• Korean Journal of Microbiology
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• v.40 no.4
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• pp.334-341
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• 2004

Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.

• Journal of the Korean Recycled Construction Resources Institute
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• v.8 no.4
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• pp.520-527
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• 2020

Hwangtoh is the rich resource that accounts for about 15.0% of the domestic soil, and can be used as the admixture of concrete with Pozzolan characteristics if activated by rapidly freezing after burning with high temperature. In this study, the mortar curbs containing active hwangtoh were produced, based on the mixture for the mortar curbs sold on the market. The substitution rate of active hwangtoh were considered 10.0% and 25.0%, and the test items were selected to compressive and flexural strength tests, freezing/thawing resistance tests, accelerated carbonation tests, and accelerated chloride diffusion tests. In the results of the mechanical performance, it was showed that the highest strength was evaluated in OPC mixture, and the increase in strength was small by the increase of age, which was believed to be due to the fact that most of the strength in each mixture was created in three days of steam curing. The results of the freezing/thawing tests for 28 aged days showed the reduction rate of compressive strength was 85.0% or higher for all specimen, meeting the criteria presented. The accelerated carbonation tests were carried out on the specimen at 28 days of age, and the results showed that the mortar with active hwangtoh had lower carbonation resistance performance than mortar with OPC. The passed charge of each mixture was assessed in accordance with ASTM C 1202 on 28 and 91 aged days. The OPC mixture had "Low" rate and the mortar with active hwangtoh had "Moderate" rate. So it was thought that the mortar with active hwangtoh had appropriate resistance performance for chloride attack.