• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.44-48
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• 1998

Two determinate soybean cultivars, 'Baegunkong' and 'Namhaekong', were planted on 8 Jone, 23 June, and 8 July 1996 at Cheju at planting densities of 33, 43, 53, 63 plants per $m^2$ to determine the optimum planting density of double crop soybean for recently recommended cultivars in Cheju area at various planting dates. The plant height, and the diameter and node number of main stems decreased as planting was delayed. The plant height increased but the stem diameter and node number decreased with increasing planting density. Pod number per plant was greater for Namhaekong than for Baegunkong and was not affected by planting date. Pod number per plant decreased but pod numbers per $\textrm{m}^2$ increased with increasing planting density. The number of seeds per pod was greater at the two later plantings and fewer at the highest planting density. 100-seed weight decreased with delaying planting. The seed weight was lighter at the highest planting density for Baegunkong but there was no difference for Namhaekong among the planting densities. The seed yield of Baegunkong was greater for 23 June and 8 July plantings (2,280 and 2,420 kg/ha) than for 8 June planting (1,450 kg/ha) while that of Namhaekong was greatest at 23 June planting (2,690 kg/ha) compared with 8 June and 8 July plantings (1,890 and 2,080 kg/ha). Across the planting dates and cultivars, seed yield increased from 1,860 to 2,290kg/ha as the planting density increased from 33 to 53 plants/$\textrm{m}^2$ and then leveled off with a further increase in planting density.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.548-554
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• 2016

Optimal range of soil physical quality to enhance crop productivity or to improve environmental health is still in dispute for the upland soil. We hypothesized that the optimal range might be established by comparing soil physical parameters and their interactions inhibiting crop growth. The parameter identifying optimal range covered favorable conditions of aeration, permeability and root extension. To establish soil physical standard two experiments were conducted as follows; 1) investigating interactions of bulk density and aeration porosity in the laboratory test and 2) determining effects of soil compaction and deep & conventional tillage on physical properties and crop growth in the field test. The crops were Perilla frutescens, Zea mays L., Solanum tuberosum L. and Secale cereael. The saturated hydraulic conductivity, bulk density from the root depth, root growth and stem length were obtained. Higher bulk density showed lower aeration porosity and hydraulic conductivity, and finer texture had lower threshold bulk density at 10% aeration bulk density. Reduced crop growth by subsoil compaction was higher in silt clay loam compared to other textures. Loam soil had better physical improvement in deep rotary tillage plot. Combined with results of the present studies, the soil physical quality was possibly assessed by bulk density index. Threshold subsoil bulk density as the upper value were $1.55Mg\;m^{-3}$ in sandy loam, $1.50Mg\;m^{-3}$ in loam and $1.45Mg\;m^{-3}$ in silty clay loam for optimal soil physical quality in upland.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1017-1021
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• 2012

A field experiment was conducted to evaluate effect of water soluble silicate fertilizer (WSS) application on rice plants with respect to comparing with powdery slag-originated silicate fertilizer (PSS) and granular one (GSS). The 30-day seedlings were transplanted on May 10, 2012. The plot size was $25m^2$, and the planting density was 15 hills $m^{-2}$. The standard application level was $2kg\;ha^{-1}$ for WSS, $200kg\;ha^{-1}$ for GSS, $200kg\;ha^{-1}$ for GSS. The application rates were 50 %, 100 %, and 200 % of the standard levels. The soil and plant samples were taken after harvest on September 10. Strength weight of the stem was measured on the center of the 5-cm of the fourth internode. Nutrient contents and yield of grains and were evaluated. The strength weight of the stem was positively correlated with the silicate content of the stem with the highly significant $R^2$ of 0.601. The strength of the stem was satisfactorily enforced by application of 50 % WSS and GSS, and 100 % PSS. Application of 50 % or 100 % of WSS showed little difference in rice yield in comparison with application of 100 % of PSS or GSS. Therefore, application of $20kg\;ha^{-1}$ of WSS would be recommendable for rice cultivation which enforced stem strength, and increased yield of rice.

• Korean Chemical Engineering Research
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• v.51 no.6
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• pp.727-732
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• 2013

In this report, we present an inverse opal scaffold that can enhance the chondrogenic differentiation of human adipose-derived stem cells (hADSCs) without drug, gene, or cytokine supplement. Inverse opal scaffolds based on poly(D,L-lactide-co-glycolide) were formed with uniform $200{\mu}m$ pores. Due to uniform pore sizes and well-controlled interconnectivity of inverse opal scaffold, hADSCs were allowed to distribute homogeneously throughout the scaffolds. As a result, high cell density culture with scaffold was possible. Since the hADSCs cultured in inverse opal scaffolds were subjected to limited supplies of oxygen and nutrients, these cells were naturally preconditioned to a hypoxic environment that stimulated the up-regulation of hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$). As a result, apoptotic activity of hADSCs until 3 weeks after initial cell seeding was significantly reduced and chondrogenic differentiation related molecular signal cascades were up regulated (transforming growth factor-beta, phosphorylated AKT, and phosphorylated p38 expression). In contrast, hADSCs cultured with small and non-uniform porous scaffolds showed significantly increased apoptotic activity with decreased chondrogenic differentiation. Taken together, inverse opal scaffold could potentially be used as an effective tool for improving chondrogenesis using stem cells.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.43-48
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• 2001

This study was investigated to find out the optimum planting densities of Scutellaria baicalensis GEORGE about growth characters, yield components and yield at 3 planting densities by direct sowing cultivated after barley Stem length was long In dense planting of 20$\times$10cm and short in spacious planting of 30$\times$10cm and 40$\times$10cm by direct sowing cultivated after barley. Stem diameter was thick in spacious planting of 30$\times$10cm and 40$\times$10cm and was thin in dense planting of 20$\times$cm by direct sewing cultivated after barley. Length and dry weight of root per plant were decreased in dense planting of 20$\times$10cm and were increased in spacious planting of 30$\times$10cm and 40$\times$10cm by direct sowing cultivated after barley. Yield of dry root was highest in optimum planting density(30$\times$10cm:33 plants/$m^2$) by direct sowing cultivated after barley. The correlation coefficient between number of planting plant and stem length showed highly positive correlation. These characters of stem diameter, number of branches, main root length and yield of dry root mentioned above showed negative correlations with planting plants.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.77-80
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• 2002

This study was investigated to find out the optimum planting densities of Scutellaria baicalensis George about growth characters, yield components and yield at 3 planting densities by direct sowing cultivated after barley. Stem length was long in dense planting of 20$\times$l0cm and short in spacious planting of 30$\times$l0cm and 40$\times$10cm by direct sowing cultivated after barley. Stem diameter was thick in spacious planting of 30$\times$10cm and 40$\times$10cm and was thin in dense planting of 20$\times$10cm by direct sowing cultivated after barley. Length and dry weight of root per plant were decreased in dense planting of 20$\times$ 10cm and were increased in spacious planting of 30$\times$10cm and 40$\times$10cm by direct sowing cultivated after barley. Yield of dry root was highest in optimum planting density(30$\times$10cm：33 plants/㎡) by direct sowing cultivated after barley. The correlation coefficient between number of planting plant and stem length showed highly positive correlation. These characters of stem diameter, number of branches, main root length and yield of dry root mentioned above showed negative correlations with planting plants.

• Journal of Korea Foresty Energy
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• v.22 no.1
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• pp.49-57
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• 2003

This study was carried out with Urich II method for estimating the aboveground biomass of 30 years old Chamaecypris obtuse plantation in Jinhae, Cyeongnam province, in order to solve problems of exhaustion of many time and manpower in field survey for estimating biomass of stands. The results were summarize as follows; Total aboveground biomass production of stands was 137.3ton/ha, which was 71.8% of stem, 15.4% of branch and 12.8% of leaf. Foliage biomass of stands was maximized on the 9.2~11.2m of total height. The ratio of biomass for each part of tree in Chamaecypris obtusa plantation was in order of, stem, branch and leaf in dominant and co-dominant tree, and was stem, leaf and branch in recessive tree. drying ratio by components were ranged 47.3~49.2% of stem, 48.8~52.9% of branch, 39.2~40.8 of leaf and 0.78%~0.89% of died branch. Specific gravity by sample tree was 0.49~0.53 range and there was not significantly different among sample tree.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.221-228
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• 2014

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of $4{\times}10^5$ pSSCs on mitotically in activated $2{\times}10^5$ STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.2
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• pp.61-65
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• 2011

Biomass is considered to be a major potential fuel and renewable resource for the future. In fact, there is high potential to produce the large amount of energy from biomass around the world. In this study, to obtain basic data for practical application of agricultural and forest by-products as fuel of heating system in agriculture, agricultural and forest biomass resources were surveyed, the pelletizer with capacity of $50\;kg{\cdot}h^{-1}$ was designed and manufactured and pellets were made by the pelletizer. High heating value, ash content, etc. of pellets made of agricultural and forest by-products were estimated. Straw of rice was the largest agricultural biomass in 2009 and the total amount of rice straw converted into energy of $299{\times}10^3$ TOE. And in 2009, amount of forest by-product converted into energy of $9,579{\times}10^3$ TOE. High heating values of pellets made of stem and seed of rape, stem of oat, rice straw and rice husk were 16,034, 16,026, 16,089, 15,650, $15,044\;kJ{\cdot}kg^{-1}$ respectively. High heating values of pellets made of agricultural by-products were average 83.6% compared to that of wood pellet. Average bulk density of pellets made of stem and seed of rape, stem of oat, rice straw and rice husk was $1,400\;kg{\cdot}m^{-3}$ ($1.4\;g{\cdot}cm^{-3}$). Ash contents of the pellets were 6.6, 7, 6.2, 5.5, 33% respectively. Rice husk pellet produced the largest ash content compared to other kinds of pellets.

• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.201-209
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• 2018

Canine mammary tumors are among the most frequently observed cutaneous tumors in female dogs. Cancer stem cells (CSCs), referred to as tumor-initiating cells, are thought to have properties similar to normal stem cells such as the ability to self-renewal and to differentiate into various cell types. Biological understanding of CSCs and the critical pathways involved in their maintenance are important in research and therapy for mammary tumors. We conducted the present study on sphere formation from REM134 cells by using methylcellulose to produce tumorspheres on a large scale and compared the specific markers of the spheres-formed and plating-cultured REM134 cells. The results revealed that the tumorspheres cultured in methylcellulose had higher seeding density and improved morphology compared to those produced in normal sphere formation medium. Expression levels of stemness markers and CSC-related markers were higher in tumorsphere-forming cells than in plating-cultured cells. Subsequently, we transplanted the tumorsphere-forming and plating-cultured cells into female nude mice to examine their tumorigenic potential. Tumor volume increased rapidly in mice transplanted with tumorsphere-derived cells compared to plating-cultured cells. We observed a novel sphere-forming condition for REM134 cells and showed that REM134 cell tumorspheres can exhibit improved CSC properties.