• Journal of the Earthquake Engineering Society of Korea
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• v.9 no.6 s.46
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• pp.59-65
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• 2005

In this paper a constitutive model for large cracks in concrete and other brittle materials subject to dynamic and cyclic leading is presented. The suggested model is based on the plastic-damage model for cyclic leading. A numerical formulation based on the three-step return-mapping algorithm for the proposed large crack model is also present. The numerical examples show that the present algorithm works appropriately under dynamic leading and should be used in large crack problems to prevent excessive tensive plastic strain development causing unrealistic results.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2002.05a
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• pp.125-128
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• 2002

The automotive industry have made an effort to reduce the weight of vehicle structures with increased safety, while initial model of the final product does not contain any prehistoric effects in a design stave. It takes lots of time to calculate forming effects that have great influences on the energy absorption of structures. In this paper, finite element inverse analysis is adopted to calculate forming effects, such as thickness variation and effective plastic strain as well as an initial blank shape with small amount of computation time. Crash analysis can be directly performed after inverse analysis of the forming process without remeshing scheme. The direct mesh mapping method is used to calculate an initial guess from the sliding constraint surface that is extracted from the die and punch set. Analysis results show that energy absorption of structures is increased with consideration of forming effects and finite element inverse analysis is usefully applicable to calculate forming erects of vehicle structures for the crash analysis.

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.151-162
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• 1996

Pseudorabies is caused by Pseudorabies virus (PRV: Aujeszky's disease virus) of Herpesviridae that is characterized by 100 to 150nm in size with a linear double-stranded DNA molecule with of approximately $90{\times}10^6Da$. This disease affects most of domestic animals such as swine, cattle, dog, sheep, cat, chicken, etc. causing high mortality and economic losses. In swine, young piglets show high mortality and pregnant sows, reproductive failures. However the adult swine reveals no clinical signs in general. But they become a carrier state and play an important role for propagation of the disease. In this study, the nucleotide sequence of major casid protein gene of PRV, Yangsan strain isolated from the diseased swine in Korea was analyzed, and the recombinant MCP was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. As result, in BamHI digestion, MCP gene locus of PRV YS strain showed different from that of Indiana S strain. The patterns of enzyme mapping were also found to be unidentical each other. The sequence of the MCP gene partially analyzed showed 98.09% identity to Indiana S strain. The expression of MCP in Sf-9 cell cotransfected by pVLMCP-44 baculovirus expression vector was characterized by Southern blot hybridization, immunofluoresent and immunocytochemical tests, SDS-PAGE and Western blotting. The rMCP with M.W. 142kDa was most effectively expressed in Sf-9 cells at the 3-4th days post inoculation of the recombinant baculovirus by 2 moi.

• Journal of the Korean Society of Combustion
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• v.18 no.4
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• pp.44-52
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• 2013

Important role of chemical interaction in flame extinction was numerically investigated in downstream interaction among lean(rich) and lean(rich) premixed as well as partially premixed $H_2$-air and CO-air flames. The strain rate varied from 30 to $5917s^{-1}$ until interacting flame could not be sustained anymore. Flame stability diagrams mapping lower and upper limit fuel concentrations for flame extinction as a function of strain rate are presented. Highly stretched interacting flames were survived only within two islands in the flame stability map where partially premixed mixture consisted of rich $H_2$-air flame, extremely lean CO-air flame, and a diffusion flame. Further increase in strain rate finally converges to two points. Appreciable amount of hydrogen in the side of lean $H_2$-air flame also oxidized the CO penetrated from CO-air flame, and this reduced flame speed of the $H_2$-air flame, leading to flame extinction. At extremely high strain rates, interacting flames were survived only by a partially premixed flame such that it consisted of a very rich $H_2$-air flame, an extremely lean CO-air flame, and a diffusion flame. In such a situation, both the weaker $H_2$-air and CO-air flames were parasite on the stronger diffusion flame such that it could lead to flame extinction in the situation of weakening the stronger diffusion flame. Particular concerns are focused on important role of chemical interaction in flame extinction was also discussed in detail.

• Korean Journal of Microbiology
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• v.24 no.4
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• pp.357-363
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• 1986

Synthesis of threonine and methionine in yeast, Saccharomyces cerevisiae shares a common pathway from aspartate via homoserine. HOM6 gene encodes homoserine dehydrogenase (HSDH) which catalyzes the inter-conversion of beta-aspartate semialdehyde and homoserine. The level of HSDH is under methionine specific control. A recombinant plasmid (pEK1: 13.3kb), containing HOM6 gene, has been isolated and cloned into E. coli by complenemtary transformation of a homoserine auxotrophic yeast strain M-20-20D (hom6, trp1, ura3) to a prototrophic M20-20D/pEK1, using a library of yeast genomic DNA fragments in a yeast centromeric plasmid, YCp50(8.0kb). Isolation of HOM6has been primarily confirmed by retransformation of the original yeast strain M20-20D, using the recombinant plasmid DNA which was extracted from M20-20D/pEK1 and subsequently amplified in E. coli. Eleven cleavage sites in the insery (5.3kb) have been localized through fragment analysis for 8 restriction endonucleases; Bgl II(2 site), Bgl II(1), Cla I(3), Eco RI(1), Hind III(2), Kpn I (1), Pvu II(1) and Xho I(1).

• Advances in aircraft and spacecraft science
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• v.1 no.2
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• pp.125-143
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• 2014

This paper provides a new technique for solving the static analysis of arbitrarily shaped composite plates by using Strong Formulation Finite Element Method (SFEM). Several papers in literature by the authors have presented the proposed technique as an extension of the classic Generalized Differential Quadrature (GDQ) procedure. The present methodology joins the high accuracy of the strong formulation with the versatility of the well-known Finite Element Method (FEM). The continuity conditions among the elements is carried out by the compatibility or continuity conditions. The mapping technique is used to transform both the governing differential equations and the compatibility conditions between two adjacent sub-domains into the regular master element in the computational space. The numerical implementation of the global algebraic system obtained by the technique at issue is easy and straightforward. The main novelty of this paper is the application of the stress and strain recovery once the displacement parameters are evaluated. Computer investigations concerning a large number of composite plates have been carried out. SFEM results are compared with those presented in literature and a perfect agreement is observed.

• Steel and Composite Structures
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• v.47 no.2
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• pp.289-296
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• 2023

The failure of a thin-walled tube was studied in this paper based on three failure models. Both proportional and non-proportional loading paths were applied. Proportional loading consisted of combined tension-torsion. Cyclic non-proportional loading was also applied. It was a circular out-of-phase axial-shear stress loading path. The third loading path was a combination of a constant internal pressure and a bending moment. The failure models under study were equivalent plastic strain, modified Mohr-Coulomb (Bai-Wierzbicki) and Tearing parameter models. The elasto-plastic analysis was conducted using J2 criterion and nonlinear kinematic hardening. The return mapping algorithm was employed to numerically solve the plastic flow relations. The effects of the hydrostatic stress on the plastic flow and the stress triaxiality parameter on the failure were discussed. Each failure model under study was utilized to predict failure. The failure loads obtained from each model were compared with each other. The equivalent plastic strain model was independent from the stress triaxiality parameter, and it predicted the highest failure load in the bending problem. The modified Mohr-Coulomb failure model predicted the lowest failure load for the range of the stress triaxiality parameter and Lode's angle.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.99-99
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• 2013

In this talk, I will present two research works in progress, which are: i) mapping of piezoelectric polarization and associated charge density distribution in the heteroepitaxial InGaN/GaN multi-quantum well (MQW) structure of a light emitting diode (LED) by using inline electron holography and ii) in-situ observation of the polarization switching process of an ferroelectric Pb(Zr1-x,Tix)O3 (PZT) thin film capacitor under an applied electric field in transmission electron microscope (TEM). In the first part, I will show that strain as well as total charge density distributions can be mapped quantitatively across all the functional layers constituting a LED, including n-type GaN, InGaN/GaN MQWs, and p-type GaN with sub-nm spatial resolution (~0.8 nm) by using inline electron holography. The experimentally obtained strain maps were verified by comparison with finite element method simulations and confirmed that not only InGaN QWs (2.5 nm in thickness) but also GaN QBs (10 nm in thickness) in the MQW structure are strained complementary to accommodate the lattice misfit strain. Because of this complementary strain of GaN QBs, the strain gradient and also (piezoelectric) polarization gradient across the MQW changes more steeply than expected, resulting in more polarization charge density at the MQW interfaces than the typically expected value from the spontaneous polarization mismatch alone. By quantitative and comparative analysis of the total charge density map with the polarization charge map, we can clarify what extent of the polarization charges are compensated by the electrons supplied from the n-doped GaN QBs. Comparison with the simulated energy band diagrams with various screening parameters show that only 60% of the net polarization charges are compensated by the electrons from the GaN QBs, which results in the internal field of ~2.0 MV cm-1 across each pair of GaN/InGaN of the MQW structure. In the second part of my talk, I will present in-situ observations of the polarization switching process of a planar Ni/PZT/SrRuO3 capacitor using TEM. We observed the preferential, but asymmetric, nucleation and forward growth of switched c-domains at the PZT/electrode interfaces arising from the built-in electric field beneath each interface. The subsequent sideways growth was inhibited by the depolarization field due to the imperfect charge compensation at the counter electrode and preexisting a-domain walls, leading to asymmetric switching. It was found that the preexisting a-domains split into fine a- and c-domains constituting a $90^{\circ}$ stripe domain pattern during the $180^{\circ}$ polarization switching process, revealing that these domains also actively participated in the out-of-plane polarization switching. The real-time observations uncovered the origin of the switching asymmetry and further clarified the importance of charged domain walls and the interfaces with electrodes in the ferroelectric switching processes.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.05a
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• pp.241-245
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• 2007

The residual stress in axial stress in the axial direction of the steel filaments has been measured by using a method based on the combination of the focused ion beam (FIB) and high resolution strain mapping program (VIC-2D). That is, the residual stress was calculated from the measured displacement field before and after the introduction of a slot along the steel filaments. The displacement was obtained by the digital correlation analysis of high-resolution scanning electron micrographs, while the slot was introduced by FIB milling with low energy beam. The present measurement revealed that the residual stress within 8% of the magnitude was persistent in the steel filaments fabricated.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.10a
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• pp.134-137
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• 2008

The effects of residual stress and surface defects on the mechanical properties of the high carbon steel filament used for the automotive tire have been experimentally investigated. The samples were fabricated with annealing temperature. The residual stress was measured by focused ion beam and strain mapping software which has advantages, such as data with high accuracy and fast data acquisition time. Mechanical properties, such as tensile strength and fatigue resistance, were gradually increased up to $200^{\circ}C$ and then slightly decreased. From the measurement of residual stress and level of surface defect, it was revealed that the critical factor was varied with different temperature region. That is, the fatigue resistance increased due to decreasing the residual stress and decreased due to increasing the size and distribution of surface defect.