• Title/Summary/Keyword: Starvation

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Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak

  • Yu, Xiaoqiang;Peng, Quanhui;Luo, Xiaolin;An, Tianwu;Guan, Jiuqiang;Wang, Zhisheng
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.11
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    • pp.1593-1600
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    • 2016
  • This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak ($110.3{\pm}5.85kg$) were randomly divided into two groups (baseline and starvation group). The yak of baseline group were slaughtered at day 0, while the other group of yak were kept in shed without feed but allowed free access to water, salt and free movement for 9 days. Blood samples of the starvation group were collected on day 0, 1, 2, 3, 5, 7, 9 and the starved yak were slaughtered after the final blood sample collection. The liver and muscle glycogen of the starvation group decreased (p<0.01), and the lipid content also decreased while the content of moisture and ash increased (p<0.05) both in Longissimus dorsi and liver compared with the baseline group. The plasma insulin and glucose of the starved yak decreased at first and then kept stable but at a relatively lower level during the following days (p<0.01). On the contrary, the non-esterified fatty acids was increased (p<0.01). Beyond our expectation, the ketone bodies of ${\beta}$-hydroxybutyric acid and acetoacetic acid decreased with prolonged starvation (p<0.01). Furthermore, the mRNA expression of lipogenetic enzyme fatty acid synthase and lipoprotein lipase in subcutaneous adipose tissue of starved yak were down-regulated (p<0.01), whereas the mRNA expression of lipolytic enzyme carnitine palmitoyltransferase-1 and hormone sensitive lipase were up-regulated (p<0.01) after 9 days of starvation. The phosphoenolpyruvate carboxykinase and pyruvate carboxylase, responsible for hepatic gluconeogenesis were up-regulated (p<0.01). It was concluded that yak derive energy by gluconeogenesis promotion and fat storage mobilization during starvation but without ketone body accumulation in the plasma.

The Effect of Oil-Starvation on the Lubrication Characteristics of High-Speed Bearing: Part II-Roller Bearing (가스터어빈용 고속 베어링의 Oil-Starvation 윤활특성: Part II-Roller Bearing)

  • Kim, Ki-Tae
    • Tribology and Lubricants
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    • v.13 no.1
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    • pp.76-81
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    • 1997
  • The lubrication characteristics of high-speed roller bearings at oil-starvation have been investigated empirically using the bearings employed in small industrial gas turbine engines. Testing was done by simulating the oil-starvation conditions in engines, such as stopping the oil-supply to the bearing during normal operating, starting without oil-supply at atmospheric temperature, and accelerating with oil-supply at atmospheric temperature. During testing, the temperature of bearing, the power consumption, and the rotating resistance of the bearing were measured. From this study, on the contrary to the ball bearing, it was found that the resistance of the bearing was higher at the regime of without oil-supply than that at the regime of with oil-supply, despite less power consumption.

The Expression of Hsp70 and GST Genes in Mytilus coruscus during Air Exposure and Starvation (공기노출 및 절식시기 동안 참담치, Mytilus coruscus 에서 Hsp70 및 GST 유전자 발현에 대한 연구)

  • Kim, Chul Won;Kang, Han Seung
    • The Korean Journal of Malacology
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    • v.32 no.2
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    • pp.73-81
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    • 2016
  • Heat shock proteins (HSPs), one of the most highly conserved groups of proteins characterized to date, play crucial roles in protecting cells against environmental stresses, such as heat shock, salinity and oxidative stress. The glutathione S-transferases (GST) have important role in detoxification of oxidative stress, environmental chemicals and environmental stress. GST mRNA expression have been used as biomarkers on environmental stress. The purpose of this study was to investigate the death rate and the gene expression of Hsp70 and GST during air exposure and starvation. Results showed that, the expression of Hsp70 mRNA was significantly changed in the experiment groups, such as air exposure and starvation. GST mRNA expression was significantly increased in the experimental group of starvation. These results suggest that Hsp70 and GST were played roles in biomarker gene on the air exposure and starvation.

Effect of Sugar Starvation on the Sugar Transport System in Suspension Cultures of Streptanthus trotus (Streptanthus tortus 배양 세포에서 당류고갈이 당류 수송계에 미치는 영향)

  • 조봉희
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.47-50
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    • 2000
  • In suspension cultures of Streptanthus. the uptake rate of sugar was increased during the ceil starvation of sugar in the medium. The maximal uptake rate obtained with 3 days of cell starvation. Sugar transport system induced by the sugar starvation was completely inhibited by 10 $\mu$M cycloheximide. Plant cells are known to possess only one sugar transport system, but the uptake rate of glucose obtained a saturated kinetic while the one of sucrose had two different kinetics after the sugar starvation. Induced sugar transport systems had different kinetics compared to plant cell. These results showed that higher plants have adaptable ability to induce new sugar transport systems when the environment changed unsuitable.

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Effect of Bojungikgitang on Starvation Stress in Mice (보중익기탕(補中益氣湯)이 생쥐의 기아(飢餓)stress에 미치는 영향)

  • Kim, Jung-Hoon;Lee, Tae-Hee
    • Herbal Formula Science
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    • v.14 no.1
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    • pp.133-140
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    • 2006
  • Objectives: We investigated the effect of Bojungikgitang on the change of the corticosterone and the rectal temperature of the mice induced by starvation stress. Methods: After administration of Bojungikgitang three times, mice were starved. The corticosterone and rectal temperature were measured after 36.5 hours starvation stress. Results : The plasma corticosterone level in Bojungikgitang(0.25g/Kg) was decreased comparing with the Control group and the rectal temperature in the Bojungikgitang(1.0g/Kg) was increased comparing with the Control group. Conclusion : it might be recognized that Bojungikgitang has anti starvation stress effect, and also it might be needed further study in various viewpoints.

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The Effects of Starvation on Physiological Changes and Stress Response in Cultured Cobitid Loach (Misgurnus anguillicaudatus) Exposed to Sodium Nitrite

  • Hur, Jun Wook;Gil, Hyun Woo;Park, In-Seok
    • Journal of Marine Life Science
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    • v.3 no.2
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    • pp.87-95
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    • 2018
  • To investigate effects of starvation on physiological changes, stress response, and survival of cobitid loach (Misgurnus anguillicaudatus) exposed to sodium nitrite (NaNO2), a 4-week experiment was conducted. Fewer fish survived in the starved group than those in the fed group during the experiment. Starvation resulted in growth retardation, leading to differences in body length and body depth between fed and starved groups. The fed gorup continued to grow and remained in good condition. Blood chemical analysis (plasma cortisol and glucose) showed significant differences in stress response to nitrite exposure between fed and starved groups (p < 0.05). These results suggest that all parameters employed in this study to assess effects of starvation with NaNO2 stress are useful information for researching nutritional status in cobitid loach.

A Study to Simulate Cell Voltage-Reversal Behavior Caused by Local Hydrogen Starvation in a Stack of Fuel Cell Vehicle (연료전지차 스택 내 국부적 수소 부족에 기인한 셀 역전압 거동 모사에 대한 연구)

  • Park, Ji Yeon;Im, Se Joon;Han, Kookil;Hong, Bo Ki
    • Transactions of the Korean hydrogen and new energy society
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    • v.24 no.4
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    • pp.311-319
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    • 2013
  • A clear understanding on cell voltage-reversal behavior due to local hydrogen starvation in a stack is of paramount importance to operate the fuel cell vehicle (FCV) stably since it affects significantly the cell performance and durability. In the present study, a novel experimental method to simulate the local cell voltage-reversal behavior caused by local hydrogen starvation, which typically occurs only one or several cells out of several hundred cells in a stack of FCV, has been proposed. Contrary to the conventional method of overall fuel starvation, the present method of local hydrogen starvation caused the local cell voltage-reversal behavior in a stack very well. Degradation of both membrane electrode assembly (i.e., pin-hole formation) and gas diffusion layer due to an excessive exothermic heat under voltage-reversal condition was also observed clearly.

Degradation of Trichloroethylene by a Growth-Arrested Pseudomonas putida

  • Hahm, Dae-Hyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.1
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    • pp.11-14
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    • 1998
  • A toluene-oxidizing strain of Pseudomanas mendocina KR1 containing toluene-4-mono-oxygenase (TMO) completely degrades TCE with the addition of toluene as a co-substrate in aerobic condition. In order to construct in situ bioremediation system for TCE degradation without any growth-stimulating nutrients or toxic inducer such as toluene, we used the carbon-starvation promoter of Pseudomonas putida MK1 (Kim, Y. et al., J. bacteriol., 1995). Upon entry into the stationary phase due to the deprivation of nutrients, this promoter is strongly induced without further cell growth. The TMO gene cluster (4.5 kb) was spliced downstream of the carbon starvation promoter of Pseudomonas putida MK1, already cloned in pUC19. TMO under the carbon starvation promoter was not expressed in E. coli cells either in stationary phase or exponential phase. For TMO expression in Pseudomonas strains, tmo and carbon starvation promoter region were recloned into a modified broad-host range vector pMMB67HES which was made from pMMB67HE(8.9 kb) by deletion of tac promoter and lacIq (about 1.5 kb). Indigo was produced by TMO under the carbon starvation promoter in a Pseudomonas strain of post-exponential phase on M9 (0.2% glucose and 1mM indole) or LB. 18% of TCE was degraded in 14 hours after entering the stationary phase at the initial concentration of 6.6 ${\mu}$M in liquid phase.

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Effectiveness of Zeolite and Granular Activated Carbon Addition before Starvation for the Performance Recovering of the Sludge Settleability and Removal Efficiency (Starvation전 제올라이트 및 입상활성탄의 주입이 슬러지 침강성 및 오염물질 처리효율 회복에 미치는 영향)

  • Oh, Hye-Ran;Kim, Sang-Soo;Moon, Byung-Hyun;Yoon, Cho-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.3
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    • pp.234-240
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    • 2010
  • The effectiveness of adding powdered zeolite and granular activated carbon (GAC) before starvation into biological reactor for recovering its performances was investigated. Two types of carrier addition in Sequencing Batch Reactor (SBR) system for non-saline and saline wastewater were evaluated after starvation periods. During the experiment, settleablity (SVI), floc size, fractal dimension, $COD_{Mn}$, T-N, T-P removal efficiencies and recovery time were monitored. When the wastewater feeding was resumed after starvation period for 5days, the SVI increased at the beginning of resumption and then decreased with time in both types. And the larger the floc size and fractal dimension of floc, the more increased removal efficiency for $COD_{Mn}$, T-N and T-P was also. Its performance recovery was strongly correlated with floc size and fractal dimension of activated sludge. After resuming the wastewater feeding, the SVI, floc size, fractal dimension, $COD_{Mn}$, T-N, T-P removal efficiency of SBR with carrier improved and reached its initial value faster compared to those of SBR without carrier.

The Effects of Phosphate Starvation on the Activities of Acid and Alkaline Phosphatase, Fructose-1,6-bisphosphatase, Sucrose-phosphate Synthase and Nitrate Reductase in Melon (Cucumis melo L.) Seedlings

  • Kang, Sang-Jae;Lee, Chang-Hee;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.1
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    • pp.44-52
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    • 2016
  • Plants response to phosphate starvation include the changes of activity of some enzymes, such as phosphatases, fructose-1,6-bisphosphatase, sucrose-phosphate synthase and nitrate reductase. In this study, to determine the effects of phosphate starvation on the change of activities of acid and alkaline phosphatase, fructose-1,6-bisphosphatase, sucrose-phosphate synthase, and nitrate reductase were studied in melon seedlings (Cucumis melo L.). The content of the protein and chlorophyll tended to relatively reduced in melon seedlings subjected to phosphate starvation. Acid phosphatase activity in first and second leaves of melon seedlings was relatively higher than that of third and fourth leaves of seedlings in 14 days after phosphate starvation treatment, respectively. Active native-PAGE band patterns of acid phosphatase in melon leaves showed similar to activities of acid phosphatase, whereas alkaline phosphatase activity was different from the change in the activity of acid phosphatase. Inorganic phosphate content in melon seedlings leaves was constant. The changes of Fructose-1,6-bisphosphatase and sucrose phosphate synthase activities showed similar patterns in melon seedlings leaves, and between these enzymes activities and phosphate nutrition negatively related. Fructose-1,6- bisphosphatase and sucrose phosphate synthase activities showed significant difference in second and fourth leaves, but nitrate reductase showed significant difference in first and second leaves in 14days after phosphate starvation treatment. We concluded that phosphate nutrition could affect the distribution of phosphate, carbon and nitrogen in melon seedlings.