• The Plant Pathology Journal
• /
• v.22 no.1
• /
• pp.21-27
• /
• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• Korean Journal of Medicinal Crop Science
• /
• v.3 no.3
• /
• pp.259-264
• /
• 1995

This study was carried out to find cultivation condition of peony in Euiseong district from 1994 to 1995. 1. Age distribution in cultivation of peony was from 30's to 50's and cultivated years was mainly from 5 years to 10 years, but there was more than 15 years 2. Cultivating area per house hold were 1300 pyeong average, there were moderate $400{\sim]1000$ pyeong and also those are occupied 22.6% more than 2000 pyeong. 3. The mothods of transplanting in Euiseong peony which Euiseong district was mostly divided peony but Yeongchen district used seedling stock. No. of sprout was mostly $3{\sim}4$ and planting time was mainly from Mid to Late of October. 4. The percentage of sterilization of soil and seedling stock was 77. 4%, in mulching by vinyl in tran­splanting 64. 5% in black vinyl and 25. 8% in white one. 5. Number of plant in peony planting was $3000{\sim}4000$ plant per l0a even more there was more than 8000 plant. 6. Chemical spraying time in peony field was 2 time in $1{\sim}2$ years cultivating, and $3{\sim}4$ time in $3{\sim}4$ years one. 7. Condition of fertilizer application in peony field was 40% in non-application, but $56.7{\sim}76.7%$ in 2 year to 3 years, and the time of fertilizer was mainly 3 time. 8. Drying time peony after havesting was from 12 to 24 hours by briquet stove and 24 hours by ma­chine of hot wind, also dry method of peony was mixed briquet stove and hot wind machine.

• The Plant Pathology Journal
• /
• v.21 no.4
• /
• pp.369-376
• /
• 2005

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.2
• /
• pp.81-85
• /
• 2001

The experiment was conducted to know the effect of plant growth regulators on axillary bud elongation from in vitro stem cutting and the possibility of virus-free stock production. Axillary buds were well elongated in 3/4 strength MS medium supplemented with 0.1 or 0.5 mg/1 BA and 0.05 mg/1 NAA. Transferred plantlets could be established well in vermiculite and peat moss mixture (3:1, v/v) compare to other mixtures. In virus indexing, all the varieties of mother plants were infected by GLRV Ⅲ. Infected percentages of the three varieties were ranged from 30% to 75%. But negative response was revealed against the other species of virus, GLRV Ⅰ, GFLV and ArMV. Plantlet of 'Schuyler' and 'Muscat of Alexandria', which were cultured in vitro, showed positive response against GLRV Ⅲ and infected percentage of the former was 37.5% but the latter, 12.5%. On the other hand, that of 'Campbell Early' negativiely responded against all the species of virus indexed.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.120-120
• /
• 2018

Intestinal microbiota is strongly connected to health of host. It has been reported that not only metabolic disease like diabetes and obesity, but psychological diseases are affected by composition of intestinal microbiota. To figure it out the importance of the composition and relationship between disease and microbiota, intensive researches have done with human and experimental animals. But, the composition of the intestinal microbiota could be affected by several factors such as experimental environments, feeding, water, and bedding. As a result, the data from each experimental group might be diverse. It also affects experiments about biological activities of plant materials. In this study, mouse intestinal bacteria were isolated from fresh feces and identified by 16S rRNA gene to use in biological activities of natural medicines. The fecal supernatant was anaerobically incubated at $37^{\circ}C$ for 48 hours. Colonies were picked up separately and incubated again in same condition to increase quantity to analyze and stock. The bacteria strains were listed up and could be used for many researches including biological activities of plant materials and change in composition of intestinal bacteria itself.

• The Plant Pathology Journal
• /
• v.18 no.5
• /
• pp.284-287
• /
• 2002

The antifungal activity of super reductive water (SRW) against plant pathogenic fungi was examined to extend its application to integrated pest management (IPM) for plant diseases. Diluted solutions ($\times$1/10, $\times$1/25, and $\times$1/50) of SRW inhibited fungal growth of kiwifruit soft rot pathogen, Diaporthe actinidiae, in a concentration dependent manner, When kiwifruits were inoculated on wounds with mycelium blocks, stock and diluted solutions successfully inhibited the disease development. In addition to the high pH of the SRW, fungistatic activity was also considered as the cause of the antifungal effect against the pathogen. Whereas conidial germination of Magnaporthe grisea was not affected by the diluted SRW solutions, appressorium formation was significantly inhibited in a concentration dependent manner, With little harmfulness to human health and environment SRW could be used to control plant pathogenic fungi, particularly appressorium-forming fungal pathogens.

• Journal of Plant Biotechnology
• /
• v.5 no.2
• /
• pp.101-105
• /
• 2003

To eradicate several viruses such as PVX, PVY, and PLRV which often cause considerable damages to the growth and yields of potatoes, several stems including shoot tips were excised from the potato plants grown for 50 days and electric shock was treated. Shoot tips excised from electric-shocked stems were transferred into the medium supplemented with antiviral compound, ribavirin to examine the combinatorial effect. When treated only with 20 mg/L ribavirin, PVX concentration in the regenerated plant-lets was slowly decreased as repeating sub-culture and finally, it took 32 weeks to reach completely PVX-free stock. With an electric shock treatment (10 mA electric current), all the replicates became free from PVY. However, PLRV was not completely eradicated from 94P70-4 and 93P29-3 lines even by treating with 10 mA electric shock. In this case, both electric shock and antiviral compound treatments in axillary buds from the stem segment were successful in eradicating viral contamination.

• Korean Journal of Organic Agriculture
• /
• v.21 no.2
• /
• pp.271-282
• /
• 2013

This experiments was conducted to investigate the influence on organic rice cultivation by continuous use of cow manure in the central region of Korea and investigated productivity of organic rice and feed value, stock carrying capacity of organic rice straw. In the results, application of chemical fertilizer showed higher one of 1st and 2nd year in fresh matter yield (15.77, 30.30ton/ha), dry matter yield (9.90, 13.89 ton/ha), dry matter yield of rice straw (4.88, 7.15ton/ha), dry matter weight of kernel per plant (29.81, 39.99g) and number of kernel per plant (1212.58, 1701units), but there were not significantly differences with each experimental plots in 3rd year. And harvest index showed higher one in application of chemical fertilizer (0.52, 0.48, 0.43) until 3 years. But their quantitative difference between application of chemical fertilizer and cow manure was gradually decreased each year. And the average value of $K_{CP}$ and $K_{TDN}$ showed higher one in application of chemical fertilizer (2.01, 2.94, 1.95 head/ha/yr) until 3 years. However, quantitative difference between chemical fertilizer and cow manure was gradually decreased each year. Especially, application of cow manure showed higher value of 3rd year in $K_{ME}$, but there was not significantly difference with each experimental plots. According to the results, continuous use of cow manure will be reduced their quantitative differences between chemical fertilizer as the year progress in the central region of Korea in terms of productivity of organic rice and feed value, stock carrying capacity of organic rice straw.

• Korean Journal of Environment and Ecology
• /
• v.22 no.4
• /
• pp.396-408
• /
• 2008

Gwangtan-myeon, Paju-si, Gyeonggi-do was classified as a 4 grade age-class deciduous tree forest, however as a result of vegetation survey, this site was found to consist of natural forest with deciduous trees, thus causing difficulty in judging which age class it belongs to. Subsequently, the necessity of drawing up a detailed stock map plan was raised. For this reason, this research was designed to propose a mapping method for a detailed stock map plan based on a detailed survey on actual vegetation, vegetation structure, and analysis data on tree rings. The detailed analysis of actual vegetation pattern showed that there exist 22 patterns of vegetation, in which the natural forest has 11 patterns, such as Quercus mongolica forest and Q. variabilis forest, etc. while the artificial forest was found to have 6 patterns including Castanea crenata, etc. In order to verify their age-class, this research measured a tree age by collecting 42 quadrats and 89 specimen tree cores on the basis of a detailed actual vegetation map; as a result, an artificial forest and oak trees with small diameters located at low-lying areas, was categorized as 2-grade age class(covering 29.8%), and other areas were judged to be available for land use as 3-grade age-class(covering 57.6%) while the areas judged to be 4-or-more grade age-class (covering 8.8%) was impossible for land use because they are located on a steep slope ridge line on a boundary. In case a proposed site for a small-scale development is judged as a natural forest with deciduous trees as mentioned above, it is necessary that a detailed stock map plan should be drawn up through a detailed investigation into actual vegetation and analysis of plant gathering structure & specimen trees. A detailed stock map plan includes the data that makes it possible to comprehensively judge natural property, scarcity, and diversity of vegetation; thus, it is considered that a detailed stock map plan will be useful in judging the development propriety of a small-scale site.

• 제어로봇시스템학회:학술대회논문집
• /
• 2005.06a
• /
• pp.1304-1308
• /
• 2005

This paper presents the case that the existing analog control systems were retrofitted with digital DCS(Distributed Control System)s for boiler unit in thermal power plant. Replacement of existing worn-out or obsolete analog control systems has been considered. Replacement of existing analog control systems with another analog control systems has lots of difficulties in maintaining the systems due to being out of stock. Due to those difficulties, existing analog control systems have been retrofitted with digital DCSs in many industrial sites. KEPRI(Korea Electric Power Research Institute) accomplished the project that retrofitted analog control systems with the developed DCSs for boiler unit in middle-scale coal-fired thermal power plant. The benefits of an upgrade to digital control include a increase of reliability due to system redundancy, ease of modifying the control logic and the parameters of function block, ease of maintenance due to available spare parts, improvement of information display, ease of modifying MMI(Man Machine Interface) displays, a increase in system availability, and improvement of control performance. This paper describes how to use the parameters of existing analog controllers, the implementation of digital PID controller, control system configuration for boiler unit in thermal power plant, some boiler control loops, control result during commissioning, and the comparison of boiler characteristic test data after retrofitting with the existing test data.