• Asian-Australasian Journal of Animal Sciences
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• v.14 no.9
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• pp.1272-1275
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• 2001

Two hundred and forty post-larvae (PL) of fresh water prawn, Macrobrachium rosenbergii were distributed in eight treatment groups with three replicates each. Eight experimental diets were prepared by using squilla (Orato squilla nepa), squid (Sepia pharonis) and clam (katelysia opima) alone or in different combination as source of animal protein and compared to a control diet containing fish meal. Total crude protein content for all dietary treatments was around 32%. Total protein content of fish meal was replaced by an equal amount of protein from different animal protein sources on isonitrogenous basis. Diets were fed at 5% of the body weight of post-larvae twice daily. The experiment was conducted for a period of 60 days. It was found that all the above three protein sources could be used by completely replacing fishmeal except clam meal at higher level of inclusion (26%). A combination of squid and squilla meal at 14% each in the diet increased the growth performance of PL significantly (p<0.05) in terms of Specific growth rate (SGR) % (5.17), FCR (2.12) and PER (1.51). Squilla meal can be used to the maximum level of 38% without any growth depression.

• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.3
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• pp.307-312
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• 1984

The dried pure shells deprived of soft tissues were subjected to analysis of chitin-protein complexes from 5 species of marine crustaceans, including 2 species of crabs and 3 species of shrimps. The protein fractions were obtained from chitin-protein complexes under the varying conditions of extractions and the crude chitin was prepared from the shells by the sulfurous acid process. The crude chitin was purified through the extraction with several organic solvents such as dimethyl-acetamide, N-methylpyrrolidone. The purified chitin was also examined using the phase contrast microscope. Total protein contents of the shells were diverse, showing 9.6% for Portunus trituberculatus, 3.1%, Charybdis bimaculata, 9.4%, Penaeus japonicus, 10.9%, Metapenaeus intermedius and 5.8%, Squilla oratoria. Covalently bound protein varied with species from 2.1% for Charybdis bimaculata to 9.9% for Metapenaeus intermedius. The puified chitin contents of the shells were shown to 21.1% for Portunus tritube rculatus, 6.2%, Charybdis bimaculata, 20.2%, Penaeus japonicus, 27.1%, Metapenaeus intermedius and 25.5%, Squilla oratoria. Exceptionally low analytical value obtained with Charybdis bimaculata are supposed to be due to the very young subjects. The ratios of chitin to covalently bound protein in the shells were various such as 2.7 to 1 for Portunus trituberculatus, Penaeus japonicus and Metapenaeus intermedius, 3.1 to 1, Charybdis bimaculata and 6.1 to 1, Squilla oratoria. The microscope finding of the purified chitin showed the filamentous form in all the specimen.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.408-420
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• 2016

This study proposes an alternative approach for the use of chitosan silver-based dressing for the control of foot infection with multidrug-resistant bacteria. Sixty-five bacterial isolates were isolated from 40 diabetic patients. Staphylococcus aureus (37%) and Pseudomonas aeruginosa (18.5%) were the predominant isolates in the ulcer samples. Ten antibiotics were in vitro tested against diabetic foot clinical bacterial isolates. The most resistant S. aureus and P. aeruginosa isolates were then selected for further study. Three chitosan sources were tested individually for chelating silver nanoparticles. Squilla chitosan silver nanoparticles (Sq. Cs-Ag⁰) showed the maximum activity against the resistant bacteria when mixed with amikacin that showed the maximum synergetic index. This, in turn, resulted in the reduction of the amikacin MIC value by 95%. For evaluation of the effectiveness of the prepared dressing using Artemia salina as the toxicity biomarker, the LC₅₀ was found to be 549.5, 18,000, and 10,000 μg/ml for amikacin, Sq. Cs-Ag⁰, and dressing matrix, respectively. Loading the formula onto chitosan hydrogel dressing showed promising antibacterial activities, with responsive healing properties for the wounds in normal rats of those diabetic rats (polymicrobial infection). It is quite interesting to note that no emergence of any side effect on either kidney or liver biomedical functions was noticed.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.4
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• pp.248-262
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• 2005

The purpose of this study was to identify the state of demersal fish resource catch by small trawlers, which live in the southern waters off Goheung. We investigated the results of catch of sample fishing vessels, and performed fishing experiments using the actual fishing operation vessels from early November in 2002 till end of October in 2003. The daily amount of catch per vessel of the 35 small trawlers selected as sample vessels was the highest in summer seasons(June and July) as 70kg and the lowest in winter seasons(January and February) as 45kg and Octopus minor occupied as 17 to 30kg nearly 30% of the total catch. Additionally the catch of Octopus minor per vessel, per dragging hour ranges 3 to 6kg, which is the highest in March and June and the low in January to February, April to May and September. In the fishing experiments using small trawler, during the study period, a total of 75 fish species were collected. The number of individuals by species consisted 58.2% in Shrimps, 17.8% in Fish, 2.3% in Cephalopod. Of these, Parapenaeopsis tenella was the highest in 29.2%, Squilla oratoria and Crangon hakodatel was 14.6% respectively and Octopus minor was 0.2% of the total number of individuals. As far as the appearance number of individuals by month was concerned, February was the highest and then May, April and June followed in order, and October showed the lowest. Additionally the monthly catch per dragging was the lowest in December to January as 20kg and the highest in July as 160kg. Specially, Octopus minor was caught throughout the year regardless of season and the catch was the highest at the period from March to June. When looking into the body mean length of dominant fishes caught, we could observe the followings; Trachurus japonicus 8.9cm, Cynoglossus robustus 10.8cm, Muraenesox cinereus 15.3cm, Setipinna taty 10.3cm, Amblchaeturichfhys hexanema 9.3 cm and Collichthys niveatus 8.9cm, most of which were in their immaturity when they were caught.