• Title/Summary/Keyword: Spermatocyst

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Seminiferous Epithelium Cycle in Bombina orientalis

  • Yi, Min-Joo;Lee, Jung-Hun
    • Development and Reproduction
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    • v.19 no.1
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    • pp.1-10
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    • 2015
  • The purpose of the present study was to examine the seminiferous epithelium cycle of Bombina orientalis using a light microscope. The cycle was divided into a total of 10 stages, according to the morphological characteristics of the cells. The spermatogenetic cells included primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes, spermatid and sperm. At stage I, the primary spermatogonia was located closer to basal lamina of the seminiferous tubule without spermatocyst formations. Especially at the stage II, the secondary spermatogonia were located in the spermatocyst. The primary and secondary spermatocytes were found from stages III to VI. The secondary spermatocytes were smaller in size than the primary spermatocytes, but they had thicker nucleoplasm and smaller nuclei. The round-shaped, early sperm cells were formed in stage VII, and further divided at stage VIII to have more concentrated nucleoplasm before division to matured sperm cells. At stage X, the matured sperm cells emerged from the spermatocyst. Considering the above results, this study presented the special characteristics in the generation and type of sperm formation. The germ cell formation occurred in various stages, like the perspectives of Franca et al (1999), ultimately, providing taxonomically useful information.

Glycogen Effect of the Sperm Maturation during the Spermiogenesis of Rana catesbeiana (황소개구리 (Rana catesbeiana)의 정자변태과정 중 글리코겐이 정자 성숙에 미치는 역할)

  • Go, Song-Haang;Lee, Jung-Hun
    • Applied Microscopy
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    • v.31 no.3
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    • pp.257-266
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    • 2001
  • To investigate the process of spermoigenesis and glycogen effect during the spermatogenesis of Rana catesbeiana, the morphological characteristics of the testes were examined by light and transmission electron microscopy. Spermiogenesis of R. catesbeiana was divided into three stages on the basis of the features of the nucleus and the cytoplasm organelles. Except for the primary spermatogonia, the phases from the spermatocytogenesis to the spermatids before spermiation phase were surrounded by spermatocyst. Especially , the glycogen particles were not observed until in the stage of spermatocytogenesis, but from the early spermatids to the maturation phase were observed in the nucleus, acrosome and cytoplasm of the spermatids. The present result suggests that the glycogen may play an important role in the sperm maturation, and as a source of the energy in the wave-movement of sperm tail.

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Differentiation of Seminiferous Epithelium and Spermiogenesis in the Testis of Rana catesbeiana (황소개구리(Rana catesbeiana)의 세정관 상피의 분화와 정자변태)

  • Go, Song-Haang;Lee, Jung-Hun
    • Applied Microscopy
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    • v.31 no.2
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    • pp.143-156
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    • 2001
  • The aim of this study was to clarify the differentiation of seminiferous epithelial cells and spermiogenesis in the testis of Rana catesbeiana. Spermatogenesis of R. catesbeiana consists of primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids. They were subdivided into eight stages on the basis of the morphological features of the germ cell differentiation. From the spermatocytes except primary spermatogonia to before the spermiation of spermatids were surrounded by spermatocyst. Spermiogenesis of R. catesbeiana can also be divided into three stages on the basis of morphological features of the nucleus and the cytoplasm organelles. Spermatozoon contained a saccular acrosome, a cylindrical and tapered slighty at both ends head, and a tail with only the axoneme.

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The Ultrastructure of Testis and Spermatogenesis in Bluespotted Mud Hopper(Boleophthalmus pectinirostris) (짱뚱어, Boleophthalmus pectinirostris 정소의 미세구조 및 정자형성)

  • Kang Kyoung Ho;Kho Kang Hee;Kim Jae Min
    • Development and Reproduction
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    • v.7 no.2
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    • pp.89-93
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    • 2003
  • The present study observed the ultrastructure of testis of bluespotted mud hopper(Boleophthalmus pectinirostris), and sperrnatogenesis was discussed also. The testis was surrounded by a thin adventitia, inside which spermatocyst composed the parenchyma of testis. Each lobule was enwrapped by many spermatocysts, which were filled with different kinds of spermatogenic cell clusters at the same developmental stage. In the lobule lumen there are large numbers of spermatozoa The thin adventitia(outer wall) of testis was composed of outer epithelium, and the underlying layers, such as collagen fiber layer, and myoid tissue. The myoid tissue elongated into the inside of testis, became the main componentof interstitium between spermatocyst where sperrnatogenesis occurred. In addition interstitial cells containing dense homogeneous nucleus and abundant mitochondria were observed. Spermatogonia contained round nucleus with diffuse chromatin and nucleolus, and dense nuclear bodies surround by mitochondria in cytoplasm. The synaptonemal . complex was observed in primary spermatocytes clearly. Early spermatid presented larger round nucleus composed of granular chromatin, which was located in the center of cytoplasm. The nucleus of mid-spermatid composed of finely granular chromatin lied on one side of spermatid, and abundant mitochondria had migrated another side. A nuclear fossa appeared in the site near mitochondria in late-spermatid, and the centriole was formed in nuclear fossa.

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Spermatogenesis and Chromosome Number of the Tobacco Budworm Helicoverpa assulta Guenee (Lepidoptera: Noctuidae) (담배나방(Helicoverpa assulta: 인시목 밤나방과)의 정자형성 과정과 염색체수)

  • 유종명;정성은;박희윤;채순용;김상석
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.1
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    • pp.12-20
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    • 1996
  • The spermatogenesis and chromosome number were investigated in the pupal testes of Helicouerpa assulta Guenee by light microscopy. During the spermatogenesis, each bundle of P8(256) sperms developed by 6 mitotic and 2 meiotic spermatogonial divisions. From the early stage of spermatogenesis, it was distinguishable between two kinds of sperm differentiation, eupyrene and apyrene spermatogenesis, which are characteristic in Lepidoptera, by the differences in nuclear shape and cell distribution in immature spermatocyst. Through the followed spermiogenesis, the spermatocysts were developed into two kinds of mature cyst, a streamline-shaped eupyrene cyst with nucleated sperms of thready head or a long spindle-shaped apyrene cyst with anucleated sperms of cylindrical head. As the results off chromosomal analysis at metaphase of the spermatogonial mitosis and spermatocytic meiosis, the chromosome number were 2n=6a/n=31, respectively, and no variation between individuals.

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