• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.6
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• pp.137-148
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• 2008

There have been many cases of using wetlands as an alternative in controlling stormwater, treating mining leachate, and agricultural discharge, and so on, recently. The reality is, however, that the wetlands are not properly applicable because of the lack of enough longterm data for wetlands due to the difficulty of long-term monitoring. Therefore, this study tries to analyze the storage of Upo, Mokpo, Sajipo, and Jjokjibeul in Topyeong watershed using SWAT(Soil and Water Assessment Tool) model, one of the long-term runoff hydrologic model, for the purpose of generating the long-term data and analyzing the hydrologic behavior of wetlands based on the generated data. Also, the changes in runoff at the outlet are analyzed after applying the simulation of constructing washland in Topyeong watershed and the storage in Upo is analyzed. The result shows that the runoff at the outlet of the watershed is decreased in rainy season from July to August and increased in dry season from December to February. In addition, the analysis of Upo storage concludes that Upo can be influenced by the construction of the washland. The duration curve of washland is then analyzed in order to evaluate the wetland's sustainability in terms of washland and it appears that the runoff of washland is simulated to be less than that of the existing wetland. Moreover, runoffs of some washlands are simulated to be less even in wet season. These results lead to the fact that there should be further hydrologic management for constructed washland. Then, the changes in loads (TN and TP) because of constructing washland are analyzed. The result shows that the loads are reduced because of the construction. Also, the changes in loads due to the construction of buffer strips are analyzed to compare the load reductions caused by a washland. Finally, REMM model, a riparian management model, is applied to overcome the hydrologic ambiguousness of SWAT model, and then, the SWAT model results are compared to those of REMM.

• Journal of Food Hygiene and Safety
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• v.32 no.3
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• pp.206-210
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• 2017

This study was performed to develope mathematical models for predicting growth kinetics of Staphylococcus aureus in the processed meat product, pyeonyuk. Growth patterns of S. aureus in pyeonyuk were determined at the storage temperatures of 4, 10, 20, and $37^{\circ}C$ respectively. The number of S. aureus in pyeonyuk increased at all the storage temperatures. The maximum specific growth rate (${\mu}_{max}$) and lag phase duration (LPD) values were calculated by Baranyi model. The ${\mu}_{max}$ values went up, while the LPD values decreased as the storage temperature increased from $4^{\circ}C$ to $37^{\circ}C$. Square root model and polynomial model were used to develop the secondary models for ${\mu}_{max}$ and LPD, respectively. Root Mean Square Error (RMSE) was used to evaluate the developed model and the fitness was determind to be 0.42. Therefore the developed predictive model was useful to predict the growth of S. aureus in pyeonyuk and it will help to prevent food-born disease by expanding for microbial sanitary management guide.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.542-549
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• 2015

This work was carried out to evaluate the effects of preharvest 1-methylcyclopropene (1-MCP, $Harvista^{TM}$) and postharvest 1-MCP ($SmartFresh^{TM}$) treatments on the fruit quality attributes of cold-stored 'Fuji' ( Malus domestica Borkh.) apples. Fruits were exposed to 0, 95, 125, or $250 mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 3, 2, 1 weeks before harvest (WBH), and treated with 0 or $1{\mu}{\cdot}L^{-1}$ $SmartFresh^{TM}$ at harvest. Fruit was then stored for up to 180 days at $0{\pm}1^{\circ}C$. Fruit fresh weight, Hunter's value a, internal ethylene concentration (IEC), flesh firmness, titratable acidity (TA), and soluble solids content (SSC) in fruit treated with $Harvista^{TM}$ were not different from those of control fruit at harvest. During cold storage, flesh firmness and TA were higher in fruit treated with $250mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 2 and 3 WBH than in control fruit. IEC was 5.5-10.0% lower in fruit treated with $250mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 2 and 3 WBH compared with control fruit as storage duration progressed, while SSC was not affected. Furthermore, flesh firmness, TA, and IEC were affected neither by $Harvista^{TM}$ nor $Harvista^{TM}+SmartFresh^{TM}$ treatments, compared with those fruit quality attributes at harvest. The correlation maps indicated that IEC was negatively correlated with firmness and TA, regardless of $Harvista^{TM}$ application levels. In addition, positive correlations between fruit quality attributes were detected in treatments with $250mg{\cdot}L^{-1}$ $Harvista^{TM}+SmartFresh^{TM}$. Therefore, the results suggest that with a single application of $SmartFresh^{TM}$, a higher level of $Harvista^{TM}$ application would help in retention of fruit quality attributes during cold storage.

• Clinical and Experimental Reproductive Medicine
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• v.27 no.4
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• pp.367-372
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• 2000

Objective: This study was performed to evaluate whether vitrification method could be used for the cryopreservation of human blastocysts derived from IVF program. Methods: Surplus embryos were obtained from consented IVF patients. Controlled ovarian hyperstimulation was done with midluteal GnRH agonist, gonadotropin and hCG. After oocyte retrieval and insemination, fresh embryo transfer was done at $4{\sim}8$ cell stage. The surplus embryos after ET were cultured in blastocyst medium up to 6 days after oocyte retrieval. Obtained blastocysts were cryopreserved with our vitrification method. Blastocysts were exposed to 1.5 Methylene glycol (EG) in phosphate buffered saline (PBS) for 2.5 minutes, followed by 5.5 M EG plus 1 M sucrose for 20 seconds. Then 1 to 3 blastocysts were mounted on electron microscope (EM) grid and the grid was plunged into liquid nitrogen for storage. For thawing, blastocyst-containing EM grids were sequentially transferred in 1.0 M, 0.5 M, 0.25 M, 0.125 M and 0 M sucrose solution at the intervals of2.5 minutes. And blastocysts were cultured for about 6 hours and only re-expanded blastocysts were transferred to uterus of the patients on 4 to 5 days after ovulation in natural cycle or on 18 to 19 day of artificial cycle. Results: From Oct. 1998 to Jul. 1999, 34 patients were agreed to participate in this study. The mean age and duration of infertility of the patients were 31.6 years and 4.1 years, respectively. Among 34 cycles. replacements could be done in 20 cycles (58.8%). A total 93 blastocysts were thawed and 48 (51.6%) of them survived. Thirty-eight blastocysts, mean 1.9 embryos per patient, were transferred, resulting in 5 clinical pregnancies which consisted of 1 triplet, 2 sets of twins and 2 singleton pregnancies. The pregnancy rate per transfer was 25% and implantation rate was 23.6%. Five patients delivered 7 healthy babies including 2 sets of twins at term. Conclusion: Successful pregnancies and deliveries were established after transfer of vitrified human blastocysts. Vitrification using ethylene glycol as cryoprotectant and electron microscope grid is a rapid and simple method that can be effectively applied for the cryopreservation of human blastocysts.

• Journal of Korea Water Resources Association
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• v.42 no.12
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• pp.1069-1077
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• 2009

Lately, it is an important concern in water resources research to maintain a stable water supply according to a future climate change and an increase in water use. In Han-River basin, approximately 10 % of water resources that is provided the capital region (Gyeonggi, Seoul etc.) has been reduced as a consequence of the construction of Imnam Dam (storage volume: 27 billion $m^3$) located in the upper Hwacheon Dam upstream area. Therefore, streamflows have decreased in Bukhangang basin, but it could not be evaluated quantitatively. In this study, SWAT-K which is the physically based long-term runoff simulation model, was used in order to evaluate the effect of Imnam Dam on the reduced inflow to Hwacheon Dam according to the change of hydrological condition in the upstream area of Hwacheon Dam. For the model input data of North Korea area, meteorological data of GTS (Global Telecommunication System) were used, and soil maps by FAO/UNESCO (2003) were applied. Temporal variations of water resources is investigated with comparison of observed and simulated inflows at Hawcheon Dam site. Also, annual, monthly, seasonal decreases in water resources were evaluated using the flow duration analysis of simulated streamflows with or without Imnam dam.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.194-200
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• 2014

Skin ridge (SR) is a irregular line shape that stand out in epidermal layer on sweet potato (Ipomoea batatas Lam.). SR reduced marketable value of storage root in sweet potato. It would be occurred by accumulation of starch in epidermal layer. SR rate was more highly increased in early planting. SR rate was under 3% in 7 varieties 'Geonpoongmi', 'Yeonhwangmi', 'Geonmi' etc and the rate of two varieties 'Healthymi', 'Borami' were up to 30%. SR rate was positively correlated with vine weight. SR rate was the highest in 120 days after planting. As leaf area index was reduced to 3, 4, 5 and 6 (non-topping) by topping, it was hard to fine SR occurrence in topping treated sweet potato. Topping treatment, however, caused decrease in yield overall. Therefore, topping treatment for decreasing of SR was not recommended in agricultural practice of sweet potato.

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.133-139
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• 2011

In this study, we determined the changes in microbial numbers in solar salts according to the manufacturing process and storage duration. The salt samples were harvested from salt farms in Shinan (area 2) and Yeonggwang (area 1). They were serially diluted ten-fold and then placed on 4 kinds of cultivable media (mannitol salt agar, eosin methylene blue, plate count agar, and trypticase soy agar). After incubation, we obtained 62 halophilic isolates from the salt samples. Coliform and general bacteria were not detected in all salt samples. By 16S rRNA sequencing analysis, we found 12 kinds of halophilic bacteria belonging to the genera Halobacillus, Halomonas, Bacillus, Idiomarina, Marinobacter, Pseudoalteromonas, Vibrio, Salinivibrio, Virgibacillus, Alteromonas, Staphylococcus and some un-known stains. In our study, we discovered two novel species that have a 16S rDNA sequence similarity below 97%.

• Journal of Acupuncture Research
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• v.32 no.4
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• pp.37-45
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• 2015

Objectives : The main aim of this study is to evaluate changes in the microbiological safety of pharmacopuncture exposed to room temperature for an elapsed period of time. Methods : The four most frequently used pharmacopuncture products were stored in syringes at room temperature at three different hospitals for 24 hours and 48 hours respectively, and they were compared with pharmacopuncture products stored in vials through a sterility and microbial limited test. Results : Storage forms and duration of exposure to room temperature did not show significant difference in bacterial or fungal contamination, which was confirmed by the sterility and microbial limited test. Conclusions : Pharmacopuncture products stored in syringes at room temperature for 24 hours and 48 hours demonstrated their safety in terms of lack of microbiological contamination.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.8 no.4
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• pp.41-48
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• 1988

A model for predicting the probability of liquefaction induced by the ocean storm-wave is developed. Many uncertainties are encountered in the analysis of liquefaction potential for the foundation of offshore structures : (1) the storm-wave duration, (2) the effect of reconsolidation, (3) the effect of patial drainage, etc. These uncertainties are formulated in probabilistic terms and used to assess the risk of liquefaction for a given offshore site. The model developed is applied to the Ekofisk oil storage tank in the North Sea installed in 1973. Reasonable comparison is obtained between the probabilities of liquefaction obtained and the results of deterministic models or the field observations. Among the un certainties encountered, it is revealed that the effect of reconsolidation is the most critical factor. Since many problems are encountered in the deterministic models developed so far, the probabilistic model developed in this paper might be a resonable alternative tool and can be used in the design of new offshore structures.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.87-96
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• 1968

As the raw material of fish paste, the experiment on shark meats was conducted from July to December 1967 at the Fisheries Research and Development Agency, Pusan, Korea. The result and the conclusion obtained by the experiment are as follows. 1. Much salt-soluble protein was found in the shark meat comparing with to the other fish. 2. Deodorization of shark meat, using less than $0.05\%$ of acetic acid was most preferable for the elasticity and flexibility of its product. 3. Keeping raw material at low temperature was necessary in maintaining its elasticity. 4. In setting shark fish paste, keeping the raw material at $35-40^{\circ}C$ for 40-70 minutes was the most desirable condition. 5. The best temperature and the duration of heating were 40-60 minutes at $89-90^{\circ}C$, Meanwhile, heating at high temperature was preferable for the storage for a long period, but the elasticity and quality has decreased a little accordingly. 6. For the increase of its palatability, adding $30\%$ of yellow corvenia meat or $20\%$ sea eel meat were more preferable. 7. Pre ervation of the product for 20 days at $20^{\circ}C$ or 50 days at $5^{\circ}C$ was possible by adding $0.2\%$ potassium sorbinate to the product as antiseptic.