• The Korean Journal of Pesticide Science
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• v.17 no.1
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• pp.60-64
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• 2013

This study was performed in order to assess the risk of pesticide operator in korean rice paddy. The amount of exposure was calculated based on the informations of 48 kind of items that are used the form of a spraying in korean rice paddy. The risks of these pesticides were assessed to compare the exposure dosage with AOEL (acceptable operator exposure level) which was released form developed countries. When the operator spraying pesticides using motor sprayer, 17 items showed the risk for operator if the operator have not worn the personal protective equipment (PPE), and 6 items showed the risk for operator if the operator have worn the PPE. These result were indicated that the risk of pesticide operator was very high in korean rice paddy.

• Journal of Biosystems Engineering
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• v.10 no.1
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• pp.13-23
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• 1985

Spraying method from the paddy-field levee is known to give insufficient penetration of the spray droplets to the rice stem of the densely grown plants, which is generally encountered at the last stage of rice growth. This study was intended to investigate the spraying system to solve this existing problem. As an approach, it was attempted to develop the boom-with-nozzle, between-the-row application system. Several types of nozzles and their different arrangements in the boom were tested in the field to measure the penetration-reaching distance and the uniformity of spray droplet distribution. The results of the study are summarized as follows: 1. Field experiments by the spraying method from the paddy-field levee showed to have practically no penetration of the spray droplets to the portion of the plant stem with the normal flow volume generally applied and thus need for improving present spraying method. 2. It was found that, considering both the uniformity of the spray droplet distribution and performance rate, the most efficient type of nozzle in the between-the-row boom-type spraying system was one that has core-insert hollow-cone with some clearance between the cone and tip. 3. When tested by applying the spraying method of between-the-row, the nozzle pressure did not affect the uniformity of spray droplet distribution. However, the nozzle pressure had a positive effect on the penetrating-reaching distance of the facing side of the rice stem and did not affect much on that of the opposite face of the stem. It was also found that the maximum pressure to affect the penetration-reaching distance was about $10kg/cm^2$. 4. The uniformity of the spray droplet distribution in the between-the-row system was greatly affected by the height and orientation of the nozzles in the boom. Based on experimental work for the different type of the boom-with-nozzle arrangements, it is recommended that the position of nozzle is set at about 0.45 m above the ground and two nozzles in the boom are oriented to be faced with each other with some angle such that the droplet stream from the nozzle would not directly face with each other.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.111-111
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• 2003

Several fungal isolates were isolated from diseased monochoria(Monochoria vaginalis, weed of paddy field), which has an resistance to sulfonyl urea(S.U.) herbicide, and were evaluated in the laboratory and greenhouse as potential mycoherbicide. Eight fungi, Alternaria sp., Colletotrichum sp., Curvularia sp., Paenicillium sp and etc. were observed in the isolates. Pathogenicity testing were done on the monochorias in the greenhouse. Monochorias were inoculated with suspensions containing conidia of each isolate at the rates of 1.0 ${\times}$ 10$\^$5/ conidia/ml and 0.1％ Tween 80 with hand-gun sprayer. Curvularia sp. and an unidentified fungal isolate caused 90-95％ mortality on the monochorias 15∼20 days after inoculation. However the other isolates induced slight symptom of disease on the monochorias. In the early stage of disease development sun-burn appearance was shown at the infected site and the last infected leaves and stems were withered to death. Subsequently the pathogenicity on the rice was evaluated with above two effective isolates. From the test an unidentified isolate showed pathogenicity on the rice but Curvularia sp., named as DBB2003, didn't. Now the mass production and formulation using Curvularia sp. DBB2003 are in progress and the field test will be followed. Combination product with Curvularia sp. DBB2003 and chemical herbicide will be more effect to control the monochoria resisted on S.U. herbicide and need to be further tested.

• Environmental Mutagens and Carcinogens
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• v.21 no.2
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• pp.128-134
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• 2001

Single cell gel electrophoresis (SCGE) assay, also called comet assay, is a rapid and sensitive method to detect DNA damage in single cell level. To evaluate the DNA damage of lymphocytes of pesticides sprayers, SCGE assay was carried out for 50 pesticides sprayer and 58 control subjects. They were interviewed with structured questionnaire to get the information about the kinds and amount of pesticide. Insecticides and fungicides were predominant among pesticides. Major components of pesticides were organophosphorus, organosulfate, cartap, carbamates, and triazole. Sprayed pesticides were classified into two groups. Group I included organophosphorus, organoarsenic, organotin, tetrazine, triazole and gramoxone, which were known to cause DNA damages. Group II pesticide were carbamates, surfactants, organosulfates, etc., which were not found as DNA damaging agents in scientific documents. Olive tail moments of 100 lymphocytes were measured by KOMET 3.1 program for each person. The means of tail moments were compared between farmers exposed to pesticides and control subjects. Farmers showed higher tail moments than control subjects (2.07$\pm$1.40 vs 1.53$\pm$0.77, p<0.05). The means of tail moments also were compared among group I sprayers (n=36), group II sprayers (n=24) and, control subject, and the means or tail moments were 3.4s$\pm$3.2o, 2.66$\pm$2.20 and 1.53$\pm$0.77 respectively. The difference between means of group I sprayers and controls was statistically significant (p<0.05). In conclusion, this study showed higher DNA damage in farmers exposed to pesticides than control subjects, and comet assay could be useful as a biological monitoring method of genotoxic pesticides for farmers.

• Korean Journal of Veterinary Research
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• v.40 no.3
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• pp.563-573
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• 2000

Periodic outbreaks of Newcastle disease (ND) caused by velogenic viscerotropic ND virus (vvNDV) has become a major concern in Korea nowadays. Throughout last epidemic, the winter season in 2000, most chicken flocks infected early, under 2-4 weeks of age, showed high mortality up to 50-100%. Serum samples collected from 201 breeder, 284 layer and 112 broiler chicken flocks were examined to evaluate the efficacy of various vaccination methods and programs routinely used for mass vaccination in the field poultry farms. Despite repeated live vaccination, most poultry flocks vaccinated by drinking water route using nipple water supply system failed to produce solid active immune response to NDV during the growing time. In the present study, we applied the spray vaccination technique using Ulvavac or Desvac sprayer to the experimental poultry flocks and examined the efficacy of live vaccination effects induced by it under field condition. Measurable antibody to NDV as well as early protection against vvNDV challenge were found in poultry flocks vaccinated by spray route. Further, we did not found significant post vaccination reactions caused by spray vaccination if properly administered. These data indicate that the spray vaccination will be safe and reliable mass vaccination method for the prevention of ND.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.487-494
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• 1993

In order to establish a fully automatized pest control in the a greenhouse , the authors developed a prototype of microcomputer installed spraying vehicle which traveled along the furrows. Since a power sprayer mounted on the vehicle was driven by gasoline engine, plants grown in the greenhouse might be injured by the gas exhausted from the engine. Thus , effects of exhausted gas on photosynthetic rate and the shedding of flowers and buds of plants were examined. At first, effects of exhausted gas on photosynthetic rate of potted sweet pepper (Capsicum annuum L.) and eggplant(Solanum melongena L.) plants were examined. In a closed vinyl house the engine was operated for 5 minutes and plants were exposed to the gas for 2hours in the daytime on a fine day. Photosynthetic rate did not significantly decreased by the treatment in both species. Secondly, effects of ehtylene on the shedding of flowers and buds of sesame (Sesamum indicum L. ) were examined. In the closed and partiall opened vinyl house, the engine was operated for 5 minutes and potted sesame plants were exposed to the gas for 12 hours in the night. In partially opened vinyl house, ethylene concentration decreased to 0 ppm 3 hours after the engine was stopped and flower and bud did not shed. In contrast, when vinyl house was closed ethylene concentration was 0.75 pm even 12 hours after the engine was stopped and flowers and buds shed markedly and epinasty was observed in upper young leaves. As mentioned above , it was revealed that injury of plants in the greenhouse caused by the gas exhausted from a gasoline engine could be prevented by providing suitable ventilation.

• Journal of Biosystems Engineering
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• v.39 no.4
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• pp.330-335
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• 2014

Purpose: This study was conducted for enhancing the performance of a conventional quick freezer by introducing the supercooling state, using a low-temperature coolant. Methods: In the present investigation, the supercooling process was executed prior to quick freezing for reducing the time by which the temperature passes the zone of maximum ice crystal formation. Every food has different nucleation points and hence, we used silicone oil as the coolant for supercooling for easy modification of temperature. Additionally, for quick freezing, we used liquid nitrogen spray. Results: Using the heat exchanger-type precooler with silicone oil, the temperature of the chamber was easily changed for enabling supercooling. Particularly, the results of the freezing test with garlic indicated that this system improved the hardness of garlic after it was thawed, compared to the conventional freezing method. Conclusions: Before quick freezing, if the food item is subjected to the supercooling state, the time from nucleation to the temperature reaching the frozen state ($-5^{\circ}C$, which is the maximum ice crystal formation zone) will be shorter than that incurred using quick freezing alone. The combination of the heat exchanger-type supercooler and liquid nitrogen sprayer is expected to serve as a promising technology for improving the physicochemical qualities of frozen foods.

• KIEAE Journal
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• v.15 no.3
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• pp.5-14
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• 2015

Purpose: Recently, according to climate changes, human health is exposed to danger over the world and they influence all fields of human society. Due to these climate changes, humans can be exposed to more frequent and extreme scorching heat and cold wave than the present. As precautions against these urban higher temperature and dryness, diverse methods are being sought. Among them, as measures to form cold islands, the evaporative cooling effect realistic to social and economic conditions was examined. Method: This study was conducted to analyze effects of temperature reduction and cooling according to injection quantity of minute water particles by using a blast sprayer as one of alternatives of alleviation of urban climate changes in outside space in summer. For this, through temperature difference in accordance with the injection quantity per hour of a day, a time zone representing the value of the highest temperature change was analyzed. Also, by analyzing temperature difference according to the injection quantity per daytime insolation, relation of amounts of insolation and evaporation was investigated. Temperature difference in accordance with distances at the highest temperature with the highest value in temperature changes was analyzed. Result: At the study result, about temperature, as injection quantity increase, temperature reduction was significant statistically at the highest temperature with the most insolation. A factor with the highest influence was judged to be the increase of the injection quantity. According to the injection quantity, it was predicted that $3.1^{\circ}C$ temperature reduction of a daily average in case of 0.16L/min, $3.5^{\circ}C$ temperature reduction of a daily average in case of 0.32L/min, and $4.4^{\circ}C$ temperature reduction of a daily average in case of 0.48L/min.

• Horticultural Science & Technology
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• v.31 no.2
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• pp.239-245
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• 2013

Occupational exposure and risk assessment were conducted to evaluate the safety of operators when insecticide acetamiprid was applied to apple orchard using a speed sprayer. Dermal patches, cotton gloves, socks, and masks were used to monitor the dermal exposure, and personal air pump with solid sorbent was used to measure the potential inhalation exposure. In validation to analytical methods, the limit of detection and limit of quantitation were 0.25 ng and 1 ng, respectively. Good reproducibility (coefficient variation < 4%), linearity (coefficient determination > 0.999), and recovery (85.3-118.2%) were obtained. Trapping efficiency of solid sorbent was 96.4% while breakthrough did not occur. Only hand exposure was measured on the gloves during mixing/loading to give $33-1,132{\mu}g$. Exposure amount of operator 3 among 4 workers was noticeably high. The total volumes of spray liquid for operators were $535-1,235mL{\cdot}h^{-1}$, corresponding to 0.03-0.08% of the applied spray solution. Highest contaminated parts of body were thighs, chest, and lower legs. The inhalation exposure ratio to the total application amount was significantly low. However, wind seemed to affect the inhalation exposure of operator. For risk assessment, margin of safety was calculated by the application of cloth and dermal penetration rate to obtain values of much larger than 1 in all cases. Therefore, health risk of operators during treatment of acetamiprid in apple orchard could be of least possibility.

• Korean Journal of Food Science and Technology
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• v.20 no.3
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• pp.326-330
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• 1988

For the automation of Koji incubation process, microcomputer based Koji incubation system was built and applied to acquisition of the process variables, and to control of the Koji incubation process. The incubation variables included the relative humidity and Koji weight. And data measured were sent to the microcomputer by the interface device built with MC 6821 PIA. Incubation environment conditions -temperature and humidity- were controlled by the actuation of heater and mist sprayer with on/off signal generated by ASIC program. Aspergillus oryzae as a starter of the Koji and steamed barley as media were used and Koji was successfully manufactured both at $25^{\circ}C,\;70%$ RH and at $27^{\circ}C,\;80%$ RH. During the Koji preperation, the temperature was linearly increased and substrate was consumed stepwise showing 3 steps in the weight loss curve.