• 대한조선학회논문집
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• 제29권4호
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• pp.87-97
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• 1992

극지방의 천연자원의 수송에 필수적인 빙해항행선박은 금후 우리나라의 조선산업이 지향해야 할 기술집약형 선박중 하나로서 국제경쟁에 대비하여 독자적인 연구가 요구되는 분야이다. 본 연구는 빙해항행선박 기본설계의 중요한 인자인 선수부 형상과 평탄빙에서의 쇄빙능력 사이의 상관관계를 밝히는데 목적이 있다. 통상 평탄빙(level ice)에서 선박의 전진속도를 3-4 knots로 볼 때, 탄성으로 취급되는 얼음의 재료특성을 고려하여, 파괴시킬 수 있는 얼음의 최대두께와 파괴된 얼음의 특성길이를 수치적으로 추정하는데 주안점을 두고 있다. 본 연구에서는 빙해항행 선박이 평탄빙에서 연속쇄빙을 하고 있는 상황을 탄성지지기반 위에 놓인 유한 길이의 쐐기보에 작용한 충격하중의 문제로 가정하고 굽힘모멘트에 의해 어떤 위치에서 발생한 최대인장응력이 얼음의 굽힘파괴강도에 도달한다면 그 부분에서 파단이 일어날 가능성이 가장 높다고 판단한다.

• Journal of Periodontal and Implant Science
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• 제52권3호
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• pp.242-257
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• 2022

Purpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells. Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay. Implants with 3D-engineered tissue were implanted into the lower right central incisors after extraction from rabbits. Forty implants (Ti or zirconia) were subdivided according to the duration of implantation (healing period: 45 or 90 days). Each subgroup (20 implants) was subdivided into 4 groups (without cells, PDLSC sheets, BMMSC sheets, and co-culture cell sheets). All groups underwent histological testing involving haematoxylin and eosin staining and immunohistochemistry, stereoscopic analysis to measure the PDL width, and field emission scanning electron microscopy (FESEM). The natural lower central incisors were used as controls. Results: The BMMSCs co-cultured with PDLSCs generated a well-formed PDL tissue that exhibited positive periostin expression. Histological analysis showed that the implantation of coated (Ti and zirconia) dental implants without a cell sheet resulted in a well-osseointegrated implant at both healing intervals, which was confirmed with FESEM analysis and negative periostin expression. The mesenchymal tissue structured from PDLSCs only or co-cultured (BMMSCs and PDLSCs) could form a natural periodontal tissue with no significant difference between Ti and zirconia implants, consequently forming a biohybrid dental implant. Green fluorescence for periostin was clearly detected around the biohybrid implants after 45 and 90 days. FESEM showed the invasion of PDL-like fibres perpendicular to the cementum of the bio-hybrid implants. Conclusions: β-TCP-coated (Ti and zirconia) implants generated periodontal tissue and formed biohybrid implants when mesenchymal-tissue-layered cell sheets were isolated from PDLSCs alone or co-cultured BMMSCs and PDLSCs.

• International Journal of Stem Cells
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• 제16권1호
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• pp.36-43
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• 2023

Background and Objectives: Lymphoblastoid cell lines (LCLs) deposited from disease-affected individuals could be a valuable donor cell source for generating disease-specific induced pluripotent stem cells (iPSCs). However, generation of iPSCs from the LCLs is still challenging, as yet no effective gene delivery strategy has been developed. Methods and Results: Here, we reveal an effective gene delivery method specifically for LCLs. We found that LCLs appear to be refractory toward retroviral and lentiviral transduction. Consequently, lentiviral and retroviral transduction of OCT4, SOX2, KFL4 and c-MYC into LCLs does not elicit iPSC colony formation. Interestingly, however we found that transfection of oriP/EBNA-1-based episomal vectors by electroporation is an efficient gene delivery system into LCLs, enabling iPSC generation from LCLs. These iPSCs expressed pluripotency makers (OCT4, NANOG, SSEA4, SALL4) and could form embryoid bodies. Conclusions: Our data show that electroporation is an effective gene delivery method with which LCLs can be efficiently reprogrammed into iPSCs.

• 한국자원식물학회지
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• 제11권
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• pp.89-109
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• 1998

Screening of new antioxidants form oriental medicines resulted in the isolation of a new antioxidative compound and eight known compounds from the stem bark of Kalopanax pictus. On the basis of various spectrosopic studies, the structure of the new compound was determined to be 4-rhamnose-3,5-dimethoxybenzoic acid methly ester. Other known compounds were identified as ferulic acid, 4,5,6,-trihydroxyflavanone, 2', 4',4' -trihydroxychalcone, caffeic acid, coniferyl alcohol, syringin, 1,3-di-O-caffeoylquinic acid. These compounds showed lipid peroxidation inhibitory acitivity in rat liver microsomes and free radical scavenging acitivity.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.459-466
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• 1994

Primordial germ cells (PGCs) in aves are the progenitor cells for the gametes. These cells first appear in the epiblast (Eyal-Giladi et al.. 1981). Then translocate and concentrate to endoderm of germinal crescent area in the junction of the area opaca and area pellucida lateral to the primitive streak in stage 4 through 7. They separate from the endoderm, temporarily circulate via the blood vascular system, leave the blood vessels, and finally settle down in the gonadal anlagen at stage 20-24 where they rapidly proliferate to form germ cells. Recently, several attempts have been made to introduce foreign gene into the avian genome to form a transgenic chicken. The stem cells most readily available as vehicles for genetic manipulation of germline in avian species are the PGCs. PGCs have recently been manipulated genetically and used successfully as a vector for gene transfer.

• 한국조경학회지
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• 제21권2호
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• pp.8-16
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• 1993

The purpose of this paper is to analyze the linguistic origin of the species manes of landscape plants in order to help students understand the physical characteristics of the plants through the botanical names. This study includes the plants that are possible to use as landscape material as well as both the native and foreign plants that are already used. Name of Species of Scientific name in Landscape plant be derived from region or nations of habitat, botanist, plant explors, and their sponser, and plant form and number of leaves or flowers, character or ingredient of plants and shape of stem, tree crown form. In this paper, about 300 species(contains varieties, formas) are interpreted into etymological meanings.

• 한국환경성돌연변이발암원학회지
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• 제23권2호
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• pp.56-62
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• 2003

The liver progenitor cells could form a potential target cell population fore both tumor-initiating and -promoting chemicals. Induction of drug-metabolizing and antioxidant enzymes, including AhR-dependent CYP1A1, NQO-1 and AKR1C9, was detected in the rat liver epithelial WB-F344 "stem-like" cells. Additionally, WB-F344 cells express a functional, wild-type form of p53 protein, a biomarker of genotoxic events, and connexin 43, a basic structural unit of gap junctions forming an important type of intercellular communication. In this cellular model, two complementary assays have been established for detection of the modes of action associated with tumor promotion: inhibition of gap junctional intercellular communication (GJIC) and proliferative activity in confluent cells. We found that the PAHs and PCBs, which are AhR agonists, released WB-F344 cells from contact inhibition, increasing both DNA synthesis and cell numbers. Genotoxic effects of some PAHs that lead to apoptosis and cell cycle delay might interfere with the proliferative activity of PAHs. Contrary to that, the nongenotoxic low-molecular-weight PAHs and non-dioxin-like PCB congeners, abundant in the environment, did not significantly affect cell cycle and cell proliferation; however both groups of compounds inhibited GJIC in WB-F344 cells. The release from contact inhibiton by a mechanism that possibly involves the AhR activation, inhibition of GJIC and genotoxic events induced by environmental contaminants are three important modes of action that could play an important role in carcinogenic effects of toxic compounds. The relative potencies to inhibit GJIC, to induce AhR-mediated activity, and to release cells from contact inhibition were determined for a large series of PAHs and PCBs and their metabolites. In vitro bioassays based on detection of events on cellular level (deregulation of GJIC and/or proliferation) or determination of receptor-mediated activities in both ?$stem-like^{\circ}{\times}$ and hepatocyte-like liver cellular models are valuable tools for detection of modes of action of polyaromatic hydrocarbons. They may serve, together with concentration data, as a first step in their risk assessment.

• 한국산림과학회지
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• 제73권1호
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• pp.63-69
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• 1986

설해(雪害)는 일반적으로 돌발적(突發的)으로 발생(發生)하는 재해(災害)로서 지형(地形)이나 환경요인(環境要困)에 따라 많은 영향(影響)을 받게 된다. 본(本) 연구(硏究)는 경남(慶南) 진주지방(晋州地方)에 있어 강우(降雨)에 의(依)한 피해(被害)의 특징(特徵)을 분석(分析)하여 피해(被害)에 관한 기초적(基礎的)인 자료(資料)를 얻고자 수행(遂行)하였든 바 그 결과(結果)를 요약(要約)하연 다음과 같다. 1) 조사지역(調査地域) 2.94ha에서 총입목본수(總立木本數) 14.850 본(本) 중(中) 그 피해목(被害木)은 466 본(本)으로서 약(約) 3.14 %였다. 그 중 설절목(雪折木)은 소나무가 302 본(本), 해공(海松)이 84 본(本), 리기다소나무는 38 본(本), 산오리나무는 1 본(本)으로서 모두 425 본(本)이었고, 설도목(雪倒木)은 소나무가 32 본(本), 해송(海松)이 8 본(本), 리기다소나무가 1 본(本)으로서 모두 41 본(本)이었다. 수종별(樹種別) 피해율(被害率)은 소나무는 5.22%, 해송(海松)은 2.49%, 리기다소나무는 0.92%. 산오리나무는 0.2%였다. 2) 설해(雪害)를 가장 많이 받은 樹高(수고)의 범위는 3~11m 로서 약(約) 95%를 차지하였으며, 흉고직경(胸高直徑)은 3~20cm의 범위에 있는 수종(樹種)이 대부분(大部分)이었다. 3) 피해목(被害木)을 방위별(方位別)로 보면 피해당시(被害當時)의 풍향(風向)에 따라 많은 영향(影響)을 받았으나 사면방향(斜面方向)도 비교적(比較的) 높은 경향(傾向)을 보여주었다. 4) 임령별(林齡別)에 의(依)한 설해(雪害)는 그 폭(幅)이 비교적(比較的) 넓은 편이었으나 11~24년생(年生)의 유령목(幼齡木)이 약(約) 82%로 나타났다. 5) 설해(雪害)로 인한 피해목(被害木)의 절손고비(折損高比) (절손고(折損高)/수고(樹高))를 보면 근원부위(根源部位)는 약(約) 24%, 수간부위(樹幹部位)는 45%, 수관부위(樹冠部位)는 31%로서 비교적(比較的) 수간부위(樹幹部位)의 피해(被害)가 많이 나타나고 있었다. 6) 임목(林木)의 평균형상계수(平均形狀係數)는 1.06으로 나타났으며, 그 피해율(被害率)은 3.14%로서 높은 경향(傾向) 이었다. 따라서 설해(雪害)의 위험(危險)이 있는 임분(林分)은 설해(雪害)의 저항(低抗)을 증진(增進)시킬 수 있는 적절(適切)한 무육방법(撫育方法)과 눈사태 및 융설수(融雪水)에 의(依)한 산붕(山崩), 산사태(山沙汰), 등(等)과 같은 재해(災害)를 예방(豫防)하기 위한 방재대책(防災對策)이 강구(講究)되어야 할 것이다.

• 한국BIM학회 논문집
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• 제6권1호
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• pp.18-24
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• 2016

While the advantages of BIM stem in the automatic and efficient assembling amongst the given, single semantic-embedded objects, it is still unknown the efficiency of BIM for developing the form that has more than one functions. To investigate the research question, in an authentic design course, the participants (N=38) assessed the efficiency of BIM when they designed a shop with the single semantic-embedded objects and multi-semantic objects. Independent T-tests reveal that in BIM, the use of the single semantic-embedded objects is statistically more efficient than that of multi-semantic objects (p<0.01). As the reason, in interviews, the participants reported that they had to split down the planned multi-meanings and assign only one meaning to one form in order to utilize the automatic assembling of BIM. Thus, they spent much effort and time for re-coordinating the match between the forms and multi-semantics. The findings of this study highlight the further directions of BIM in order to suit for the empirical practices of architects.

• Journal of Plant Biology
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• 제17권4호
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• pp.11-17
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• 1974

The present investigation dealt with observation and experiments concerning the growth and differentiation of hop plant, using the varieties of Cascade(C), Shinshuwase(Sh), and Hallertau(H). The results were as follows: (1) Life cycle of hop plants. The annual growth period of hop plant was devided chiefly into 3 phases, dormant, vegetative and reproductive. (2) Growth of main stem. The hop vine begun to grow in the middle of May and grow vigorousely in the middle and latter of June, then gradually decline or stops at the middle of July and the early of August. (3) Growth of lateral vines. By the statistical analysis, it is judged that the varieties of H and Sh were more grown than that of C. H and Sh were not significant, but H and Sh from C were significant in 5% level. (4) Fresh weight and water content of hop cone. Hop cone in fresh weight of C variety was higher than those of other two varieties and water content of hop cone was decreased with time elapse in three races together. (5) Growing point. Histological view of hop varities in each was different. C showed form of sweet potato, H showed form of round, and Sh showed form of ellipse. (6) Shape of the leaf. C and H were 3 lobes, but Sh is 3∼5 lobes. Generally, the color is dark green. (7) Hop cones. Hop cones are as follows.