• The Korean Journal of Physiology and Pharmacology
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• v.19 no.1
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• pp.35-42
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• 2015

In cardiovascular disorders, understanding of endothelial cell (EC) function is essential to elucidate the disease mechanism. Although the mouse model has many advantages for in vivo and in vitro research, efficient procedures for the isolation and propagation of primary mouse EC have been problematic. We describe a high yield process for isolation and in vitro culture of primary EC from mouse arteries (aorta, braches of superior mesenteric artery, and cerebral arteries from the circle of Willis). Mouse arteries were carefully dissected without damage under a light microscope, and small pieces of the vessels were transferred on/in a Matrigel matrix enriched with endothelial growth supplement. Primary cells that proliferated in Matrigel were propagated in advanced DMEM with fetal calf serum or platelet-derived serum, EC growth supplement, and heparin. To improve the purity of the cell culture, we applied shearing stress and anti-fibroblast antibody. EC were characterized by a monolayer cobble stone appearance, positive staining with acetylated low density lipoprotein labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate, RT-PCR using primers for von-Willebrand factor, and determination of the protein level endothelial nitric oxide synthase. Our simple, efficient method would facilitate in vitro functional investigations of EC from mouse vessels.

• Journal of Conservation Science
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• v.23
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• pp.11-24
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• 2008

A physical characteristics and chemical compositions change by weathering on the granite were examined for the conservation treatment of the Mireuksaji temple stone pagoda. The natural weathered granite was collected from the Mt. Mireuk, and divided into the classification standards based on weathering degrees and strength measured by rock-test hammer. The results from comparison of the strength measured by undestructive rock-test hammer and the strength values converted from ultrasonic velocity showed that each strength measurement value was proportionate. The water absorption of the sample was 1.68 to 0.20%. The F-type of fresh rock was not naturally saturated and the WW-type was naturally saturated but took quite a long time. The water absorption was increased gradually in order of SW-type, the MW-type and the HW-type according to weathering condition. The CW-type samples showed the highest water absorption among the weathered classification samples. Through dyeing test, it was found out that only the feldspar was dyed out of the F-type and the WW-type. The SW-type and the MW-type were distinguished by the fact that plagioclase being dyed. And dyed area was expanded to quartz crack in HW-type and CW-type. Physical change by weathering of the rock-forming minerals could be classified with 3 grades. Through the XRD analysis, albite among the rock-forming mineral showed remarkable decrease. SEM-EDX analysis of the component change in the rock-forming minerals such as biotite, plagioclase, and orthoclase, showed that in case of highly-weathered grade samples compared with fresh samples, contents of the $Al_2O_3$, $K_2O$, $Na_2O$ increase and CaO, MgO decrease in the biotite, the CaO, $K_2O$ increase and $Na_2O$ decrease in the plagioclase, the $Al_2O_3$ a little increase and $K_2O$, $Na_2O$ decrease in the orthoclase. The results of extracted cation analysis using the powder samples of each weathering grade, the CaO, $Na_2O$, $K_2O$ and MgO are highly chemical variations in rock forming minerals and positive variation show high in the weathering grade of the WW-type and CW-type. This research will be used as an importance data to establish a plan for conservation treatment of composed stone in the Mireuksaji temple stone pagoda.

• Journal of architectural history
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• v.25 no.5
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• pp.7-14
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• 2016

The architectural influence from the Yuan had impact on the Goryeo Dynasty in earnest during Yuan intervention period in the 14th century. The representative examples which were influenced by the Yuan architecture are the Eungjinjeon in Seongbulsa(成佛寺) temple, the ten-story stone pagoda of Gyeoncheonsa(敬天寺) temple site, the Bogwangjeon in Simwonsa(心源寺) temple, the Hoeamsa(檜巖寺) temple and so on. Notwithstanding the changes of relationship between two countries, it can be comprehended that there was a selective acceptance of the Yuan architectural peculiarities in Goryeo Dynasty. It means that the adoption of foreign culture in Korea has not been inevitable from the unilateral demand, but been autonomous by perceiving as the advanced culture. This tendency was maintained even though the government had been changed.

• Conservation and Restoration of Cultural Heritage
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• v.1 no.1
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• pp.39-48
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• 2012

The first twenty-five years of scientific study within Korea on Korean traditional ceramics has been characterized as a bridging effort to understand the rich field of artistic ceramic masterpieces on one hand with analytic results gained from mounds of broken shards and kiln wastes on the other. First shard pieces were collected directly from the waste mounds, but most of the analyzed shards were provided by art historians and museum staffs directly involved in systematic excavations. The scientific study is viewed as one of many complimentary ways in learning about the multi-faceted ceramics culture, ultimately connecting human spirits and endeavors from the past to the present to the future. About 1350 pieces of analyzed shards have been so far collected and organized according to the production location and time period. From the experimental results of the analysis, the compositional and microstructural characteristics of bodies and glazes have been deduced for many kiln sites of Goryeo and Joseon dynasties. Except for a few local kilns, porcelain stone was used as body material in both dynasties. The principle of mixing a clay component with a flux material was used in Korean glazes as was in China. The clay component different from body clay was often used early on. In Gangjin a porcelain material appropriate for whiteware body was mixed for celadon glaze, and in Joseon Gwangju kilns glaze stone was chief clay material. The use of wood ash persisted in Korea even in making buncheong glazes, but in Joseon whitewares burnt lime and eventually crushed lime were used as flux material.

• The Research Journal of the Costume Culture
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• v.18 no.5
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• pp.960-976
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• 2010

Even though Joseon Dynasty strictly identified between military and civil officials, the dress and its ornament have been studied only based on embroidery emblems that are correctly identified between military and civil officials. Thus, this study intends to research the features of the only military officials uniform, Cheollik, different from the civil officials's identifying them with other features shown from the records, unearthed relics, stone statue, Joseon-tongsinsa-haengnyeoldo(Illustration of Joseon Delegation to Japan), etc., and its results are as follows: First, for wearing examples of the military officials, from the facts that they had worn mainly ordinary clothes, etc. on announcement of military service examination. There was no difference between military and civil officials's clothes by Daejeonhusokrok and the other documents, and there was an assertion that the form of the military and civil officials's clothes should be different by the document, Hongjejeonseo, in the latter term of the Joseon Dynasty. It is presumed that length, width and shape of sleeves might have been different. Second, unearthed Cheollik of the military officials are mostly from the 16th~17th centuries and most of them have removable sleeves according to statistics. The skirt had an opening and one or two slits. Various textiles were used. Third, military official statues of high governor wear small caps and helmets. They wear Cheollik with the collars same as the collar of Bangryeong and Jikryeong, which is also called Dopji Cheollik. It would be considered as they wear the Bangryeong on top of the Cheollik. Fourth, militarly officials described on the Joseon-tongsinsa-haengnyeoldo(Illustration of Joseon Delegation to Japan) wear Cheollik, Rip, Donggae, Okro, Hongsadae, Hwando, Deungchae.

• The Research Journal of the Costume Culture
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• v.16 no.3
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• pp.461-474
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• 2008

The purpose of this study was to present a making method of jeans pattern with high fitness after deriving the appropriate shrinkage rate by material and washing process. Research Method of jeans pattern was presented after applying the optimized shrinkage rate. According to the result of the exterior evaluation of test jeans, all 6 jeans were rated high with scores close to 3.5. Following the evaluation of satisfaction of usage by physical movement, the highest ranking was in the order of walking with normal steps, back bending $90^{\circ}$, chair sitting, climbing stairs, and squatting. The shrinkage rate by physical area showed the highest score in the order of pants length, waist circumference, thigh circumference, knee circumference, hem circumference, hips circumference. In addition, the shrinkage rate was higher in warp direction than weft direction after washing finishing. As for the result of addition and reduction of pattern measurements by parts of jeans, waist circumference was $2.5{\sim}5.2cm$, hips circumference was $-1.8{\sim}2.8cm$, thigh circumference was $-1.3{\sim}2.0cm$ and knee circumference was $-1.0{\sim}1.7cm$. Also, hem circumference was $-1.0{\sim}1.8cm$ and pants length was $2.9{\sim}6.2cm$. That is, this results showed a wide range of addition and reduction according to material and washing finishing.

• Journal of the Korean Home Economics Association
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• v.33 no.5
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• pp.211-219
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• 1995

In this study of the Buddhist Stone Statues in Namsan in Kyungju. We have found out interesting facts as follow. This study is focused on the way the Buddhist Statues wear the robes. There are two basical types of wearing ; Tongkyun and Pyundanwookyun. In Silla, these types of wearing has been developed into its unique types based on the traditional culture. The stone Buddhist images show seven types of wearing based on the standard ones of Tongkyun and Pyundanwookyun. This variety in wearing rests on the various of Sungkari(Samghathi) and Bokgyenui(Samkaksika) and a chest sash which fixes Niwonsung(Nivasana). There are three cases inferred from the seven types of wearing the Buddha's robes : (1)Tonkyun type A: The only outer wear Sungkari(Samghathi) was put on as Tongkyun type. We can't find out any Bokgyenui(Samkaksika) or the chest sash of Niwonsung(Nivasana). (2)Tongkyun type B: Bokgyenui(Samkaksika) is put on the chest of Statue in Sungkari(Samghathi). (3)Tongkyun type C: This type is similar to Tonkyun type A, except that the end of the robe dangled down straight to the right side. (4)Tongkyun type D: Bokgyenui(Samkaksika) is bound with a chest sash. (5)Tongkyun type C: The robe is tied on the left shoulder as a cord and Bokgyenui(Samkaksika) is bound with a chest sash. (6)Pyundanwookyun type A: The robe dangled down from the left shoulder to the right armpit diagonally, so left shoulder is naked. (7)Pyundanwookyun type B: This type is similiar to Pyundanwookyun type A, but the end of Sungkari(Samghathi) is bound up to the left shoulder again. Considering above, the robe types of Buddha seem to have something to do with those of the Silla people: The chest sash is similar to the adjustment of Jeogori(the traditional jacket) of the Silla, and the typing cord in Buddha's robe corresponds to that of the traditional types.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.180-194
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• 1985

The author investigated the history of oyster culture in Korea through examining old books and reports dealing with the fishery. Oysters have been produced in the all coastal areas in Korea, especially the center areas of production were the Hwangupo (Hamkyongbuk-do), Younghungman (Hamkyongnam-do), the estuary of Nakdonggang (Kyongsangnam-do), Kwangyangman, Haechangman, the estuary of Yongsangang (Chollanam-do), Yonghoto (Hwanghae-do) and the estuary of Uprockgang, where the large size oyster (more than 40cm in shell height) inhabited in form of groups. It was called by nine different names in the old times, Gul, Seukhwa and Moryeo were the most common names. The oyster was used as 46 kinds of medicinal stuff. The habitat forms of natural oyster can be distinguished to two types; one is that oyster is exposed to the air during the period of low tide (A-type), another is that oyster is not (B-type). The first oyster culture by the old books in Korea was started about 530 years ago as the bottom culture method derived from the A-type of oyster. After long years, the hanging culture method might have been started from the hint of the habitat form of B-type. The oyster culture in Korea developed based on the habitat forms of natural oyster at the biginning of 1900s. After World War II, the Korean government strongly recommded the laver farmers to culture oyster as relief measures. At that time, the stone culture method was mainly adopted. The quantity of oyster production is now about $15{\times}10^6{\sim}19{\times}10^6\;\frac{M}{T}$, and the half of oyster production including seed oyster is exported to the foreign countries.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.1
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• pp.109-115
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• 2023

Interspecific hybridization is a beneficial strategy in aquaculture. In this study, diploid hybrids were produced through interspecies hybridization of flounder Platichthys bicoloratus ♀ × starry flounder P. stellatus ♂, which became commercial within a year. These hybrids exhibited an advantage in growth ability, with female hybrids growing twice as large as males at 11 months. Furthermore, hybrid flounders exhibited faster growth rates than starry flounders and reciprocal hybrids. The average growth rate of hybrids was 1.37 and 1.18 times greater than that of starry flounder and reciprocal hybrids at 5.5 months, respectively; Additionally, feed efficiency was 1.21 and 1.10 times higher than that of the experimental groups. The experiments were conducted at five temperatures to determine the optimal fry production method. Six weeks of culture resulted in a significant temperature effect, with maximum growth at 27℃. However, over 90% of the males occurred in the groups at temperatures above 24℃. Therefore, the optimum temperatures for survival, growth, condition factor and frequency of the best-sized fingerling at 13 weeks of age were estimated at 21℃. Thus, hybrid flounders are of great potential for use in the flounder aquaculture industry.

• Journal of the Korean Society of Food Culture
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• v.3 no.1
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• pp.7-16
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• 1988

An epigraph is an inscription mainly on a metal or stone monument, but some cases on bones or tortoise carapaces, ceramics, and coins. The '茶' inscriptions in 251 epigraphs in Korea are on 16 monuments, one tile, and one ceramic. By kingdom 5 belongs to shilla and 13 to Korea. The first '茶' inscription in on the Changsung-Tap of Borimsa Temple in Changhung-Kun, Chullanam-Do, which was established in 884 A.D. Religiously most of them are related to Buddism. Tea was most valued among valuables like gold, perlume, beads, etc. and so bestowed by kings. The study of epigraphs shows that our tea culture had most prospered diring the Kingdoms of Shilla and Korea.