• Journal of Environmental Health Sciences
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• v.24 no.1
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• pp.141-150
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• 1998

This study was performed to investigate the distribution of Yersiniae and correlation between Yersiniae and indicator organism by time and area in spring water located in northern part of Seoul. Samples collected from 46 spring waters located in four mountains(Dobong, Bukhan, Surak, Bulam) were inspected to detect Yersiniae and indicator organisms. And also there were examined bioserological characteristics and resistance of ahtibiotics of the isolated Yersiniae.The result were as follows. 1. The isolation rate of Yersiniae was 22% in February and 20% in April. The isolated species were 6 strains of Y. enterocolitica, 6 strains of Y. aldova, 4 strains of Y. intermedia and 43 strains of Y. pseudotuberculosis. The serotype of Y. pseudotuberculosis isolated from was all O:5 and biotype of Y. enterocolitica isolated from was all O:3. 2. The Geometric mean of standard plate count, coliform, and psychrotrophilic bacteria were 3.4 CFU/ml, 1.2 MPN/100 ml and 33.0 CFU/ml in February and 3.1 CFU/ml, 1.5 MPN/100 ml and 20.5 CFU/ml in April respectively. There was no significant difference by time and area but the indicator organisms were correlated significantly with each other (p<0.05). 3. Because detection of Yersiniae was not statistically associated with indicator organism, Yersiniae can be detected in the spring water approved microbiologically (p<0.05). 4. The Yersiniae isolated were resistant to Ampicillin, Colistin, Carbenicillin and Coilstin. All isolaed Y. enterocolitica were resistant to Ampicillin (100%). In the case of Y. pseudotuberculosis, only 1 of 3 isolated was resistant to Colistin but susceptible to other antibiotics.

• Journal of Food Hygiene and Safety
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• v.13 no.3
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• pp.305-312
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• 1998

In order to investigate the species, serobiological characteristics and antibiotic sensitivity of Pseudomonas spp, we isolated Pseudomonas spp from 57 spring waters around Seoul area for spring, summer and autumn and identified Pseudomonas spp by biochemical characteristics and serological method. And also we tested the antibiotic sensitivity test by discdiffusion method. Of 57 spring waters tested, Pseudomonas spp were isolated from 33 spring waters(57.9%). Isolation rate of Pseudomonas spp in spring season was 28.1%, summer 21.1% and autumn 28.1%. Only 1 spring water was detected Pseudomonas spp in all seasons and 9 (15.8%) were detected for 2 seasons and 13 (22.8%) were for only 1 season. Isolation rate of Pseudomonas spp at Mt. Cheonggye was 50% and followed by Mt. Bookhan 35.7%, Mt. Daemo 33.3%, Mt. Dobong 29.6%, Mt. Surak 25.9%, Mt. Woomyun 22.2% and Mt. Bulam 7.4%. Of 44 Pseudomonas spp, 22 strains (50%) were identified by Ps. putida, Ps. aeruginosa, Ps. fluorescens and Ps. mendocina were identified 6 strains (13.6%), respectively. 4 strains (9.1%) were identified by Ps. aureofaciens. Of 6 Ps. aeruginosa, serotype A was 2 strains, B, E, G, and K was 1 strain, respectively. Of 44 Pseudomonas spp, resistance rate to amoxicillin was 90.9% and followed by chloramphenicol 84.1%, tetracycline 84.1%, carbenicillin 81.8%, nalidixic acid 68.2%, neomycin 38.6%, streptomycin 31.8%, gentamicin 4.6%, kanamycin 4.6% and colistin 2.3%. Ps. aeruginosa was more sensitive to carbenicillin than other Pseudomonas spp isolated from spring waters in Seoul area but more resistant to kanamycin, and Ps. aureofaciens was no resistant to streptomycin. Among multiple drug resistance, resistance to 5 drugs was 31.8%, 4 drugs 15.9%, 7 drugs 13.6%, 1 drug and 2 drugs 4.6%, and 8 drugs 2.3%, respectively. The multiple resistance patterns detected highestly were NA-CB-C-TE-AMC (18.2%), NA-CB-N-C-TE-AMC (13.6%), CBC-TE-AMC (11.4%) and NA-CB-N-C-TE-AMC-S (9.1%).