• Title/Summary/Keyword: Steady-state operation

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Development of Continuous Culture Process for Economic Production of Hyaluronic Acid (HA) Biosynthesized by Streptococcus zooepidemicus (Streptococcus zooepidemicus 유래 히알루론산의 경제적 생산을 위한 연속배양 공정 개발)

  • Kim, Soo Yeon;Chun, Gie-Taek
    • Microbiology and Biotechnology Letters
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    • v.48 no.4
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    • pp.525-532
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    • 2020
  • A continuous fermentation process was carried out to enhance hyaluronic acid (HA) production using Streptococcus zooepidemicus cells. During the 1st stage continuous operation from 8 h with a dilution rate of 0.029/h (D1), HA was produced in the range of 7.5-10 g/l. During the 2nd stage from 44 h with a dilution rate of 0.036/h (D2), HA production (8.28 g/l) was initially reduced to a small extent due to increase of dilution rate from D1 to D2, and then a new pseudo-steady state was formed within a few hours with a concurrent small variations of HA production. The HA amount produced during the latter part of the 2nd stage was stably maintained in the range of 8.28-9.48 g/l, about 4.7% less amount compared to the 1st stage. Due to 24% increase of dilution rate from D1 to D2, however, maximum volumetric productivity (DP) amounting to 0.341 g/l/h was obtained at 96 h during the 2nd stage. This maximum productivity obtained from the continuous culture turned out only a small increase (3%) as compared to the corresponding batch fermentation. However, it should be noted that, in the case of batch process, one run typically consists of serial stages of growth culture plus one final production culture. This implies that, if the continuous fermentation that practically needs no dead time necessary for the multi-stage growth cultures is run for longer period, the total amount of the accumulated HA would be far greater than the amount obtained from the corresponding batch culture performed for the identical period.

"Legal Study on Boundary between Airspace and Outer Space" (영공(領空)과 우주공간(宇宙空間)의 한계(限界)에 관한 법적(法的) 고찰(考察))

  • Choi, Wan-Sik
    • The Korean Journal of Air & Space Law and Policy
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    • v.2
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    • pp.31-67
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    • 1990
  • One of the first issues which arose in the evolution of air law was the determination of the vertical limits of airspace over private property. In 1959 the UN in its Ad Hoc Committee on the Peaceful Uses of Outer Space, started to give attention to the question of the meaning of the term "outer space". Discussions in the United Nations regarding the delimitation issue were often divided between those in favour of a functional approach ("functionalists"), and those seeking the delineation of a boundary ("spatialists"). The functionalists, backed initially by both major space powers, which viewed any boundary as possibly restricting their access to space(Whether for peaceful or military purposes), won the first rounds, starting with the 1959 Report of the Ad Hoc Committee on the Peaceful Uses of Outer Space which did not consider that the topic called for priority consideration. In 1966, however, the spatialists, were able to place the issue on the agenda of the Outer Sapce Committee pursuant to Resolution 2222 (xxx1). However, the spatialists were not able to present a common position since there existed a variety of propositions for delineation of a boundary. Over the years, the funtionalists have seemed to be losing ground. As the element of location is a decisive factor for the choice of the legal regime to be applied, a purely functional approach to the regulation of activities in the space above the Earth does not offer a solution. It is therefore to be welcomed that there is clear evidence of a growing recognition of the defect inherent to such an approach and that a spatial approach to the problem is gaining support both by a growing number of States as well as by publicists. The search for a solution of the problem of demarcating the two different legal regimes governing the space above the Earth has undoubtedly been facilitated, and a number of countries, among them Argentina, Belgium, France, Italy and Mexico have already advocated the acceptance of the lower boundary of outer space at a height of 100km. The adoption of the principle of sovereignty at that height does not mean that States would not be allowed to take protective measures against space activities above that height which constitute a threat to their security. A parallel can be drawn with the defence of the State's security on the high seas. Measures taken by States in their own protection on the high seas outside the territorial waters-provided that they are proportionate to the danger-are not considered to infringe the principle of international law. The most important issue in this context relates to the problem of a right of passage for space craft through foreign air space in order to reach outer space. In the reports to former ILA Conferences an explanation was given of the reasons why no customary rule of freedom of passage for aircraft through foreign territorial air space could as yet be said to exist. It was suggested, however, that though the essential elements for the creation of a rule of customary international law allowing such passage were still lacking, developments apperaed to point to a steady growth of a feeling of necessity for such a rule. A definite treaty solution of the demarcation problem would require further study which should be carried out by the UN Outer Space Committee in close co-operation with other interested international organizations, including ICAO. If a limit between air space and outer space were established, air space would automatically come under the regime of the Chicago Convention alone. The use of the word "recognize" in Art. I of chicago convention is an acknowledgement of sovereignty over airspace existing as a general principle of law, the binding force of which exists independently of the Convention. Further it is important to note that the Aricle recognizes this sovereignty, as existing for every state, holding it immaterial whether the state is or is not a contracting state. The functional criteria having been created by reference to either the nature of activity or the nature of the space object, the next hurdle would be to provide methods of verification. With regard to the question of international verification the establishment of an International Satelite Monitoring Agency is required. The path towards the successful delimitation of outer space from territorial space is doubtless narrow and stony but the establishment of a precise legal framework, consonant with the basic principles of international law, for the future activities of states in outer space will, it is still believed, remove a source of potentially dangerous conflicts between states, and furthermore afford some safeguard of the rights and interests of non-space powers which otherwise are likely to be eroded by incipient customs based on at present almost complete freedom of action of the space powers.

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Numerical Studies of a Separator for Stack Temperature Control in a Molten Carbonate Fuel Cell (용융탄산염 연료전지 스택 온도 조절을 위한 분리판에 관한 수치 해석 연구)

  • Kim, Do-Hyung;Kim, Beom-Joo;Lim, Hee-Chun
    • Transactions of the Korean hydrogen and new energy society
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    • v.22 no.3
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    • pp.305-312
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    • 2011
  • The use of a separator to control stack temperature in a molten carbonate fuel cell was studied by numerical simulation using a computational fluid dynamics code. The stack model assumed steady-state and constant-load operation of a co-flow stack with an external reformer at atmospheric pressure. Representing a conventional cell type, separators with two flow paths, one each for the anode and cathode gas, were simulated under conditions in which the cathode gas was composed of either air and carbon dioxide (case I) or oxygen and carbon dioxide (case II). The results showed that the average cell potential in case II was higher than that in case I due to the higher partial pressures of oxygen and carbon dioxide in the cathode gas. This result indicates that the amount of heat released during the electrochemical reactions was less for case II than for case I under the same load. However, simulated results showed that the maximum stack temperature in case I was lower than that in case II due to a reduction in the total flow rate of the cathode gas. To control the stack temperature and retain a high cell potential, we proposed the use of a separator with three flow paths (case III); two flow paths for the electrodes and a path in the center of the separator for the flow of nitrogen for cooling. The simulated results for case III showed that the average cell potential was similar to that in case II, indicating that the amount of heat released in the stack was similar to that in case II, and that the maximum stack temperature was the lowest of the three cases due to the nitrogen gas flow in the center of the separator. In summary, the simulated results showed that the use of a separator with three flow paths enabled temperature control in a co-flow stack with an external reformer at atmospheric pressure.

Effect of Low Calorific Natural Gas on Performance and Emission Characteristics of Engine (저발열량 천연가스가 엔진 성능 및 배기특성에 미치는 영향)

  • Lee, Sungwon;Lim, Gihun;Park, Cheolwoong;Choi, Young;Kim, Changgi
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.12
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    • pp.1129-1135
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    • 2013
  • In this, three types of natural gas were employed to investigate the effect of low-calorific natural gas on the performance of and emissions from a heavy-duty CNG engine. The performance and emission characteristics were analyzed by conducting a full-load test, WHSC mode test, and WHTC mode test. The results showed that the torque of low-calorific natural gas with $9,800kcal/Nm^3$ of higher heating value decreased by 4.4 compared to that of the current natural gas with $10,400kcal/Nm^3$ of heating value. With low-calorific fuels, CO, $CO_2$, and $NO_x$ emissions decreased. However, THC emissions increased. According to the WHSC and WHTC mode test results, the thermal efficiency increased and the emission characteristics showed a similar trend to the full-load test results. Low-calorific natural gases cause a decrease in torque at full-load operation conditions and an increase in hydrocarbon emissions.

Treating Swine Wastewater by Anaerobic Bioreactors (혐기성 생물반응기에 의한 축산폐수의 처리)

  • Lee, Gook-Hee;Kim, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.18 no.1
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    • pp.54-60
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    • 1999
  • Three different types of lab-scale anaerobic bioreactors, AF and two-stage ASBF-PR and ASBF-SP, were evaluated in treating swine wastewater by operating at $1{\sim}2$ days of hydraulic retention time with increasing organic loading rate upto 6.3 $kg-COD/m^3{\cdot}d$ at $35^{\circ}C$. Seeding the anaerobic bioreactors with waste anaerobic digester sludge from a municipal wastewater treatment plant was effective and a 40-day acclimation period was required for steady-state operation. Three anaerobic bioreactors were effective in treating swine wastewater with COD removal efficiency of $66.4{\sim}84.9$% and biogas production rate of $0.333{\sim}0.796m^3/kg-COD_{removed}{\cdot}d$. Increases of organic loading rate by increasing influent COD concentration and/or decreasing hydraulic retention time caused decreases in COD removal efficiency and increases in biogas production rate. At relatively high organic loading rate employed in this study, the treatment efficiency of AF and ASBF-PR were similar but superior than that of ASBF-SP, indicating that porosity and pore size of the media packed in the bioreactors are more important factors contributing the performance of to bioreactors than specific surface area of the media. TKN in swine wastewater must be removed prior to the anaerobic processes when anaerobic process is considered as a major treatment process since influent TKN concentration of $1,540{\sim}1,870mg/L$ to the bioreactors adversely affect the activity of methanogenic bacteria, resulting in decreases of treatment efficiency and biogas production rate by 50%.

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Rotordynamic Analysis of a Dual-Spool Turbofan Engine with Focus on Blade Defect Events (블레이드 손상에 따른 이축식 터보팬 엔진의 동적 안정성 해석)

  • Kim, Sitae;Jung, Kihyun;Lee, Junho;Park, Kihyun;Yang, Kwangjin
    • Tribology and Lubricants
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    • v.36 no.2
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    • pp.105-115
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    • 2020
  • This paper presents a numerical study on the rotordynamic analysis of a dual-spool turbofan engine in the context of blade defect events. The blades of an axial-type aeroengine are typically well aligned during the compressor and turbine stages. However, they are sometimes exposed to damage, partially or entirely, for several operational reasons, such as cracks due to foreign objects, burns from the combustion gas, and corrosion due to oxygen in the air. Herein, we designed a dual-spool rotor using the commercial 3D modeling software CATIA to simulate blade defects in the turbofan engine. We utilized the rotordynamic parameters to create two finite element Euler-Bernoulli beam models connected by means of an inter-rotor bearing. We then applied the unbalanced forces induced by the mass eccentricities of the blades to the following selected scenarios: 1) fully balanced, 2) crack in the low-pressure compressor (LPC) and high pressure compressor (HPC), 3) burn on the high-pressure turbine (HPT) and low pressure compressor, 4) corrosion of the LPC, and 5) corrosion of the HPC. Additionally, we obtained the transient and steady-state responses of the overall rotor nodes using the Runge-Kutta numerical integration method, and employed model reduction techniques such as component mode synthesis to enhance the computational efficiency of the process. The simulation results indicate that the high-vibration status of the rotor commences beyond 10,000 rpm, which is identified as the first critical speed of the lower speed rotor. Moreover, we monitored the unbalanced stages near the inter-rotor bearing, which prominently influences the overall rotordynamic status, and the corrosion of the HPC to prevent further instability. The high-speed range operation (>13,000 rpm) coupled with HPC/HPT blade defects possibly presents a rotor-case contact problem that can lead to catastrophic failure.

A Performance Improvement of SE-MMA Adaptive Equalization Algorithm using Adaptive Varying Modulus (Adaptive Varying Modulus를 이용한 SE-MMA 적응 등화 알고리즘의 성능 개선)

  • Lim, Seung-Gag
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.1
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    • pp.79-84
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    • 2018
  • This paper relates with the performance improvement of SE-MMA (Signed Error-Multiple Modulus Algorithm) adaptive equalization algorithm that is used for the reduction of the intersymbol interference due to the distortion which occurs in the communication channel for the transmission of 16-QAM nonconstant modulus signal.. In the conventional MMA, the fixed modulus value that is second order statistics of transmitting signal were used, and the SE-MMA was introduced in order to the simplification of the algorithm's arithmetic operation. The SE-MMA have a fast convergence speed than MMA, but it has a problem of degradation of equalization performance in the steady state due to the arithmetic simplification. In this paper, we propose the new algorithm AV-SE-MMA (Adaptively Varying-SE-MMA) that uses the adaptive varying modulus in order to obtain the error signal for updating the adaptive equalizer coefficient, and its equalization performance were confirmed by simulation. In this paper, the performance of SE-MMA and proposed algorithm were compared, and the equalizer output signal constellation, residual isi, MSE and SER in order to confirm the robustness of noise were used as performace index. As a result of performance comparison, the AV-SE-MMA has better performance in output signal constellation, residual isi and MD compared to the SE-MMA, but it was confirmed that the AV-SE-MMA has similar in the SER performance that means the robustness to the noise.

Biofilter Treatment of Waste Air Containing Malodor and VOC: 1. Pressure Drop and Microbe-population Distribution of Biofilter with Improved Design (악취 및 VOC를 함유한 폐가스의 바이오필터 처리: 1. 개선된 바이오필터설계에 의한 압력강하와 미생물 population 분포)

  • Lee, Eun Ju;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.51 no.1
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    • pp.127-135
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    • 2013
  • In this study, both pressure drop and microbe-population-distribution were observed while running a novel biofilter system with improved design in which the biofilter system is composed of two, upper and lower biofilters with both equal feed-rates of up-flow and down-flow, respectively. Then they were compared with the pressure drop and microbe-population-distribution observed in a conventional biofilter of the same effective volume with unidirectional flow. The pressure drop-value of biofilter system with improved design turned out to be less at the incipient stage of run or steady-state long term operation by more than 40~80% of that of the conventional biofilter. The microbe-population-distribution was observed to be lower and higher at higher and lower column of biofilter, respectively, for both the conventional biofilter and the biofilter system with improved design. The microbe-media of waste-tire crumb showed much greater CFU counts than GAC. In the biofilter system with improved design, the $bottom{\rightarrow}up$ feeding of waste air showed greater microbe-population growth than the $top{\rightarrow}down$ feeding for both the microbe-media of waste-tire crumb and GAC. However, it was more prominent for the former than the latter. Comparing the microbe-population-distributions of both of the conventional biofilter and the biofilter system with improved design, the microbe-population of latter was distributed ca. 15 and 2.5 times more evenly for GAC and the media of waste-tire crumb, respectively, than that of former.

A Study on the Anaerobic Treatment of the Phenol-bearing Wastewater with two Sludge Blanket-Packed Bed Reactors in Series (2단의 슬러지-고정상 반응기에서 페놀 함유 폐수의 혐시성 처리에 관한 연구)

  • 정종식;안재동;박동일;신승훈;장인용
    • Journal of Environmental Health Sciences
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    • v.21 no.4
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    • pp.1-9
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    • 1995
  • This study was carried to investigate the biodegradability of phenol in the wastewater with the two sludge blanket-packed bed reactor in series. Each reactor had a dimension of 0.09 m i.d. and 1.5 m height and consisted of two regions. The lower region was a sludge blanket of 0.5 m height and the upper region was a packed-bed of 1 m height. The packed bed region was charged with ceramic raschig rings of 10 mm i.d., 15 mm o.d. and 20 mm length. The reactors were operated at 35$\circ$C and the hydraulic retention time(HRT) was maintained 24 hours. The synthetic wastewater composed of glucose and phenol as major components was fed into the reactor in a continuous mode with incereasing phenol concentration. In addition, the nutrient trace metals($Na^+, Mg^{2+}, Ca^{2+}, PO_4^{3-}, NH_4^+, Co^{2+}, Fe^{2+}$ etc.) were added for growing anaerobes. The phenol concentration of the effluent, the overall gas production, the composition of product gas, the efficiency of COD reduction and the duration of acclimation period were measured to determine the performance of the anaerobic wastewater treatment system as the phenol concentration of the influent was increased from 600 to 2400 mg//l. Successfully stable biodegradation of phenol could be achieved with the anaerobic treatment system from 600 to 1, 800 mg/l of the influent phenol concentration. The upper level of influent phenol loading was high enough to meet most of the practical requirement. The duration of acclimation increased with the phenol loading. At steady state of the influent phenol concentration of 1800 mg/l, the treatment performance indicated the phenol reduction efficiency of 99%, the COD reduction efficiency of 99% and the gas production rate of 37 l/day. At the influent phenol concentration of 2400 mg/l, however, the operation of the treatment system was noted unstable. While the concentration of methane in biogas decreased with increasing the influent phenol loading, the carbon dioxide was increased. However, the concentration of hydrogen was varied negligibly. The concentration of methane was high enough to be used as a fuel. As a result, it is suggested that anaerobic phenol wastewater treament was economical in the sense of energy recovery and wastewater treatment.

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Performance Evaluation of SE-MMA Adaptive Equalization Algorithm with Varying Step Size based on Error Signal's Nonlinear Transform (오차 신호의 비선형 변환을 이용한 Varying Step Size 방식의 SE-MMA 적응 등화 알고리즘의 성능 평가)

  • Lim, Seung-Gag
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.17 no.1
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    • pp.77-82
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    • 2017
  • This paper related with the VSS_SE-MMA (Varying Step Size_Signed Error-MMA) which possible to improving the equalization performance that employing the varying adaptive step size based on the nonlinearities of error signal of SE-MMA (Signed Error-MMA), compensates the intersymbol interference by distortion occurs at the communication channel, in the transmitting the spectral efficient nonconstant modulus signal such as 16-QAM. The SE-MMA appeared to the reducing the computational arithematic operation using the polarity of error signal in the updating the tap coefficient of present MMA adaptive equalizer, but have a problem of equalization performance degradation. The VSS_SE-MMA improves the problem of such SE-MMA, using the varying step size consider the error signal in the update the adaptive equalizer tap coefficient, and its improved performance were confirmed by simulation. For this, the output signal constellation of equalizer, the residual isi and maximum distortion, MSE and SER were applied. As a result of computer simulation, it was confirmed that the VSS_SE-MMA algorithm has nearly same in convergence speed and has more good performance in every performance index at the steady state.