• Journal of the Korean Society of Marine Environment & Safety
• /
• v.23 no.2
• /
• pp.177-186
• /
• 2017

This paper attempts to quantitatively assess the public value that has been assigned to this government plan by employing a contingent valuation technique. Data gathering performed that professional research firm administrated a face-to-face national survey of 1,000 randomly-selected households. A one-and-one-half-bound model was adopted to elicit willingness to pay (WTP) responses, and the payment vehicle used was income tax. The WTP model used in this study is based on a utility difference approach and the spike model. The results showed that the public value of conserving green turtles was estimated to be 2,570 Korean Won per household over the next 10 years as of 2016, statistically significant at the 1% level. Expanding the values considered to include the national population gives a public value of 48.7 billion Korean Won. Thus, the public value of rescuing, rehabilitating, and releasing green turtles that have been caught by fishermen, collided with fishing boats, and died in nets through the government program is not small.

• Korean Journal of Food Science and Technology
• /
• v.44 no.1
• /
• pp.1-7
• /
• 2012

There have been few studies related to ELISA for mackerel. In this study we developed a sandwich ELISA for mackerel in processed foods using rabbit polyclonal antibodies against mackerel parvalbumin, the major allergen of mackerel and heat-stable protein. The parvalbumin was purified by ammonium sulfate precipitation and Sephadex G-50 column chromatography. From the standard ELISA curves, the detection limit of parvalbumin was 3 ng/mL and the detection range of mackerel was 5-5,000 ${mu}g/mL$. We further investigated the cross-reactivity of the antibodies toward mackerel, mackerel pike, salmon, flatfish, armorclad rockfish, cod fish, squid, shrimp, blue crab, and lobster. The antibodies were specific for mackerel only. The mean assay recoveries in cooked cream soup, baby food, sausage, and sauce spiked with 0.01 to 0.3% mackerel were 104, 101, 54, and 0%, respectively. In sample tests of 16 commercial items, the qualitative coincidence ratio of assay result and indication was 75%.

• The Korean Journal of Ecology
• /
• v.28 no.2
• /
• pp.99-103
• /
• 2005

Actual vegetation map drown with the scale 1 to 100 and Alnus japonica population in Ja-neup and Woong-neup of the Mujechi moor were described in order to monitor long-termly and preserve permanently, where is a very rare Molinietea moor and a legally protected area. A total of 3036 plots of 5m $\times$ 5m were surveyed during summer 1996. Thiessen polygons of 1491 alder trees were derived from the plot data. Actual vegetation map was illustrated by 6 cover types such as needle spike-rush type, moor-grass type, alder-moor type, eulalia type, oak forest type, and exposed site. Molinia grasses native to the moor and Miscanthus grasses alien to the moor are reciprocally dominant. The area of Molinia grasslands was rapidly in decline and alder population size was dramatically in increase in the moor, particularly in Woong-neup. In Molinietea moor preservation more attention should be focused on the regulation of a nutrient rich soil from forest road and fire.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2004.04a
• /
• pp.12-27
• /
• 2004

Thanks to spectacular advances in the techniques for identifying proteins separated by two-dimensional electrophoresis and in methods for large-scale analysis of proteome variations, proteomics is becoming an essential methodology in various fields of plant sciences. Plant proteomics would be most useful when combined with other functional genomics tools and approaches. A combination of microarray and proteomics analysis will indicate whether gene regulation is controlled at the level of transcription or translation and protein accumulation. In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is a most prevalent technique to identify rapidly a large of proteins in proteome analysis. However, the conventional Western blotting/sequencing technique us still used in many laboratories. As a first step to efficiently construct protein data-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein spots are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins (i. e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 30% of total rice cDNA have been deposited in the database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that fumed out to be calreticulin, gibberellin-binding protein, which is ribulose-1,5-bisphosphate carboxylase/oxygenase activate in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins (http://genome .c .kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Recently, we are separated proteins from grain filling and seed maturation in rice to perform ESI-Q-TOF/MS and MALDI-TOF/MS. This experiment shows a possibility to easily and rapidly identify a number of 2-DE separated proteins of rice by ESI-Q-TOF/MS and MALDI-TOF/MS. Therefore, the Information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful in the plant molecular breeding. Also, information from our study could provide a venue to plant breeder and molecular biologist to design their research strategies precisely.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.2
• /
• pp.184-191
• /
• 1989

The effect of various supplementary light sources on the agronomic characteristics has been investigeted for generation acceleration of barley in green house with the condition of 15$^{\circ}C$ and 8 hrs. day light followed by each supplementary light sources including incandescent electric lamp (IN), Blue fluorescent lamp(BF). day-light fluorescent lamp(DF), Biolux lamp(BL), BL+BF, In+BF and IN+BL. Leaf number of main stem, leaf area, culm length, dry matter yield of shoot, number of grain per spike, grain yield per plant were most sharply decreased with the exposition of IN, while flag-leaf emergence on main stem and I, 000 grain weight were earlist and heavist respectively with the exposition of IN. In appeared to be best light source for shortening the days to heading, but IN + BF will be most desirable light source with considering both days to heading and adequate yield at the same time.

• Korean Journal of Environmental Biology
• /
• v.32 no.1
• /
• pp.49-57
• /
• 2014

Orb-web spiders can be considered as an effective bio-indicative animal to monitor the ecological air pollution of certain habitat since they have limited shifting ability during their life spans. In this study we revealed the fine structural characteristics of booklung in the orb-web spider Nephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikes near the atrium could be act as an effective filtering structure for dust materials. Furthermore, inner cuticular spikes which protruded across the air sacs, have the functions not only sustaining appropriate respiratory volume of each air sac but also help to enhance respiratory capacity by maximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, the asbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore, histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixed on the hemolymph space. In addition, although accumulated mass of hemocytes were observed near the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surface of the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly, these results indicate the spider's booklung could be valuable tool in the detection ecological air pollutants.

• Conservation Science in Museum
• /
• v.3
• /
• pp.65-69
• /
• 2001

Scientific analysis are carried on gilded ornamental shoes, which have been excavated from tomb No 1. and No 4. in Beopcheon-ri, Wonju dated from Baekje Period. This object is a very important because it provides valuable information on the development of metal-making techniques of that period. Thus, this article illustrates the investigation conducted to reveal how the object was created using what materials and techniques. Instead of the spikes-attached to the bottom plate of the object-that couldn't be sampled, a sample of a decorative rivet showing a similar structure to the spikes-attached to the other parts of the shoes-was prepared for a cross-section examination. Using radiography and microscopes, it was found that the head of a spike was placed and then punched to the gilded plate, so it can be held to the shoes. Under the SEM-EDS, the cross-section of the rivet shows that the gilding layer was applied before the attachment of the rivets. It also shows that the gilding layer is distributed unevenly and there are empty spaces indicating amalgam gilding was employed. This was confirmed as Mercury was detected on the SEM-EDS analysis of the object. The examination of the microstructure of the plate using the SEM-EDS revealed that the object is made of a single copper alloy plate with recrystallized twining and non-metallic white inclusions, which found to be lead in this case.

• FLOWER RESEARCH JOURNAL
• /
• v.16 no.1
• /
• pp.44-48
• /
• 2008

This experiment was conducted to examine the effect of injecting cool air from an abandoned mine during the summer time for the growth of Doritaenopsis. The air temperature of abandoned mine in Boryeong was $12{\sim}14^{\circ}C$. The day and night temperatures were set at $22^{\circ}C$ and $20^{\circ}C$, respectively, from June to August in the experimental plastic house. This temperature range was within the suitable range for floral induction in Doritaenopsis. Average outside temperature was $28.4{\sim}32.8^{\circ}C$. The 3% of the crop developed flower stalk in 20 days after the treatment initiation, 65% in 45 days, and 100% in 90 days. The flower stalk length was short (48.7cm) in 30 days and long (62.4cm) in 60 days of treatment. The flower stalk length became longer as time passed. Flower spike and number of florets per stalk displayed the same tendency. Number of nodes was 6~7 and was not affected by the period. The first blooming appeared on 15th of September at 45 days and blooming tended to appear late as the period is lengthened. When the cool air from an abandoned mine was injected, the crop formed flower stalk three months earlier and bloomed four months earlier than the untreated control.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.64 no.3
• /
• pp.234-245
• /
• 2019

In order to select the best varieties and cultivation methods for the production of domestic wheat for Hodugwaja(a walnut shaped confection), we carried out a 3-year experiment (2015~2017) to investigate the effect of different factors (crop variety, planting date, nitrogen fertilization) in a double cropping system (wheat then rice) on crop yields in the Cheonan area. Rice is the second crop in the system, and requires an accumulated temperature for 40 days of about $840{\sim}930^{\circ}C$ to ensure ripening. Transplanting dates for rice between June 29 and July 6 were suitable; transplanting on or after July 13 does not ensure ripening. The daily hours of sunshine ranged from 6.3 to 6.5 hours, which were slightly higher than the optimum of 6.0 to 6.1 hours. The higher the nitrogen fertilizer treatment, the higher culm length, and spike length of wheat. The yield of wheat per 10a tended to increase as the amount of nitrogen fertilizer increased. Wheat yields were highest for the Sooan variety, followed by Goso, followed by Keumgang. The number of days from transplanting to heading of rice were shortest for the Jopyeong variety followed by Unkwang, followed by Haedam. The yield of milled rice per 10a increased as the transplanting date was earlier and the transplanting date of June 9 showed the average yield of more than 500 kg in three varieties. From the results of the experiment, we recommend the Goso wheat variety and the Haedam rice variety for growing in a double cropping system under the climatic conditions of the Cheonan area.

• Nuclear Engineering and Technology
• /
• v.16 no.3
• /
• pp.141-154
• /
• 1984

An analysis is presented of key phenomena and scenario which imply some general trends for beyond design-basis-accident in Kori-1 PWR dry containment. The study covers a wide range of severe accident sequences initiated by small break LOCA. The MARCH computer code, with KAERI modifications was used in this analysis. The major emphasis of the paper are two folds, 1) the phenomenologic understanding of severe accident and 2) a study of H2 combustion and debris/ water interactions in a specific small break LOCA for Kori-1 plant. The sensitivity studies for the specific plant data and thermal interaction modelings used in the SASA were performed. The results show that if hydrogen burning does occur at low concentration, the resulting peak pressure does not exceed the design value, while the lower concentration assumption results in repeated burning due to the continuing H$_2$ generation. For debris/water interaction, the particle size has no effect on the magnitude of peak pressure for the amount of water assumed to be in the reactor cavity. But, the occurrence of peak pressure is considerably delayed in case of using the dryout correlation. The peak containment pressure predicted from the hydrogen combustion and steam pressure spite during full core meltdown scenario does not present a severe threat to the containment integrity.