• Title/Summary/Keyword: Strain competition

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Role of Dual Flagella in the Pathogenesis of Vibrio parahaemolyticus

  • Lee, Hwa-Gyu;Jeong, Byung-Gon;Park, Kwon-Sam
    • Fisheries and Aquatic Sciences
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    • v.14 no.2
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    • pp.73-78
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    • 2011
  • Vibrio parahaemolyticus possesses two flagella systems: polar and lateral flagella for swimming in liquid and swarming on solid surfaces or in viscous environments. To elucidate the pathogenic role of these dual flagella systems, we constructed single- and double-deletion mutants of the lafA and flhAB flagellum genes and investigated their biofilm formation, cell adhesion, and colonization of the small intestine of suckling mice. The double-mutant strain was more impaired in biofilm formation than either of the single-mutant strains. In addition, the lafA, flhAB, and double-mutant strains showed 40%, 45%, and 60%, respectively, lower adherence to HeLa cells than the wild-type strain. Moreover, the lafA, flhAB, and double-mutant strains exhibited 49%, 5.6 and 6.7 times, respectively, lower colonization in a competition assay than the wild-type strain. These findings indicated that polar flagella were more important than lateral flagella for the pathogenesis of V. parahaemolyticus.

Competition and Host-strain Interaction of Soybean Rhizobium Strains on Two Soybean Cultivars (콩 근류균계간 경합과 숙주 친화성의 품종간 차이)

  • 박의호;싱글톤폴
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.6
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    • pp.718-724
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    • 1996
  • Two soybean cultivars, ‘Lee’ and ‘Peking’, were used to evaluate the competition and interaction of rhizobium strains PRC205 (R. fredii, fast-grower) and USDA110 (B. japonicum, slow-grower). Strains were inoculated separately on the root parts of a split-root growth system. Both root sides were inoculated simultaneously with four combinations of strain treatment to evaluate the competition of strains. And to evaluate the interaction of strains one side of split-root system was inoculated a week prior to the other side. Nodule mass and dry weight of the plants were measured 3 weeks after treatments. PRC205 showed no effective nodulation and no competing ability with USDA110 on Lee cultivar, however, contrary results on Peking cultivar. Top dry weight of Lee inoculated with PRC205 was much lower than that of any other inoculation treatments, however, in Peking that with PRC205 was higher than that with USDA110. There were no differences in root dry weight among the inoculation treatments. USDA110 used as primary inoculant suppressed nodule mass of opposite side, secondary inoculant, severely in both cultivars. PRC205 showed same tendency as USDA110 in Peking, but revealed little suppression effects on USDA110 used as secondary inoculant in Lee. USDA110 used as primary inoculant in Lee and PRC205 in Peking showed much more dry weight of soybean plants than that of other treatments.

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The Effects of Molybdenum Content on the Dynamic Properties of Tungsten-based Heavy Alloys

  • Lee, Woei-Shyan;Chan, Tien-Yin
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09b
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    • pp.1155-1156
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    • 2006
  • Hopkinson bar dynamic test under strain rates ranging from 2000 $s^{-1}$ to 8000 $s^{-1}$ at room temperature revealed that the flow stress of tungsten heavy alloys depended strongly on the strain, strain rate, and the content of molybdenum. The variation of flow stress was caused by the competition between work hardening and heat softening in the materials at different strain rates. The high temperature strength of the matrix phase was increased by the addition of molybdenum, which enhanced the strength of the tungsten heavy alloys in high strain rate test.

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Suppression of Bacterial Wilt with Fuorescent Pseudomonads, TS3-7 strain (Fluorescent siderophore 생산균주, TS3-7에 의한 풋마름병 발병 억제)

  • Kim, Ji-Tae;Cho, Hong-Bum;Kim, Shin-Duk
    • Applied Biological Chemistry
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    • v.48 no.3
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    • pp.296-300
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    • 2005
  • Among the root colonizing and plant growth promoting bacteria isolated from the bacterial wilt suppressive soil, five strains were detected to produce siderophores by CAS agar assay. The most effective isolate, TS3-7 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 80% reduction of bacterial wilt disease compared with the control. Significant disease suppression by TS3-7 strain was related to the production of siderophore. Besides iron competition, induction of resistance of the host plant with siderophore was suggested to be another mode of action that suppress bacterial wilt, based on the lack of direct antibiosis against pathogen in vitro. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, TS3-7 stain was identified as Pseudomonas sp. TS3-7.

Studies on Combustion Characteristics and Reduced Kinetic Mechanisms of Natural Gas Premixed Flames (천연가스 예혼합화염의 연소특성 및 축소반응메커니즘에 관한 연구)

  • 이수룡;김홍집;정석호
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.4
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    • pp.166-177
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    • 1998
  • Combustion characteristics of natural gas premixed flames is studied experimently and numerically by adopting a counterflow as a flamelet model in turbulent flames. Flame speeds are measured by employing LDV, and the results show that flame speed increases linearly with strain rate, which agrees well with numerical results. Parametric dependences of extinction strain rates are studied numerically with detailed kinetic mechanism to show that the addition of ethand to a methane premixed flame makes the flame more resistant to strain rate. The effect of pressure on the extinction strain rate is that the extinction strain rate increases up to 10 atm and them decreases, which is explained by competition of chain branching H+O2=OH+O and recombination reaction H+O2+M=HO2+M. Detailed mechanism having seventy-four step is systematically reduced to a nine-step and a five-step thermal NOx chemistry is reduced to two-step. Comparison between the results of the detailed and the reduced mechanisms demonstrates that the reduced mechanism successfully describes the essential features of natural gas premixed flames including extinction strain rate and NOx production.

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Effect of competition between superconductivity and ferromagnetism in GdBa2Cu3O7-x/La0.7Sr0.3MnO3 bilayers

  • Oh, Jun-Yung;Yang, Dong-Seok;Kang, Byeongwon
    • Progress in Superconductivity and Cryogenics
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    • v.24 no.2
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    • pp.19-22
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    • 2022
  • We studied the effect of substrate-induced strain state on the superconducting transition in GdBa2Cu3O7-x(GdBCO)/La0.7Sr0.3MnO3 (LSMO) bilayers deposited on a LaAlO3 (LAO) substrate. The stain state of LSMO is controlled by increasing the thickness from 20 nm to 80 nm. Analyses on the extended X-ray absorption fine structure (EXAFS) measurements reveal difference in the direction of MnO6 octahedral distortion depending on the LSMO thickness, which leads to a difference in anisotropy of magnetization of LSMO layer. The superconducting transitions of our system are strongly correlated with the magnetic anisotropy accompanied by the MnO6 octahedron distortion in a specific direction. This result suggests the possibility of improving the superconducting transition in the GdBCO/LSMO bilayer system by controlling the degree of competition between superconductivity and ferromagnetism via adjusting strain state in the LSMO layer.

Development of Textures and Microstructures during Compression in a Hot-Extruded AZ31 Mg Alloy (고온압출한 AZ 31 마그네슘 합금의 압축변형 중 집합조직과 미세조직의 발달)

  • Jung, Byung Jo;Lee, Myung Jae;Park, Yong-Bum
    • Korean Journal of Metals and Materials
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    • v.48 no.4
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    • pp.305-314
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    • 2010
  • The development of textures and microstructures during plastic deformation in a hot-extruded AZ 31 Mg alloy was investigated using a compression test with such parameters as deformation temperature, strain rate. It was observed from true stress-strain curves that twinning involves changes of the flow stresses. In the early stages of deformation at temperatures lower than $200^{\circ}C$, the occurrence of twins resulted in a decrease of the work-hardening rate, which increased drastically at a true strain of -0.05. The evolution of the deformation textures were assessed with the aid of EBSD analyses in terms of the competition between twinning and slip activity.

Control of Microalgal Growth and Competition by N: P Ratio Manipulation (N: P ratio 조절에 의한 미세조류 생장과 경쟁 제어)

  • Ahn, Chi-Yong;Lee, Jae-Yon;Oh, Hee-Mock
    • Korean Journal of Environmental Biology
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    • v.31 no.2
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    • pp.61-68
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    • 2013
  • Microalgae can grow autotrophically with the supply of light, carbon dioxide and inorganic nutrients in water through photosynthesis. Generally, microalgal growth is limited by the concentrations and relative ratio of nitrogen (N) and phosphorus (P) among the nutrients in the aquatic environment. Each microalga has its specific optimum N : P ratio resulting in dominance in a particular water having similar nutrient composition. Algal bloom is an immense growth of certain microalga commonly cyanobacterium and can be sequestrated by reducing the limiting nutrient, generally P in the freshwater. Moreover, dominance of a less toxic blooming strain can be established by manipulating N : P ratio in the water. On the other hand, microalgal biomass of a certain species can be enhanced by increasing limiting nutrient and adjusting the N : P ratio to the target species. The above-mentioned eco-physiological features of microalgae can be more completely interpreted in connection with their genomic informations. Consequently, microalgal growth regulation which can be achieved on the basis of its eco-physiological and further genomic insights would be helpful not only in the control of algal bloom, but also for an increased yield of algal biomass.

Evolution of Growth Orientation and Surface Roughness During Sputter Growth of AIN/Si(111) (스퍼터링 방법에 의한 AIN/Si(111)의 성장 방향과 표면 거칠기의 성장 시간에 대한 연구)

  • 이민수;이현휘;서선희;노동영
    • Journal of the Korean Vacuum Society
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    • v.7 no.3
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    • pp.237-241
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    • 1998
  • The growth orientation and the surface roughness of AIN/Si(111) films grown by radio frequency (RF) reactive magnetron sputtering were investigated using in-situ x-ray scattering technique and atomic force microscopy (AFM). AIN films were initially grown with the <001> preferred growth orientation under most growth conditions. As the film gets thicker, however, the growth orientation changes significantly, especially at high substrate temperature and high RF powers. We attribute the observed behavior to the competition between the surface energy that prefers the <001> growth orientation and the strain energy that randomizes the growth orientation. In addition, we investigated the evolution of the surface morphology during the growth using the x-ray reflectivity measurement.

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The Influence of Microstructure on the Bauschinger Effect in X80 Grade API Steel (X80급 API 강의 바우싱거 효과에 미치는 미세조직의 영향)

  • Park, J.S.;Kim, D.W.;Chang, Y.W.
    • Transactions of Materials Processing
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    • v.15 no.2 s.83
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    • pp.118-125
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    • 2006
  • API steel is used for line-pipe to transport the oil and natural gas. As the recent trends in the development of API steel are towards the use of larger diameter and thicker plate, many researches have been studied to achieve higher strength, higher toughness and lower yield ratio in API steel. However, the strength of API steel after pipe forming is changed depending on the competition of the Bauschinger effect and work hardening which are affected by the strain history during pipe forming process. So, the purpose of this study is to investigate the influence of microstructure on the Bauschinger effect for API steel. To change the microstructure of API steel we have changed the hot rolling condition and the amounts of V and Cu addition. The compression-tensile test and the microstructure observation by OM and TEM were conducted to investigate the yield strength drop and the correlation between the Bauschinger effect and microstructure of API steel. The experimental results show that the increase of polygonal ferrites volume fraction increases the Baushcinger effect due to the back stress which comes from the increase of mobile dislocation density at polygonal ferrite interior during the compressive deformation. The hot rolling condition was more effective on the Bauschinger effect in API steel than the small amount of V and Cu addition.