• Title/Summary/Keyword: Stain test

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Fabrication of Micro Spur Gear in Nano Grained Al Alloy

  • Lee, Won-Sik;Jang, Jin-Man;Ko, Se-Hyun
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09b
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    • pp.778-779
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    • 2006
  • Manufacturing technologies of micro parts were studied in nano grained Al-1.5mass%Mg alloy. During compressive test at $300^{\circ}C$, the Al alloy showed stain softening phenomenon by grain boundary sliding regardless of strain rate. Micro spur gear with ten teeth (height of $200{\mu}m$ and pitch of $250{\mu}m$) was fabricated with sound shape by micro forging. During micro forging, increase of applied stress induced by friction between material and die surface was effectively compensated by decrease of stress by strain softening behavior and as a result, flow stress increased only about 50 MPa more than that in compressive test

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Detection of Theileria sergenti in Bovine Lymphocyte by Polymerase Chain Reaction (PCR 기법을 이용한 소 Lymphocyte 내 Theileria sergenti의 검출)

  • 박진호;이승옥;권오덕;이주묵
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.146-150
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    • 1998
  • 소 림프구내의 Theileria sergenti감염을 확인하기 위하여 T. sergenti감염혈액에서 림프구를 분리한 후 중합효소연쇄반웅을 실시하였다. 또한,분리한 림프구내의 T. sergenti감 염을 증명하기 위하여 IFA test와 acridine orange stain을 실시하였다. 그 결과 다음과 같은 성적을 얻을 수 있었다. T. sergenti 감염헐액의 전혈과 림프구를 각각 생리식염수로 2배율 연 속회석하여 중합효소연쇄반응을 실시한 결과, 림프구내에서는 1,024배 회석배율까지 T. sergenti의 genomic DNA가 중폭되었으며, 전혈내에서는 256배 회석배율까지 증폭되었다. 그리 고 중합효소연쇄반응으로 T. sergenti 감염이 확인된 림프구를 이용하여 IFA test와 acridine orange 염색을 실시한 결과, 림프구내에 T. sergenti가 존재하는 것을 증명할 수있었다. 한편, 중합효소연쇄반응을 이용한 림프구내의 T. sergenti 감염의 진단 유용성을 확인하기 위하여 전 북지역에서 사육중인 소 16두를 대상으로 이들의 혈액으로 PCR 증폭을 실시하였다. 그 결과 전혈에서 genomic DNA를 취한 경우에는 3두(18.8%)만이, 그리고 림프구에서 genomic DNA를 취한 경우에는 11두(68.8%)의 소에서 T. sergenti DNA의 증폭을 관찰할 수 있었다.

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A comparative study on denture cleansers for food stain removal on the denture of elders in domiciliary care (재가복지 노인의치 세정제의 음식물 세정효과 비교연구)

  • Lee, Nam-Soon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.1
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    • pp.331-336
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    • 2018
  • Oral health is an essential requirement for maintaining and promoting health and quality of life while aging. It is known that there is a deep connection with hygienic management of dentures. However, despite the emphasis on the importance of clean management of dentures, there is a lack of quantitative research on denture-related detergents. Therefore, the purpose of this study is to evaluate food stain removal performance of three denture cleansers containing proteolytic enzymes. In addition, this study provides basic data on self-cleaning methods using detergents and suggests ways to improve effective treatment for oral health of elders in domiciliary care. In order to obtain quantitative data concerning food stain removal performance of each proteinase denture cleaner, the test evaluation method proposed by the Korean Consumer Agency, ISO 20795, was used. A total of 18 specimens were discolored for 48 hours in water dissolved with turmeric, and then washed with each denture cleaner for 24 hours. The cleaned denture specimens were measured quantitatively using a spectrophotometer to measure the color values of the denture specimens before the discoloration as well as the color values of the discolored specimens after cleaning with the denture cleanser. The experimental values were analyzed through one-way ANOVA with post-hoc Tukey's test, and statistically significant differences were found among the experimental groups. It was found that C had statistically significant stain removal performance compared with A and B(p<.05). All experiments were conducted for a total of 21 days, from July 3- 23, 2017. This study confirms that denture cleansers is a beneficial self-denture management method for domiciliary seniors. Therefore, it is necessary to extend accessibility for denture cleansers in order to to optimize domiciliary care for elderly oral hygiene and health.

Effects of Hominis Placenta Pharmacopuncture and Electroacupuncture Neuroprotection in Contused Spinal Cord of Rats (척수손상 흰쥐에서 자하거 약침과 침전기 자극이 신경성장인자 발현에 미치는 영향)

  • Kim, Eun-Jung;Kim, Gye-Yeop
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.2
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    • pp.257-263
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    • 2011
  • This study was designed to investigate the effects of Hominis placenta pharmacopuncture treatment and electroacupuncture therapy on the functional recovery and histological change, protein expression in spinal cord injury(SCI) rats. Experimental groups were divided into the Group I(normal control rat), Group II(Non-treatment after spinal cord injury induction), Group III(Hominis placenta pharmacopuncture treatment after SCI induction), Group IV (Electroacupuncture therapy after SCI induction), Group V(Hominis placenta pharmacopuncture treatment and electroacupuncture therapy after SCI induction). After operation, rats were tested at modified Tarlov test at 1 to 3 days with divided into 4 groups, and motor behavior test(BBB locomotor rating scale, Grid walk test) was examined at 3, 7, 14, and 21 days. For the observation of damage change and size of the organized surface in muscle and spinal cord, histopathological studies were performed at 21 days by H & E stain, and BDNF & NT-3 protein expression studies were performed at 21 days. Acco rding to the results, Hominis placenta pharmacopuncture treatment and electroacupuncture therapy can play a role in facilitating recovery of locomotion following spinal cord injury. Specially, Hominis placenta pharmacopuncture treatment and electroacupuncture combimed therapy after SCI induction was most improvement in functional recovery, BDNF, and NT-3 protein synthesis.

Validation of Permanent Deformation Model for Flexible Pavement using Accelerated Pavement Testing (포장가속시험을 이용한 소성변형예측 모델의 검증)

  • Choi, Jeong Hoon;Seo, Youngguk;Suh, Young Chan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.4D
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    • pp.491-497
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    • 2009
  • This paper presents the results of accelerated pavement tests (APT) that simulate permanent deformation (rutting) of asphalt concrete pavements under different temperatures and loading courses. Also, finite element (FE) analysis has been conducted to predict the test results. Test section for APT is the same as one of test sections at Korea Expressway Corporation test road and is subjected to a constant moving dual tire wheel load of APT at three different temperatures: 30, 40, $50^{\circ}C$. The moving wheel is applied at different loading courses within a 75cm wide wheel path to account for traffic wandering. Also, the effect of wandering on permanent deformation development is investigated numerically with three wandering schemes. In this study, ABAQUS is adopted to model APT pavement section with plain stain elements and creep strain rate model is used to take into account viscoplastic stain of asphalt concrete mixtures, and elastic layer properties are back-calculated from FWD measurements. Plus, the effect of boundary condition and subgrade on FE permanent deformation predictions is investigated. A full FE model that accounted for subgrade provided more realistic rut depth predictions, indicating subgrade has contributed to surface rutting.

The Study of Behavior and Histological Change on Treadmill Exercise Intensity after Spinal Cord Injury in Rats (트레드밀 강도에 따른 운동이 척수손상 흰쥐의 행동학적 변화와 조직학적 변화에 관한 연구)

  • Kim, Young-Eok
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.6 no.1
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    • pp.1-15
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    • 2008
  • This study was designed to investigate the effect of treadmill exercise of low-intensity and high-intensity on the functional recovery and histological change in spinal cord injury rats. Sprague-Dawley rats were experimented(n=15) for this research. Spinal cord injury was induced by the NYU drop impacter device after laminectomy. After operation, rats were test at modified Tarlov scale at 3 days, and divided into the control group(n=5), experimental group I(n=5, low-intensity treadmill) and experimental group II(n=5, high-intensity treadmill). The rats were disciplined from 7 day through 21 day. Functional recovery was evaluated by the BBB scales and the Grid Walk test for the progressive locomotor recovery at 3, 7, 14, 21 days. Histopathological studies for the muscle in order to observation the change of damage and size of the organized surface which is visible visually it executed hematoxylin & eosin stain. According to the result of 4 weeks of treadmill exercise, group II showed improvement than group I of motor behavior after spinal cord injury.

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Antibiotic susceptibility of microorganisms isolated from Pneumonic lungs of slaughtered pig in northern Chungnam area (도축돈의 폐렴병소에서 분리한 세균의 항생제 감수성)

  • 이종훈;김성민;배영재;나기복;박일규;정영재
    • Korean Journal of Veterinary Service
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    • v.19 no.2
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    • pp.115-125
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    • 1996
  • A total of 315 microorgainisms were isolated from 256 pneumonic lung samples of pig in abattoirs of northern Chungnam area during February to November 1994, and Identified 97 strains as Pusteurella multocida, 89 strains as Staphylococcus spp, 54 strains as Streptococcus spp, 22 strains as Mycoplasma spp, 21 strains as Escherichia coli, 18 strains as Haemophilus parsuis, 11 strains as Corymebacteroi, pyogenes, and 3 strains as Acrinobacillus spp by Gram's and Dienes stain, and biological properties test Involved API system. After that, they were examined anti biotic susceptibility for ampicillin(AM), cephalothin(CF), chloramphenicol(CP), erythromycin(EM), kanamycin(KM), gentamicin(GM), neomycin(NM), penicillin(PC), streptomycin(SM), tetracycline(TE), tiamulin(TIA), tylosin(TYL), methicillin(DP), colistin(CL) and trimet hoprim(SXT). In antibiotics susceptibility test, 293 isolates except Mycoplasma spp 22 strain were highly susceptible to DF(79.2%) and AM(76.2%), but resistant to PC(14.0%), NM(19.5%) and KM (23.2%) The multiple drug resistant patterns were noted in most isolates, whereas only 7 isolates resistant to single drugs.

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Plant Growth Promotion and Antagonistic Activities Against Anthracnose of Burkholderia sp. LPN-2 Strain

  • Kim, WonChan;Seo, SangHyun;Lee, ChangHee;Park, JunHong;Kang, SangJae
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.3
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    • pp.251-258
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    • 2016
  • A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

Compression D/B for Liquid Segregation Control in Semi-Solid Forming Process and Its Application (반용융 성형공정에 있어서 액상편석제어를 위한 압축 D/B 및 응용)

  • 정경득
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1999.06a
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    • pp.15-32
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    • 1999
  • A relationship between stress and stain is very important to design a die to avoid defects of products during semi-solid forming process. Since the liquid will be of eutectic composition in alloys liquid segregation will result in significant or undesirable situation. The materials used in this experiment are A 357. A390, Al2024 alloys that is fabricated by the electro-magnetic stirring process from Pechiney in France. The compression test was performed by induction heating equipment and MTS. In order to prevent the liquid segregation these measured temperature would be useful to control of strain rate during compression test. The liquid segregation is controlled as change of the strain rate and solid fraction during the compression process, The characteristics of flow between solid and liquid phase considering liquid segregation is examined through the above experiments. In the case of medium and high volume fractions of solid the distribution of strain rate is calculated by using compression test data of semi-solid materials (SSM). The thixoforming experiments with the designed die are carried out successfully. The die filling patterns of SSM for variation of die temperature and pressing force have been investigated. The hardness of the thixoformed scroll products is evaluated in terms of the microstructure for each position.

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Effects of Acanthopanax Senticosus and Onion Mixture Extract on the Collagen-induced Arthritis in Rat Model (가시오가피와 양파 혼합 추출물이 Collagen 유발 관절염에 미치는 영향)

  • Kim, Kyung-Yoon;Sim, Ki-Cheol;Kim, Gye-Yeop;Choi, Chan-Hun;Jung, Jai-Gon;Chung, Jae-Sun;Jeong, Hyun-Woo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.6
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    • pp.1000-1007
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    • 2011
  • This study was conducted to determine the analgesic effect of onion and acanthopanax senticosus mixture extract using the rheumatoid arthritis rat model. Rheumatoid arthritis model was made by the intradermal injection of type II collagen emulsified. Rats were divided into four groups: (1) Sham group(n=5), (2) Control group(administered DW 3 $m{\ell}$/1 day after RA induced, n=7), (3) Experimental group I(administered Onion extractor 600 mg/3 $m{\ell}$/1 day after RA induced, n=7). (4) Experimental group III(administered Onion and Acanthopanax senticosus mixture extractor 600 mg/3 $m{\ell}$/1 day after RA induced, n=7). After that, we examined the arthritic index, paw edema, pain threshold at 1st, 14th, 28th days. And also we examined histopathologic study(safranin-O green), immunohistochamical stain(COX-2) of knee joint at 28th days. Arthritic index, paw edema and pain threshold test were decrease in experimental group I, II than control group. Especially group II was most significantly inhibit effect than the other groups at 28th days. On the histopathologic view, all experimental groups were relieved and reproduced the erosion of arthritic site compared with control group. All experimental groups were COX-2 positive cells in the immunohistological stain of the knee joint were significantly decreased compared with control group. Especially group II was most significantly decreased than the other groups at 28th days. Onion and Acanthopanax senticosus mixture extractor can be used for curing rheumatoid arthritis. Anti-inflammatory effects may be somewhat better in combination of Onion and Acanthopanax senticosus.