• Title/Summary/Keyword: Stool culture

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Study on Origin and Creative Design of Korean Folding Stool (한국 호상(胡床)의 시원(始原)과 조형에 관한 연구)

  • Hong, Sun Aa
    • Journal of the Korea Furniture Society
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    • v.24 no.2
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    • pp.156-182
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    • 2013
  • In this paper, the origin, development and features of a Korean folding stool are studied. A folding stool is a piece of collapsible seating furniture with its legs crossed in the form of the letter "X" and no back attached. In China, the folding stool was introduced from the West around the second century A.D. The folding stool of Korea was influenced by that of China. It is known from the records that the king used the folding stool from the Three Kingdom period. During the Koryo period, Chosun period and the modern period, the folding stool was used to show the authority of the king and nobleman. The folding stool was also used at outside places such as a hunting ground, a ceremonial walk, a battlefield, a garden etc. due to its portableness. Buddha worship policy of Koryo period supported the usage of the folding stool for shanding (禪定) by ascetics of Buddhist temple, or aristocrats. In a contemporary period, the folding stool is continuously produced and used as one kind of a folding chair due to its functionality, movability, storability and efficient space utilization. This research will serve as a momentum to investigate the origin and development of the East Asian seating furniture culture, and contribute to the further study and manufacturing of Korean seating furniture.

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In Vitro Propagation Using Stool Shoots of Mature Betula platyphylla var. japonica (자작나무 성숙목의 근주맹아를 이용한 기내증식)

  • Moon, H.K.;Youn, Y.;Hyun, Y.I.;Lee, S.K.
    • Journal of Korean Society of Forest Science
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    • v.80 no.4
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    • pp.416-419
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    • 1991
  • Effective micropropagation was achieved by axillary bud culture from stool shoots of 15-year-old Betula platyphylla var. japonica. Shoot development and proliferation from the explants were successful on WPM supplemented with 0.5 or 1.0mg/l BAP. All the regenerated shoots rooted when transfered to GD medium containing 0.2mg/l IBA. After transplaning to soil more than 95% of the plantlets survived and showed normal growth. The results demonstrate that masspropagation of selected mature trees is feasible using tissue culture technique.

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Diagnosis of Clostridium difficile infection in patients with hospital-acquired diarrhea

  • Ibrahim Afifi, Salwa Selim;Gomaa, Fatma Alzahraa M.;Fathi, Lamia Fouad;Rasslan, Fatma Salah;Hamdy, Ahmed Mohamed
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.214-221
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    • 2018
  • Clostridium difficile infection (CDI) is a rapidly emerging infection that may have devastating consequences. Prompt and accurate diagnosis is crucial for management and control. The aim of this study was to determine the incidence of C. difficile associated diarrhea among hospitalized patients, and to compare different diagnostic laboratory methods for detection of toxin producing strains in clinical specimens. The study was conducted at a university hospital in Cairo during the period from May 2013 till June 2015. Subjects were under antibiotic therapy and presented with hospital-acquired diarrhea. Four hundred and sixty-five stool specimens were processed by different microbiological methods. C. difficile was recovered in culture in 51 of stool specimens. Of these, 86.3% to 98% were positive for toxin production by 2 different methods. This study showed that antibiotic intake is the major risk factor for development of hospital-acquired diarrhea. We evaluated different microbiological methods for diagnosis of C. difficile. We recommend the use of toxigenic culture as a gold standard for microbiological diagnosis of C. difficile.

Clostridium Difficile Colitis in Childhood: Associated Antibiotics (소아 Clostridium Difficile 장염과 관련된 항생제에 대한 연구)

  • Kim, Byoung-Chan;Yang, Hye-Ran;Jeong, Su-Jin;Lee, Kyung-Hoon;Kim, Jeong-Eun;Ko, Jae-Sung;Kim, Eui-Chong;Seo, Jeong-Kee
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.5 no.2
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    • pp.143-149
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    • 2002
  • Purpose: The following study was performed to reveal the relationship between Clostridium difficile colitis in childhood and associated antibiotics. Methods: From January 2000 to June 2002 at Seoul National University Children's Hospital, 85 symptomatic pediatric patients who showed positive stool culture for Clostridium difficile were included. The implicated antibiotics within 2 months before stool culture were analyzed. Of the 85 patients, there were 50 males and 35 females, and their average age was 2.5 years. Results: There was a history of implicated antibiotics within 2 months in 55 cases (67%). Forty-three patients (78%) of them showed Clostridium difficile in stool culture during antibiotics treatment. The time interval between the initiation of antibiotics and stool culture ranged from one day to 7 weeks (mean 10 days) in these patients. In the remaining 12 patients, Clostridium difficile was detected after the discontinuation of antibiotics. The time interval between the discontinuation and stool culture ranged from one day to 7 weeks (mean 12 days). The associated antibiotics were cefotaxime (20 cases), amikacin(15 cases), ampicillin (13 cases), cefazolin (8 cases), vancomycin (8 cases), etc. In 31 cases, more than one antibiotics were prescribed. Conclusion: When diarrhea occurred in young children during antibiotic usage or with a past history of recent antibiotic usage, Clostridium difficile should be investigated as a cause of diarrhea for proper management.

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A Case Report of a Stroke Patient with Vancomycin-Resistant Enterococci Colonization Treated with Korean Medicine, Especially Gami-sipjeondaebo-tang (반코마이신 내성 장알균(Vancomycin Resistant Enterococci) 집락이 검출된 뇌졸중 환자에 대한 가미십전대보탕의 효과 1례)

  • Seo, Hee-jeong;Bae, Go-eun;Choi, Jin-yong;Shim, So-hyun;Seo, Hyung-bum;Han, Chang-woo;Kim, So-yeon;Choi, Jun-yong;Park, Seong-ha;Yun, Young-ju;Lee, In;Kwon, Jung-nam;Hong, Jin-woo
    • The Journal of Internal Korean Medicine
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    • v.39 no.5
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    • pp.939-948
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    • 2018
  • Objectives: We treated a stroke patient with vancomycin-resistant enterococci (VRE) colonization using Gami-sipjeondaebo-tang. Methods: A patient diagnosed with intracranial hemorrhage with VRE colonization was treated with herbal medicine and acupuncture. We checked general conditions every day and performed stool VRE culture once a week. We evaluated the improvement of symptoms by change in VAS grade, general weakness, and stool VRE culture results. Results: After a treatment of Gami-sipjeondaebo-tang combined with acupuncture therapy, improvement of the general condition was observed. Also, VRE colonization was no longer detected in the stool culture. Conclusions: This case report proved the effect of Korean medicine for a stroke patient with VRE colonization, but further study is needed.

A case of Strongyloides stercoralis infection

  • Hong, Sung-Jong;Han, Joo-Hee
    • Parasites, Hosts and Diseases
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    • v.37 no.2
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    • pp.117-120
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    • 1999
  • Strongyloidiasis has been rdcognized as one of the life-threatening parasitic infections in the immunocompromised patients. We report an intestinal infection case of Strongyloides stercoralis in a 61-year-old man. Rhabditiform larvae were detected in the stool examination and developed to filariform larvae having a notched tail through the Harada-Mori filter paper culture.The patient received five courses of albendazole therapy but not cured of strongyloidiasis.

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Isolation Rate of Campylobacter fetus subsp. jejuni from Enteritis Patients (장염환자에서의 Campylobacter fetus subsp. jejuni 분리율)

  • Chong, Yun-Sop;Yi, Kui-Nyung;Lee, Sam-Uel Y.
    • The Journal of the Korean Society for Microbiology
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    • v.17 no.1
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    • pp.43-47
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    • 1982
  • C. fetus suhsp. jejuni has been reported to be an important enteric pathogen in many parts of the world. Although the infection has been reported in Korea, the incidence is not known. In this study the results of stool culture during the period of August 1981 to July 1982 at Yonsei Medical Center was analyzed and the following results were obtained. 1. C. fetus subsp. jejuni was isolated from 0.8% of stool specimens. The isolation rate was lower than that of salmonella(3.3%) and shigella(7.1%). The isolation was most frequent in June and from $\leq$15-year-old patients. 2. All of the isolates from the patients were susceptible to chloramphenicol and erythromycin. It was noteworthy that 4 isolates were resistant to all of the aminoglycosides, i.e., amikacin, gen tamicin, kanamycin and tobramycin. 3. We also isolated C. fetus subsp. jejuni from chicken. When the susceptibility of the isolates was compared to that of the isolates from human the former were less susceptible to erythromycin(34.1%) and tetracycline(38.6%).

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Trends of Antimicrobial Susceptibility Test for Bacterias Isolated from Blood, Urine, Stool, and Cerebrospinal Fluid(1997~2001) (혈액 및 일반 세균배양에서 검출된 균종과 항균제 감수성 추이(1997~2001))

  • Hong, Mi Ae;Oh, Kyung Chang;Ahn, Seng In;Kim, Bong Rim;Kim, Yun Ho;Kim, Sung Seop;Chang, Jin Keun;Jeun, Kyoung So;Cha, Sung Ho
    • Pediatric Infection and Vaccine
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    • v.10 no.2
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    • pp.167-177
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    • 2003
  • Purpose : To know the trends of antimicrobial susceptibility is critical for antimicrobial treatment. We studied the organisms isolated from blood, urine, stool, and cerebrospinal fluid from 1997 to 2001 to reveal the trends of their antimicrobial susceptibility. Methods : We conducted a retrospective study with isolates obtained from 0~18 year old outpatients and inpatients from 1997 to 2001 at Department of Pediatrics, Hanil general hospital. We gathered the data through the laboratory test files and the origin of microorganisms cultured from blood, urine, stool and cerebrospinal fluid and their antimicrobial susceptibility. Results : Microorganisms were isolated from 226(3.3%) out of 6,974 blood cultures, 365 (8.0%) out of 4,549 urine cultures, 50(1.9%) out of 2,593 stool cultures and 9(1.4%) in 655 cerebrospinal fluid cultures. The most frequently isolated organisms from blood cultures was Staphylococcus epidermidis(33.5%) which was followed by Staphylococcus aureus(19.7%), Escherichia coli(13.8%), and Burkholderia cepacia(9.0%). Among the urine cultures, E. coli was the most common(74.7%) which was followed by Group D Enterococcus(11.3%), Klebsiella pneumoniae(7.1%) and Proteus mirabilis(2.5%). The positive stool cultures all yield Salmonella species. Group D Salmonella was obtained most frequently. Among the positive cerebrospinal fluid cultures, Group B Streptococcus was isolated most frequently. Among the 40 cases of S. aureus in blood cultures, 27 cases were methicillin-resistant. The rates of susceptibility for amikacin, ceftizoxime and ceftriaxone of E. coli isolated from blood cultures were 80%, 100% and 60% in 1997 and 60%, 80% and 60% in 2001. The rates of susceptibility for amikacin, ceftizoxime and ceftriaxone of K. pnumoniae isolated from urine cultures. were 80%, 100% and 80% in 1997 and 50%, 83% and 50% in 2001 Enterococcus was isolated from 6.7% to 15.8% and vancomycin-resistant Enterococcus was observed in 17% of Group D Enterococcus isolated from urine cultures. The rates of susceptibility for amikacin, ceftizoxime and ceftriaxone of Group D Salmonella were 96%, 96% and 92% during the study period. Conclusion : Among the blood cultures S. epidermidis, S. aureus, E. coli and B. cepacia were isolated in order of frequency and among the urine cultures E. coli, Group D Enterococcus, K. pneumoniae and P. mirabilis were isolated in order of frequency. During the study period there was no big difference in major organisms isolated from blood and urine. The methicillin-resistant S. aureus was observed in 67% of S. aureus isolated from blood cultures but vancomycin-reistant S. aureus or vancomycin intermediate resistant S. aureus was not observed. The rates of susceptibility to amikacin and the third generation cephalosporin of E. coli isolated from blood cultures and K. pneumoniae from urine cultures have decreased. The isolation rates of Group D Enterococcus and vancomycin resistant Enterococcus have increased.

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Salmonellosis in children: Analysis of 72 Salmonella-positive culture cases during the last 10 years (소아의 살모넬라 감염증: 최근 10년동안 살모넬라 배양 양성인 72례에 대한 분석)

  • Noh, Sung Hoon;Yu, Ka Young;Kim, Jung Soo;Hwang, Pyoung Han;Jo, Dae Sun
    • Clinical and Experimental Pediatrics
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    • v.52 no.7
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    • pp.791-797
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    • 2009
  • Purpose : We aimed to investigate the clinical manifestations of and antibiotic resistance in culture-proven childhood salmonellosis. Methods : Clinical manifestations and laboratory data of and antibiotic use in subjects with culture-proven childhood salmonellosis, who were treated at the Chonbuk National University Hospital between September 1998 and August 2008, were analyzed retrospectively. Patients with underlying diseases or concomitant illnesses were excluded. Results : We assessed a total of 72 patients. There were 68 stool culture-positive cases, 7 blood culture-positive cases, and 3 both stool culture- and blood culture-positive cases. Salmonella group D was the most frequent pathogen in stool (63.9%) and blood (71.4%) cultures. Salmonella typhi was isolated in 1 case. Of the 72 patients, 45 (62.5%) were male children, of which 29 (40.3%) were aged <3 years. The patients most commonly presented with diarrhea (90.2%) and fever (83.3%). Leukocytosis (leukocyte count, >$15,000/{\mu}L$) and leukopenia (leukocyte count, <$4,000/{\mu}L$) were detected in 8.3% and 5.6% of the patients, respectively. Elevated serum C-reactive protein concentration (>5 mg/dL) and erythrocyte sedimentation rate (>20 mm/h) were observed in 88.9% and 58.3% of the patients, respectively. Fifty-two (85.2%) of 61 patients who had undergone antibiotic treatment received a third-generation cephalosporin as definitive antibiotic therapy. Multidrug resistance rate was 40.0 % in the first 5 years of the study and 71.4% in the last 5 years. No fatalities occurred in this series. Conclusion : Children with culture-proven salmonellosis showed relatively benign clinical outcomes. Appropriate antibiotic treatment of <2 weeks is probably adequate for those without a suppurative focus of infection. The incidence of antibiotic resistant isolates was recently seen to increase.

A Modified Formalin-Ether Concentration Technique for Diagnosis of Human Strongyloidiasis

  • Anamnart, Witthaya;Intapan, Pewpan M.;Maleewong, Wanchai
    • Parasites, Hosts and Diseases
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    • v.51 no.6
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    • pp.743-745
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    • 2013
  • We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.