• Microbiology and Biotechnology Letters
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• v.22 no.3
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• pp.283-289
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• 1994

The production of maltose utilizing swollen extrusion starch seems to have many technical advantages, such as, high reaction rate and high yield, production of high purity concentrated maltose, and low energy consumption, over the conventional method utilizing liquefied starch. The characteristics of maltose formation in heterogeneous enzyme reaction system comtaining swollen extrusion starch was investigated using fungal $\alpha $-amylase. The influence of extrusion conditions on structure of extruded starch, such as, degree of gelatinization, water absorption index, and water solubility index was analyzed. The relationship between the structural features and maltose forming reaction was investigated, and the result was analyzed in terms of surface reaction of insoluble extruded swollen starch. The characteristics of maltose formation from swollen sxtrusion starch was compared using endo-type fungal $\alpha $-amylase and exo-type $\beta $anylase, and the structural trasformation of extruded starch was also observed to clarify the reaction mechanism.

• Food Science and Biotechnology
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• v.15 no.6
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• pp.986-989
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• 2006

Sweet potato starch noodles prepared without freezing exhibited higher cooking loss and water uptake during cooking and usually resulted in noodles with a softer and stickier texture compared to commercial sweet potato starch noodles manufactured using a freezing process. By utilizing the starches of different plant sources (potato, cowpea, and sago in an equivalent mixture with sweet potato starch), however, the cooking properties and texture of the starch noodles could be improved. Among the starches tested, cowpea starch was most effective in providing cooking and textural properties similar to those of commercial noodles. As an alternative approach, the addition of a minor amount (0.1 % based on total solid weight) of various gums (xanthan, gellan, locust bean gum, curdlan, and carboxymethyl cellulose) was also examined. The addition of curdlan to noodles was effective in increasing the gumminess and hardness, and reducing the stickiness of noodles. Utilizing different starches and gums can improve the overall texture and quality of sweet potato starch noodles produced without freezing.

• Microbiology and Biotechnology Letters
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• v.23 no.4
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• pp.416-424
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• 1995

Mechanism of the cyclodextrin (CD) production reaction by cyclodextrin glucanotransferase (CGTase) using swollen extrusion starch as substrate was investigated emphasizing the structural features of starch granule. The degree of gelatinization was identified to be the most representative structural characteristic of swollen starch. The most suitable degree of gelatinization of swollen starch for CD production was around 63.52%. The structural transformation of starch granule during enzyme reaction was also followed by measuring the changes of the degree of gelatinization, microcrystallinity, and accessible and inaccessible portion to CGTase action of residual swollen starch. The adsorption phenomenon of CGTase to swollen starch was also examined under various conditions. The inhibition mechanism of CGTase by various CDs was identified to be competitive, most severely by a-CD. The mechanism elucidated will be used for development of a kinetic model describes CD production reaction in heterogeneous enzyme reaction system utilizing swollen extrusion starch.

• Korean Journal of Food Science and Technology
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• v.15 no.1
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• pp.46-50
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• 1983

Direct starch-utilizing microorganisms were isolated from 50 samples. Among them, Y-5 strain was selected as one of the potential microorganisms which could utilize starch directly to produce protein or lipid as food resources. The Y-5 strain was identified as a strain of Sporobolomyces holsaticus. It grew on starch or inulin better than on glucose of fructose and its composition was 45% of crude protein, 16% of crude lipid and 9.2% of ash.

• Microbiology and Biotechnology Letters
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• v.22 no.1
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• pp.106-113
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• 1994

A novel method for production of concentrated purity maltose using swollen extruded corn starch was investigated. Degree of gelatinization of extruded starch suitable for maltose formation was found to be around 70%. The optimal amiunt of enzyme was 400 unit fungal $\alpha $-amylase per g of starch, and the reaction time was 12 hours. At extruded starch concentration of 300 g/l(w/v), maltose concentration and content were reached up to 220 g/l(w/v) and 77%(w/w), respectively. The maltose forming reaction was also successfully proceeded at high starch concentration of 700 g/l(w/v), however, the conversion yield and content were decreased. By the addition of extruded starch by fed-batch wise, the maltose concentration, purity, and conversion yield could be improved up to 465 g/l(w/v), 70%(w/w), and 0.63, respectively. The investigated maltose production process seems to have many potential advantages over the conventional process utilizing liquefied starch, and the feasibility for industrial application needs to be evaluated.

• Korean Journal of Food Science and Technology
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• v.27 no.5
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• pp.776-782
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• 1995

The effects of alum$(Al{\cdot}K(SO_4)_2{\cdot}12H_{2}O)$ on the rheological properties of $3{\sim}9%(w/v)$ gelatinized solutions of nonwaxy and waxy rice starches were investigated to evaluate the possibility of utilizing a starch modifying agent. Gelatinized non-waxy and waxy rice starch solutions showed a pseudoplastic flow behavior with yield stress. Pseudoplasticity of two rice starch solutions increased by the addition of alum$(0.05{\sim}1.0%,\;w/w)$. Alum increased the yield stresses and consistency indexes of two rice starches and these values decreased with increasing starch concentration. Also, alum(0.5%, w/w) increased the flow activation energy of gelatinized waxy rice starch solution and activation energy decreased with increasing waxy rice starch concentration in the range of $3{\sim}7%(w/v)$, but for the nonwaxy rice starch, activation energy decreased at starch concentration of 7%(w/v). From the above results, it was found that alum had the possibility of utilizing a modifying agent for the rheological property of gelatinized starch solution.

• KSBB Journal
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• v.7 no.3
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• pp.229-234
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• 1992

Alcohol fermentations were carried out to confirm the capacity of ethanol production from glucose, starch and soluble starch(dextrin) by Schwanniomyces castellii NRRL Y-2477. Schw. castellii NRRL Y-2477 was able to produce the 63.9g/l ethanol using 94% subtrate from 150g/l-glucose medium. The direct alcohol fermentation of starch having the maximum solubility of 20g/1 at $30^{\circ}C$ yielded 9.1g/l ethanol upon complete depletion of starch, whereas 34.5g/1 ethanol was produced by utilizing 82% of 100g/1 soluble starch medium. The fermentation of 150g/1 soluble starch produced 52.1g/l ethanol using about 79% of substrate. Thus, it was found that the limiting step of direct alcohol fermentation of starch by Schwanniomyces castellii NRRL Y-2477 was a hydrolysis of starch.

• Applied Biological Chemistry
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• v.32 no.4
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• pp.408-415
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• 1989

Twenty-seven strains of starch utilizing yeasts were isolated from 30 samples of ‘Nuruk’, a traditional starter in Korea. These strains were identified as ten species belonging to four genera; Hansenula anomaly (six strains), Hansenula sydowiorum (two strains), Saccharomycopsis fibuligera (four strains), Schwanniomyces occidentalis (two strains), Candida fabianii(two strains), Candida famata(one strain), Candida hydrocarbofumarica (three strains), Candida silvicola (one strain), Candida steatolytica (four strains) and Candida tropicalis (two strains). Saccharomycopsis fibuligera Nu-01, Nu-08, Nu-12 and Nu-27 produced much amylase, and one of these, Saccharomycopsis fibuligera Nu-12 showed the highest amylase activity (16.9 IU/ml). Among the isolates, the strains of Hansenula anomala were exhibited relatively high specific growth rate in the medium used starch as a carbon source, and ethanol fermentation by the strains of Candida hydrocarbofumarica was not observed.

• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.158-164
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• 2008

The fusants which contain starch utilizing ability and alcohol fermenting ability were developed by protoplast fusion of Saccharomyces cerevisiae KOY-1 and Saccharomyces diastaticus KCTC 1804. Sacharomyces cerevisiae KH-12 was obtained by haploid induction from Saccharomyces cerevisiae KOY-1. The auxotropic mutants of yeast were obtained by using an ethylmethane sulfonate (EMS). The frequency of protoplast formation in Saccharomyces cerevisiae KOY-1 $(Met^-)$ and Saccharomyces diastaticus KCTC 1804 $(Trp^-)$ were 90.5% and 97.7%, respectively. The frequency of fusant formation was $1.79{\times}10^{-4 }$ for the regenerated protoplast and the 1,000 fusants were obtained. Fusant FA 776 was selected as a potential yeast which contain an alcohol fermenting ability in the starch medium. The genetic stability was 4.64% for 10 passages of generation. Fusant FA 776 produced 13mg/ml of alcohol in 24% starch medium and showed 1.86-fold higher alcohol fermenting ability than Saccharomyces diastaticus KCTC 1804.

• Journal of Microbiology and Biotechnology
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• v.3 no.2
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• pp.95-98
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• 1993

This study was carried out to isolate and characterize the mutant strains of Schwanniomyces castellii NRRL Y-2477. Mutants were prepared with the treatment of ethyl methane sulfonate. 2-deoxy-D-glucose resistant mutants were isolated and two mutants were selected based on their high production of amylolytic enzymes and their ability to ferment starch. The mutants selected had higher a-amylase and glucoamylase activities than the wild type strain from several other carbon sources. Especially, it was revealed that mutant strain M-9, when cultured in the presence of glucose as a sole carbon source, shows relatively high activities of a-amylase and glucoamylase compared to those of the wild type strain. In result, this mutant strain can be considered as a constitutive producer of amylolytic enzymes. To compare the ethanol production ability of wild type strain and of mutant strains selected, an alcohol fermentation was carried out using 100 g/l soluble starch. Mutant strain M-9 did not improve the direct alcohol fermentation of starch, despite its excellent amylolytic activities performance. On the other hand, mutant strain M-6 produced 37.9 g/l (4.8%, v/v) ethanol by utilizing about 82% of substrate.