• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.

• Journal of Korean Society of Transportation
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• v.34 no.1
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• pp.68-80
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• 2016

Interest in freeway truck traffic has increased largely due to greater safety concerns regarding truck-related crashes. The negative interactions between slow-moving trucks and other vehicles are a primary cause of hazardous conditions, which lead to crashes with larger speed variations. To improve operational efficiency and safety, providing a climbing lane that separates slow-moving trucks from higher performance vehicles is frequently considered when upgrading geometrics. This study developed an operations strategy for freeway climbing lanes based on traffic conditions in real time. To consider traffic safety when designing a dynamic strategy to determine whether a climbing lane is closed or open, various factors, including the level of service (LOS) and the percentage of trucks, are investigated through microscopic simulations. A microscopic traffic simulator, VISSIM, was used to simulate freeway traffic streams and collect vehicle-maneuvering data. Additionally, an external application program interface, VISSIM's COM-interface, was used to implement the proposed climbing lane operations strategies. Surrogate safety measures (SSM), including the frequency of rear-end conflicts and, were used to quantitatively evaluate the traffic safety using an analysis of individual vehicle trajectories obtained from VISSIM simulations with various operations scenarios. It is expected that the proposed algorithm can be the backbone for operating the climbing lane in real time for safer traffic management.

• International Journal of High-Rise Buildings
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• v.6 no.3
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• pp.227-235
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• 2017

This paper describes some of the challenges for structural design of a mid-story seismic isolated high-rise building, which is located near Tokyo station, completed in 2015. The building is a mixed-use complex and encompasses three volumes: one substructure including basement and lower floors, and a pair of seismic isolated superstructures on the substructure. One is a 136.5m high Main Tower (office use), and the other is a 98.5 m high South Tower (hotel use). The seismic isolation systems are arranged in the $3^{rd}$ floor of the Main Tower and $5^{th}$ floor of the South Tower, so that we call this isolation system as the mid-story seismic isolation. The primary goal of the structural design of this building was to secure high seismic safety against the largest earthquake expected in Tokyo. We adopted optimal seismic isolation equipment simulated by dynamic analysis to minimize building damage. On the other hand, wind-induced vibration of a seismic isolated high-rise building tends to be excited. To reduce the vibration, the following strategies were adopted respectively. In the Main Tower with a large wind receiving area, we adopted a mechanism that locks oil dampers at the isolation level during strong wind. In the South Tower, two tuned mass dampers (TMDs) are installed at the top of the building to control the vibration. In addition, our paper will also report the building performance evaluated for wind and seismic observation after completion of the building. In 2016, an earthquake of seismic intensity 3 (JMA scale) occurred twice in Tokyo. The acceleration reduction rate of the seismic isolation level due to these earthquakes was approximately 30 to 60%. These are also verified by dynamic analysis using observed acceleration data. Also, in April 2016, a strong wind exceeding the speed of 25m/s occurred in Tokyo. On the basis of the record at the strong wind, we confirmed that the locking mechanism of oil damper worked as designed.

• Spatial Information Research
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• v.23 no.1
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• pp.41-48
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• 2015

BIM Technology contains data from the life cycle of facility through 3D modeling. For these, one building products the huge file because of massive data. One of them is IFC which is the standard format, and there are issues that large scale data processing based on geometry and property information of object. It increases the rendering speed and constitutes the graphic card, so large scale data is inefficient for screen visualization to user. The light weighting of large scale BIM data has to solve for process and quality of program essentially. This paper has been searched and confirmed about light weight techniques from domestic and abroad researches. To control and visualize the large scale BIM data effectively, we proposed and verified the technique which is able to optimize the BIM character. For operating the large scale data of facility on web based GIS platform, the quality of screen switch from user phase and the effective memory operation were secured.

• Tunnel and Underground Space
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• v.25 no.5
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• pp.462-469
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• 2015

This study carried out a topographic survey at a small-scale open-pit limestone mine in Korea (the Daesung MDI Seoggyo office) using a popular rotary-wing unmanned aerial vehicle (UAV, Drone, DJI Phantom2 Vision+). 89 sheets of aerial photos could be obtained as a result of performing an automatic flight for 30 minutes under conditions of 100m altitude and 3m/s speed. A total of 34 million cloud points with X, Y, Z-coordinates was extracted from the aerial photos after data processing for correction and matching, then an orthomosaic image and digital surface model with 5m grid spacing could be generated. A comparison of the X, Y, Z-coordinates of 5 ground control points measured by differential global positioning system and those determined by UAV photogrammetry revealed that the root mean squared errors of X, Y, Z-coordinates were around 10cm. Therefore, it is expected that the popular rotary-wing UAV photogrammetry can be effectively utilized in small-scale open-pit mines as a technology that is able to replace or supplement existing topographic surveying equipments.

• The Journal of Korean Academy of Prosthodontics
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• v.40 no.3
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• pp.296-305
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• 2002

The main disadvantage of cement-retained implant restorations is their difficulty in retrievability. Advocates of cemented implant restorations frequently state that retrievability of the restoration can be maintained if a provisional cement is used. The purpose of this study was to find the optimal properties of provisional luting cements and the surface treatment of abutments in single implant abutment system. 30 prefabricated implant abutments, height 8mm, diameter 6mm, 3-degree taper per side, with light chamfer margins were obtained. Three commercially available provisional luting agents which were all zinc oxide eugenol type ; Cavitec, TempBond and TempBond NE were evaluated. No cement served as the control. TempBond along with vaseline, a kind of petrolatum (2:1 ratio) was also evaluated. Ten out of thirty abutments were randomly selected and abutment surfaces were sandblasted with $50{\mu}m$ aluminum oxide. Another ten abutments were sandblasted with $250{\mu}m$ aluminum oxide. A vertical groove, 1 mm deep and 5mm long was cut in each twenty abutments. Ten of them were sandblasted with $50{\mu}m$ aluminum oxide. The full coverage casting crowns were cemented to the abutments with the designated provisional luting agent. Specimens were stored in distilled water at $37^{\circ}C$ for 24 hours. Each specimen was attached to a universal testing machine. A crosshead speed of 0.5mm/min was used to apply a tensile force to each specimen. Within the limitations of this in vitro study, the following conclusions were drawn: 1. Tensile bond strength of provisional luting cements in no surface treatment decreased with the sequence of TempBond NE, TempBond, Cavitec, TempBond with vaseline, no cement. 2. Tensile bond strength more increased by surface treatment. Sandblasting with $250{\mu}m$ aluminum oxide exhibited the highest tensile bond strength in the abutment cemented with TempBond NE and sandblasting with $50{\mu}m$ aluminum oxide exhibited the highest tensile bond strength in cemented with TempBond. 3. In the aspect of a groove formation, tensile bond strength significantly increased in TempBond with vaseline only and the others had no significant effect on tensile bond strength.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.11
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• pp.1309-1315
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• 2014

The switch for a maglev vehicle should be designed such that the vehicle safely changes its track without touching the guiderail. In particular, a medium-to-low-speed EMS -type maglev train relies heavily on a U-type electromagnet where it generates levitation force and guidance force simultaneously. Therefore, it is necessary to evaluate the safety of the vehicle whenever it passes the switch, as it lacks active control of the guidance force. Furthermore, when the vehicle passes a segmented switch, which is a group of curves made up of connected lines with a small radius of curvature, it may come into mechanical contact with the guiderail owing to the excessive lateral displacement of the electromagnet. The goal of this study is to analyze the influence of a segmented switch on the safety of major design-related variables for achieving improved running safety. We propose a three-dimensional multibody dynamics model composed of two cars with one body. Using the proposed model, we perform a simulation of the lateral air gap, which is one of the measurements of the running safety of the vehicle when it passes the switch. The analyzed design variables are the length between short span girder, the articulation angle, the length between two centers of a fixed girder at its ends, and the number of girders. On the basis of the effects of the considered design variables, we establish an optimized design of a switch with improved safety.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.17 no.1
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• pp.28-37
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• 2006

Purpose: Evaluation of vocal cord vibration is very important in cases of voice disorders. There are several equipments for examining the vocal fold vibration such as laryngeal stroboscope, ultra high-speed digital imaging system, and videokymograph. Among these, laryngeal stroboscope is the most popular equipment because of easy to examine the laryngeal pathology. However, current laryngo-stroboscopes are too bulky to move and relatively expensive. The purpose of this research is to develope a portable laryngeal stroboscope of equivalent performance with the current equipments. Methods and Materials: Recently developed high luminescent white LEDs(light emitting diodes) are placed at the head of the endoscope as light sources for the CCD image sensor which is also placed at the head with imaging lens. This arrangement eliminates the bulky light source like expensive halogen or xenon lamps as well as the optical light guiding cables. The LEDs are controlled to flash in phase with the voice frequency of the examinee. The CCD captures these strobo images and converts them into video signals for examinations. Results: There was no functional differences between preexisting stroboscope and the newly developed stroboscope of this study. LED light sources and microprocessor based control circuits of the stroboscope enabled the development of flicker-less, hand-held, portable and battery-operating stroboscope. Conclusion: The developed stroboscope is cost-effective, small-sized, easy to use and very easy desirable to bring and to use in any place.

• Journal of Navigation and Port Research
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• v.30 no.1 s.107
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• pp.9-15
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• 2006

At the adjacent sea area of Busan Central Wharf, a variety of vessels, such as middle-large passenger ships, small hugh speed crafts, container ships, cargo ships and working boats as well as small miscellaneous vessels are freely sailing comparatively without special rules and marine traffic control. In this research, we analyzed traffic stream and navigational characteristics cf main traffic route based on statistics and distribution of tracks by ship's type and tonnage cf the passing vessels after conducting marine traffic survey twice using exclusive software. We examined the traffic safety of the passing vessels by classifying the sea area by each function based on the analysis about this traffic situation, and analyzing the effect by designating 'Inner passage'. We also studied the plan for the effective rearrangement cf Central Wharf considering basically the traffic safety of arrival and departure in a point of view of navigators.

• Journal of Satellite, Information and Communications
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• v.2 no.2
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• pp.64-68
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• 2007

This paper describes the development of RF front.end equipment of a wide band high precision satellite navigation receiver to be able to receive the currently available GPS navigation signal and the GALILEO navigation signal to be developed in Europe in the near future. The wide band satellite navigation receiver with high precision performance is composed of L - band antenna, RF/IF converters for multi - band navigation signals, and high performance baseband processor. The L - band satellite navigation antenna is able to be received the signals in the range from 1.1 GHz to 1.6 GHz and from the navigation satellite positioned near the horizon. The navigation signal of GALILEO navigation satellite consists of L1, E5, and E6 band with signal bandwidth more than 20 MHz which is wider than GPS signal. Due to the wide band navigation signal, the IF frequency and signal processing speed should be increased. The RF/IF converter has been designed with the single stage downconversion structure, and the IF frequency of 140 MHz has been derived from considering the maximum signal bandwidth and the sampling frequency of 112 MHz to be used in ADC circuit. The final output of RF/IF converter is a digital IF signal which is generated from signal processing of the AD converter from the IF signal. The developed RF front - end has the C/N0 performance over 40dB - Hz for the - 130dBm input signal power and includes the automatic gain control circuits to provide the dynamic range over 40dB.