• 약학회지
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• 제54권5호
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• pp.398-402
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• 2010

[ ${\alpha}$ ]Glucosidase inhibitor is a target in the treatment of type II diabetes through the mainly inhibition of glucose levels after meals. In this study, we purified steppogenin and oxyresveratrol from the stem of Morus alba L. and examined their inhibitory activity against yeast ${\alpha}$-glucosidase. Steppogenin and oxyresveratrol were inhibited yeast ${\alpha}$-glucosidase in a dose dependent manner. The $IC_{50}$ activities (50% inhibition) were 34.4 and 9.3 ${\mu}M$, respectively. The kinetic inhibition of steppogenin showed noncompetitive inhibition ($K_m:1.1{\times}10^{-3}M$; $K_i:1{\times}10^{-5}M$), meanwhile oxyresveratrol showed competitive inhibition ($K_m:4.3{\times}10^{-3}M$; $K_i:3.4{\times}10^{-6}M$) against yeast ${\alpha}$-glucosidase. These results indicate that steppogenin and oxyresveratrol are noncompetitive and competitive inhibitors, respectively, against yeast ${\alpha}$-glucosidase.

• 생약학회지
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• 제51권2호
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• pp.100-106
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• 2020

The extract of Cudrania tricuspidata is used in ethnomedicine throughout Eastern Asia in China, Korea and Japan. In Korean traditional medicine, Cudrania tricuspidata has been used to treat eczema, mumps, tuberculosis, contusions, insomnia and acute arthritis. In addition, it has been reported that root extract of Cudrania tricuspidata has anti-platelet effects. Therefore, we investigated which compound in Cudrania tricuspidata has inhibitory effect on platelet aggregation. In this study, we tried to explain the inhibitory mechanism of steppogenin from Cudrania tricuspidata on human platelet aggregation. Collagen-induced human platelet aggregation and [Ca²⁺]_i mobilization were dose-dependently inhibited by steppogenin and we determined the inhibition by steppogenin is due to the down regulation of extracellular-signal-regulated kinase(ERK) and inositol-1,4,5-triphosphate receptor type I(IP₃RI) phosphorylation. In addition, steppogenin inhibited collagen-induced fibronectin adhesion to αIIb/β3 and thromboxane A₂ generation. Thus, in the present study, steppogenin showed an inhibitory effect on human platelet aggregation, suggesting its potential use for preventing platelet-induced cardiovascular disease.

• Applied Biological Chemistry
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• 제37권2호
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• pp.105-109
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• 1994

꾸지 뽕나무의 줄기껍질로부터 P388 tumor cell line에 대한 세포 성장 저해활성과 흰쥐 간 microsome 분획의 과산화 지질을 사용하여 $Fe^{++}/\;ascorbate$법으로 측정한 결과 세포 성장 저해활성과 항산화활성을 갖는 5개의 flavonoid계 화합물을 분리하였다. 분리된 화합물의 구조분석을 실시한결과 각종 spectral data와 보고된 문헌에 의하여 taxifolin, orobol, eriodictyol, dihydrokaempferol, steppogenin으로 각각 동정되었다. 이 화합물들의 항산화활성은 $IC_{50}$이 각각 6, 3, 3, >50, $10\;{\mu}g/ml$이었고 taxifolin을 제외한 P388 cell line에 대한 세포 성장저해는 각각 $IC_{50}$이 0.18, 3.3, 15, $6.2\;{\mu}g/ml$이었다. 한편 Escherichia coli BE 1186, Salmonella thyphimurium SL 1102, Staphylococcus aureus IFO 12732, Staphylococcus aureus R 209, Candida albicans에 대해서는 항균활성을 나타내지 않았다.