• JSTS:Journal of Semiconductor Technology and Science
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• 제15권1호
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• pp.22-28
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• 2015

This paper proposes column-parallel three step Single Slope Analog-to-Digital Converter (SS-ADC) for high frame rate VGA CMOS Image Sensors (CISs). The proposed three step SS-ADC improves the sampling rate while maintaining the architecture of the conventional SS-ADC for high frame rate CIS. The sampling rate of the three-step ADC is increased by a factor of 39 compared with the conventional SS-ADC. The proposed three-step SS-ADC has a 12-bit resolution and 200 kS/s at 25 MHz clock frequency. The VGA CIS using three step SS-ADC has the maximum frame rate of 200 frames/s. The total power consumption is 76 mW with 3.3 V supply voltage without ramp generator buffer. A prototype chip was fabricated in a $0.13{\mu}m$ CMOS process.

• 생명과학회지
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• 제31권4호
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• pp.385-388
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• 2021

벼 육종을 위한 약 배양은 기존 육종 방법을 사용하여 새로운 품종을 개발하는데 최소 6세대에 필요한 시간을 크게 줄여 동형접합자를 신속하게 생산하는 방법이다. 이 약 배양기술은 방법론적 관점에서 더 많은 녹색 식물을 얻을 기회를 제공하며, 약 배양에서 시간과 노력을 절약하는 배양의 효율성을 높이기 때문에 중요하다. 본 연구에서는 배양액과 배양 방법이 다른 1단계와 2 단계 배양의 캘러스 유도율과 녹색 식물 재분화율로 비교하였다. 1단계 배양은 하나의 배지에서 캘러스 유도 및 식물 재생을 허용하는 반면, 2 단계 배양은 두 개의 다른 배지에서 유도 및 식물 재분화가 필요하다. 이 연구에서는 1 단계 및 2 단계 배양으로 벼 꽃밥의 캘러스 유도와 식물 재생률을 비교했다. 캘러스 형성률은 1 단계 배양의 경우 13.0 %, 2 단계 배양의 경우 8.6%로 2 단계 배양보다 1 단계 배양에서 4.4% 더 높았다. 식물 재분화율은 1 단계 배양에서 1.0%, 2 단계 배양에서 3.0 %로 1 단계 배양보다 2단계 배양에서 식물체 재분화율이 3배 더 높았다. 이것은 2 단계 배양이 반수체 생산을 위한 1 단계 배양보다 더 효율적임을 시사한다.

• Journal of Mechanical Science and Technology
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• 제14권5호
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• pp.547-552
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• 2000

The objective of this paper is to present a modified fractional step method of keeping a constant mass flow rate in spatially periodic flows, because original fractional step methods do not precisely keep the mass flow rate constant in time. In the modified method, the mean and fluctuating pseudo-pressure gradients are separately obtained at each time step. This method is successfully applied to channel and pipe flows and shown to be suitable for maintaining a constant mass flow rate in time.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1871-1880
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• 2016

Bio-oxidation is an effective technology for treatment of refractory gold concentrates. However, the unsatisfactory oxidation rate and long residence time, which cause a lower cyanide leaching rate and gold recovery, are key factors that restrict the application of traditional bio-oxidation technology. In this study, the oxidation rate of refractory gold concentrates and the adaption of microorganisms were analyzed to evaluate a newly developed two-step pretreatment process, which includes a high temperature chemical oxidation step and a subsequent bio-oxidation step. The oxidation rate and recovery rate of gold were improved significantly after the two-step process. The results showed that the highest oxidation rate of sulfide sulfur could reach to 99.01 % with an extreme thermophile microbial community when the pulp density was 5%. Accordingly, the recovery rate of gold was elevated to 92.51%. Meanwhile, the results revealed that moderate thermophiles performed better than acidophilic mesophiles and extreme thermophiles, whose oxidation rates declined drastically when the pulp density was increased to 10% and 15%. The oxidation rates of sulfide sulfur with moderate thermophiles were 93.94% and 65.73% when the pulp density was increased to 10% and 15%, respectively. All these results indicated that the two-step pretreatment increased the oxidation rate of refractory gold concentrates and is a potential technology to pretreat the refractory sample. Meanwhile, owing to the sensitivity of the microbial community under different pulp density levels, the optimization of microbial community in bio-oxidation is necessary in industry.

• 한국음향학회지
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• 제28권8호
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• pp.815-821
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• 2009

소음이 존재하고, 신호의 전달 시스템이 가변적인 환경에서 신호처리는 일반적으로 적응 알고리즘에 의해 이뤄진다. 다양한 적응 알고리즘들 중에서 LMS 알고리즘은 연산량이 적고, 구현이 쉬우며, 성능이 훌륭해 가장 널리 쓰이는 알고리즘이 되었다. LMS 알고리즘의 성능에 영향을 미치는 가장 중요한 요소 중 하나가 Step Size이다. 일반적으로, Step Size가 크면, 알고리즘 수렴 속도는 빨라지지만, 수렴 오차는 커지게 되고, Step Size가 작으면 수렴 오차는 작아지지만, 수렴 속도는 느려진다. Step Size의 이러한 특성을 상호 보완하고자 많은 방법들이 제안되어 오고 있다. 본 논문에서는 오차 제곱 변화 곡선의 기울기로부터 현재 상태에 대한 카테고리를 분류하여, Step Size를 매 단계마다 적절하게 가변시킴으로써, 결과적으로 수렴 속도와 정확도, 연산량을 향상시킨 새로운 개념의 Categorized 가변 스텝 사이즈 LMS 알고리즘을 제시하고, 그 성능은 실험을 통하여 수렴 속도와 Excessive Mean Square Error (EMSE), 연산량의 관점에서 향상되었음을 검증하였다.

• Clinical and Experimental Reproductive Medicine
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• 제27권2호
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• pp.191-200
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• 2000

Objective: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. Materials and Methods: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate of the vitrified and ultra-rapid frozen mouse mature oocytes. Results: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61 %, 54% respectively. There were no significant differences among treatments(p>0.05). The morphological normality and fertilization rate of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). Conclusion: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.

• Journal of Photoscience
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• 제9권2호
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• pp.130-133
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• 2002

The last step in the photocycle of photoactive yellow protein (PYP) is a spontaneous recovery of the dark state from the active state in which the p-coumaric acid chromophore is thermally isomerized, concomitantly with the deprotona- tion of the chtomophore and the refolding of the protein moicty. For the purpose of understanding the mechanism of the thermal back-isomerization, we have investigated the rate-determining step by analyzing mutant PYPs of Met100, which was previously shown to play a major role in facilitating the reaction (1). The mutation to Lys, Leu, Ala, or Glu decelerated the dark state recovery by 1 to 3 three orders of magnitude. By evaluating temperature-dependence and pH-dependence of the kinetics of the dark state recovery, it was found that the retardation by mutations resulted from elevation of the activation enthalpy ( H$\^$┿/) and that the pKa of the chromophore, which was affected by the mutation, is in a linier correlation with the amplitude of the rate constants. It was, therefore, deduced from the correlation that the free energy for crossing the activated state in the dark recovery process is proportional to the free energy for the deprotonation of the chromophore, identifying the rate-determining step as the deprotonation of the chromophore. (1) Devanathan, S. Genick, U. K. Canestrelli, I. L. Meyer, T. E. Cusanovich, M. A. Getzoff, E. D. Tollin, G., Biochemistry 1998, 37, 11563 - 11568

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제4권2호
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• pp.57-64
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• 2015

피크 임계값을 사용하는 걸음 검출 알고리즘에서 충분한 정확도로 걸음을 검출하기 위해서는 3축 가속도 센서가 20Hz 이상의 주파수로 샘플링을 수행하여야 한다. 그러나 $I^2C$나 SPI를 통하여 데이터를 전송받는 상용의 통합 MPU와 연결되는 디지털 센서 장치들의 샘플링 주파수는 아날로그 방식의 샘플링 회로들에 비하여 매우 낮은 경향이 있다. 센서의 샘플링 주파수가 낮게 되면 충분한 데이터를 확보할 수 없기 때문에 측정 결과의 정확도가 떨어지게 된다. 본 연구에서는 피크 임계값 방식의 피크 검출 알고리즘에서 데이터가 20Hz 이하의 낮은 주파수로 샘플링될 경우에 샘플링 주파수와 피크 임계값 사이에 함수관계가 있음을 발견하였으며, 실험을 통하여 임계 함수를 도출하였다. 고정 임계값 대신에 샘플링 주파수에 따른 임계 함수를 적용하고, 테스트 프로토콜에 의하여 실험을 수행한 결과, 각 걸음 유형에 대하여 평균적으로 1.2% 미만의 걸음 검출 오차율을 얻을 수 있었다. 그러므로 걸음 검출 알고리즘이 걸음 모드에 따라서 적절히 결정된 임계 함수로부터 샘플링 주파수에 적합한 임계값을 사용하여 걸음을 검출한다면, 걸음 검출 및 걸음수 측정의 정확도는 매우 높아질 수 있다. 이러한 결과는 걸음수 측정 장치에만 적용되는 것이 아니라, 샘플링 주파수가 낮게 설계될 수밖에 없는 소형, 저가의 유비쿼터스 기기에도 적용해 정확도를 효과적으로 향상시킬 수 있다.

• Bulletin of the Korean Chemical Society
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• 제35권11호
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• pp.3313-3318
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• 2014

The reaction mechanism of Lead ions catalyzing complexation reactions between TIPP and metal ions was investigated by researching the kinetics of the formation of metalloporphyrins by UV/Vis-spectra, and verified by exploring the formation of metalloporphyrins catalyzed by acetic acid. Kinetics studies suggested that the fluctuations of reaction rate indicated the formation of metalloporphyrin was step-wise, including the pre-equilibrium step (the coordination of the pyrrolenine nitrogens to $Mn^+$) and the rate-controlling step (the deprotonation of the pyrrole proton). In the pre-equalization step, a sitting-atop (SAT) structure formed first with the complexation between larger radius of $Pb^{2+}$ and TIPP, changed the activation, then $Pb^{2+}$ left with the smaller radius of metal ions attacking from the back of the porphyrin ring center. In the rate-controlling step, two pyrrole protons dissociated to restore a stable structure. This was verified by adding acetic acid at different reaction times.

• Clinical and Experimental Reproductive Medicine
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• 제19권2호
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• pp.143-152
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• 1992

The present experiments were focussed to modify a short slow-cooling protocol used for freezing of early stage embryo(Testart et al., 1986) and also to apply the modified method for the cryopreservation of hamster oocytes with Zona or without. The protocol was modified by changing the 4-step equilibration into 1-step and the 1-step thawing into 2-step. The oocytes were added in 1.5M PROH and 0.1M Sucrose, seeded at $-7^{\circ}C$, slow cooled($0.3^{\circ}C$/min) to $-30^{\circ}C$ before plunging to $-196^{\circ}C$. The oocytes were thawed at $23-25^{\circ}C$ air(20sec/150sec) and/or 33-35 water(10sec). The survival of the frozen-thawed oocytes was determined by morphologic criteria and their fertilizing ability was also estimated by Sperm Penetration Assay(SPA) system(Chang et al, 1990) using fertile men semen sample. One-step equilibration showed slightly higher survival rate(83.9% vs. 71.0%) and fertilization rate(83.9% vs. 71.0%) compared with four-step(p>0.05). And two-step thawing(air & water exposing) of oocytes frozen after 1-step equilibration showed significantly higher survival rate(96.3%) than one-step thawing at air(85.2%) or water(65.0%) only(p<0.05). Therefore, by the modified method(l-step equilibration & 2-step thawing), Zona-intact(ZI) and Zona-free(ZF) oocytes were frozen and thawed. ZI-oocytes showed significantly higher survival rate(95.4%, 308/323 vs. 67.6%, 240/355) than ZF-oocytes(P<0.01). But the survival of ZF-oocytes was as high as ZI-oocytes in fourteen of twenty-four replicates. ZI-oocytes was also significantly higher fertilization rate($92.4{\pm}8.9%$ vs. $63.7{\pm}18.5%$) and higher mean number of penetrated sperm($6.2{\pm}4.2$ vs. $3.9{\\pm}3.3$) than ZF-oocytes, but not higher than control(fresh oocytes;$99.3{\pm}2.4%$, $8.4{\pm}4.2$)(P<0.001). Conclusively, this modified method will contribute to freeze effectively the hamster oocytes for simplifing of the logical consideration of performing SPA and also to freeze the human and other animal oocytes.