmad, Sohel;Haq, Ihsan ul;Rempoulakis, Polychronis;Orozco, Dina;Jessup, Andrew;Caceres, Carlos;Paulus, Hannes;Vreysen, Marc J.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.1
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    • pp.15-23
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    • 2016
  • One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.

On the Properties and Intersection Feature of the Ductile Shear Zone (Chonju shear zone) near Yongkwang-Eub (영광(靈光) 부근(附近) 연성전단대(延性剪斷帶)(전주전단대(全州剪斷帶))의 성질(性質)과 교차양상(交叉樣相)에 관(關)하여)

  • Jeon, Kyeong Seok;Chang, Tae Woo;Lee, Byung Joo
    • Economic and Environmental Geology
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    • v.24 no.4
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    • pp.435-446
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    • 1991
  • Ductile shear zones developed in Jurassic granites in the Yonggwang area show NE trend at the eastern part and nearly EW trend at the western part, respectively. Judged from shear sense indicators, they have resulted from dextral strike-slip movement. The intersection of both trends is thought to be due to the truncation and offset of NE shear zone Chonju Shear zone by the brittle Yonggwang fault which runs in near EW direction with sinistral movement sense. The simple shear deformation was predominate through the deformation in this ductile shear zone. Based on this deformation mechanism, the shear strain (${\gamma}$) estimated in domain 1 increases from 0.14 at the shear zone margin to 9.41 toward the center of shear zone. Total displacement obtained only from this measured section(JK 59 to JK14) appecars to be 1434.5 meters. The sequential development of microstructures can be divided into three stages; weakly-foliated, well-foliated and banded-foliated stages. In the weakly-foliated stage dislocation glide mechanism might be predominant. In the well-foliated stage grain boundary migration and progressive misorientation of subgrains was remarkable during dynamic recovery and recrystallization. In the banded-foliated stage grain boundary sliding and microfracturing mechanisms accompanied with crushing and cracking were marked. According to strain analysis from quartzites of the metasedimentary rocks, strain intensity (${\gamma}$) of the samples within the ductile shear zone ranges from 2.7 to 5.7, while that of the samples out of the ductile shear zone appears to be about 1.7.

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Precise Flow Stress Analysis for the Occurrence of Dynamic Ferritic Transformation and Dynamic Recrystallization of Austenite in Low Carbon Steel (고온 변형 곡선을 이용한 동적 재결정 해석과 동적 상변태의 조기 예측)

  • Park, Nokeun
    • Korean Journal of Metals and Materials
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    • v.56 no.11
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    • pp.779-786
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    • 2018
  • There have been previous attempts to observe the occurrence of dynamic ferritic transformation at temperatures even above $Ae_3$ in a low-carbon steel, and not only in steels, but recently also in titanium alloys. In this study, a new approach is proposed that involves treating true stress-true strain curves in uniaxial compression tests at various temperatures, and different strain rates in 0.1C-6Ni steel, which is a model alloy used to decelerate the kinetics of ferrite transformation from austenite. The initial flow stress up to peak stress was used to analyze the change in dynamic softening phenomena, such as dynamic recovery, dynamic recrystallization, and dynamic transformation. It is worth mentioning that for predicting the occurrence of dynamic transformation, flow stress before reaching peak stress is much more sensitive to the change in the dynamic softening rate due to dynamic transformation, compared to peak stress. It was found that the occurrence of dynamic ferritic transformation could be successfully obtained even at temperatures above $Ae_3$ once the deformation condition was satisfied. This deformation condition is a function of both the strain rate and the deformation temperature, which can be described as the Zener - Hollomon parameter. In addition, the driving force of dynamic ferritic transformation might be much less than that of the dynamic recrystallization of austenite at a given deformation condition. By applying this technique, it is possible to predict the occurrence of dynamic transformation more sensitively compared with the previous analysis method using peak stress during deformation.

Load Recovery Using D-Optimal Sensor Placement and Full-Field Expansion Method (D-최적 실험 설계 기반 최적 센서 배치 및 모델 확장 기법을 이용한 하중 추정)

  • Seong-Ju Byun;Seung-Jae Lee;Seung-Hwan Boo
    • Journal of the Society of Naval Architects of Korea
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    • v.61 no.2
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    • pp.115-124
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    • 2024
  • To detect and prevent structural damage caused by various loads on marine structures and ships, structural health monitoring procedure is essential. Estimating loads acting on the structures which are measured by sensors that are mounted properly are crucial for structural health monitoring. However, attaching an excessive number of sensors to the structure without consideration can be inefficient due to the high costs involved and the potential for inducing structural instability. In this study, we introduce a method to determine the optimal number of sensors and their optimized locations for strain measurement sensors, allowing for accurate load estimation throughout the structure using model expansion method. To estimate the loads exerted on the entire structure with minimal sensors, we construct a strain-load interpolation matrix using the strain mode shapes of the finite element (FE) model and select the optimal sensor locations by applying D-Optimal Design and the row exchange algorithm. Finally, we estimate the loads exerted on the entire structure using the model expansion method. To validate the proposed method, we compare the results obtained by applying the optimal sensor placement and model expansion method to an FE model subjected to arbitrary loads with the loads exerted on the entire FE model, demonstrating efficiency and accuracy.

Mechanical and Operational Factors Affecting the Efficiency of Rice Polishing Machines (정미기의 능률에 미치는 기계적 요인및 작동조건에 관한 연구)

  • No, Sang-Ha
    • Journal of Biosystems Engineering
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    • v.1 no.1
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    • pp.15-15
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    • 1976
  • In analyzing the operational characteristics of a rice whitening machine, the internal radial pressure of the machine was measured using strain gage equipment. Changes in cylinder and feed screw configurations, screen type, cylinder speed and counter-pressure levels were examined to determine their impact on the quality and quantity of milled rice and the performance of the machine. The results are summarized as follows: 1. The internal radial pressure in the whitening chamber varied with the surface condition of the grain being processed. During the first or second pass through the machine, pressure was relatively low, reached a maximum after two to three passes with combinations I and II, three to six with combination III and then began to fall. 2. The pitch of the feed screw and the size of the feed gate opening which determine the rate of entry of grain into the whitening chamber, appeared to be the most important factor aff-::cting the degree of radial pressure, quality and quantity of milled rice and the efficiency of the machine. Using a feed screw with a wide pitch (4.8cm), radial pressure was relatively high and head rice recovery ratio \vere quite low. In this case capacity and machine effic?iency were much higher than obtained when using a feed screw with a narrow pitch (2.3cm). Very significant responses in radial pressure, head rice recovery rates and machine capacity were observed with changes in cylinder speed and counter-pressure levels when using the wide pitch feed screw. 3. The characteristics of the screen which surrounds the whitening chamber had an important effect on whitening efficiency. The existence of small protuberances on the original screen resulted in significant increases in both machine capacity and efficiency but without a significant decrease in head rice recovery or development of excessive radial pressure. Further work is required to determine the effects of screen surface conditions and the shape of the cylinderical steel roller on the rate of bran removal, machine efficiency and recovery rates. The size of the slotted perforations 0:1 the screen affects total milled rice recovery. The opening size on the original screen was fabricated to accommodate the round shape of Japonica rice varieties but was not suitable for the more slender Indica type. Milling Indica varieties with this screen resulted in a reduction in total milled rice recovery. 4. An increase in cylinder speed from 380 to 820 rpm produced a positive effect on head rice recovery for all machine combinations at every level of counter-pressure used in the tests. Head rice recovery was considerably lower at 380rpm using a wide screw pitch when compared to the results obtained at speeds from 600 to 820 r.p.m. The effects of cylinder speed On radial pressure, capacity and machine efficiency showed contrasting results, depending on the width of the feed screw pitch. With a narrow feed screw pitch (2.3cm), a direct proportional relationship was observed bet?ween cylinder speed and both radial pressure and machine efficiency. In contrast, using a 4.8 centimeter pitch feed roller produced a series of inverse relationships between the above variables. Based on the results of this study it is recommended when milling Indica type long grain rice varieties that the cylinder speed of the original machine be increased from 500-600 rmp up to a minimum of 800 rpm to obtain a greater abrasive effect between the grain and the screen. The pitch of the feed screw should be also reduced to decr?ease the level of internal radial pressure and to obtain higher machine efficiency and increased quality of milled rice with increased cylinder speeds. Further study on the interaction between cylinder speed and feed screw pitch is recommended. 5. An increase in the counter pressure level produced a negative effect On the head rice recovery with an increase in radial pressure, capacity, and machine efficiency over all combinations and at every level of cylinder speed. 6. Head rice recovery rates were conditioned primarily by the pressure inside the whitening chamber. According to the empirical cha racteristics curve developed in this study, the relationships of head rice recovery ($Y_h$) and machine capacity ($Y_c$/TEX>) to internal radial pressure ($X_p$) followed an inverse quadratic function and a linear function respectively: $$Y_h^\Delta=\frac{1}{{1.4383-0.2951X_p^\ast+0.1425X_p^{\ast\ast}}^2} , (R^2=0.98)$$$$Y_c^\Delta=-305.83+374.37X_p^{\ast\ast}, (R^2=0.88)$$The correlation between capacity and power consumption per unit of brown rice expressed in the following exponential function: $$Y_c^\Delta=1.63Y_c^{-0.7786^\{\ast\ast}, (R^2=0.94)$$These relationships indicate that when radial pressure increases above a certain range (1. 6 to 2.0 kg/$cm^2$ based On the results of the experiment) head ricerecovery decrea?ses in a quadratic relation with a inear increase in capacity but without any decrease in power consump tion per unit of brown rice. On the other hand, if radial pressure is below the range shown above, power consumption increases dramatically with a lin?ear decrease in capacity but without significant increases in head rice recovery. During the operation of a given whitening machine, the optimum radial pressure range or the correct capacity range should be selected by controlling the feed rate and/or counter-pressure keeping in mind the condition of the grain, particulary the hardness. It was observed that the total number of passes is related to radial pessure level, feed rate and counter-pressure level. The higher theradial pressure the fewer num?ber of pass required but with decreased head rice recovery. In particular, when using high feed rates, the total number of passes should be increased to more than three by reducing the counter-pressure level to avoid decreaseases in head rice recovery (less than 65 percent head rice recovery on the basis of brown rice) at every cylinder speed. 7. A rapid rise in grain temperature seemed to have a close relationship with the pressure generated inside the whitening chamber and, subsequently with head rice reco?very rates. The higher the rate of increase, the lower were the resulting head rice recoveries.

Mechanical and Operational Factors Affecting the Efficiency of Rice Polishing Machines (정미기의 능률에 미치는 기계적 요인및 작동조건에 관한 연구)

  • 노상하;최재갑
    • Journal of Biosystems Engineering
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    • v.1 no.1
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    • pp.17-48
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    • 1976
  • In analyzing the operational characteristics of a rice whitening machine, the internal radial pressure of the machine was measured using strain gage equipment. Changes in cylinder and feed screw configurations, screen type, cylinder speed and counter-pressure levels were examined to determine their impact on the quality and quantity of milled rice and the performance of the machine. The results are summarized as follows: 1. The internal radial pressure in the whitening chamber varied with the surface condition of the grain being processed. During the first or second pass through the machine, pressure was relatively low, reached a maximum after two to three passes with combinations I and II, three to six with combination III and then began to fall. 2. The pitch of the feed screw and the size of the feed gate opening which determine the rate of entry of grain into the whitening chamber, appeared to be the most important factor aff-::cting the degree of radial pressure, quality and quantity of milled rice and the efficiency of the machine. Using a feed screw with a wide pitch (4.8cm), radial pressure was relatively high and head rice recovery ratio \vere quite low. In this case capacity and machine effic\ulcorneriency were much higher than obtained when using a feed screw with a narrow pitch (2.3cm). Very significant responses in radial pressure, head rice recovery rates and machine capacity were observed with changes in cylinder speed and counter-pressure levels when using the wide pitch feed screw. 3. The characteristics of the screen which surrounds the whitening chamber had an important effect on whitening efficiency. The existence of small protuberances on the original screen resulted in significant increases in both machine capacity and efficiency but without a significant decrease in head rice recovery or development of excessive radial pressure. Further work is required to determine the effects of screen surface conditions and the shape of the cylinderical steel roller on the rate of bran removal, machine efficiency and recovery rates. The size of the slotted perforations 0:1 the screen affects total milled rice recovery. The opening size on the original screen was fabricated to accommodate the round shape of Japonica rice varieties but was not suitable for the more slender Indica type. Milling Indica varieties with this screen resulted in a reduction in total milled rice recovery. 4. An increase in cylinder speed from 380 to 820 rpm produced a positive effect on head rice recovery for all machine combinations at every level of counter-pressure used in the tests. Head rice recovery was considerably lower at 380rpm using a wide screw pitch when compared to the results obtained at speeds from 600 to 820 r.p.m. The effects of cylinder speed On radial pressure, capacity and machine efficiency showed contrasting results, depending on the width of the feed screw pitch. With a narrow feed screw pitch (2.3cm), a direct proportional relationship was observed bet\ulcornerween cylinder speed and both radial pressure and machine efficiency. In contrast, using a 4.8 centimeter pitch feed roller produced a series of inverse relationships between the above variables. Based on the results of this study it is recommended when milling Indica type long grain rice varieties that the cylinder speed of the original machine be increased from 500-600 rmp up to a minimum of 800 rpm to obtain a greater abrasive effect between the grain and the screen. The pitch of the feed screw should be also reduced to decr\ulcornerease the level of internal radial pressure and to obtain higher machine efficiency and increased quality of milled rice with increased cylinder speeds. Further study on the interaction between cylinder speed and feed screw pitch is recommended. 5. An increase in the counter pressure level produced a negative effect On the head rice recovery with an increase in radial pressure, capacity, and machine efficiency over all combinations and at every level of cylinder speed. 6. Head rice recovery rates were conditioned primarily by the pressure inside the whitening chamber. According to the empirical cha racteristics curve developed in this study, the relationships of head rice recovery ($Y_h$) and machine capacity ($Y_c$/TEX>) to internal radial pressure ($X_p$) followed an inverse quadratic function and a linear function respectively: $$Y_h^\Delta=\frac{1}{{1.4383-0.2951X_p^\ast+0.1425X_p^{\ast\ast}}^2} , (R^2=0.98)$$ $$Y_c^\Delta=-305.83+374.37X_p^{\ast\ast}, (R^2=0.88)$$ The correlation between capacity and power consumption per unit of brown rice expressed in the following exponential function: $$Y_c^\Delta=1.63Y_c^{-0.7786^\{\ast\ast}, (R^2=0.94)$$ These relationships indicate that when radial pressure increases above a certain range (1. 6 to 2.0 kg/$cm^2$ based On the results of the experiment) head ricerecovery decrea\ulcornerses in a quadratic relation with a inear increase in capacity but without any decrease in power consump tion per unit of brown rice. On the other hand, if radial pressure is below the range shown above, power consumption increases dramatically with a lin\ulcornerear decrease in capacity but without significant increases in head rice recovery. During the operation of a given whitening machine, the optimum radial pressure range or the correct capacity range should be selected by controlling the feed rate and/or counter-pressure keeping in mind the condition of the grain, particulary the hardness. It was observed that the total number of passes is related to radial pessure level, feed rate and counter-pressure level. The higher theradial pressure the fewer num\ulcornerber of pass required but with decreased head rice recovery. In particular, when using high feed rates, the total number of passes should be increased to more than three by reducing the counter-pressure level to avoid decreaseases in head rice recovery (less than 65 percent head rice recovery on the basis of brown rice) at every cylinder speed. 7. A rapid rise in grain temperature seemed to have a close relationship with the pressure generated inside the whitening chamber and, subsequently with head rice reco\ulcornervery rates. The higher the rate of increase, the lower were the resulting head rice recoveries.

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Oocyst production and immunogenicity of Cryptosporidium muris (strain MCR) in mice (마우스에 있어서 쥐와포자충(MCR주)의 오오시스트 배설상황과 면역원성)

  • Lee, Jae-Gu;Yok, Sim-Yong;Park, Bae-Geun
    • Parasites, Hosts and Diseases
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    • v.33 no.4
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    • pp.377-382
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    • 1995
  • Three-week-old ICR SPF mice were orally inoculated with one of 5 doses ranging from $2{\;}\times{\;}10^2{\;}to{\;}2{\;}\times{\;}10^6$ oocysts of Crwptosporidium tsuris (strain MCR) per mouse. Oocyst inoculation was directly proportional to the amount of oocysts shed and was inversely proportional to the period required for peals oocyst production and to the prepatent period. Peak oocyst production occurred between fifteen and thirty-one days with a patent period from 61 to 64 days. Three days after all mice stopped shedding oocysts, they were orally challenged with a single dose of $2{\;}\times{\;}10^6$ oocysts or the same species. Marked seroconversion for IgG antibody accompanied recovery from mice inoculated with $5{\;}\times{\;}10^5$ oocysts. Mice administered with carrageenan excreted a small number of oocysts for 49.0 days on the average after challenge inoculation (ACI) and control mice for 14.2 days in a dose-independent fashion. Just before challenge infection, phagocytic activity of peritoneal macrophages ($M{\phi}$) and the number of peripheral $M{\phi}$ were dramatically decreased. Mild challenge infection implies that the immunogenicity of C. nuris (strain MCR) is very strong, despite $M{\phi}$ blocker carrageenan administration.

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Agronomic Characteristics and Aromatic Compositions of Korean Wild Codonopsis lanceolata Collections Cultivated in Field (한국 야생더덕 수집종의 노지 재배시 생육 특성과 향기성분 조성)

  • 이승필;김상국;민기군;조지형;최부술;이상철;김길웅
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.2
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    • pp.188-199
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    • 1996
  • The native ecological environment and aromatic constituents of Korean wild Codonopsis lanceolata and one Japanese strain were investigated to find Codonopsis lanceolata strains showing high aromatics, and to know regional differences among these strains. The results were as follows : There were no remarkable differences among the Korean wild C. lanceolata strains in ecological environments. Recovery yield of essential oils was highest in Togyusan strain with 0.009%. Difference in protein band patterns among these strains was not recognized, and peroxidase and esterase pattern changes were appeared in different collected regions at the leaf and root tissues. Major aromatic constituents were 11 kinds of aliphatic alcohols such as trans-2-hexenal, 1-hexanol, cis-3-hexanol, and trans-2-hexanol. And Togyusan strain, Sobaeksan strain, and Kayasan strain have the highest aliphatic alcohols of plant essential oils. In particular, BHT(butylated hydroxytoluene), one of the antioxidants, was detected in Chirisan strains.

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Effect of the amino acid mixture on freeze-drying and preservation of Lactobacillns casei YIT 9018 (아미노산 혼합용액이 Lactobacillus casei YIT 9018의 동결건조 및 저장성에 미치는 영향)

  • 윤성식;이해옥;유주현
    • Microbiology and Biotechnology Letters
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    • v.14 no.5
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    • pp.421-426
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    • 1986
  • This study was attempted to find out effective storage methods of Lactobacillus casei YIT 9018, industrial strain for fermented mil k production, without severe bacterial death and activity deteriorations. The cryoprotective effect of the ammo acid mixture consisting of glycine and DL-g1utamic acid on the test strain were examined and also compared with those other protectants already reported. The apparent protective effect by the amino acid mixture was observed to controls. Both glycine and DL-glutamic acid prevented the freezing death of test strain and his effect of 1. casei YIT 9018 had reached stationary stage in MRS-broth 18h after inoculation. Cells harvested from stationary stage were most resistant to freezing damage. The viability of the test strain was affected by rehydration media and the recovery of viable cells was increased about threefold when amino acid mixture was used for rehydration. The presence of non-fat milk solid (NFMS), sucrose and lactose in amino acid mixture increased viability of the test strain up to 85%. In this case, optimal concentrations of NFMS, sucrose and lactose were 10%, 7.5-10%, 7.5-10%, respectively.

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Lysine Production by Thialysine Resistant Mutant of Candida utilis ( I ) - Isolation of High Lysine Excreting Mutant of Candida utilis - (Candida utilis의 Thialysine 내성맥리주에 의한 Lysine생산 ( I ) -Candida utilis의 Lysine을 생산하는 Thialysine 내성맥리주의 분리-)

  • Bang, Byung-Ho;Seu, Jung-Hwn
    • Microbiology and Biotechnology Letters
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    • v.11 no.3
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    • pp.175-180
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    • 1983
  • Thialysine significantly inhibited the growth of wild type strain Gondida utilis NCYC-359. In the absence of thialysine, the culture reached stationary phase after 24hr, however, in the presence of 0.5% thialysine, the culture reached stationary phase after 40hr, respectively. Effect of amino acid or vitamin was investigated on recovery of the growth of wild type strain from thialysine inhibition. Glycine, methionine, arginine and tryptophan recovered growth inhibition by thialyslne to some extent. However, vitamins were inert. Especially, lysine at one eighth concentration of thialysine recovered almost fully the growth inhibition. Thialysine resistant mutants were induced from the parent strain of Condida utilis NCYC-359 by NTG treatment. Colonies of thialysine resistant mutants were obtained on agar minimal medium supplemented with 0.1-0.5% thialysine. The frequency of thialysine resistant mutants induced by the first mutation was the highest at 0.1% The wild strain produced no appreciable lysine extracellularly. However, almost thialysine resistant mutants excreted appreciably. Lysine excretion increased after repeated mutation. Finally, of the thialysine resistant mutants induced by NTG, Condida utilis TRN-4006 was obtained. This strain excreted lysine (400$\mu\textrm{g}$/$m\ell$) into the medium with a concomitant decrease of lysine in the intracellular pool.

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