• Journal of Microbiology
• /
• v.39 no.4
• /
• pp.286-292
• /
• 2001

During mitosis. the proper segregation of duplicated chromosomes is corrdinated by a spindle check-point. The bifurcated spindle checkpoint blocks cell cycle progression at metaphase by monitoring unattached kinetochores and inhibits mitotic exit in response to the misorientation of the mitotic spin- dle Ibd1p/Bfa1p is a spindle checkpoint regulator of budding yeast in the Bub2p checkpoint pathway for mitotic exit and its disruption abolishes mitotic arrest when proper organization of the mitotic spin-dls inhibited. Ibd1p/Bfa1p localizes to the spindle pole body, a microtublue-organizing center in yeast, and its overexpression arrests the cell cycle in 80% of cells with an enlarged budy at mitosis and in 20 % of cells with multiple buds. In this study, we found that the C-terminus of Ibd1p/Bfa1p phys-ically interacts with Skp1p, a key component of SCF (Skp1/cullin/F-box) complex for ubiquition-medi-ated proteolysis of cel cycle regulatores as well as an evolutionally conserved kinetochore protein for cell cycle progression. A putative F-box motif was found in the C-terminus of Ibd1p/Bfa1p and its function was investigated by making mutants of conserved residues in the motif. These Ibd1p/Bfa1p mutants of a putative F-box interacted with SKp1p in vitro by two-hybrid assays as wild type Ibd1p/Bfa1p. Also these Ibd1p/Bfa1p utants displayed the overexpression phenotypes of wild type Ibd1p, when over-expressed under inducible promoters . These results suggest that a putative F-box motif of Ibd1p/Bfa1p is not essential for the interaction with SKp1p and its function in mitotic exit and cytokinesis.

• Journal of Life Science
• /
• v.32 no.2
• /
• pp.161-166
• /
• 2022

Mitosis is a process in which a replicated genome is distributed to two daughter cells, and it is necessary for cell survival and organismal development. During mitosis, the spindle assembly checkpoint (SAC) ensures faithful chromosome segregation by monitoring the kinetochore attachment to the mitotic spindle. Although the SAC mechanism has been extensively studied over the last 30 years, the mechanism by which a single unattached kinetochore activates the SAC remains unclear. The key components of the SAC are Mad1, Mad2, Mad3 (BubR1 in higher eukaryotes), Bub1, Bub3, and Cdc20, which are all required for SAC activation. An essential step for SAC activation is the formation of the Mad2 - Cdc20 complex in the unattached kinetochore, which is kinetically disfavored. Although the mechanism by which Mad2 and Cdc20 are recruited to unattached kinetochores is well-known, it is not clear how they form a complex. Recently, a key mechanism for the formation of the Mad2 - Cdc20 complex has been identified, which is catalyzed by an unattached kinetochore. This supports the evidence that a single unattached kinetochore can activate the SAC signaling. Herein, we discuss the known key mechanism for SAC activation, review the recent studies on SAC, and conclude how their discoveries improved the understanding of mitosis.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2002.05a
• /
• pp.382-386
• /
• 2002

The tapping is machining process that makes a female screw on the parts to be assembly together. It is used for the high-speed tapping machine with synchronizing function for the high productivity. This paper describes the development of the ultra high-speed tapping machine with 10,000rpm. The key factors in the tapping speed are the acceleration/deceleration velocity and the synchronizing errors between the spindle motor and feeding motor. To minimizing acceleration/deceleration time, the low inertia spindle with synchronous built-in servo motor is developed. To minimizing synchronizing errors, the tapping cycle algorithm under open architecture CNC environment is optimized. The developed tapping machine has 0.13sec/10,000rpm in acceleration/deceleration time and the synchronizing error below 4.0%. It has 0.55sec for cycle time of one female screw, M3 tap, 2 times depth of tap diameter.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2000.06a
• /
• pp.61-70
• /
• 2000

This paper described the current status of machine tool technology and its future trends with a particular emphasis on high-speed machining. People in machine tool industry have continuously sought to serve fast-changing manufacturing industry with economical machining solutins. At presents, it appears that more productivity gain is demanded to shorten time-to-market and machining requirements become more stringent. In this regard, this paper firstly addressed a high-speed spindle as a key element for the next-generation machine tools. The sequel to it apparently went to high-speed feed axes and final discussion included the problem of how to optimize overall system including servo function. Lastly a brief look to NC technology including machine intelligence was taken.

• Development and Reproduction
• /
• v.18 no.2
• /
• pp.117-125
• /
• 2014

Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than that of the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome systems of mature oocyte. To get the best way for keeping competence of matured oocytes, we studied the best conditions for vitrification focused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzed the survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same between groups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration group and the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimental groups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 min equilibration group showed best condition compared with the other groups. Based on these results, the equilibration time is one of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effects of physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimal equilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight into intracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of human oocyte.

• Molecules and Cells
• /
• v.39 no.9
• /
• pp.654-662
• /
• 2016

Almost all eukaryotic proteins are subject to post-translational modifications during mitosis and cell cycle, and in particular, reversible phosphorylation being a key event. The recent use of high-throughput experimental analyses has revealed that more than 70% of all eukaryotic proteins are regulated by phosphorylation; however, the mechanism of dephosphorylation, counteracting phosphorylation, is relatively unknown. Recent discoveries have shown that many of the protein phosphatases are involved in the temporal and spatial control of mitotic events, such as mitotic entry, mitotic spindle assembly, chromosome architecture changes and cohesion, and mitotic exit. This implies that certain phosphatases are tightly regulated for timely dephosphorylation of key mitotic phosphoproteins and are essential for control of various mitotic processes. This review describes the physiological and pathological roles of mitotic phosphatases, as well as the versatile role of various protein phosphatases in several mitotic events.

• Journal of the Korean Society for Precision Engineering
• /
• v.19 no.11
• /
• pp.221-227
• /
• 2002

Tapping is a machining process that makes a female screw on parts to be assembly together. Recently, as the number of small and compact products increases the radius of tap as small as 1 mm is not unusual and more accurate tapping is needed. In complying with those needs, some high-speed tapping machines with synchronizing function have been developed. This paper describes the development of an ultra high-speed tapping machine up to 10,000rpm. The key factors in the tapping speed are the acceleration/deceleration and the synchronizing errors between spindle motor and fred motor. To minimize the acceleration/deceleration time, a low inertia spindle with a synchronous built-in servo motor was developed. To minimize the synchronizing errors, the tapping cycle algorithm was optimized on an open architecture CNC. The developed tapping machine has the acceleration/deceleration time of 0.13sec/10,000rpm for rigid tapping and the synchronizing error below 4.4%. The cycle time for tapping a female screw of M3 and depth 2 times diameter was 0.55sec.

• BMB Reports
• /
• v.40 no.6
• /
• pp.973-978
• /
• 2007

Septins are a family of conserved cytoskeletal GTPase forming heteropolymeric filamentous structure in interphase cells, however, the mechanism of assembly are largely unknown. Here we described the characterization of SEPT12, sharing closest homology to SEPT3 and SEPT9. It was revealed that subcelluar localization of SEPT12 varied at interphase and mitotic phase. While SEPT12 formed filamentous structures at interphase, it was localized to the central spindle and to midbody during anaphase and cytokinesis, respectively. In addition, we found that SEPT12 can interact with SEPT6 in vitro and in vivo, and this interaction was independent of the coiled coil domain of SEPT6. Further, co-expression of SEPT12 altered the filamentous structure of SEPT6 in Hela cells. Therefore, our result showed that the interaction between different septins may affect the septin filament structure.

• Journal of Veterinary Science
• /
• v.24 no.1
• /
• pp.4.1-4.16
• /
• 2023

Background: In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E₂) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. Conclusions: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 1992.10a
• /
• pp.47-51
• /
• 1992

Automatic detection of tool breakage during NC machining is a key issue not only for improving productivity but to implement the unattended manufacturing system. In this paper, we develop a vibration sensor-based tool breakage detection system for NC milling processes. The system obtains the time-domain vibration signal from the sensor attached on the spindle bracket of our CNC machine and declares tool failures through the on-line monitoring schemes. For on-line detection, our approach is to use the PSC(statistical process control) methods being increasingly used for on-line process control. The main thrust of this paper is to propose and compare the performance of SPC methods including : a) X-bar control scheme, b) S control scheme, c)EWMA (exponentially weighted moving average) scheme, and d) AEWMA (adaptive exponentially weighted moving average) scheme. The performance of the control schemes are compared in terms of the type 1 and 2 error calculated from the experiment data.