• Journal of Biomedical Engineering Research
• /
• v.15 no.2
• /
• pp.183-188
• /
• 1994

In clinical orthopaedics, bone resoption in the cortex is often seen post operatively on X-rays or bone densitometry after total hip replacement (THR) in the form of cortical osteoporosis or atropy. Stress shielding of bone occurs, when a load, normally carried by the bone alone, is shared with an implant as a result, the bone stresses are abnormal and with remodelling analysis this may cause extensive proximal bone resoption, possibly weakening the bone bed to the point of failure. The author made finite element models of the cemented and non-cemented type implanted femoral stem with bone resorption of the proximal medial femur and studied the feed back effect of the various degree of bone resoption to THR system by parametric analysis on the stress of the femoral stem and interface. The results of the present finite element analysis implied that the extent of proximal bone resorption has the effect of more increasing stress on the distal stem tip, cement mantle and interface in both type of femoral stem and this high distal stress possibly can cause the mechanical failure of loosening or failure after THR.

• Research in Mathematical Education
• /
• v.24 no.3
• /
• pp.137-173
• /
• 2021

Research and national standards, such as the Next Generation Science Standards (NGSS) in the United States, promote the development and implementation of K-12 interdisciplinary curricula integrating the disciplines of science, technology, engineering, mathematics, and computer science (STEM+CS). However, little research has explored how teachers provide epistemic support in interdisciplinary contexts or the factors that inform teachers' epistemic support in STEM+CS activities. The goal of this paper is to articulate how interdisciplinary instruction complicates epistemic knowledge and resources needed for teachers' instructional decision-making. Toward these ends, this paper builds upon existing models of teachers' instructional decision-making in individual STEM+CS disciplines to highlight specific challenges and opportunities of interdisciplinary approaches on classroom epistemic supports. First, we offer considerations as to how teachers can provide epistemic support for students to engage in disciplinary practices across mathematics, science, engineering, and computer science. We then support these considerations using examples from our studies in elementary classrooms using integrated STEM+CS curriculum materials. We focus on an elementary school context, as elementary teachers necessarily integrate disciplines as part of their teaching practice when enacting NGSS-aligned curricula. Further, we argue that as STEM+CS interdisciplinary curricula in the form of NGSS-aligned, project-based units become more prevalent in elementary settings, careful attention and support needs to be given to help teachers not only engage their students in disciplinary practices across STEM+CS disciplines, but also to understand why and how these disciplinary practices should be used. Implications include recommendations for the design of professional learning experiences and curriculum materials.

• International Journal of Naval Architecture and Ocean Engineering
• /
• v.10 no.6
• /
• pp.794-802
• /
• 2018

The ice resistance series charts for icebreaking ships were developed through a series of systematic model tests in the ice tank of the Korean Research Institute of Ship and Ocean Engineering (KRISO). Spencer's (1992) component-based scaling system for ship-ice model tests was applied to extend the model ship correlations. Beam to draft ratio (B/T), length to beam ratio (L/B), block coefficient ($C_B$) and stem angle (${\alpha}$) were selected as geometric parameters for hull form development. The basic hull form (S1) of twin pod type with B/T of 3.0, L/B of 6.0, $C_B$ of 0.75 and stem angle of $25^{\circ}$ was generated with a modern hull design concept. A total of 13 hulls were designed varying the geometric parameters; B/T of 2.5 and 3.5, L/B of 5.0 and 7.0, $C_B$ from 0.65 to 0.85 in intervals of 0.05, and 5 stem angles from $15^{\circ}$ to $35^{\circ}$. Ice resistance tests were first carried out with the basic hull form in level ice with suitable speed. Four more tests for $C_B$ variations from 0.65 to 0.85 were conducted and two more for beam to draft and length to beam ratios were also performed to study the effect of the geometric parameters on ice resistance. Ice resistance tests were summarized using the volumetric coefficient, $C_V$ ($={\nabla}/L^3$), instead of L/B and $C_B$ variations. Additional model tests were also carried out to account for the effect of the stem angle, ice thickness and ice strength on ice resistance. In order to develop the ice resistance series charts with a minimum number of experiments, the trends of the ice resistance obtained from the experiments were assumed to be similar for other model ship with different geometric parameters. A total of 18 sheets composed of combinations of three different beam to draft ratios and six block coefficients were developed as a parameter of $C_V$ in the low speed regions. Three correction charts were also developed for stem angles, ice thickness and ice strength respectively. The charts were applied to estimate ice resistance for existing icebreaking ships including ARAON, and the results were satisfactory with reasonable accuracy.

• Journal of Korean Society of Forest Science
• /
• v.106 no.4
• /
• pp.480-486
• /
• 2017

This study was conducted to develop the merchantable stem volume models to predict the volume up to upper diameter or upper height out of the total stem volume, targeting on Pinus densiflora, Pinus koraiensis, and Larix kaempferi in South Korea. The 131 stemmed sample trees for stem analysis were used as the data for developing the models. The six kinds of merchantable volume equations including merchantable volume ratio form, ratio form, and exponential ratio form were examined to develop the best models. The two models were finally selected as the best models to predict the merchantable volume: $V_d=V_t\{{\exp}[{\alpha}_1(d^{{\alpha}_2}/D^{{\alpha}_3})]\}$ for upper diameter and $V_h=V_t\{1+{\beta}_1(P^{{\beta}_2}/H^{{\beta}_3})\}$ for upper height. By rearranging the best model equations, implicit taper functions were derived, and the estimation was performed for the upper height by upper diameter and upper diameter by upper height. Because of not only the high accuracy but also the convenience, the models developed in this study were considered to be easily applicable in the field of forestry.

• Journal of Korean Society of Forest Science
• /
• v.83 no.4
• /
• pp.521-530
• /
• 1994

Voume functions, which are usually expressed by the function of dbh and height, are estimated commonly through the regression analysis with the highest statistical accuracy considered. In Korea, general volume functions for each tree species were prepared by means of the regression analysis with the exponential function ($V=aD^bH^c$) having the dbh(D) and height(H) as independent variables. In this study, regional stem curve functions for the Pinus densiflora in Kangwon-province were derived and a regional volume function model, in which the stem volume can be directly estimated through the rotational integral of the regional stem curve functions, was prepared. The regional volume estimated by the prepared model was more accurate than the volume by the general volume table for the Pinus densiflora in Kangwon-province. Additionary, the form of stem curves derived by the regional stem curve functions showed difference from each other. The stem in Youngwol and Wonju taper down more fast in upper part than that in other regions. These various stem forms also led to the regional difference in volume estimates.

• Journal of the Korean Wood Science and Technology
• /
• v.44 no.6
• /
• pp.903-912
• /
• 2016

Morphological and anatomical evaluation of grafted P. merkusii have been undertaken to obtain the information about compatible and incompatible symptoms of 18 years old grafts based on morphological observation and microscopic analysis. Samples of compatible and incompatible grafts were obtained from previous research conducted by the Silviculture Departement Team in 1994. Result showed that compatible grafts have normal stem form and secondary growth (diameter growth), but some abnormality symptoms like undulated pattern of annual growth rings, phloem thickening and abnormality resin ducts in inner and middle parts of the union area occurred. Incompatible ones showed abnormality of the stem form, cortex-bark necrosis and swelling in the union area. Microscopic observation showed abnormality of all parts of the union, undulated pattern of annual growth rings, phloem thickening, abnormal resin ducts, low numbers and discontinuity of vascular elements in the union area.

• BMB Reports
• /
• v.52 no.12
• /
• pp.689-694
• /
• 2019

Ethical and safety issues have rendered mesenchymal stem cells (MSCs) popular candidates in regenerative medicine, but their therapeutic capacity is lower than that of induced pluripotent stem cells (iPSCs). This study compared original, dental tissue-derived MSCs with re-differentiated MSCs from iPSCs (iPS-MSCs). CD marker expression in iPS-MSCs was similar to original MSCs. iPS-MSCs expressed higher in pluripotent genes, but lower levels in mesodermal genes than MSCs. In addition, iPS-MSCs did not form teratomas. All iPSCs carried mtDNA mutations; some shared with original MSCs and others not previously detected therein. Shared mutations were synonymous, while novel mutations were non-synonymous or located on RNA-encoding genes. iPS-MSCs also harbored mtDNA mutations transmitted from iPSCs. Selected iPS-MSCs displayed lower mitochondrial respiration than original MSCs. In conclusion, screening for mtDNA mutations in iPSC lines for iPS-MSCs can identify mutation-free cell lines for therapeutic applications.

• Biomedical Science Letters
• /
• v.15 no.1
• /
• pp.37-45
• /
• 2009

Insect cell cultures have become important tools in the production of biological substances for use in a variety of research, human and veterinary medicine, and pest control applications. These applications often require the introduction of foreign DNA into the cells and have generally used methods originally developed for use with human and other mammalian cell cultures. While these methods can be successfully employed, they are often less efficient with insect cells and frequently involve complex procedures or require specialized equipment. Even when they do work, they may require substantial modification because of differences in the culture medium or growth patterns of insect cells. In this study, We have optimized transfection conditions of Sf9 cell line using insect expression vector pIZT/V5-His which expresses green fluorescent protein effectively. Human stem cell factor (hSCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. It can exist in membrane-bound form and in proteolytically released soluble form. As determined by an enzyme-linked immunosorbent assay performed, hSCF level in supernatant averaged 995ng/ml. The human hSCF was partially purified by immunoaffinity chromatography and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the hSCF has N-linked carbohydrate and corresponds to the soluble form, at or about 223 amino acids in length. The findings suggest functional importance for soluble hSCF in cells.

• Journal of Industrial Technology
• /
• v.42 no.1
• /
• pp.29-36
• /
• 2022

The CRISPR system has revolutionized gene editing field. Cas9-mediated gene editing such as Indel induction or HDR enable targeted gene disruption or precise correction of mutation. Moreover, CRISPR-based new editing tools have been developed such as base editors. In this review, we focus on gene editing in human pluripotent stem cells, which is principal technique for gene correction therapy and disease modeling. Pluripotent stem cell-specific drug YM155 enabled selection of target gene-edited pluripotent stem cells. Also, we discussed base editing for treatment of congenital retina disease. Adenine base editor delivery as RNP form provide an approach for genetic disease treatment with safe and precise in vivo gene correction.

• Korean Journal of Veterinary Service
• /
• v.38 no.3
• /
• pp.205-209
• /
• 2015

A 7 years old, female and neutered ferret (Mustela putorius furo) was presented to local animal hospital due to lethargy, anorexia and hypothermia. Radiography, ultrasonography and blood test were performed. On the basis of clinical signs and tests, this case was presumed to be lymphoma. In consideration of this ferret's condition, chemotherapy was carried out for the treatment. However, the ferret died a few days. After necropsy, metastatic tumors were observed over abdomen. All tumors were packed with homologous lymphocytes with pleomorphic changes and numerous mitotic figures. Consequently, this masses were diagnosed as B-cell gastrointestinal lymphoma, which were negative for CD3 on immunohistochemistry.