• Biomedical Science Letters
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• v.15 no.3
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• pp.229-232
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• 2009

DNA isolation for PCR-based short tandem repeat (STR) analysis is essential to recover high yields of amplifiable DNA from low copy number (LCN) DNA samples. There are different methods developed for DNA extraction from the small bloodstain and gloves, commonly found at crime scenes. In order to obtain STR profiles from LCN DNA samples, DNA extraction protocols, namely the automated $iPrep^{TM}$ $ChargeSwitch^{(R)}$ method, the automated $QIAcube^{TM}$ method, the automated $Maxwell^{(R)}$ 16 DNA $IQ^{TM}$ Resin method, and the manual $QIAamp^{(R)}$ DNA Micro Kit method, were evaluated. Extracted DNA was quantified by the $Quantifiler^{TM}$ Human DNA Quantification Kit and DNA profiled by $AmpFISTR^{(R)}$ $Identifiler^{(R)}$ Kit. Results were compared based on the amount of DNA obtained and the completeness of the STR profiles produced. The automated $iPrep^{TM}$ $ChargeSwitch^{(R)}$ and $QIAcube^{TM}$ methoas produced reproducible DNA of sufficient quantity and quality trom the dried blood spot. This two automated methods showed a quantity and quality comparable to those of the forensic manual standard protocols normally used in our laboratory. In our hands, the automated DNA extraction method is another obvious choice when the forensic case sample available is bloodstain. The findings of this study indicate that the manual simple modified $QIAamp^{(R)}$ DNA Micro Kit method is best method to recover high yields of amplifiable DNA from the numerous potential sources of LCN DNA samples.

• KSBB Journal
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• v.7 no.1
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• pp.79-83
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• 1992

The characteristics of Erwinia rhapontici cells with $\alpha$-glucosyltransferase activity immobilized in Ca-alginate beads and the performance of two different types of reactor-stirred tank reactor(STR) and packed bed reactor(PBR)-charged with these immobilized cells to produce palatinose from sucrose were investigated. The optimal pH(5.5-6.0) and temperature($30-35^{\circ}C$) showed no appreciable difference between free and immobilized cells. The apparent Km value of the immobilized cells(0.28M) was approximately two times higher than that of free cells(0.13M) at $30^{\circ}C$. The half life of the immobilized cells was found to be 380 h with STR while much greater operational stability was achieved with PBR. Continuous operation of PBR at a space velocity of $0.2h^{-1}$ for 30 days showed only 5% loss of initial activity.

• Animal Systematics, Evolution and Diversity
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• v.16 no.2
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• pp.227-237
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• 2000

This study deals with 8 species in 3 families of Collembola including 6 new records of Korea. They are Oudemansia esakii (Kinoshita, 1932), Axelsonia littoralis (Moniez, 1890), Sinella (S. str.) straminea (Folsom, 1899), Sinella (S. str.) coeca (Schott, 1899), Entomobrya marginata (Tullberg, 1871), and Willowsia bimaculata (Borner, 1909). Among them Oudemonsia esakii, Axelsonia littoralis and Entomobrya marginata are halophilous species. Sinella straminea and Sinella coeca were collected from nests of ants comprising the first report of myrmecophilous Collembola from Korea. The Collembolan fauna of Korea is accordingly listed as 203 species in 10 families.

• Journal of Microbiology
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• v.41 no.1
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• pp.16-21
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• 2003

We have collected eight high-level streptomycin-resistant strains of Pseudomonas marginalis and P. syringae pv. actinidiae which were isolated from kiwifruit orchards in Korea and Japan, The molecular mechanisms of resistance were investigated by the PCR, susceptibility tests, and nucleotide sequence analysis. Of the eight high-level streptomycin-resistant strains, four harbored strA-strB genes, which encode streptomycin-inactivating enzymes. While the three Korean strains of R marginalis did not have plasmid and carried the resistant genes in the chromosomes, the Japanese strain of P. syringae pv. actinidiae had a plasmid containing strA-strB genes. The myomycin susceptibility test demonstrated that the high-level resistance to streptomycin of the remaining four strains is associated with mutations in the rpsL gene. Nucleotide sequence analyses revealed that they contain a single base-pair mutation in codon 43 of their rpsL gene.

• Molecules and Cells
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• v.42 no.1
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• pp.28-35
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• 2019

Animals need to be able to alter their developmental and behavioral programs in response to changing environmental conditions. This developmental and behavioral plasticity is mainly mediated by changes in gene expression. The knowledge of the mechanisms by which environmental signals are transduced and integrated to modulate changes in sensory gene expression is limited. Exposure to ascaroside pheromone has been reported to alter the expression of a subset of putative G protein-coupled chemosensory receptor genes in the ASI chemosensory neurons of C. elegans (Kim et al., 2009; Nolan et al., 2002; Peckol et al., 1999). Here we show that ascaroside pheromone reversibly represses expression of the str-3 chemoreceptor gene in the ASI neurons. Repression of str-3 expression can be initiated only at the L1 stage, but expression is restored upon removal of ascarosides at any developmental stage. Pheromone receptors including SRBC-64/66 and SRG-36/37 are required for str-3 repression. Moreover, pheromone-mediated str-3 repression is mediated by FLP-18 neuropeptide signaling via the NPR-1 neuropeptide receptor. These results suggest that environmental signals regulate chemosensory gene expression together with internal neuropeptide signals which, in turn, modulate behavior.

• Journal of IKEEE
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• v.24 no.4
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• pp.1086-1092
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• 2020

An autonomous agent for real world should be able to recognize text in scenes. With the advancement of deep learning, various DNN models have been utilized for transformation, feature extraction, and predictions. However, the existing state-of-the art STR (Scene Text Recognition) engines do not achieve the performance required for real world applications. In this paper, we introduce a performance-improvement method through an add-on composed of an OCR (Optical Character Recognition) engine and a classifier for STR engines. On instances from IC13 and IC15 datasets which a STR engine failed to recognize, our method recognizes 10.92% of unrecognized characters.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.2
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• pp.121-131
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• 2022

The goal of the study was to see how quality management (QMA), strategy (STR), and innovative capability (INC) influence the competitive advantage of a Thai information communication technology (ICT) firm (COA). The researchers collected 431 surveys from Thailand's owners and managers employed in ICT enterprises from the beginning of June 2021 to the end of September 2021using diverse sample strategies. A questionnaire with an index of item-objective congruence (IOC) value of 0.60-1.00 and a reliability value of 0.92-0.96 was used as the research tool. Participants in the survey were requested to fill out a seven-level opinion survey posted on Google Forms. A latent variable structural equation model (SEM) path analysis using LISREL 9.1 was used for the four latent variables, 31 manifest variables, and the five hypotheses testing. The analysis showed that all three causal variables positively affected COA, which had a total effect (TE) R² value = 80% when combined with the other latent variables. Moreover, the values for the latent variables when ranked by total effect (TE) were STR, QMA, and INC with TE values of 0.95, 0.89, and 0.25, respectively. Finally, there were very strong influences from COA to STR (0.95), INC to QMA (0.86), and STR to QMA (0.71).

• Journal of Sasang Constitutional Medicine
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• v.11 no.1
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• pp.169-183
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• 1999

This study was carried out for the objectification and clinical application of the Sasang Constitutional Medicine(四象醫學). So, the Taeum Soyang, Soum groups were classified by diagnostic rules of Sasang constitution, and investigated by Amp-FLP method far genetic difference. The Amp-FLP was one of the most frequently used human genetic analysis methods which adopts STR typing. In this study, 100 genomic DNA samples of Taeum, Soyang, Soum constitution group were analysed by Amp-FLP method. Allele frequencies of four tetrameric short tandem repeat(STR) loci(TPOX, LPL, D21744, and D13S317) were determined in Taeum, Soyang, Soum groups. The heterozygosities and the polymorphism information content(PIC) values of forur STR loci were 0.812 and 0.789 in D3S1744, 0.811 in D13S317, 0.466 and 0.417 in LPL, and 0.625 and 0.561 in TPOX, respectively. The allele distribution of four STR loci was statistically evaluated. It was found out that the allele distribution of four STR loci was not significantly different among different constitutions. But all loci were found to be highly polymorphic in Taeum, Soyang, Soum groups. It was found out in this study that Taeum, Soum groups are genetically more related each other than they are related to Soyang groups.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.2
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• pp.103-110
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• 2017

In this paper, it is covered in detail the process of generating structural alternatives with geometry change and its optimization by StrAuto. The main roof structure of the Exhibition Center is modelled parametrically and the optimal alt is derived by observing volume changes according to geometry change of main roof truss. Existing studies performed optimization process through sections and properties due to the limitations of shape change, but this study have meaning of performing the optimization with geometry changes which is the most critical skills of StrAuto. By the process of securing a sufficient margin by geometry changes and reducing volume with the optimization of sections, despite of a partial optimization of large space structure, it could be reduced by 11.7% of the total volume.

• Korean Journal of Agricultural Science
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• v.24 no.2
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• pp.156-169
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• 1997

This experiment was carried out to exame the effects of sweeteners, sucrose(2.0~10.0%), aspartame(0.01~0.05%) and oligosaccharide(3.6~11.6%) on the fermentation of yoghurts by single or mixed culture (Lact. bulgaricus and Str. thermophilus). The acidity, pH and number of lactic acid bacteria in yoghurts added different level of sweeteners were examined by the fermentation time. The results were summarized as follows; 1. The acidity increased and the pH decreased more rapidly by 4.0% sucrose and 5.6% oligosaccharide. By the addition of 8.0% sucrose and 9.6% oligosaccharide the acidity and pH of yoghurts were changed less significantly. 2. The number of Lact. bulgaricus and Str. thermophilus increased more rapidly by 4.0% sucrose and 5.6% oligosaccharide and increased slowly above those levels. 3. Aspartame as a sweeteners did not affect on the acidity, pH and number of lactic acid bacteria in yoghurts. 4. The number of lactic acid bacteria, acidity and pH in yoghurts added sucrose and oligosaccharide were affected more by single culture than by mixed culture.