• Tuberculosis and Respiratory Diseases
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• 제72권2호
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• pp.124-131
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• 2012

Background: DNA damage-inducible 1 (Ddi1), one of the ubiquitin-like and ubiquitin-associated family of proteins, may function in the regulation of the ubiquitin-proteasome pathway, which has been validated as a target for antineoplastic therapy. We investigated Ddi1 expression in human lung cancer tissues and evaluated the relationship of this expression pattern with clinicopathological factors in patients with non-small-cell lung cancer (NSCLC). Methods: Ddi1 expression was examined by immunohistochemistry in tumor tissues from 97 patients with stage I NSCLC, who had undergone curative surgical resection at two tertiary referral hospitals from 1993~2004. None of the patients received preoperative chemotherapy and/or radiation therapy. Results: Thirty-nine (40.2%) of the 97 cases were positive for Ddi1. Ddi1 expression was dominantly seen in cytoplasm rather than in the nuclei of cancer cells in all histological types, whereas adjacent nontumoral lung tissue showed negative Ddi1 staining in most cases. Ddi1 expression tended to increase in well-differentiated tumors but without statistical significance. Positive Ddi1 expression was associated with a tendency for better disease-free survival and disease-specific survival, although the difference was not significant. Conclusion: Ddi1 expression is a property of NSCLC. Because Ddi1 could be a potential target for cancer therapy, more research is needed to evaluate its role in NSCLC.

• 생명과학회지
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• 제16권4호
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• pp.704-709
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• 2006

본 연구는 DNA 상해유도기작을 규명하기 위하여 꼬마선충 (Caenorhabditis elegans) 으로 부터 subtraction hybridization 방법을 이용하여 자외선 유도 유전자인 UV100과 UV150을 분리하고 그 유전자 구조와 발현양상을 조사하였다. 분리한 유전자의 발현양상을 Northern hybridization 방법으로 살펴본 결과 자외선 조사 후에 최대 2배 이상의 발현 증가를 나타내었다. 이 결과 이미 밝혀진 다른 UV-inducible 유전자와 유사하게 UV100과 UV150 유전자는 자외선에 의해서만 발현이 증가됨을 알 수 있었다. 또한 분리한 유전자의 기능을 알기 위하여 RNAi 실험을 한 결과 분리한 자외선 유도유전자는 발생단계에 따라 다양한 DNA 회복기작을 나타냄을 알 수 있었다.

• BMB Reports
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• 제55권10호
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• pp.512-517
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• 2022

Traumatic brain injury (TBI) is brain damage which is caused by the impact of external mechanical forces. TBI can lead to the temporary or permanent impairment of physical and cognitive abilities, resulting in abnormal behavior. We recently observed that a single session of early exercise in animals with TBI improved their behavioral performance in the absence of other cognitive abnormalities. In the present study, we investigated the therapeutic effects of continuous exercise during the early stages of TBI in rats. We found that continuous low-intensity exercise in early-stage improves the locomotion recovery in the TBI of animal models; however, it does not significantly enhance short-term memory capabilities. Moreover, continuous early exercise not only reduces the protein expression of cerebral damage-related markers, such as Glial Fibrillary Acid Protein (GFAP), Neuron-Specific Enolase (NSE), S100β, Protein Gene Products 9.5 (PGP9.5), and Heat Shock Protein 70 (HSP70), but it also decreases the expression of apoptosis-related protein BAX and cleaved caspase 3. Furthermore, exercise training in animals with TBI decreases the microglia activation and the expression of inflammatory cytokines in the serum, such as CCL20, IL-13, IL-1α, and IL-1β. These findings thus demonstrate that early exercise therapy for TBI may be an effective strategy in improving physiological function, and that serum protein levels are useful biomarkers for the predicition of the effectiveness of early exercise therapy.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.55-55
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• 2023

With the COVID-19 pandemic, there is increasing interest in anti-obesity strategies. According to the National Statistical Office, the obesity rate in Korea was 38.3% in 2020 and 37.1% in 2021. Obesity is a risk factor for several severe diseases, including stroke, heart disease, type 2 diabetes, and certain types of cancer. Pinus rigida × Pinus taeda is a hybrid of Pinus rigida Mill and Pinus taeda Linn, and its cones are considered a by-product. Although previous studies have investigated their pharmacological effects on antioxidant activity and protection against oxidative DNA damage, few researchers have explored their potential as functional natural materials. Therefore, we evaluated the anti-obesity effects of the cone of ethyl acetate fraction of P. rigida × P. taeda (ERT), specifically its ability to inhibit lipid accumulation. Our analysis showed that ERT contains phytochemicals (catechin and caffeic acid) which are known to improve immune function and inhibit cell damage. ERT inhibited lipid droplet accumulation at the cellular levels through Oil Red O staining. Furthermore, ERT suppressed the expression of adipogenic transcription factors (PPARγ and CEBP/α) as well as downstream lipogenic target genes (FAS and SREBP-1) thereby inhibiting adipogenesis. ERT also down-regulated key adipogenic markers, including aP2α, while inducing the phosphorylation of AMPK. It has been reported that PPARγ and CEBP/α are expressed in the early stages of adipose differentiation, while SREBP-1 is expressed in the late stage. Therefore, our findings suggest that ERT activates AMPK signaling pathways, which inhibits adipogenic transcription factors (PPARγ, C/EBPα, and SREBP1) and lipogenic genes (FAS and aP2α), thereby blocking lipid accumulation and preventing obesity and related disorders. ERT showed potential as a new resource for developing a functional material for anti-obesity agents.

• Annals of Clinical Neurophysiology
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• 제4권2호
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• pp.137-139
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• 2002

Background : Subtle status epilepticus (SE) is an end-stage of convulsive SE. This phenomenon might be a clinical expression of neuronal exhaustion caused by sustained electrical discharges. As subtle SE may show diverse clinical features, early detection depends on clinical suspicion. Case : A 68-year-old woman was presented with repetitive involuntary movement of right limbs after two generalized tonic-clonic seizures. She experienced right middle cerebral artery infarction 4 months ago, and after the event, left side hemiplegia sustained. These seizures were first-ever after the cerebral infarction. Orientation and verbal responses were fairly preserved but general cognitive function was minimally slowed. During the video-EEG monitoring, repetitive sharp waves were noted in the right hemisphere and these sharp waves occasionally spread to the contralateral side. Her right side involuntary movement was identifiable when the epileptic discharges were found on her right hemisphere. Conclusion : We suggested that this unexpected convulsive movement is a reflection of earlier exhaustion in the right hemisphere or deefferentation of right hemisphere because of preexisting neuronal damage.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2006년도 학술발표회 논문집
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• pp.770-774
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• 2006

The determination of feasible design flood is the most important to control flood damage in river management. Model parameters should be calibrated using observed discharge but due to deficiency of observed data the parameters have been adopted by engineer's empirical sense. Storage coefficient in the Clark unit hydrograph method mainly affects magnitude of peak flood. This study is to estimate the storage coefficients based on the observed rainfall-runoff events at the four stage stations in the Hantan river basin. Model calibration is the process of adjusting model parameter values until model results match historical data. An objective function which is the percent difference between the observed and computed peak flows is available for measuring the goodness-of-fit between computed and observed hydrographs. By sensitivity analysis for the storage coefficient, it has been shown that the storage coefficients affect the peak flows. The Clark parameters adopted in the River Rectification Basic Plan have been estimated through an iterative process designed to produce a hydrograph with the peak flow.

• 한국정밀공학회지
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• 제28권11호
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• pp.1309-1315
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• 2011

The design process for obtaining the reliable steeraxle casting beam of fork lift truck is studied in this paper, as the casting beam is major component of steeraxle which has a steering function at driving. In this study, the driving mode and damage pattern of casting beam which could be occurred from the customer site were analyzed and it established the design process to predict the fatigue life by FEA(Finite Element Analysis) so that the reliability of steeraxle casting beam could be verified at DVT(Design Validation Test) mode. This paper provides guidance on the process of designing the reliable steeraxle casting beam at the initial design stage and also, provides guidance on the process of solving the problem when the failure is occurred in the field.

• 한국습지학회지
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• 제10권3호
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• pp.69-78
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• 2008

최근에 들어서 홍수조절과 생태적 기능을 모두 만족시키는 방안으로서 천변저류지에 대한 관심이 증가하고 있다. 본 연구에서는 경상남도 창녕군의 토평천 유역을 대상으로 하여 천변저류지를 설치하였을 경우 천변저류지로 인한 홍수조절 효과를 분석, 검토하였다. 천변저류지는 우포늪의 상류 및 중하류에 골고루 분포하는 것을 가정하였으며, 홍수위 저감 효과 이외에도 침수 면적과 피해 저감 등의 홍수 조절 효과를 분석하였다. 연구 결과 천변저류지로 인하여 홍수 조절 효과가 있는 것으로 나타났으나, 여러 천변저류지의 형식이나 조합에 따라서 조절 효과는 상이하게 나타났다. 따라서, 천변저류지를 설치할 경우 저류용량의 극대화 보다는 실제적인 홍수 조절효과 분석을 통한 홍수 조절 효과의 극대화를 지향하는 것이 타당하다고 보여진다.

• Nuclear Engineering and Technology
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• 제48권1호
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• pp.218-227
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• 2016

Background: Steam explosions may occur in nuclear power plants by molten fuel-coolant interactions when the external reactor vessel cooling strategy fails. Since this phenomenon can threaten structural barriers as well as major components, extensive integrity assessment research is necessary to ensure their safety. Method: In this study, the influence of yield criteria was investigated to predict the failure of a reactor cavity under a typical postulated condition through detailed parametric finite element analyses. Further analyses using a geometrically simplified equivalent model with homogeneous concrete properties were also performed to examine its effectiveness as an alternative to the detailed reinforcement concrete model. Results: By comparing finite element analysis results such as cracking, crushing, stresses, and displacements, the Willam-Warnke model was derived for practical use, and failure criteria applicable to the reactor cavity under the severe accident condition were discussed. Conclusion: It was proved that the reactor cavity sustained its intended function as a barrier to avoid release of radioactive materials, irrespective of the different yield criteria that were adopted. In addition, from a conservative viewpoint, it seems possible to employ the simplified equivalent model to determine the damage extent and weakest points during the preliminary evaluation stage.

• International Journal of Safety
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• 제5권2호
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• pp.18-23
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• 2006

Studies have claimed that ESE (Early Streamer Emission) air terminals offer a vastly increased zone of protection over that of traditional lightning rods (Franklin rods) by causing the emission of an upward streamer/leader: The upward streamer/leader will propagate towards the tip of the downward leader at an early stage in the attachment process.. This paper shows the results of a performance evaluation test of a particular type of the ESE air terminal (called "ElecHippo") with a simple rod tested at the Korea Electrotechnology Research Institute (KERI). The corona emission current of the ElecHippo made by Yong-Jin Enterprise Corp. has also been measured at the Occupational Safety & Health Research Institute (OSHRI). The results show that the ElecHippo meets the French standard of NFC 17-102-1995. The results also verify the ESE performance by measuring the ion emission current generated in the discharge electrode gap as a function of the capacitance and inductance of the equipped devices. Finally, we propose a new method for grounding the system to reduce the lightning damage by combining the ESE air terminal, the early discharge earth plate, the lightning strike recorder and the surge protection device.