• 한국수정란이식학회지
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• 제10권3호
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• pp.243-250
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• 1995

Frozen storage of the oocytes has been used in a few mammalian species including mouse, hamster, human and cattle. However, frozen4hawed oocvtes show different sperm penetration on the levels of the zona pellucida and the plasma memhrane when compared with fresh oocytes. To elucidate biological changes occurring during freezing and thawing, we examined the kinetics of sperm penetration into frozen-thawed hamster oocytes. Oocytes obtained from superovulated female golden hamsters were frozen-thawed in an autofreezer according to an established method. Fresh and frozen4hawed oocytes were fertilized in vitro with capacitated hamster spermatozoa after removing the zona pellucida. The oocytes were examined at 1, 2, 3 and 6 h postinsemination. Sperm penetration found to be 1 h delayed in frozen-thawed oocytes. Other parameters such as degree of polyspermy and decondensing sperm heads were not affected by freezing and thawing. The results suggest that freezing and thawing may cause changes in the egg membrane surface and subsequently which leads to delay in the sperm-egg fusion.

• Journal of Animal Science and Technology
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• 제63권1호
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• pp.46-57
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• 2021

Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon (Citrullus lanatus) fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1-7. Pooled semen was obtained from fertile roosters and equilibrated with diluents at ratio 1:2 in the various treatments and were evaluated using computer software coupled microscope and seminal oxidative status assay. 168 laying hens randomly divided into 7 treatment of 8 replicates and 3 hen per replicate. Hen were everted, and semen (2 × 108 Spermatozoa) deposited intra-vagina and eggs collected over 8 weeks to assess fertility and hatchability of eggs laid. The result obtained revealed that watermelon-dextrose saline rooster semen diluent enhanced progressive motility, sperm kinetics and lowered non-progressive motility in T2-T6 compared to T7 over the 3 hours of evaluation. Watermelon addition to rooster semen diluent enhance the antioxidant capacity of rooster semen and lowered lipid peroxide generation. The percentage fertility was highest in T3 (81.01%) and T4 (81.24%) with lowest value obtained in T7 (73.46%). The hatchability of eggs set of hens inseminated with undiluted semen (71.46%) was lower than values for hens inseminated with watermelon inclusive extended semen (75.71%-80.39%). The optimal inclusion of 30%-40% watermelon in dextrose saline diluent enhance rooster semen kinetics, seminal oxidative stability and egg fertility.

• Clinical and Experimental Reproductive Medicine
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• 제30권1호
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• pp.105-109
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• 2003

Objective s: To assess the fertilizing capacity using sperm penetration assay (SPA) to predict the outcome of the in vitro fertilization-embryo transfer (IVF-ET) outcome. Materials and Methods: Semen samples were provided by 129 patients undergoing IVF. We attempted to correlate the extent of sperm penetration under enhanced SPA protocol with the results of fertilization, cleavage, preimplantation embryo development, and pregnancy. Results: Univariate analysis demonstrated a statistically significant correlation between fertilizing capacity and motility, kinetics, fertilization, cleavage and embryo development, and pregnancy rate. By logistic regression analysis, fertilizing capacity was found to be the only variable that was statistically significant with respect to pregnancy rate. Fertilizing capacity, cleavage rate and pregnant rate were significantly higher in pregnant group. However, the fertilization rates was comparable with both group. Conclusions: Lower fertilizing capacity could denote a poorer prognosis for establishing a pregnancy, even after satisfactory fertilization rate is achieved.

• 한국수정란이식학회지
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• 제27권1호
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• pp.51-55
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• 2012

The objective of this study was to investigate the motility and kinematics of boar sperm that while stored at 4C. The samples of fresh boar semen were place into an extender, Androhep, and stored at $4^{\circ}C$. In three of these samples, cryoprotectants were added. The sperm's motilities and kinematics were evaluated by using microscope (${\times}400$) and the viability status was evaluated by using with eosin staining method. The 5 sample groups are; Goup A:Androhep (extender), stored at $17^{\circ}C$. Group B:Androhep (extender), stored at $4^{\circ}C$. Group C:Androhep (extender), + 3% glycerol (cryoprotectant), stored at $4^{\circ}C$. Group D:Androhep (extender), + 3% DMSO (cryoprotectant), stored at $4^{\circ}C$. Group E:Androhep (extender), + 3% ethylene glycol (cryoprotectant), stored at $4^{\circ}C$. In group A, the sperm's motility was reduced. On day one the sperm's motility was ($85.7{\pm}2.3$) and day 5 the motility was ($43.9{\pm}3.3$). In group B, C and D the sperm's motility were reduced to 0 on day 5. In group E the sperm's percentage of motility decreased. On day one the sperm's motility was ($42.0{\pm}0.5$) and day 5 the motility was ($2.3{\pm}0.3$). When comparing cryoprotectant in samples of boar sperm there is a slight improvement in the results when the use of Androhep Lite (extender), + 3% ethylene glycol (cryoprotectant), stored at $4^{\circ}C$ are used. Based on these results, ethylene glycol can protect sperm from heat shock at $4^{\circ}C$, but not satisfactory level. However, it showed the possibilities of liquid semen preservation at $4^{\circ}C$ by using cryoprotectant.

• 한국임상수의학회지
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• 제21권4호
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• pp.329-335
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• 2004

냉각이 개 정자의 기능(생존력, 활력, 첨체고유성)과 형태(기형율)에 미치는 영향을 알아보기 위해 23마리 개의 정소상체에서 정자를 회수하여 다음과 같은 실험에 사용하였다. 정자를 3% BSA가 첨가된 0.3M glucose(G-BSA)에 희석한 후 실험 1에서 서로 다른 온도, 0, 10, $37^{\circ}C$에 30분동안 냉각하여 $37^{\circ}C$에서 가온한 후 정자의 활력, 생존력, 첨체고유성을 검사하였다. 실험 2에서 $0^{\circ}C$와 4$^{\circ}C$에서 정자를 서로 다른 시간 (5, 10, 15, 30분)동안 노출시켜 $37^{\circ}C$에서 가온한 후 정자의 활력 및 생존력을 검사하였다. 또한 sugars가 개정자의 기능과 형태에 미치는 영향을 알아보기 위해 여러 종류의 sugars (0.3M)를 준비하고 $0^{\circ}C$에서 정자를 sugardyddor에 노출시켜 $37^{\circ}C$에서 가온한 후 정자의 활력, 생존력 및 기형정도를 관찰하였다. 본 실험결과 개 정조상체유래의 정자는 냉각온도가 감소할수록 정자의 기능이 급격하게 감소하여 냉각에 민감하게 나타났다.(P<0.05), 특히 정자의 활력이 다른 정자의 기능, 생존력과 첨체고유성에 비해 냉각에 민감하게 나타났다. 정자를 $0^{\circ}C$와 $4^{\circ}C$에서 서로 다른 시간(5, 10, 15, 30분) 동안 냉각하였을 때 30분 동안 냉각된 정자가 그 외의 시간 동안 냉각된 정자에 비해 생존력이 저하되었다. (P<0.05). 이상의 결과 개 정소상체유래의 정자는 냉각에 비교적 민감하였으며 정자의 냉각에 대한 민감성을 완화시키는 첨가제로 단당류뿐만 아니라 다당류에 대한 연구가 이루어져야 할 것으로 생각된다.선택에 대한 인식이 낮음을 알 수 있었다. 부페식당에서 가장 좋아하는 음식의 국적은 54.4%가 한국음식으로 나타났다. 부페식사에서 '약간' 또는 ‘대단히 과식했다'고 응답한 경우가 64.0%로 많은 대상자들이 과식하는 것으로 나타났는데 이로 인한 건강 및 영양문제에 대한 교육이 필요하고 운영면에서는 이러한 일종의 음식의 낭비를 줄일 수 있는 방안에 대한 연구가 필요하다고 사료되었다. 5) 향후 부페식당의 발전방향에 대한 의견 부페식당의 발전방향에 대해 '가지수를 줄여서라도 가격을 짜게 하자'는 의견이 82.9%로 대부분 조사 대상자들이 현재 부페가격에 대해 부정적인 반응을 보였다. '한국음식을 더 많이 해서 전통음식과 친밀한 장소로 발전시키자', '계절식품을 이용하고 비슷한 종류의 음식은 빼서 가격을 낮추자', '연령에 따라서, 또, 성인에서는 성별에 따라 가격 차이를 두자'는 의견 등이 있었다.이용한 배반포의 체외생산에 있어서 배양액내 항산화제의 첨가는 체외성숙단계에서만 효과적이었다. 이것은 아마도 항산화제가 체외성숙 시 난포란 내에서 일어나는 여러 가지 생화학 반응의 처리시간과 관련하여 활성화시킴으로써 난포란의 생존력을 높인 것이라고 사료되기 때문에 앞으로는 돼지 난포란의 효율적인 체외성숙에 대해서 배양액내 첨가물질은 물론 나아가서 방법론적인 측면에서 더욱 연구되어져야 할 것이다.ed from Nusselt's film condensation theory on tilted plate. Using those two expressions, a correlation was formulated as a function of heat flux and tilt angle, to determine the total thermal resistance of a tilted thermosyphon. The correlation formula showed a good agreement with the experimental

• Clinical and Experimental Reproductive Medicine
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• 제50권3호
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• pp.177-184
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• 2023

Objective: Reconstructed oocytes after polar body genome transfer constitute a potential therapeutic option for patients with a history of embryo fragmentation and advanced maternal age. However, the rescue of genetic material from the first polar body (PB1) through introduction into the donor cytoplasm is not yet ready for clinical application. Methods: Eighty-five oocytes were obtained following in vitro maturation (IVM) and divided into two groups: PB1 nuclear transfer (PB1NT; n=54) and control (n=31). Following enucleation and PB1 genomic transfer, PB1 fusion was assessed. Subsequently, all fused oocytes underwent intracytoplasmic sperm injection (ICSI) and were cultured in an incubator under a time-lapse monitoring system to evaluate fertilization, embryonic morphokinetic parameters, and cleavage patterns. Results: Following enucleation and fusion, 77.14% of oocytes survived, and 92.59% of polar bodies (PBs) fused. However, the normal fertilization rate was lower in the PB1NT group than in the control group (56.41% vs. 92%, p=0.002). No significant differences were observed in embryo kinetics between the groups, but a significant difference was detected in embryo developmental arrest after the four-cell stage, along with abnormal cleavage division in the PB1NT group. This was followed by significant between-group differences in the implantation potential rate and euploidy status. Most embryos in the PB1NT group had at least one abnormal cleavage division (93.3%, p=0.001). Conclusion: Fresh PB1NT oocytes successfully produced normal zygotes following PB fusion and ICSI in IVM oocytes. However, this was accompanied by low efficiency in developing into cleavage embryos, along with an increase in abnormal cleavage patterns.