• Title/Summary/Keyword: Strain identification

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Identification of a Strain of Babesia Isolated from Korean Cattle (한우(韓牛)로부터 분리(分離)한 바베시아 원충(原蟲)의 동정(同定))

  • Jeon, Yeong
    • Korean Journal of Veterinary Research
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    • v.18 no.1
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    • pp.27-31
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    • 1978
  • In order to identify unknown Babesia spp. which was isolated from Korean cattle, the morphology of Korean strain was compared with that of Babesia bigemina (Kochinda strain) and Babesia spp. (Miyake strain). Immunofluorescent technique was used to identify the serological character of the parasites. The results obtained were summarized as follows: 1. Korean strain was morphologically very similar to Babesia spp.(Miyake strain) which mostly showed parallel-bigeminate forms, while B. bigemina (Kochinda strain) was mostly round and oval forms. 2. By the indirect fluorescent antibody technique: a) Anti-Babesia spp. and Korean Babesia spp. sera showed a higher antibody titers with Babesia spp. (Miyake strain) antigen (1:500) than with B. bigemina (Kochinda strain) antigen (1:50). b) Anti-Babesia bigemina sera showed a lower titer with Babesia spp. antigen (1:50) than with B. bigemina antigen (1:250). 3. On the basis of morphological and serological confirmantions, a Babesia strain isolated from a Korean cattle was very similar, if not identical, to Miyake strain of Babesia spp.

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Bacteriological Characteristics of Unidentified Vibrio sp., Hemolysin Producer Isolates front Brackish Water -1. Bacteriological Characteristics of Vibrio sp., D9 (V. kumkang) Similar to V. mimicus (기수에서 분리된 용혈독소를 생산하는 미분류 Vibrio sp.의 세균학적 특징 -1. V. mimicus와 유사한 Vibrio sp. D9의 세균학적 특성)

  • KIM Young-Man;OH Hee-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.6
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    • pp.585-590
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    • 2000
  • A hemolysin producing strain was isolated from Kum rivet estuary located in west part of Korea. In the process of identification the isolated strain was similar to V. mimicus but did not show characteristics of known Vibrio species; therefore, the strain was designated as Vibrio sp. D9 ( V. kumkang) tentatively and further identification study was carried out by comparing its bacteriological characteristics, Morphologically Vibrio sp. D9 was a typical straight roe with a polar flagellium. Among known Vibrio species no identical strains were found when using automatic bacteria identification system ($MicioLog^(TM)$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp. D9 showed 18 and 13 different responses as compared to V. mimicus and V. cholerae, respectively. Clear hemolysis zones were observed with the strain against human and sheep blood agar plate, Hemolytic toxicity was confirmed by strong vascular permeability and fatal toxicity against mouse was also observed. Thus the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. D9 were salinity of $0{\~}5.0{\%}$, pH of $6.4{\~}9.8$, temperature of $15{\~}41^{\circ}C$, respectively.

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Isolation and Identification of Methylotrophic Actinomycetes capable of Producing Anti-oral Cancer Activity (구강암에 대해 항암효과를 나타내는 methanol 자화 방선균의 분리 및 동정)

  • Kim, Jung;Kim, Sun-Sook
    • Journal of Korean society of Dental Hygiene
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    • v.1 no.2
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    • pp.193-200
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    • 2001
  • An appropriate amount of samples, collected from three each paddy forest, field and riverside soil near Taegu city, was suspended in sterile water and then diluted in order to isolation of antagonistic to oral cancer. The diluted samples were inoculated on separating medium in the routing spreading method. So, seven hundred and eighteen strains were isolated on HV agar and 220 strains were on methanol medium from soil samples. So, during the screening of anti-oral cancer activity from soil, we isolated microorganisms showing powerful antagonistic activity. Among them, No. 78 strain exhibited the most strongly anti-oral cancer activity. Microbiological properties were investigated by the methods described in the Bergey's Manual of Systematic Bacteriology and experimental methods of identification of actinomycetes by Hamada et al. As a result, a methylotrophic actinomycetes strain No. 79 was estimated as Amycolatopsis sp. based on taxonomic studies.

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Numerical Identification of a Streptomyces Strain Producing Spores in Submerged Culture

  • Rho, Yong-Taik;Kim, Hyoung-Tae;Oh, Kyoung-Hee;Kang, Heui-Il;Alan C. Ward;Michael Goodfellow;Hah, Yung-Chil;Lee, Kye-Joon
    • Korean Journal of Microbiology
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    • v.30 no.4
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    • pp.278-285
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    • 1992
  • Chemotaxonomic and numerical identification were carried out for a isolate of Streptomyces strain SMF301 producing spores in submerged culture. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate SMF301 was identified to cluster 1A of Streptomyces and best matched to Streptomyces limosus which is a synonym of Streptomyces albidoflavus. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces alidoflavus.

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Numerical Identification of an Actiomycetes Stain Producing an Antibiotic that Generates Oxygen Radical (활성산소를 발생하는 항생물질을 생성하는 방선균 분리균주의 수리동정)

  • Lee, Dong-Sun;Ha, Sang-Chul;Shin, Woo-Chang;Kim, Tae-Ho;Kim, Hong-Joong;Park, Yong-Ha;Kim, Jong-Guk;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
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    • v.23 no.4
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    • pp.397-404
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    • 1995
  • Chemotaxonomy and numerical identification were carried out for an isolate strain No.8001 producing an antibiotic that generates oxygen radical. The genus of strain No.8001 was decided as Streptomyces sp. from chemotaxonomic data. Thirty-nine taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces citreofluorescens in the major cluster 1B of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces citreofluorescens.

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Numerical study for identifying damage in open-hole composites with embedded FBG sensors and its application to experiment results

  • Yashiro, S.;Murai, K.;Okabe, T.;Takeda, N.
    • Advanced Composite Materials
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    • v.16 no.2
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    • pp.115-134
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    • 2007
  • This study proposes two new approaches for identifying damage patterns in a holed CFRP cross-ply laminate using an embedded fiber Bragg grating (FBG) sensor. It was experimentally confirmed that the reflection spectrum from the embedded FBG sensor was significantly deformed as the damage near the hole (i.e. splits, transverse cracks and delamination) extended. The damage patterns were predicted using forward analysis (a damage analysis and an optical analysis) with strain estimation and the proposed damage-identification method as well as the forward analysis only. Forward analysis with strain estimation provided the most accurate damage-pattern estimation and the highest computational efficiency. Furthermore, the proposed damage identification significantly reduced computation time with the equivalent accuracy compared to the conventional identification procedure, by using damage analysis as the initial estimation.

Identification of Adenosine Deaminase Inhibitor-producing Bacterium Isolated from Soil

  • SHIN, YONG KOOK;YONG-HA PARK;JAE-DONG LEE;HONG-KI JUN
    • Journal of Microbiology and Biotechnology
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    • v.7 no.1
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    • pp.32-36
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    • 1997
  • An adenosine deaminase inhibitor-producing bacterium was isolated from soil. An isolate exhibiting high adenosine deaminase inhibitory activity, was designated J-89, and classified as a strain of Bacillus subtilis on the basis of its morphological, phenotypic characteristics, the menaquinone content and cellular fatty acid composition. To confirm the taxonomic position of the strain we need more information such as DNA-DNA homology and other chemotaxonomic characteristics. In this paper we provisionally named strain J-89 as Bacillus sp. J-89 pending further chemotaxonomic study and analysis of adenosine deaminase inhibitor.

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Isolation and Identification of Antifungal Bacteria on Blue Mold in Apple (사과 푸른곰팡이병의 길항미생물의 분리 및 동정)

  • 이인선;조정일
    • Journal of Food Hygiene and Safety
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    • v.14 no.2
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    • pp.167-171
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    • 1999
  • In order to screen the antagonistic bacteria which inhibit the growth of the apple pathogen, Penicillum expansum, we isolated an effective bacterial strain and investigated into the antifungal activity of the antagonist and it's identification. The eleven strains of bacteria which strongly inhibited P. expansum were isolated from the nature, and the best antagonistic bacterial strain designated as CH142, was selected. The antagonistic strain CH142 was identified to be the genus Bacillus subtilis based on morphological and biochemical characterization. The CH142 showed 55.9% of antifungal activity against the growth of P. expansum. By the treatment of the culture broth and the heat treated culture filtrate of it, the B. subtilis CH142 showed 90% and 15% of antifungal activity, respectively.

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Genetic Identification of the Kimchi Strain Using PCR-based PepN and 16S rRNA Gene Sequence (PepN과 16S rRNA Gene Sequence 및 PCR 방법을 이용한 김치 젖산균의 동정)

  • Lee, Myung-Ki;Park, Wan-Soo;Lee, Byong-H.
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1331-1335
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    • 2000
  • The WL6 strain isolated from Kimchi could not be made scientific name because it was identified as three species, i.e., Leuconostoc mesenternides ssp cremoris, Leu. mesenteroides ssp. dextranicum or Lactobacillus bifermentans when it was tested by API kit or Biolog system methods. The unidentifiable WL6 strain was finally reclassified as Lactobacillus bifermentans by genetic identification using two PCR-based specific sequence primer sets which were originated from homologous pepN and 16S rRNA genes.

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Identification of isotropic and orthotropic constitutive parameters by FEA-free energy-based inverse characterization method

  • Shang, Shen;Yun, Gun Jin;Kunchum, Shilpa;Carletta, Joan
    • Structural Engineering and Mechanics
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    • v.45 no.4
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    • pp.471-494
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    • 2013
  • In this paper, identification of isotropic and orthotropic linear elastic material constitutive parameters has been demonstrated by a FEA-free energy-based inverse analysis method. An important feature of the proposed method is that it requires no finite element (FE) simulation of the tested material. Full-field displacements calculated using digital image correlation (DIC) are used to compute DIC stress fields enforcing the equilibrium condition and DIC strain fields using interpolation functions. Boundary tractions and displacements are implicitly recast into an objective function that measures the energy residual of external work and internal elastic strain energy. The energy conservation principle states that the residual should be zero, and so minimizing this objective function inversely identifies the constitutive parameters. Synthetic data from simulated testing of isotropic materials and orthotropic composite materials under 2D plane stress conditions are used for verification of the proposed method. When identifying the constitutive parameters, it is beneficial to apply loadings in multiple directions, and in ways that create non-uniform stress distributions. The sensitivity of the parameter identification method to noise in both the measured full-field DIC displacements and loadings has been investigated.