• Title/Summary/Keyword: Standard Technique

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High Quality Tissue Miniarray Technique Using a Conventional TV/Radio Telescopic Antenna

  • Elkablawy, Mohamed A.;Albasri, Abdulkader M.
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.3
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    • pp.1129-1133
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    • 2015
  • Background: The tissue microarray (TMA) is widely accepted as a fast and cost-effective research tool for in situ tissue analysis in modern pathology. However, the current automated and manual TMA techniques have some drawbacks restricting their productivity. Our study aimed to introduce an improved manual tissue miniarray (TmA) technique that is simple and readily applicable to a broad range of tissue samples. Materials and Methods: In this study, a conventional TV/radio telescopic antenna was used to punch tissue cores manually from donor paraffin embedded tissue blocks which were pre-incubated at $40^{\circ}C$. The cores were manually transferred, organized and attached to a standard block mould, and filled with liquid paraffin to construct TmA blocks without any use of recipient paraffin blocks. Results: By using a conventional TV/radio antenna, it was possible to construct TmA paraffin blocks with variable formats of array size and number ($2-mm{\times}42$, $2.5-mm{\times}30$, $3-mm{\times}24$, $4-mm{\times}20$ and $5-mm{\times}12$ cores). Up to $2-mm{\times}84$ cores could be mounted and stained on a standard microscopic slide by cutting two sections from two different blocks and mounting them beside each other. The technique was simple and caused minimal damage to the donor blocks. H&E and immunostained slides showed well-defined tissue morphology and array configuration. Conclusions: This technique is easy to reproduce, quick, inexpensive and creates uniform blocks with abundant tissues without specialized equipment. It was found to improve the stability of the cores within the paraffin block and facilitated no losses during cutting and immunostaining.

Accuracy Validation of Urinary Flowmetry Technique Based on Pressure Measurement (수압 측정에 기반하는 요류검사의 정확도 검증)

  • Choi, Sung-Soo;Lee, In-Kwang;Kim, Kun-Jin;Kang, Seung-Bum;Park, Kyung-Soon;Lee, Tae-Soo;Cha, Eun-Jong;Kim, Kyung-Ah
    • Journal of Biomedical Engineering Research
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    • v.29 no.3
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    • pp.198-204
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    • 2008
  • Uroflowmetry is a non-invasive clinical test useful for screening benign prostatic hyperplasia(BPH) common in the aged men. The current standard way to obtain the urinary flow rate is to continuously acquire the urine weight signal proportional to volume over time. The present study proposed an alternative technique measuring pressure to overcome noise problems present in the standard weight measuring technique. Experiments were performed to simultaneously acquire both weight and pressure changes during urination of 9 normal men. Noise components were separated from volume signals converted from both weight and pressure signals based on the polynomial signal model. Signal-to-noise ratio was defined as the ratio of the energies between signal and noise components of the measured volume changes, which was 8.5 times larger in the pressure measuring technique, implying that cleaner signal could be obtained, more immune to noisy environments. When four important diagnostic parameters were estimated, excellent correlation coefficients higher than 0.99 were resulted with mean relative errors less than 5%. Therefore, the present pressure measurement seemed valid as an alternative technique for uroflowmetry.

RELATIONSHIP OF IMAGE MAGNIFICATION BETWEEN PERIAPICAL STANDARD FILM AND ORTHOPANTOMOGRAM (표준필름과 Orthopantomograph의 상확대에 관한 비교 연구)

  • Kim Young Tae;Park Tae Won
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.16 no.1
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    • pp.25-30
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    • 1986
  • The author studied the magnification ratio of teeth length in orthopantomogram through intraoral film taken by standardized paral1eing technique. In this study, intraoral radiograph and orthopantomogram were taken in 2 cases of dry skull and 36 adults (504 teeth). 1. The obtained results were as follows: In case of dry skull, the magnification ratio of standard films was 4.6% to 5.9% and that of Orthopantomograph 5 was 15.1% to 33.1%. The magnification ratio of to the standard film was 9.2% to 26.5% 2. In case of adults, the magnification ratio of Orthopantomograph 5 to the standard films was 9.5% to 24.6%. 3. There were no significant difference in magnification between left and right. 4. Anterior teeth had lesser magnification than posterior teeth. 5. It was considered that teeth length showed in Orthopontomograph 5 was magnified 15.4% to 31.3% than actual teeth length.

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On-Site Evaluation Technique of Current Transformer Comparator System (전류변성기 비교측정 장치의 현장 평가기술)

  • Jung, Jae-Kap;Lee, Sang-Hwa;Kwon, Sung-Won;Kang, Jeon-Hong;Kim, Myung-Soo
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.56 no.5
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    • pp.926-932
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    • 2007
  • A recently developed methods for on-site calibration of the current transformer (CT) comparator system have been reviewed in the paper. The method utilizes several traveling standards, which consist of the CT, non-reactive standard resistors, wide ratio error CT, and shunt resistors. The traveling CT is used for absolute evaluation of a standard CT belonging to industry. The non-reactive standard resistors and a wide ratio error CT are used for the linearity check of errors in the current comparator. The shunt resistors are used for evaluation of CT burden of industry.

Study on Comparison of Korean Industrial Standard and ASME BPV Code for Radiographic Examination (방사선투과시험(放射線透過試驗)에 있어서 KS와 ASME Code의 비교(比較)에 관(關)한 연구(硏究))

  • Kim, Jin-Koo;Park, Byung-Chul
    • Journal of the Korean Society for Nondestructive Testing
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    • v.4 no.2
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    • pp.20-29
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    • 1985
  • There are two basic concepts in industrial radiographic examination; one is a radio-graphic sensitivity, and the other is a acceptance criteria. The comparison of these main points are studied for KS Standard and ASME Boiler and Pressure Vessel Code. From the results of the experiment, higher radiographic sensitivity is required in KS Standard when the thickness of material to be examined is less than 20mm in single wall technique. The acceptance criteria for linear type indications are described on same concept in two standards, whereas the acceptance criteria for rounded indications of KS Standard which mainly depends upon the object thickness are more severe than those of ASME BPV Code.

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Development of an Input Impedance Evaluation of the AC-DC Transfer Standard for Low Level AC Voltage Standard (교류 저전압 표준용 교류-직류 변환기의 입력임피던스 평가기술 개발)

  • Kwon, Sung-Won;Jung, Jae-Kap;Lee, Sang-Hwa;Kim, Myung-Soo;Kim, Han-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.2
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    • pp.229-234
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    • 2008
  • An AC-DC transfer standard(TS) is used for the AC voltage standard in the range of 2 mV to 1000 V below 1 MHz. Micro-potentiometer(${\mu}Pot$) is used to evaluate the ac-dc transfer difference(ADD) of the TS below 200 mV range. The ADD of the TS were changed by the loading effect caused from the input impedance change of the TS depend on frequency. An input impedance evaluation technique of the TS using ${\mu}Pot$ has been developed.

Power Standard System for the Calibration and Test of Precision Power Meters (정밀전력계의 교정과 시험을 위한 전력표준시스템)

  • Park, Young-Tae;Ryu, Kwon-Sang;Yu, Kwang-Min;Jang, Seok-Myeong
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.51 no.11
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    • pp.622-627
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    • 2002
  • The power comparison techniques have implemented power measurements, in which a power comparator is used to balance ac against a dc power obtained from known values. The developed power standard system using the comparison techniques consists of dc sources, ac source, control switches, resistive voltage dividers, resistive shunts and a power comparator. The total uncertainty of the power standard system was proved by analysis of the component instruments. Its expanded(k=2) uncertainty is evaluated to be less than 30 uW/VA at unit power factor and 42 uW/VA at power factor 0.5

A Study on the Design criteria of Steel Unit House for the Open System (유닛 모듈라 주택의 설계자동화를 위한 기초 연구(I) - 부품 및 유닛의 DB설정을 중심으로-)

  • Lim, Seok-Ho
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2005.11a
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    • pp.287-290
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    • 2005
  • Precast Concrete apartments were main stream of domestic industrialized housing around 90's , and Steel Houses applying Steel Stud technique with light weighted steel have been dominant portion since 1995. On the other hand, various building techniques including Steel Stud method and highly prefabricated and industrialized Unit method are prevailing in developed countries like Japan. Steel stud and Unit Box have their own merits an demerits, but the more crucial aspect is that the constant design standard should be applied in each design procedure. It entails the necessity of industrial housing development on the Open System basis. In this study, the design standard for Unit house will be established coping with the established preparing standard for design specifications defined by Architectural Law and Promotion Law of Housing Construction. That is for design standard of industrialized private housing on the Open System basis.

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A Study on the Design criteria of Steel Unit House for the Open System (유닛 모듈라 주택의 설계자동화를 위한 기초 연구(II) - 유닛간의 결합/배치/평면구성 원칙을 중심으로-)

  • Lim, Seok-Ho
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2005.11a
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    • pp.291-294
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    • 2005
  • Precast Concrete apartments were main stream of domestic industrialized housing around 90's , and Steel Houses applying Steel Stud technique with light weighted steel have been dominant portion since 1995. On the other hand, various building techniques including Steel Stud method and highly prefabricated and industrialized Unit method are prevailing in developed countries like Japan. Steel Stud and Unit Box have their own merits and demerits, but the more crucial aspect is that the constant design standard should be applied in each design procedure. It entails the necessity of industrial housing development on the Open System basis. In this study, the design standard for Unit house will be established coping with the established preparing standard for design specifications defined by Architectural Law and Promotion Law of Housing Construction. That is for design standard of industrialized private housing on the Open System basis.

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A Study for Robustness of Objective Function and Constraints in Robust Design Optimization

  • Lee Tae-Won
    • Journal of Mechanical Science and Technology
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    • v.20 no.10
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    • pp.1662-1669
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    • 2006
  • Since randomness and uncertainties of design parameters are inherent, the robust design has gained an ever increasing importance in mechanical engineering. The robustness is assessed by the measure of performance variability around mean value, which is called as standard deviation. Hence, constraints in robust optimization problem can be approached as probability constraints in reliability based optimization. Then, the FOSM (first order second moment) method or the AFOSM (advanced first order second moment) method can be used to calculate the mean values and the standard deviations of functions describing constraints and object. Among two methods, AFOSM method has some advantage over FOSM method in evaluation of probability. Nevertheless, it is difficult to obtain the mean value and the standard deviation of objective function using AFOSM method, because it requires that the mean value of function is always positive. This paper presented a special technique to overcome this weakness of AFOSM method. The mean value and the standard deviation of objective function by the proposed method are reliable as shown in examples compared with results by FOSM method.