• Asian-Australasian Journal of Animal Sciences
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• 제25권12호
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• pp.1674-1680
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• 2012

The purpose of this study was to detect significant SNPs for blood components that were related to immunity using high single nucleotide polymorphism (SNP) density panels in a Korean native pig (KNP)${\times}$Yorkshire (YK) cross population. A reciprocal design of KNP${\times}$YK produced 249 $F_2$ individuals that were genotyped for a total of 46,865 available SNPs in the Illumina porcine 60K beadchip. To perform whole genome association analysis (WGA), phenotypes were regressed on each SNP under a simple linear regression model after adjustment for sex and slaughter age. To set up a significance threshold, 0.1% point-wise p value from F distribution was used for each SNP test. Among the significant SNPs for a trait, the best set of SNP markers were determined using a stepwise regression procedure with the rates of inclusion and exclusion of each SNP out of the model at 0.001 level. A total of 54 SNPs were detected; 10, 6, 4, 4, 5, 4, 5, 10, and 6 SNPs for neutrophil, lymphocyte, monocyte, eosinophil, basophil, atypical lymph, immuno-globulin, insulin, and insulin-like growth factor-I, respectively. Each set of significant SNPs per trait explained 24 to 42% of phenotypic variance. Several pleiotropic SNPs were detected on SSCs 4, 13, 14 and 15.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.77-82
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• 2004

Quantifying rates of microbial processes under subsurface conditions is difficult, and is most commonly approximated by laboratory studies using aquifer materials. In this study a single-well, 'push-pull' test method is adapted for the in situ determination of denitrification rates in groundwater aquifers. The rates of stepwise reduction of nitrate to nitrite, nitrous oxide, and molecular nitrogen were determined by performing a series of push-pull tests at an experimental well field of Korea University. A single Transport Test, one Biostimulation Test, and four Activity Tests were conducted for this study. Transport tests are conducted to evaluate the mobility of solutes used in subsequent tests. These included bromide (a conservative tracer), fumarate (a carbon and/or source), and nitrate (an electron acceptor). At this site, extraction phase breakthrough curves for all solutes were similar, indicating apparent conservative transport of the solutes prior to biostimulation. Biostimulation tests were conducted to stimulate the activity of indigenous heterotrophic denitrifyinc microorganisms. Biostimulation was detected by the simultaneous production of carbon dioxide and nitrite after each injection. Activity tests were conducted to quantify rates of nitrate, nitrite, and nitrous oxide reduction. Estimated zero-order degradation rates decreased in the order nitrate '||'&'||'gt; nitrite '||'&'||'gt; nitrous oxide. The series of push-pull tests developed and field tested in this study should prove useful for conducting rapid, low-cost feasibi1ity assessments for in situ denitrification in nitrate-contaminated aquifers.

• Asian-Australasian Journal of Animal Sciences
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• 제28권7호
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• pp.936-942
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• 2015

The purpose of this study was to characterize genetic architecture of behavior patterns in Sapsaree dogs. The breed population (n=8,256) has been constructed since 1990 over 12 generations and managed at the Sapsaree Breeding Research Institute, Gyeongsan, Korea. Seven behavioral traits were investigated for 882 individuals. The traits were classified as a quantitative or a categorical group, and heritabilities ($h^2$) and variance components were estimated under the Animal model using ASREML 2.0 software program. In general, the $h^2$ estimates of the traits ranged between 0.00 and 0.16. Strong genetic ($r_G$) and phenotypic ($r_P$) correlations were observed between nerve stability, affability and adaptability, i.e. 0.9 to 0.94 and 0.46 to 0.68, respectively. To detect significant single nucleotide polymorphism (SNP) for the behavioral traits, a total of 134 and 60 samples were genotyped using the Illumina 22K CanineSNP20 and 170K CanineHD bead chips, respectively. Two datasets comprising 60 (Sap60) and 183 (Sap183) samples were analyzed, respectively, of which the latter was based on the SNPs that were embedded on both the 22K and 170K chips. To perform genome-wide association analysis, each SNP was considered with the residuals of each phenotype that were adjusted for sex and year of birth as fixed effects. A least squares based single marker regression analysis was followed by a stepwise regression procedure for the significant SNPs (p<0.01), to determine a best set of SNPs for each trait. A total of 41 SNPs were detected with the Sap183 samples for the behavior traits. The significant SNPs need to be verified using other samples, so as to be utilized to improve behavior traits via marker-assisted selection in the Sapsaree population.

• 한국산학기술학회논문지
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• 제10권1호
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• pp.194-199
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• 2009

우수한 항산화제로 알려진 astaxanthin을 함유한 H. pluvialis 균체로부터 작용기작이 상이한 exe형과 endo형의 단백질 분해 효소와 복합 다당류 분해 효소를 이용하여 식품용 haematococcus추출물을 효율적으로 제조할 수 있는 방법을 조사하였다. 상업용 단백질 분해 효소로는 Alcalase (endo형)와 Flavourzyme (exe형)을 복합 다당류 분해효소로는 Viscozyme을 사용하였다. 단백질 분해 효소는 endo형과 exe형을 병용하는 것이 추출물의 astaxanthin 함량을 증가시켰다. Viscozyme과 함께 2종류의 단백질 분해 효소를 사용하는 경우에는 Alcalase와 Flavourzyme을 병용하여 1차로 처리한 후 Viscozyme을 2차로 사용하는 2단계 가수분해 방법이 적절하였다. 이 조건에서 astaxanthin 함량은 효소를 사용하지 않은 대조구에 비하여 320% ($529{\mu}g/g{\rightarrow}2,256{\mu}g/g$) 이상 향상되었다. 또한 DPPH법으로 조사한 항산화 활성은 astaxanthin 함량에 비례하여 증가하였으며, 1차로 Alcalase와 Flavourzyme을 병용하여 처리한 후 2차로 Viscozyme을 사용하는 조건에서 가장 우수하였다.

• Ocean and Polar Research
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• 제24권3호
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• pp.179-187
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• 2002

The Ayu Trough, located in the southern end of the Philippine Sea, represents a divergent boundary between the Philippine Sea and the Caroline Plates. A detailed geophysical survey was carried out in the Ayu Trough by R/V Onnuri. Topographically, the Ayu Trough resembles an slow spreading ridge. The trough can be divided into three sections: the south $(0^{\circ}-1^{\circ}30'N),\;middle\;(1^{\circ}30'-4^{\circ}N)$, and north $(4^{\circ}-6^{\circ}30'N)$. The seafloor in the middle section is characterized by features asymmetric with respect to the axis. These features were probably produced by NW-SE and NNW-SSE extensions and seem to support the argument that the opening of the Ayu Trough occurred in an oblique fashion. Farther south, a long transform fault but with a short offset defines the boundary between middle and southern sections. The axial depth increases a stepwise to the south of $1^{\circ}30'N$. A clear difference can be seen between the southern and middle sections with the latter exhibiting much higher mantle Bouguer anomaly values in the axial region. The anomaly indicates that the axial crust perhaps experienced a much higher degree of extension in the middle than in the southern section. The analyses of magnetic field data reveal that the region beyond 100km exhibits considerable variations, whereas the magnetic anomalies within 100km from the trough axis are very much subdued. This observation suggests that the opening of the Ayu Trough involved an initial stage of rifting of existing volcanic arcs, followed by production of new seafloor.

• Journal of Crop Science and Biotechnology
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• 제10권1호
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• pp.33-38
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• 2007

Nitrogen management at the panicle initiation stage(PI) should be fine-tuned for securing a concurrent high yield and high quality rice production. For calibration and testing of the recommendation models of N topdressing rates at PI for target grain yield and protein content of rice, three split-split-plot design experiments including five rice cultivars and various N rates were conducted at the experimental farm of Seoul National University, Korea from 2003 to 2005. Data from the first two years of experiments were used to calibrate models to predict grain yield and milled-rice protein content using shoot fresh weight(FW), chlorophyll meter value(SPAD), and the N topdressing rate(Npi) at PI by stepwise multiple regression. The calibrated models explained 85 and 87% of the variation in grain yield and protein content, respectively. The calibrated models were used to recommend Npi for the target protein content of 6.8%, with FW and SPAD measured for each plot in 2005. The recommended N rate treatment was characterized by an average protein content of 6.74%(similar to the target protein content), reduced the coefficient of variation in protein content to 2.5%(compared to 4.6% of the fixed rate treatment), and increased grain yield. In the recommended N rate treatments for the target protein content of 6.8%, grain yield was highly dependent on FW and SPAD at PI. In conclusion, the models for N topdressing rate recommendation at PI were successful under present experimental conditions. However, additional testing under more variable environmental conditions should be performed before universal application of such models.

• Journal of the Korean Data and Information Science Society
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• 제27권4호
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• pp.969-977
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• 2016

야외에서 재배되는 주요 채소류의 생산에 대한 기상변화의 영향력이 점차 커지고 있다. 기상변화로 인한 농작물 생산량의 변화는 공급과 수요의 불안정과 물가안정의 불안요소로 작용하고 있다. 본 논문에서는 패널회귀모형을 이용하여 기상상태에 따른 마늘의 생산량을 추정하였다. 2006년부터 2015년까지의 마늘 주산지 15곳의 10a당 마늘 생산량과 해당 지역의 기상자료를 사용하였다. 7가지 기상요인 (평균기온, 평균최저기온, 평균최고기온, 누적강수량, 누적일조시간, 평균상대습도, 평균지면온도)의 월별 (1월-12월)자료인 총 84개 기상변수중 다중회귀분석 단계선택방법을 통하여 7가지 기상변수를 선택하여 패널회귀모형에 사용하였다. 고정효과 모형과 확률효과 모형을 구분하는 하우스만 검정을 통하여 확률효과 모형으로 분석한 결과 평균최고기온 (1월), 누적강수량 (3월, 10월), 누적일조시간 (4월, 10월)등이 마늘 생산량 추정에 유의한 변수로 나타났다. 또한 연도별로 추정된 생산량 추정값의 추이가 실제 생산량과 동일한 추세를 보이고 있어 제안된 패널 회귀 모형이 잘 적합됨을 확인할 수 있다.

• 말소리와 음성과학
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• 제3권1호
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• pp.145-151
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• 2011

In view of the hypothesis that the effects of Parkinson's disease on voice production can be detected before pharmacological intervention, the prosodic features of patients with idiopathic Parkinson's disease (IPD) and a healthy aging group were diagnostically analyzed with the long term object of establishing, for clinical purposes, early disease-progression biomarkers. Twenty patients (male 8; female 12) with IPD (prior to pharmacological intervention) and a healthy control group of 22 (male 10; female 12) were selected. Ten sentences were recorded with a head-worn microphone. One sentence was chosen for the analysis of this paper. Relevant parameters, i.e. 3-dimensional model (F0, intensity, duration) and pitch and intensity related slopes (maxEnergy, maxF0, meanAbS, semiT, meanEnergy, meanF0), were analyzed by two-group discriminant analysis. The stepwise estimation method of discriminant analysis was performed by gender. The discriminant functions predicted 83.9% of the male test data correctly while the prediction rate was 93.1% for the female group. The results showed that meanF0_slope and semiT_slope were more important parameters than the others for the male group. For the female group, the meanEnergy_slope and maxEnergy_slope were the important ones. These findings indicate that significant parameters are different for the male and female group. Gender lifestyle may be responsible for this difference. Dysprosodic features of IPD show not simultaneously but progressively in terms of F0, intensity and duration.

• Preventive Nutrition and Food Science
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• 제10권4호
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• pp.357-363
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• 2005

The purpose of this study was to assess current food-handling practices of employees in school food service settings, as well as their knowledge levels, and identify relationships between knowledge, practices, and influencing variables. The survey was conducted for dietitians and employees in the school foodservice industry in Gyeongsangbuk-do province. A total of 270 and 570 questionnaires for dietitians and employees, respectively, were distributed by mail. Response rates were $62\%$ (N=171) and $66\%$ (N=376) from dietitians and employees, respectively. Data was analyzed using SPSS Windows (version 10.0). Descriptive statistics were used to summarize data. Pearson correlations were applied to test for relationships between knowledge and practice of HACCP principles. Stepwise regression analysis was performed to examine the influence of knowledge, current education guidelines, demographic information (working experience, academic background, and certification for food and cooking), and school characteristics (food production system, service style, and number of meals). School foodservice employees were found to have a significant amount of food safety knowledge ($67.5\pm1.8$ out of 100 possible points). Proper food handling practices were not always being followed in many schools. The relationship between their knowledge, current HACCP education training, and food handling practices was not significant. These results suggested the present situation of HACCP trainings performed by dietitians were inadequate for many school foodservice operations. The number of meals in school was an independent predictor of the employees' food-handling practices. These results suggest that an effective education program should integrate endeavors that take account of social and environmental influences on food safety to support the improvement of food-handling practices and the implementation of a HACCP program. Furthermore, dietitians should continue to provide consulting, training, and technical assistance to schools on HACCP implementation.

• 한국식품과학회지
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• 제32권6호
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• pp.1326-1330
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• 2000

김치발효중 기체조성변화가 숙성정도를 감지할 수 있는지 알아보기 위하여 배추김치를 플라스틱 용기에 일정량 충진하여 밀봉한 후 저장하면서 포장내 기체조성 변화를 조사한 결과, 김치발효중 포장내 기체조성은 $CO_2$ 발생으로 인하여 계속적으로 변화하였다. 김치 발효온도와는 무관하게 $O_2$ 농도는 지수적으로 감소하고 $CO_2$ 농도는 매우 특징적인 2단계 증가양상을 나타내었다. $CO_2$ 농도는 2차 단계에서 다시 증가함에 따라 김치의 pH는 비례적으로 감소하였다. 저장 온도와 계절별 원료 배추에 따라 김치 포장내 $CO_2$ 농도변화 속도가 다르며 최종 농도에 있어서도 상당한 차이를 나타내었다. 고온에서 발효된 김치일수록 포장내 $CO_2$ 농도가 더 높은 경향을 보였고 여름배추 보다 겨울이나 봄 배추로 제조한 김치에서 포장내 $CO_2$ 농도 및 발생속도가 더 높게 나타났다. 이러한 연구결과를 바탕으로 포장내 기체조성 변화를 이용한 김치의 숙성도 감지 가능성을 확인할 수 있었다.