• Title/Summary/Keyword: Strain difference

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Sigma S Involved in Bacterial Survival of Ralstonia pseudosolanacearum (Ralstonia pseudosolanacearum 생존에 관여하는 Sigma S 역할)

  • Hye Kyung Choi;Eun Jeong Jo;Jee Eun Heo;Hyun Gi Kong;Seon-Woo Lee
    • Research in Plant Disease
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    • v.30 no.2
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    • pp.148-156
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    • 2024
  • Ralstonia pseudosolanacearum, a plant pathogenic bacterium that can survive for a long time in soil and water, causes lethal wilt in the Solanaceae family. Sigma S is a part of the RNA polymerase complex, which regulates gene expression during bacterial stress response or stationary phase. In this study, we investigated the role of sigma S in R. pseudosolanacearum under stress conditions using a rpoS-defective mutant strain of R. pseudosolanacearum and its wild-type strain. The phenotypes of rpoS-defective mutant were complemented by introducing the original rpoS gene. There were no differences observed in bacterial growth rate and exopolysaccharide production between the wild-type strain and the rpoS mutant. However, the wild-type strain responded more sensitively to nutrient deficiency compared to the mutant strain. Under the nutrient deficiency, the rpoS mutant maintained a high bacterial viability for a longer period, while the viability of the wild-type strain declined rapidly. Furthermore, a significant difference in pH was observed between the culture supernatant of the wild-type strain and the mutant strain. The pH of the culture supernatant for the wild-type strain decreased rapidly during bacterial growth, leading to medium acidification. The rapid decline in the wild-type strain's viability may be associated with medium acidification and bacterial sensitivity to acidity during transition to the stationary phase. Interestingly, the rpoS mutant strain cannot utilize acetic acid, D-alanine, D-trehalose, and L-histidine. These results suggest that sigma S of R. pseudosolanacearum regulates the production or utilization of organic acids and controls cell death during stationary phase under nutrient deficiency.

Insecticidal Activities and Synergism of Bistrifluron Against EPN-Resistant Strain of Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae) (EPN 저항성 배추좀나방에 대한 IGR계 약제 Bistrifluron의 살충활성과 세 가지 협력제에 의한 살충활성 증진 효과)

  • Joo, You-Lee;Yang, Jeong-Oh;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.13 no.1
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    • pp.54-61
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    • 2009
  • EPN-resistant diamondback moth were tested the developmental characteristics, longevity, fecundity and synergistic effect with synergists when treated with the bistrifluron, an inhibitor of chitin synthesis. Inhibition rate of egg hatch was below at the recommended concentration (50 ppm) to the EPN-resistant strain than EPN-non selected strain, however, mortality within 24hr after hatching was showed as 50-60%. They did not show the difference between two strains. Mortality of larval instars were showed effective to two strains with no difference. Inhibition rate of emergence did not show any effect when treated with pupae. Moreover, they did not affect to the longevity and fecundity. When bistrifluron was treated at 50 ppm to the adult, however, longevity and fecundity was decreased with no difference between two strains. Bistrifluron to the EPN-resistant strain showed synergistic effect as high as 137.7 and 73.1 folds with synergist such as PBO (microsomal oxidase inhibitor) and polyoxin B (chitin synthetase inhibitor), respectively.

Grain Evolution during Bulge Blow forming of AZ31 Alloy (AZ31 합금의 온간 부풀림 성형시 결정립 변화에 관한 연구)

  • Baek, S.G.;Lee, Y.S.;Lee, J.H.;Kown, Y.N.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2008.10a
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    • pp.452-455
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    • 2008
  • In the present study, blow forming characteristics of commercially roiled AZ31 alloy sheets were investigated. Two different kinds of AZ31 sheets were originally fabricated by using direct casting and strip casting methods respectively. Both sheets have similar grain sizes of about $7{\mu}m$ with a relatively equiaxed structure after rolling. A series of tensile tests were carried out to get flow behavior in terms of temperature and strain rate. Also, grain size effect was investigated by annealing as-received sheet at elevated temperatures. Elongation increased with temperature increment as well expected. However, the differences in tensile test condition did not give much difference in elongation even at the temperature range where a large elongation would be expected with such as fine grain of $7{\mu}m$. Blow forming experiments showed that forming condition did not result in higher difference in dome height. However, the interesting feature from this study was that formability of this AZ31 alloy got different with stress condition. Firstly, biaxial stress condition might result in lower temperature and strain rate dependencies compared to uniaxial tension results for both DC and SC sheets. Secondly, DC showed slower grain growth in uniaxial tension than in biaxial stress state while SC has much higher grain growth rage in uniaxial tension than in bulging.

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A Study on the Composite Insulation Boom Development of the Aerial Work Platform for Electric Work (전선작업용 고소작업차의 복합재 절연붐 개발)

  • Kang, Dongmyeong;Cho, Kyuchun;Hwang, Taekju;Sim, Kijoong;Guk, Ho-Hyeong;Kim, in-Soo;Park, Moon-ho
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.17 no.2
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    • pp.137-144
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    • 2018
  • This study describes the 3D design and analysis of a composite insulation boom of an aerial work platform for electric work. Structural analysis of the composite insulation boom was performed using ANSYS and the total deformation, equivalent stress, and normal elasticity were investigated at 150 mm, 500 mm, 1,000 mm, and 1,500 mm using a mounted strain gauge. The results of the analysis and test were similar in a linearly increasing slope and the difference in the strain value was about 10%. This difference depends on the compressive force in the manufacturing process.

Nodakenin and Decursin Contents of Fermented Angelicae Gigantis Radix by 4 Species Strain (4종의 발효균주가 당귀의 nodakenin과 decursin의 함량에 미치는 변화)

  • Park, Jin-Han;Jung, Ji-Wook;Kweon, Kee-Tae;Seo, Min-Jun;Seo, Eun-Kyoung;Park, Yong-Ki;Lee, Je-Hyun
    • The Korea Journal of Herbology
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    • v.25 no.4
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    • pp.7-10
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    • 2010
  • Objectives : The purpose of this study was to investigate the nodakenin and decursin contents in each fermented Angelicae gigantis Radix by 4 species of ferment strains. Methods : The strains of fermented Angelicae gigantis Radix were Lactobacillus plantarum(SE1), L. acidophilus(AC), Bacillus subtilis(B2) and B. licheniformis(BL2). The fermentative changes of nodakenin and decursin were analyzed using HPLC. Results : All of 4 species strains reduced nodakenin and decursin concentration in Angelicae gigantis Radix by fermentation. But fermentability were difference between strains. The nodakenin concentration of AC was lower than B2. The decursin concentrations of SE1 and AC were lower than B2 and BL2. Conclusions : 4 species of fermental strain were difference decomposable rate with nodakenin and decursin in fermented Angelicae gigantis Radix.

Effects of thickness variations on the thermal elastoplastic behavior of annular discs

  • Wang, Yun-Che;Alexandrov, Sergei;Jeng, Yeau-Ren
    • Structural Engineering and Mechanics
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    • v.47 no.6
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    • pp.839-856
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    • 2013
  • Metallic annular discs with their outer boundary fully constrained are studied with newly derived semi-analytical solutions for the effects of thickness variations under thermal loading and unloading. The plane stress and axisymmetric assumptions were adopted, and the thickness of the disk depends on the radius hyperbolically with an exponent n. Furthermore, it is assumed that the stress state is two dimensional and temperature is uniform in the domain. The solutions include the elastic, elastic-plastic and plastic-collapse behavior, depending on the values of temperature. The von Mises type yield criterion is adopted in this work. The material properties, Young's modulus, yield stress and thermal expansion coefficient, are assumed temperature dependent, while the Poisson's ratio is assumed to be temperature independent. It is found that for any n values, if the normalized hole radius a greater than 0.6, the normalized temperature difference between the elastically reversible temperature and plastic collapse temperature is a monotonically decreasing function of inner radius. For small holes, the n values have strong effects on the normalized temperature difference. Furthermore, it is shown that thickness variations may have stronger effects on the strain distributions when temperature-dependent material properties are considered.

The Effects of Light Intensity, Inoculum Size, and Cell Immobilisation on the Treatment of Sago Effluent with Rhodopseudomonas palustris Strain B1

  • Ibrahim, Shaliza;Vikineswary, S.;Al-Azad, Sujjat;Chong, L.L.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.5
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    • pp.377-381
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    • 2006
  • A study was carried out to determine a suitable light intensity and inoculum size for the growth of Rhodopseudomonas palustris strain B1. The pollution reduction of sago effluent using free and immobilised R. palustris cells was also evaluated. The growth rate in glutamatemalate medium was highest at 4 klux compared to 2.5 and 3 klux. The optimal inoculum size was 10% (v/v). Both the COD and BOD of the sago effluent were reduced by 67% after three days of treatment. The difference in biomass production or BOD and COD removal with higher inoculum sizes of 15 and 20% was minimal. This could be attributed to limited nutrient availability in the substrate. The use of immobilised cells of R. palustris reduced the pollution load 10% less compared to pollution reduction by free cells. Hence, there was no significant difference in using free or immobilised cells for the treatment of sago effluent.

Regional Distribution of Barley Yellow Dwarf Virus Strains in Korea and Identification of Resistant Wheat

  • Woo, Mi-Ok;Park, Hyung-Ho;Nam, Jung-Hyun;Paek, Nam-Chon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.1
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    • pp.57-63
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    • 2001
  • Barley Yellow Dwarf Virus (BYDV) has been a major disease causing a severe loss of yield in winter cereals worldwide. It has been recently reported that BYDV occurs frequently in wheat field and also causes serious yield reduction in Korea. This study was performed to investigate the regional distributions of BYDV strains in Korea and to identify the resistant cultivars or lines of wheat to the predominant BYDV strains, providing basic information for the breeding of BYDV-resistant wheat varieties. Using RT-PCR and EcoRI digestion methods, the regional distribution of BYDV strains in Korea from 1999 to 2000 showed that PAV strain was mainly detected about 65% (Vic-PAV 52.6% ; CN-PAV 47.4%) and MAV strain about 3%. Using ELISA test for the examination of BYDV resistance with 17 cultivars and 4 lines among Korean wheat, three cultivars, Gurumil, Topdongmil, and Olgurumil, were susceptible to BYDV and the others were resistant. In plant growth and yield component responses to BYDV infection, Gurumil showed significant difference between the uninfected and the infected, suggesting the most susceptible to BYDV among Korean wheat, but Eun-pamil and Seohae118 did no difference, an indication that they have the highest resistance.

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Chronic Health Conditions, Depression, and the Role of Financial Wellbeing: How Middle Age Group (45-64) and Older Adults (65-79) Differ?

  • Cha, Seung-Eun;Kim, Jin-Hee;Anderson, Elaine
    • International Journal of Human Ecology
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    • v.12 no.2
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    • pp.77-93
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    • 2011
  • This study investigates the association between chronic health conditions (CHD) and depression with a focus on the mediating effect of financial strain. We tested if age makes any difference in the effect of CHD and financial strain on depression. The data comes from the 2006 Korea Longitudinal Study of Aging (KLoSA) collected by the Institute of Korean Labor Research. The sample consisted of information from 8,961 individuals ages 45-79. Separate analyses were done for middle-age (45-64) and older-adult groups (65-79). There were significant financial portfolio differences among CHD patients and non-CHD, for both age groups, that may constitute the impact of a health event on financial wellbeing; in addition, the associations of CHD on depressive symptoms were different by age groups. The mediating effect of financial wellbeing on the association between CHD and depressive symptoms was verified; in addition, the role of financial wellbeing on the association was especially strong for the older-adult group. The effect of CHD on depression was contingent on the amount of net assets and annual personal income. Implications are discussed based on the findings.

The Effect of Flame Radiation on NOx Emission Characteristics in Hydrogen Thrbulent Diffusion Flames (수소 난류확산화염에서 NOx 생성특성에 대한 화염열복사의 영향)

  • Kim, Seung-Han;Kim, Mun-Ki;Yoon, Young-Bin
    • Journal of the Korean Society of Combustion
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    • v.5 no.2
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    • pp.51-62
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    • 2000
  • The relationship among the flame radiation, NOx emissions, residence time, and global strain rate are examined for turbulent non-premixed jet flames with wide variations in coaxial air conditions. Measurements of NOx emission, flame geometry and flame radiation were made to explain the NOx emission scaling based on global parameters such as flame residence time, global strain rate, and radiant fraction. The overall 1/2-power scaling is observed in coaxial air flames, irrespective of coaxial air conditions, but the degree of deviation from the 1/2-slope curve in each case differs from one another. From the comparison between the results of pure hydrogen flames and those of helium diluted hydrogen flames, it is observed that flame radiation plays a significant role in pure hydrogen flames with coaxial air and the deviation from 1/2-power scaling may be explained in two reasons: the difference in the flame radiation and the difference in jet similarity in coaxial air flames. From the radiation measurements, more detailed explanations on these deviations were suggested.

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