• KSBB Journal
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• 제4권1호
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• pp.11-14
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• 1989

Tower fermentor를 이용한 연속 알콜발효에서 cell re-cycle과 aeration에 대한 영향을 검토하였다. 균주는 floc-culationg 효모인 Saccharomyces cerecisiae TS4를 를 사용하였다. 15% glucose를 사용한 cell recycle system의 연속 알콜발효에서 cell 농도는 50%/1였고, ethanol productiv는 26.4g EtOH/l-hr로서 cell농도가 가장 높은 값을 나타내었으며, aeration rate는 3.8$\times$ $10^-^3$ VVM이상부터는 ethanol pro-ductivity가 감소하였다.

• 한국농공학회논문집
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• 제47권5호
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• pp.27-39
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• 2005

In the recent design of high ductile fiber-reinforced ECC (engineered cementitious composite), optimizing both processing and mechanical properties for specific applications is critical. This study presents an innovative method to develop new class ECCs, which possess the different fluid properties to facilitate diverse types of processing (i.e., self-consolidating or shotcrete processing) while maintaining ductile hardened properties. In the material design concept, we employ a parallel control of fresh and hardened properties by using micromechanics and cement rheology. Control of colloidal interaction between the particles is regarded as a key factor to allow the performance of the specific processing. To determine how to control the particle interactions and the viscosity of cement suspension, we first introduce two chemical admixtures including a highly charged polyelectrolyte and a non-ionic polymer. Optimized mixing steps and dosages we, then, obtained within the solid concentration predetermined based on micromechanical principle. Test results indicate that the rheological properties altered by this approach were revealed to be highly effective in obtaining the desired function of the fresh ECC, allowing us to readily achieve hardened properties, represented by pseudo strain-hardening behavior in uniaxial tension.

• 대한기계학회논문집A
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• 제21권5호
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• pp.842-849
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• 1997

The aim of this study is an investigation of the interaction of two micro hole defects affecting fatigue crack initation life and propagation behavior. The locatio of two micro hole defects was considered as an angle of alignment and the distance between the centers of two micro hole defects. The fatigue cracking behavior is experimented under bending. When micro defects are located close to each other, the fatigue crack initiation lives are varied with their relative locations. In the experiments, the area of local plastic strain strongly played a role in the fatigue crack initiation lives. Therefore we introduce a parameter which contains the plastic deformation area at stress concentrations and propose a fatigue crack initiation life prediction curve. In addition, the directions and propagation rates of fatigue cracks initiated at two micro hole defects are studied experimentally.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권1호
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• pp.1-6
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• 2000

For the rapid selection of higher recombinant hirudin producing strain in a methylotrophic yeast Hansenula polymorpha, a multiple gene integration and dose-dependent selection vector, based on a telomere-associated ARS and a bacterial aminoglycoside 3-phosphotransferase (aph) gene, was adopted. Two hirudin expression cassettes (HV1 and HV2) were constructed using the MOX promoter of H. polymorpha and the mating factor $\alpha$ secretion signal of S. cerevisiae. Multiple integrants of a transforming vector containing hirudin expression cassettes were easily selected by using an antibiotic, G418. Hirudin expression level and integrated plasmid copy number of the tested transformants increased with increasing the concentration of G418 used for selection. The expression level of HV1 was consistently higher than that of HV2 under the similar conditions, suggesting that the gene context might be quite important for the high-level gene expression in H. polymorpha. The highest hirudin producing strain selected in this study produced over 96 mg/L of biologically active hirudin in a 500-mL flask and 165 mg/L in a 5-L fermentor.

• 한국화재소방학회논문지
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• 제17권4호
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• pp.111-116
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• 2003

가로, 세로, 높이가 각각 100cm, 60cm, 45cm로 내용적이 $270\ell$인 폭발 용기를 이용하여 불균일 농도 상태의 LPG-공기 혼합가스의 폭발특성을 측정하였다. 폭발은 vented-explosion과 closed explosion의 조건에서 실시하였다. 실험의 변수로는 점화원의 위치, 노즐직경 및 유속으로, 시료가스를 주입하는 노즐의 직경과 유속을 변화시키면서 용기 내에서의 불균일 혼합정도를 조절하였다. 폭발압력은 strain형 압력센사를 사용하여 측정하였고 폭발화염의 거동은 비디오카메라로 측정하여 분석하였다. 실험결과 유속과 가스 주입 시간이 용기 내 가스 혼합에 중요한 요소임을 알 수 있었으며, 불균일 정도가 심화될 수록 폭발압력과 압력상승속도가 감소하였으나 용기 내 폭발화염의 체류시간은 크게 증가하였으며 이로 인하여 가스 폭발 후 화재로의 전이 위험성이 증가함을 알 수 있었다.

• 한국식품저장유통학회지
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• 제30권1호
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• pp.65-77
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• 2023

In this study, we investigated the immunological properties of six strains of Acetobacter pasteurianus through nuclear factor-kappa B/activator protein-1 (NF-κB/AP-1) transcription factor activation and nitric oxide (NO) and cytokine production in macrophages. We found that the six A. pasteurianus strains had no significant inhibitory effect on the cell viability of RAW-Blue^TM cells at the concentration of (25, 50, 100 CFU/macrophage). The production of NO and cytokines (TNF-α, IL-1β, and IL-6) showed different abilities of immune activation for each strain, and it was 0.7 to 0.9 times higher than that of the LPS (100 ng/mL, v/v) positive control and 7 to 8 times superior to that of the negative control group. To explore the underlying mechanism, we evaluated the mRNA expression of pro-inflammatory genes. Consequently, we found that inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression including genes expression of cytokines were elevated by the six A. pasteurianus treatment. These results suggested that the six strains of A. pasteurianus have an excellent industrial application value as a functional material for the purpose of enhancing immune function.

• 한국정밀공학회지
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• 제16권4호통권97호
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• pp.122-128
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• 1999

Bolted joint assembly for nuclear power plants consists of various components : cover plate, retainer plate, manway flange, gasket and stud bolts/nuts. To guarantee the soundness of the joint, it is important to prevent leakage through the gasket and reduce the stress concentration factor at the thread root. In this paper, Submodeling technique for the finite element method is proposed to accurately compute three dimensional contact stresses which govern the sealing performance and the maximum contact stresses at the threads root. For verification of global solutions used as boundary conditions of submodel solution, the stresses on the cover plate and the manway flange are measured by strain gages when internal pressure is applied to the bolted joint assembly. The numerical results are compared with the experimental results.

• International Journal of Naval Architecture and Ocean Engineering
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• 제4권2호
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• pp.132-140
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• 2012

This study aims to assess fatigue property by the static overload and average load in the fillet welded joints which is on the ship structural steel having gusset welds. To this end, a small specimen was made, to which the same welding condition for the actual ship structure was applied, to perform fatigue tests. In this study, a method to simply assess changes in welding residual stress according to different static overload was suggested. By measuring actual strain at the weld toe, the weld stress concentration factor and property which is determined by recrystallization in the process of welding were estimated to investigate the relation between overload and fatigue strength.

• Structural Engineering and Mechanics
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• 제69권1호
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• pp.69-75
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• 2019

The plates made of two materials joined to each other having the different coefficient of thermal expansions are frequently encountered in the industrial applications. The stress analysis of these members under the effect of high-temperature variation has great importance in design. In this study, the stress analysis of the experimental model developed for the problem considered here was performed by the method of photothermoelasticity. The thermal strains were formed by the mechanical way and these were fixed by the strain freezing method. For the stress measurements, the method of slicing is applied which provides three-dimensional stress analysis. The analytical solution in the literature was compared with the related stress distribution obtained from the model. Moreover, the axisymmetric finite element model developed for the problem was solved by ABAQUS and the results obtained here compared with those of the experimental model and the analytical solution. As a result of this study, this experimental method and numerical model can be used for these type of thermal stress problems which have not been comprehensively analyzed yet.

• 한국전기전자재료학회논문지
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• 제35권5호
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• pp.516-521
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• 2022

We investigated the microstructure, crystal structure, dielectric, and elecromechanical strain properties of lead-free BaTiO₃ (BT)-modified (Bi_1/2Na_1/2)TiO₃-SrTiO₃ (BNT-ST) piezoelectric ceramics. Samples were prepared by a conventional ceramic processing route. Temperature dependent dielectric properties confirmed that a phase transition from a nonergodic relaxor to an ergodic relaxor was induced when the BT concentration reached 1.5 mol%, interestingly, where the average grain size reached a maximum value of 4.5 ㎛. At the same time, enhanced electromechanical strain (S_max/E_max = 600 pm/V) was obtained. It is suggested that the induced ferroelectric-relaxor phase transition by the BT modification is responsible for the enhancement of electromechanical strain in 1.5 mol% BT-modified BNT-ST ceramics.