• Journal of Aquaculture
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• v.16 no.2
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• pp.104-109
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• 2003

The present study aims to evaluate protein and phosphorus availabilities of five different dietary protein sources during the 6-week feeding trial in juvenile olive flounder, Paralichthys olivaceus as determined by growth performance and phosphorus retention. Five diets containing blood meal (BM), poultry by-product (PBP), squid liver powder (SLP), feather meal (FM) and soybean meal (SM) were prepared by mixing a basal diet (BD) with one of five test ingredients at the ratio of 7 to 3. As a reference diet, BD contains three different protein sources such as white fish meal, casein and gelatin. After 2 weeks of the conditioning period, fish initially averaging 2.7$\pm$0.02g (mean$\pm$SD) were randomly distributed into each aquarium as a group of 30 fish reared in the recirculating system. Fish of triplicate groups were fed one of six experimental diets (BD+five test diet). After 6-week feeding trial, pro- tein efficency ratio (PER) of fish fed BM diet was the lowest in experiment groups. While fish fed PBP diet showed a significant higher PER as compared to the FM diet, and fish fed SLP diet and BD were a higher PER than did fish fed PBP diet. However, there was no significant difference in PER among fish fed SLP diet, BD and SM diet, and between SM diet and PBP diet. Phosphorus retention efficiency of bone (PR $E_{b}$) of fish fed BM diet was the lowest in all the diets, and fish fed FM diet showed a higher PE $R_{b}$ than fish fed BD and SM diet. However, there was no significant difference in PER among fish fed FM diet, SLP diet and PBP diet, and among SLP diet, PBP diet, SM diet and BD. These results indicate that SLP could be a suitable protein source for low pollution diets of olive flounder in the future fish feeds market. Furthermore, PBP and SM are available protein source to reduce P waste in the oliver flounder aquaculture with the use of proper mixture of other protein sources and more processing to improve protein availibility of these.ese.

• Journal of Aquaculture
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• v.10 no.2
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• pp.143-151
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• 1997

A six week feeding trial was conducted to determine the amount of fish meal analog (FMA) that can be replacing fish meal protein (FM) in Korean Rockfish. Seven experimental diets were formulated on isonitrogenous 52% crude protein and isocaloric basic 16.8KJ/g diet. Also, foreign commercial fish meal analog (CFMA) and attractants (ATT) were tested in this experiment. Percentage of the graded level of replacement of FM by FMA/CFMA on the basis of crude protein were as following : Diet 1, 100%FM ; Diet by 2, 60%FM : 40% CFMA ; Diet 3, 60%FM : 40$ CFMA+ATT ; Diet 4, 80%FM : 20%FMA ; Diet 5, 80%FM : 20% FMA+ATT ; Diet 6, 60%FM : 40%FMA+ATT ; Diet 7, 40%FM : 60% FMA+ATT. The FMA was made by mixing six animal protein source such as th blood meal, squid liver powder, meat and bond meal, leather meal, feather meal, poultry by-product and 3 essential amino acids (Met, Lys, Ile). Weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed diets 4, 5 and 6 were not significantly different (P>0.05) from those of fish fed the control (100% FM), while those of fish fed diets 2 and 3 were significantly lower (P<0.05) than those of fish fed the diet 6. There was no significant ATT effects in this study (P>0.05). Significant differences were found in hepatosomatic index, hemoglobin and condition factor. Therefore, these, results indicated that FMA can be used up to 40% as a substitute of fish meal protein in Korean Rockfish diets.