• 대한임상검사과학회지
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• 제39권3호
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• pp.223-230
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• 2007

Several methods have been used in the detection of Helicobacter pylori (H. pylori) which was believed to be a pathogenic organism causing chronic gastritis, benign peptic ulcer, gastric carcinoma or malignant lymphoma. Even though several methods were introduced for detection of H. pylori in stomach, there were controversies in their sensitivities and specificities. This experiment were designed to study the comparative analysis of staining methods (hematoxylin and eosin (H&E), Giemsa, Warthin-Starry and immunohistochemical stain) to dectect H. pylori in the gastric mucosa. The results were as follows. Average density score of H. pylori classified by Genta were 2.29 in Warthin-Starry stain, 2.19 in Giemsa stain, 1.34 in immunohistochemical stain and 0.98 in H&E stain. By comparison between inflammatory degree by Sydney system and result of Warthin-Starry stain, the detection rate and densities of H. pylori were increased from mild (61.5% and 0.8), moderate (90.4 and 2.1), and severe (100% and 3.2). From the above findings, Warthin-Starry stain is useful method for detection of H. pylori in gastric mucosa.

• 펄프종이기술
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• 제47권5호
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• pp.33-42
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• 2015

Pitches contained in thermomechanical pulp negatively influence paper qaulity and pulp and papermaking process. Without controlling TMP pitches suitably, it is hard to make a certain quality of paper. In order to control pitches in TMP, they must be quantitatively recognized by proper tools. One of the most widely used way to detecting TMP pitches is a staining method using a hydrophobic dye such as Sudan IV. Sudan IV could be used with three different protocols including different application of its dissolution, washing, dyeing time, etc. The dyeing protocols were classified into three categories including Stain I, Stain II, and Stain III. In dyeing time, Stain I required more than 24 hours to dye pitches. On the other hand, Stain III could stain TMP pitches with the most brief way. The images of red-stained pitches could be captured by a stereomicroscope with ${\times}35$ and ${\times}45$ magnifications, and then quantitatively analysed measuring their numbers and areas by Carl Zeiss AxioVision (ver. 4.8.2) program. Among three protocols, both Stain I and Stain III were the most ideal methods to detect TMP pitches because they detected more pitches and bigger pitch areas compared to Stain II against the same specimen. In particular, it was recognized that Stain III could be used as the most useful tool to detect TMP pitches accurately within several minutes.

• Mycobiology
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• 제42권3호
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• pp.269-273
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• 2014

During 2010 and 2012 grape harvest seasons in Gyeonggi-do, Korea, a white stain symptom was observed on the harvested grape fruits in 'Campbell-Early' and 'Kyoho' varieties. In samples collected from the infected vine, two different strains of pathogenic fungi have been found and identified as Acremonium acutatum and Trichothecium roseum based on fungal morphology and nucleotide sequence of internal transcribed spacer (ITS) and supported by the phylogenetic analysis of the rDNA-ITS region. The DNA homologies of the isolated strains were 99.8% and 99.6% identical with T. roseum (IFB-22133) and A. acutatum (CBS682.71), respectively. In the pathogenicity test, the spores of A. acutatum and T. roseum sprayed on the grapes caused white stain symptoms on the fruits in two weeks after the artificial inoculation, which is similar to observations in the field. To our knowledge, this is the first report of white stain symptoms caused by A. acutatum and T. roseum on the grapes in Korea.

• 한국세라믹학회지
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• 제35권4호
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• pp.378-384
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• 1998

We investigated the possibility of systemizing data for the colour gradation of classical ceramics by build-ing program and D-basing each data information in pottery field that has been fall behind in forming data be-cause of many variable and lacking of data system. In this study we manufactured $CoAl_2O_4$ stain that has stable spinel structure at high temperature and then mixed base glaze with stain for the additive per-centage of 0.5 , 1, 5, 10, 15 wt% In thie colouring of Lime base glaze with the wide ranged melting tem-perature the colour development of cobalt-blue sintered at $1250^{\circ}C$ is better than that of $1150^{\circ}C$ $1350^{\circ}C$ The experimental conditions and data are D-based simulated programming of computer. The result of -based data appeared to be more smoothed fusion-adherence in the Matt glaze range of Lime base glaze, The variation of colouring is not found when additive content of glaze is more than 5 wt% and the stable colour development of stain was shown at the content that RO, $R_2O$ percentage is high (the content that R2O3 per-centage is low)

• The Journal of Advanced Prosthodontics
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• 제9권2호
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• pp.138-142
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• 2017

In restorative treatment using fixed dental prostheses, dentists should select appropriate restoration material among various types of dental materials. The strength, marginal fit, esthetics, wear resistance, biocompatibility, and cost are important factors in the choice of restoration materials. The present case showed a surface stain on a monolithic zirconia restoration that was due to wear between the monolithic zirconia restoration and the base metal alloy restoration. This phenomenon was confirmed by surface roughness measurement and electron probe micro-analysis.

• 한국세라믹학회지
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• 제23권4호
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• pp.23-26
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• 1986

The synthetic conditions of $ ZrSiO_4-Cd(S_xS_{1-x})$ stains from CdS, Se, $SiO_2$ , 4ZrO_2$ and Lif were investigated and the colors were examined. Colors of the stains prepared were yellow orange red pink ruby and violet in relation to both the content of CdS-Se in $ZrSiO_4$ and firing temperature. Colors of these series stains were thermally stabilized probably by the structural stability of zirconium silicate. Furthermore by the result of X-ray diffraction analysis it is assumed that color of the zircon cadmium sulphoselenide $ ZrSiO_4-Cd(S_xS_{1-x})$ stain is developed by neither the coloring ions in $ZrSiO_4$ lattice nor the solid solution of $ZrSiO_4$ and $Cd(S_xS_{1-x})$ but by the small crystals of $Cd(S_xS_{1-x})$ being occluded by the zirconium silicate during sintering process.

• Journal of the Optical Society of Korea
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• 제7권4호
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• pp.234-239
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• 2003

Objective methods to assess quantitatively port wine stain (PWS) blanching in response to laser therapy are needed to improve laser therapeutic outcome. Previous studies have attempted to assess objectively PWS color based on point measurement devices. To date, these approaches have typically been limited by a number of factors such as small test area and need for contact. To address these issues, a polarization spectral imaging system and an image analysis method have been developed to evaluate quantitatively erythema and melanin content distribution in skin. The developed polarization spectral imaging system minimizes artifacts such as glaring, shadowing, and non-uniform illumination that interfere with image fidelity. Furthermore, the image analysis method has been employed to get images of skin melanin and erythema indices from the acquired color images for quantitative analysis. Finally, using PWS patient color image, the effectiveness in laser treatment of PWS was evaluated by calculating relative erythema index image that is the relative erythema index of PWS region to the normal region. The developed device and analysis method appears to be a simple and effective method for quantitative analysis of PWS blanching.

• 한방재활의학과학회지
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• 제19권1호
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• pp.187-199
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• 2009

Objectives : The electroacupuncture was generally has been used in oriental medicine doctors. In recent years, there have been a few studies about safety and stability of acupuncture needle in itself, but then again research of acupuncture needle safety and stability on applying electroacupuncture have been insignificant. Therefore we investigated the safety and change in mechanical characteristic of acupuncture needle in electroacupuncture therapy. Methods : We observed mechanical characteristic change by SEM(Scanning Electron Microscope) and EDX(Energy Dispersive X-ray Spectroscopy), evaluated the hardness by vickers hardness tester. We used MTT assay and cell stain to study about biocompatibility of electroacupuncture. Results : In this study, any corrosion of material, alternation of elements, and change of hardness were not observed in surface analysis using SEM and EDX. In cytotoxity evaluation using MTT assay and cell stain, cell survival rate was low when practicing the electroacupuncture for more than 3 hours. Conclusions : Change of mechanical property was not observed based on the test results using surface analysis and hardness estimation by the electroacupuncture. And considering the biocompatibility, electroacupuncture was thought to be safe in an hour based on cytotoxity evaluation using MTT assay and cell stain.

• 대한치과보철학회지
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• 제35권2호
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• pp.344-356
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• 1997

The purpose of this study was to evaluate the long term tooth-brushing effect on the color change of specially treated IPS Empress porcelain surface. Staining techique with blue stain and liquid was used. The surfaces of the specimen were treated with 5% and 10% Hydrofluoric acid, 50mm and 250mm alumina sandblast, and then blue stain and liquid were used for external stain. After 29,200, 58,400, 87,600, 116,200 brushing strokes (equivalent to 2, 4, 6, 8 years each), color changes of the stained layer were measured with spectrophotometer(CM-3500d, MINOLTA, Tolkyo, Japan). The result of this study was obtained as follows : 1. The color changes were great after 29,200 tooth brushing strokes in every group, but from 29,200 strokes to 116,800 strokes, there were no significant color changes in each group. (p>0.05) 2. The greastest color changes were observed in 5% HF treated group and the least color changes were observed in $50{\mu}m$ aluminar sandblast treated group in every stroke (p<0.05), but no statistical difference between groups compared with control group (p>0.05). According to these results, pretreatment of I.P.S. Empress porcelain with alumina sandblast improved the color stability It is thought that about 8 year-tooth brushing strokes does not change the color of IPS Empress porcelain significantly on this condition, but long term follow-up will be needed.

• 보존과학회지
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• 제36권6호
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• pp.505-510
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• 2020

본 연구에서는 전통적인 방법으로 재현한 전통 명유, 전통 명유를 과학적으로 분석하여 친환경적인 방법으로 개발한 크린 명유, 그리고 명유의 원료이자 현재 목조문화재를 보수할 때 단청의 마감제로 사용되고 있는 생들기름의 방미효력을 평가하였다. 평가 결과, 생들기름은 방미효력이 거의 없는 것으로 나타난 반면에, 두 종류의 명유는 모두 높은 성능의 방미효력을 보였다. 하지만, 명유를 천연용제인 테르펜유에 희석하여 사용하면 균주에 노출된 지 4주 만에 방미효력이 현저히 감소하는 것을 확인 할 수 있었다. 목재에 처리된 약제의 함량이 명유의 방미효력에 영향을 주는 것으로 판단된다. 즉, 목조건축물을 축조함에 있어서 명유가 들기름보다 곰팡이 발생을 억제하기 위한 마감제로 더 적합하며, 야외 환경에서의 곰팡이 성장 억제를 저해 할 수 있는 최적의 처리량 및 처리 방법에 대한 추가 연구를 통하여 명유의 적용 가능성을 높힐 수 있을 것으로 생각한다.