• Structural Engineering and Mechanics
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• 제5권3호
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• pp.239-256
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• 1997

The companion paper presents a new three-parameter model for the uniaxial rate-sensitive material response, which is based on a bilinear static stress-strain relationship with kinematic strain-hardening. This paper extends the proposed model to trilinear static stress-strain relationships for steel and concrete, and discusses the implementation of the new models within an incremental-iterative solution procedure. For steel, the three-parameter rate-function is employed with a trilinear static stress-strain relationship, which allows the utilisation of different levels of rate-sensitivity for the plastic plateau and strain-hardening ranges. For concrete, on the other hand, two trilinear stress-strain relationships are used for tension and compression, where rate-sensitivity is accounted for in the strain-softening range. Both models have been implemented within the nonlinear analysis program ADAPTIC, which is used herein to provide verification for the models, and to demonstrate their applicability to the rate-sensitive analysis of steel and reinforced concrete structures.

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.401-407
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• 2014

Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ${\Delta}luxS$ (ECM101) and ${\Delta}luxS{\Delta}relA{\Delta}spoT$ (ECM201), and the ${\Delta}luxS$ complement strain to ECM201 (ECM202) were created from EHEC O157:H7 EDL933 to investigate how the regulatory systems interact. The phenotypic changes of the mutant strains were characterized and compared with the wild type. The mutant strains exhibited no obvious growth defects, although acid resistance and cellular cytotoxicity were decreased significantly in all the mutant strains. Phenotypic characterization revealed that mutations in the stringent response system (ECM201 and ECM202) influenced the metabolic (defective utilization of arabinose and L-sorbose) and enzymatic activities (decreased trypsin activity, and increased ${\alpha}$-glucosidase activity). In contrast, the quorum sensing system mutant (ECM101) did not display these phenotypes. The motility of the quorum sensing system mutant (ECM101) was unchanged, but mutation in the stringent response system influenced the motility. Our results suggest that quorum sensing interacts with the stringent response regulation system.

• 한국도로학회논문집
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• 제14권2호
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• pp.11-17
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• 2012

본 연구는 실험계획법(예: 반응표면계획법) 및 하모니 검색 알고리즘을 통하여 다양한 아스팔트 콘크리트 포장 구조체에 있어 피로균열의 공용성 인자인 인장변형률을 예측하는 모델을 개발하는 방법에 대한 연구이다. 인장변형률을 산정하기 위하여 한국건설기술연구소에서 개발한 유한요소 축대칭해석 프로그램인 KICTPAVE를 이용하여 아스팔트 층과 린콘크리트 층의 접속면에서 발생되는 변형률을 구하여 데이터베이스(D/B)화 하였다. 아스팔트 포장에서 입력변수인 층별 탄성계수 및 두께를 다양한 조건에서 KICTPAVE 프로그램을 수행하여 훈련용 D/B(Training Set)인 변형률의 값들을 구축한 후 반응표면계획법에 근거하여 회귀방정식을 정의하였으며 방정식에 필요한 계수값을 결정하기 위하여 하모니 검색 알고리즘을 이용하였다. 최종적으로 결정된 회귀방정식의 계수값들의 정확성을 검증하기 위해서 훈련용 D/B가 아닌 다른 조건의 입력변수를 이용하여 검증용 D/B(Testing Set)를 구축하고 이를 이용하여 개발된 모델을 검증하였다.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.6-14
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• 2022

Brucella spp. are facultative intracellular pathogens that invade, survive and proliferate in numerous phagocytic and non-phagocytic cell types, thereby leading to human and animal brucellosis. Outer membrane proteins (Omps) are major immunogenic and protective antigens that are implicated in Brucella virulence. A strain deleted of the omp16 gene has not been obtained which suggests that the Omp16 protein is vital for Brucella survival. Nevertheless, we previously constructed an omp16 conditional deletion strain of Brucella, ∆Omp16. Here, the virulence and immune response elicted by this strain were assessed in a mouse model of infection. Splenomegaly was significantly reduced at two weeks post-infection in ∆Omp16-infected mice compared to infection with the parental strain. The bacterial load in the spleen also was significantly decreased at this post-infection time point in ∆Omp16-infected mice. Histopathological changes in the spleen were observed via hematoxylin-eosin staining and microscopic examination which showed that infection with the ∆Omp16 strain alleviated spleen histopathological alterations compared to mice infected with the parental strain. Moreover, the levels of humoral and cellular immunity were similar in both ∆Omp16-infected mice and parental strain-infected mice. The results overall show that the virulence of ∆Omp16 is attenuated markedly, but that the immune responses mediated by the deletion and parental strains in mice are indistinguishable. The data provide important insights that illuminate the pathogenic strategies adopted by Brucella.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 추계학술대회 논문집
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• pp.386-389
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• 2008

Forming limit diagram is created by graphical illustration indicating major and minor strain. In order to provide the criterion for forming safety, FLC(forming limit curve) need to be fitted to the diagram. However, the standard method for the strain measurement and FLC plotting are not fixed yet, which results in inconvenience in digitalized analysis. In this study, new construction method for FLC was suggested in order to minimize operator dependency. For this purpose, major and minor strain were measured automatically and analyzed. Then, a second order equation is adopted to fit the FLC. Optimized by response surface method was performed to ensure particular characteristics of the FLC.

• 대한기계학회논문집
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• 제17권8호
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• pp.1931-1939
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• 1993

The wave propagation characteristics of laminated composites subjected to a transverse high-velocity impact of a steel ball is investigated. For this purpose, high-velocity impact experiments were conducted to obtain the strain response histories, and a finite element analysis based on the higher-order shear deformation theory in conjunction with the static contact law is used. Test materials for investigation are glass/epoxy laminated composite materials with $[0^{\circ}/45^{\circ}/0^{\circ}/-45^{\circ}]_{2s}$ and $[90^{\circ}/-45^{\circ}/90^{\circ}-45^{\circ}/90^{\circ}]_{2s}$ stacking sequences. As a result, the strain responses obtained from the experiments represented the wave propagation characteristics in the transversely impact, also the wave propagation velocities obtained from high-velocity impact experiments and wave propagation theory agree well.

• Structural Engineering and Mechanics
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• 제38권1호
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• pp.81-97
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• 2011

In this research, the wavelet transform is used to analyze time response of a cracked beam carrying moving mass for damage detection. In this respect, a new damage detection method based on the combined use of continuous and discrete wavelet transforms is proposed. It is shown that this method is more capable in making damage signature evident than the traditional two approaches based on direct investigation of the wavelet coefficients of structural response. By the proposed method, it is concluded that strain data outperforms displacement data at the same point in revealing damage signature. In addition, influence of moving mass-induced terms such as gravitational, Coriolis, centrifuge forces, and pure inertia force along the deflection direction to damage detection is investigated on a sample case. From this analysis it is concluded that centrifuge force has the most influence on making both displacement and strain data damage-sensitive. The Coriolis effect is the second to improve the damage-sensitivity of data. However, its impact is considerably less than the former. The rest, on the other hand, are observed to be insufficient alone.

• Journal of Mechanical Science and Technology
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• 제17권7호
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• pp.1011-1015
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• 2003

The objectives of this work focus on the differences in responses of paired tendons from different anatomical locations. Tendon specimens were obtained from the hindlimbs of canines and frozen to -70$^{\circ}C$. After being thawed, specimens were mounted in the immersion bath, preloaded to 0.13N, and then subjected to 3% or 4% of the initial length at a strain rate of 5%/sec. It was found that the mechanical responses of anatomically paired tendons were nearly the same within each pair but different between pairs of tendons from different anatomical locations. Although flexor tendons had much larger cross-sectional area than the others, such as peroneus or extensor tendons, the stiffness of the flexor tendons were much lower than the others throughout their stress-strain responses. The nature and causes of these differences in the stiffness are not fully known. However, it is clear that differences in the mechanical response of tendons and other connective tissues are significant to the musculoskeletal performance.

• The Korean Journal of Ceramics
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• 제6권3호
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• pp.201-205
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• 2000

Fiber reinforced plastic (FRP) composites and ceramic matrix composites (CMC) which contain electrically conductive phases have been designed and fabricated to introduce the detection capability of damage/fracture detection into these materials. The composites were made electrically conductive by adding carbon and TiN particles into FRP and CMC, respectively. The resistance of the conductive FRP containing carbon particles showed almost linear response to strain and high sensitivity over a wide range of strains. After each load-unload cycle the FRP retained a residual resistance, which increased with applied maximum stress or strain. The FRP with carbon particles embedded in cement (mortar) specimens enabled micro-crack formation and propagation in the mortar to be detected in situ. The CMC materials exhibited not only sensitive response to the applied strain but also an increase in resistance with increasing number of load-unload cycles during cyclic load testing. These results show that it is possible to use these composites to detect and/or fracture in structural materials, which are required to monitor the healthiness or safety in industrial applications and public constructions.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2000년도 추계학술발표대회 논문집
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• pp.157-160
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• 2000

In author's previous paper, a finite element algorithm was presented to calculate the buckling and crippling stresses of composite laminated stringers. In this study, for the validation of the finite element analysis, Z-section composite stringers of different lengths and flange-widths were tested in axial compression. The stacking sequence of graphite/epoxy is [$\pm$45/0/90]s. Strain gages were attached to each specimen to get the strain response. Deflection and end-shortening were obtained by a displacement transducer. The buckling and crippling loads are determined from the strain response, load vs. end-shortening curves, and load vs. out-of-plane deflection curves. Comparison between finite element and experimental results shows good agreement in the buckling, local buckling, and crippling stresses.